Amino acid dipepetide frequency for Roseburia inulinivorans CAG:15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.113AlaAla: 7.113 ± 0.137	1.028AlaCys: 1.028 ± 0.039	4.822AlaAsp: 4.822 ± 0.08	5.925AlaGlu: 5.925 ± 0.093	3.024AlaPhe: 3.024 ± 0.057	5.984AlaGly: 5.984 ± 0.087	1.094AlaHis: 1.094 ± 0.042	5.187AlaIle: 5.187 ± 0.084	5.064AlaLys: 5.064 ± 0.088	6.545AlaLeu: 6.545 ± 0.09	2.492AlaMet: 2.492 ± 0.051	2.742AlaAsn: 2.742 ± 0.054	1.768AlaPro: 1.768 ± 0.045	2.255AlaGln: 2.255 ± 0.056	2.476AlaArg: 2.476 ± 0.057	3.902AlaSer: 3.902 ± 0.075	3.24AlaThr: 3.24 ± 0.068	6.416AlaVal: 6.416 ± 0.107	0.581AlaTrp: 0.581 ± 0.028	2.945AlaTyr: 2.945 ± 0.062	0.005AlaXaa: 0.005 ± 0.003
Cys	0.966CysAla: 0.966 ± 0.035	0.291CysCys: 0.291 ± 0.018	0.882CysAsp: 0.882 ± 0.036	0.976CysGlu: 0.976 ± 0.036	0.599CysPhe: 0.599 ± 0.025	1.492CysGly: 1.492 ± 0.052	0.31CysHis: 0.31 ± 0.016	1.123CysIle: 1.123 ± 0.041	0.836CysLys: 0.836 ± 0.032	1.055CysLeu: 1.055 ± 0.034	0.482CysMet: 0.482 ± 0.022	0.6CysAsn: 0.6 ± 0.027	0.542CysPro: 0.542 ± 0.031	0.406CysGln: 0.406 ± 0.022	0.574CysArg: 0.574 ± 0.029	0.858CysSer: 0.858 ± 0.031	0.647CysThr: 0.647 ± 0.032	1.072CysVal: 1.072 ± 0.036	0.112CysTrp: 0.112 ± 0.011	0.605CysTyr: 0.605 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	4.506AspAla: 4.506 ± 0.077	0.772AspCys: 0.772 ± 0.026	3.117AspAsp: 3.117 ± 0.073	5.023AspGlu: 5.023 ± 0.08	2.839AspPhe: 2.839 ± 0.063	4.268AspGly: 4.268 ± 0.072	1.018AspHis: 1.018 ± 0.037	4.7AspIle: 4.7 ± 0.072	3.392AspLys: 3.392 ± 0.07	4.448AspLeu: 4.448 ± 0.084	1.954AspMet: 1.954 ± 0.046	2.495AspAsn: 2.495 ± 0.063	1.547AspPro: 1.547 ± 0.045	1.411AspGln: 1.411 ± 0.043	2.145AspArg: 2.145 ± 0.052	3.044AspSer: 3.044 ± 0.059	3.388AspThr: 3.388 ± 0.069	3.891AspVal: 3.891 ± 0.079	0.582AspTrp: 0.582 ± 0.029	3.07AspTyr: 3.07 ± 0.065	0.001AspXaa: 0.001 ± 0.001
Glu	5.599GluAla: 5.599 ± 0.084	0.907GluCys: 0.907 ± 0.032	4.254GluAsp: 4.254 ± 0.078	7.365GluGlu: 7.365 ± 0.115	2.63GluPhe: 2.63 ± 0.052	4.121GluGly: 4.121 ± 0.081	1.602GluHis: 1.602 ± 0.049	5.785GluIle: 5.785 ± 0.089	7.666GluLys: 7.666 ± 0.109	6.965GluLeu: 6.965 ± 0.103	2.6GluMet: 2.6 ± 0.062	4.642GluAsn: 4.642 ± 0.08	1.726GluPro: 1.726 ± 0.052	3.443GluGln: 3.443 ± 0.078	3.264GluArg: 3.264 ± 0.063	3.315GluSer: 3.315 ± 0.065	4.024GluThr: 4.024 ± 0.078	4.869GluVal: 4.869 ± 0.077	0.631GluTrp: 0.631 ± 0.028	3.233GluTyr: 3.233 ± 0.063	0.0GluXaa: 0.0 ± 0.0
Phe	3.097PheAla: 3.097 ± 0.063	0.753PheCys: 0.753 ± 0.029	2.601PheAsp: 2.601 ± 0.056	2.954PheGlu: 2.954 ± 0.062	1.989PhePhe: 1.989 ± 0.053	3.05PheGly: 3.05 ± 0.064	0.866PheHis: 0.866 ± 0.034	2.966PheIle: 2.966 ± 0.068	1.947PheLys: 1.947 ± 0.049	4.049PheLeu: 4.049 ± 0.083	1.279PheMet: 1.279 ± 0.041	1.585PheAsn: 1.585 ± 0.045	1.337PhePro: 1.337 ± 0.039	1.295PheGln: 1.295 ± 0.039	1.533PheArg: 1.533 ± 0.04	2.883PheSer: 2.883 ± 0.059	2.423PheThr: 2.423 ± 0.051	3.121PheVal: 3.121 ± 0.064	0.459PheTrp: 0.459 ± 0.027	1.876PheTyr: 1.876 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	4.693GlyAla: 4.693 ± 0.092	1.208GlyCys: 1.208 ± 0.038	3.424GlyAsp: 3.424 ± 0.064	4.42GlyGlu: 4.42 ± 0.08	3.063GlyPhe: 3.063 ± 0.069	4.18GlyGly: 4.18 ± 0.095	1.165GlyHis: 1.165 ± 0.045	6.055GlyIle: 6.055 ± 0.094	5.475GlyLys: 5.475 ± 0.097	5.437GlyLeu: 5.437 ± 0.093	2.561GlyMet: 2.561 ± 0.053	3.175GlyAsn: 3.175 ± 0.076	1.09GlyPro: 1.09 ± 0.043	1.989GlyGln: 1.989 ± 0.054	2.618GlyArg: 2.618 ± 0.056	3.745GlySer: 3.745 ± 0.083	4.207GlyThr: 4.207 ± 0.08	4.806GlyVal: 4.806 ± 0.078	0.591GlyTrp: 0.591 ± 0.03	3.377GlyTyr: 3.377 ± 0.067	0.002GlyXaa: 0.002 ± 0.002
His	1.186HisAla: 1.186 ± 0.037	0.266HisCys: 0.266 ± 0.018	0.97HisAsp: 0.97 ± 0.043	1.174HisGlu: 1.174 ± 0.04	0.921HisPhe: 0.921 ± 0.033	1.302HisGly: 1.302 ± 0.048	0.46HisHis: 0.46 ± 0.03	1.405HisIle: 1.405 ± 0.045	1.012HisLys: 1.012 ± 0.037	1.495HisLeu: 1.495 ± 0.045	0.591HisMet: 0.591 ± 0.026	0.775HisAsn: 0.775 ± 0.034	0.772HisPro: 0.772 ± 0.032	0.549HisGln: 0.549 ± 0.025	0.754HisArg: 0.754 ± 0.033	0.954HisSer: 0.954 ± 0.038	0.942HisThr: 0.942 ± 0.032	1.161HisVal: 1.161 ± 0.042	0.151HisTrp: 0.151 ± 0.012	0.854HisTyr: 0.854 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.094IleAla: 6.094 ± 0.089	1.303IleCys: 1.303 ± 0.044	4.251IleAsp: 4.251 ± 0.074	5.082IleGlu: 5.082 ± 0.08	3.194IlePhe: 3.194 ± 0.062	5.167IleGly: 5.167 ± 0.09	1.414IleHis: 1.414 ± 0.035	5.367IleIle: 5.367 ± 0.109	4.055IleLys: 4.055 ± 0.081	7.399IleLeu: 7.399 ± 0.114	2.068IleMet: 2.068 ± 0.05	3.051IleAsn: 3.051 ± 0.066	3.097IlePro: 3.097 ± 0.063	2.332IleGln: 2.332 ± 0.056	3.585IleArg: 3.585 ± 0.072	4.888IleSer: 4.888 ± 0.086	4.374IleThr: 4.374 ± 0.08	5.278IleVal: 5.278 ± 0.097	0.624IleTrp: 0.624 ± 0.03	3.084IleTyr: 3.084 ± 0.064	0.0IleXaa: 0.0 ± 0.0
Lys	4.826LysAla: 4.826 ± 0.072	0.724LysCys: 0.724 ± 0.032	3.981LysAsp: 3.981 ± 0.069	6.843LysGlu: 6.843 ± 0.103	2.081LysPhe: 2.081 ± 0.053	3.838LysGly: 3.838 ± 0.066	1.081LysHis: 1.081 ± 0.038	5.426LysIle: 5.426 ± 0.092	6.719LysLys: 6.719 ± 0.11	5.546LysLeu: 5.546 ± 0.086	2.444LysMet: 2.444 ± 0.058	4.119LysAsn: 4.119 ± 0.069	1.892LysPro: 1.892 ± 0.053	2.558LysGln: 2.558 ± 0.057	2.782LysArg: 2.782 ± 0.059	3.207LysSer: 3.207 ± 0.064	3.781LysThr: 3.781 ± 0.07	4.446LysVal: 4.446 ± 0.075	0.565LysTrp: 0.565 ± 0.026	3.095LysTyr: 3.095 ± 0.067	0.0LysXaa: 0.0 ± 0.0
Leu	6.731LeuAla: 6.731 ± 0.099	1.464LeuCys: 1.464 ± 0.04	4.955LeuAsp: 4.955 ± 0.076	6.097LeuGlu: 6.097 ± 0.1	3.934LeuPhe: 3.934 ± 0.08	5.579LeuGly: 5.579 ± 0.09	1.571LeuHis: 1.571 ± 0.05	6.081LeuIle: 6.081 ± 0.12	6.097LeuLys: 6.097 ± 0.089	8.33LeuLeu: 8.33 ± 0.122	2.644LeuMet: 2.644 ± 0.057	3.967LeuAsn: 3.967 ± 0.067	3.195LeuPro: 3.195 ± 0.063	3.032LeuGln: 3.032 ± 0.057	3.32LeuArg: 3.32 ± 0.063	5.868LeuSer: 5.868 ± 0.086	4.971LeuThr: 4.971 ± 0.09	5.37LeuVal: 5.37 ± 0.093	0.702LeuTrp: 0.702 ± 0.032	3.398LeuTyr: 3.398 ± 0.071	0.001LeuXaa: 0.001 ± 0.001
Met	2.469MetAla: 2.469 ± 0.06	0.36MetCys: 0.36 ± 0.023	2.037MetAsp: 2.037 ± 0.053	2.798MetGlu: 2.798 ± 0.058	1.152MetPhe: 1.152 ± 0.033	1.918MetGly: 1.918 ± 0.049	0.467MetHis: 0.467 ± 0.025	2.393MetIle: 2.393 ± 0.051	2.689MetLys: 2.689 ± 0.054	2.824MetLeu: 2.824 ± 0.058	1.038MetMet: 1.038 ± 0.033	1.742MetAsn: 1.742 ± 0.041	1.215MetPro: 1.215 ± 0.043	1.272MetGln: 1.272 ± 0.037	1.219MetArg: 1.219 ± 0.039	1.885MetSer: 1.885 ± 0.049	1.908MetThr: 1.908 ± 0.047	2.071MetVal: 2.071 ± 0.05	0.227MetTrp: 0.227 ± 0.015	0.965MetTyr: 0.965 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	3.411AsnAla: 3.411 ± 0.064	0.671AsnCys: 0.671 ± 0.028	2.345AsnAsp: 2.345 ± 0.051	3.384AsnGlu: 3.384 ± 0.063	1.754AsnPhe: 1.754 ± 0.043	3.606AsnGly: 3.606 ± 0.067	0.904AsnHis: 0.904 ± 0.031	3.846AsnIle: 3.846 ± 0.066	2.666AsnLys: 2.666 ± 0.058	4.0AsnLeu: 4.0 ± 0.069	1.533AsnMet: 1.533 ± 0.052	2.05AsnAsn: 2.05 ± 0.067	1.92AsnPro: 1.92 ± 0.054	1.681AsnGln: 1.681 ± 0.057	1.991AsnArg: 1.991 ± 0.044	2.452AsnSer: 2.452 ± 0.062	2.624AsnThr: 2.624 ± 0.059	3.16AsnVal: 3.16 ± 0.058	0.419AsnTrp: 0.419 ± 0.022	2.056AsnTyr: 2.056 ± 0.057	0.001AsnXaa: 0.001 ± 0.001
Pro	2.24ProAla: 2.24 ± 0.062	0.402ProCys: 0.402 ± 0.019	2.23ProAsp: 2.23 ± 0.055	3.13ProGlu: 3.13 ± 0.074	1.449ProPhe: 1.449 ± 0.048	1.981ProGly: 1.981 ± 0.054	0.472ProHis: 0.472 ± 0.024	2.039ProIle: 2.039 ± 0.052	1.816ProLys: 1.816 ± 0.05	2.457ProLeu: 2.457 ± 0.061	0.933ProMet: 0.933 ± 0.035	1.165ProAsn: 1.165 ± 0.036	0.566ProPro: 0.566 ± 0.03	1.008ProGln: 1.008 ± 0.038	0.831ProArg: 0.831 ± 0.034	1.481ProSer: 1.481 ± 0.039	1.496ProThr: 1.496 ± 0.04	2.76ProVal: 2.76 ± 0.056	0.327ProTrp: 0.327 ± 0.019	1.41ProTyr: 1.41 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.309GlnAla: 2.309 ± 0.059	0.367GlnCys: 0.367 ± 0.021	1.555GlnAsp: 1.555 ± 0.046	2.671GlnGlu: 2.671 ± 0.059	1.281GlnPhe: 1.281 ± 0.043	1.775GlnGly: 1.775 ± 0.045	0.496GlnHis: 0.496 ± 0.024	2.908GlnIle: 2.908 ± 0.071	2.942GlnLys: 2.942 ± 0.063	2.919GlnLeu: 2.919 ± 0.067	1.283GlnMet: 1.283 ± 0.038	1.898GlnAsn: 1.898 ± 0.054	0.889GlnPro: 0.889 ± 0.035	1.233GlnGln: 1.233 ± 0.041	1.205GlnArg: 1.205 ± 0.037	1.698GlnSer: 1.698 ± 0.046	1.9GlnThr: 1.9 ± 0.053	2.017GlnVal: 2.017 ± 0.057	0.284GlnTrp: 0.284 ± 0.019	1.522GlnTyr: 1.522 ± 0.051	0.0GlnXaa: 0.0 ± 0.0
Arg	2.439ArgAla: 2.439 ± 0.053	0.47ArgCys: 0.47 ± 0.027	2.033ArgAsp: 2.033 ± 0.05	3.6ArgGlu: 3.6 ± 0.077	1.73ArgPhe: 1.73 ± 0.046	2.146ArgGly: 2.146 ± 0.053	0.735ArgHis: 0.735 ± 0.031	3.177ArgIle: 3.177 ± 0.062	3.321ArgLys: 3.321 ± 0.064	3.135ArgLeu: 3.135 ± 0.065	1.524ArgMet: 1.524 ± 0.043	2.034ArgAsn: 2.034 ± 0.047	1.14ArgPro: 1.14 ± 0.039	1.547ArgGln: 1.547 ± 0.044	1.874ArgArg: 1.874 ± 0.056	1.927ArgSer: 1.927 ± 0.052	2.013ArgThr: 2.013 ± 0.051	2.41ArgVal: 2.41 ± 0.054	0.31ArgTrp: 0.31 ± 0.019	1.703ArgTyr: 1.703 ± 0.05	0.002ArgXaa: 0.002 ± 0.002
Ser	4.153SerAla: 4.153 ± 0.085	0.765SerCys: 0.765 ± 0.033	3.495SerAsp: 3.495 ± 0.076	3.96SerGlu: 3.96 ± 0.072	2.684SerPhe: 2.684 ± 0.06	4.607SerGly: 4.607 ± 0.067	0.928SerHis: 0.928 ± 0.035	4.068SerIle: 4.068 ± 0.07	3.246SerLys: 3.246 ± 0.063	4.697SerLeu: 4.697 ± 0.07	1.968SerMet: 1.968 ± 0.048	2.359SerAsn: 2.359 ± 0.063	1.46SerPro: 1.46 ± 0.048	1.762SerGln: 1.762 ± 0.048	2.283SerArg: 2.283 ± 0.049	3.32SerSer: 3.32 ± 0.079	2.9SerThr: 2.9 ± 0.067	4.278SerVal: 4.278 ± 0.078	0.481SerTrp: 0.481 ± 0.024	2.521SerTyr: 2.521 ± 0.062	0.0SerXaa: 0.0 ± 0.0
Thr	4.401ThrAla: 4.401 ± 0.089	0.673ThrCys: 0.673 ± 0.027	3.538ThrAsp: 3.538 ± 0.079	4.424ThrGlu: 4.424 ± 0.083	2.279ThrPhe: 2.279 ± 0.058	4.507ThrGly: 4.507 ± 0.074	0.914ThrHis: 0.914 ± 0.033	4.147ThrIle: 4.147 ± 0.081	3.236ThrLys: 3.236 ± 0.066	4.736ThrLeu: 4.736 ± 0.085	1.531ThrMet: 1.531 ± 0.04	2.314ThrAsn: 2.314 ± 0.054	2.07ThrPro: 2.07 ± 0.058	1.424ThrGln: 1.424 ± 0.047	1.78ThrArg: 1.78 ± 0.049	3.018ThrSer: 3.018 ± 0.065	3.142ThrThr: 3.142 ± 0.072	4.415ThrVal: 4.415 ± 0.075	0.464ThrTrp: 0.464 ± 0.028	2.226ThrTyr: 2.226 ± 0.057	0.001ThrXaa: 0.001 ± 0.001
Val	4.915ValAla: 4.915 ± 0.093	1.239ValCys: 1.239 ± 0.04	3.908ValAsp: 3.908 ± 0.062	4.79ValGlu: 4.79 ± 0.064	3.035ValPhe: 3.035 ± 0.069	4.223ValGly: 4.223 ± 0.082	1.114ValHis: 1.114 ± 0.035	5.451ValIle: 5.451 ± 0.083	4.677ValLys: 4.677 ± 0.076	6.529ValLeu: 6.529 ± 0.093	2.13ValMet: 2.13 ± 0.05	3.194ValAsn: 3.194 ± 0.06	2.33ValPro: 2.33 ± 0.055	1.842ValGln: 1.842 ± 0.049	2.717ValArg: 2.717 ± 0.057	4.652ValSer: 4.652 ± 0.072	4.522ValThr: 4.522 ± 0.071	5.064ValVal: 5.064 ± 0.081	0.64ValTrp: 0.64 ± 0.031	2.877ValTyr: 2.877 ± 0.064	0.002ValXaa: 0.002 ± 0.002
Trp	0.51TrpAla: 0.51 ± 0.028	0.13TrpCys: 0.13 ± 0.015	0.517TrpAsp: 0.517 ± 0.024	0.644TrpGlu: 0.644 ± 0.033	0.36TrpPhe: 0.36 ± 0.023	0.595TrpGly: 0.595 ± 0.026	0.15TrpHis: 0.15 ± 0.015	0.653TrpIle: 0.653 ± 0.028	0.669TrpLys: 0.669 ± 0.031	0.768TrpLeu: 0.768 ± 0.033	0.344TrpMet: 0.344 ± 0.022	0.552TrpAsn: 0.552 ± 0.029	0.186TrpPro: 0.186 ± 0.016	0.365TrpGln: 0.365 ± 0.026	0.32TrpArg: 0.32 ± 0.018	0.475TrpSer: 0.475 ± 0.023	0.441TrpThr: 0.441 ± 0.024	0.44TrpVal: 0.44 ± 0.021	0.092TrpTrp: 0.092 ± 0.012	0.392TrpTyr: 0.392 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.948TyrAla: 2.948 ± 0.062	0.634TyrCys: 0.634 ± 0.024	2.695TyrAsp: 2.695 ± 0.059	3.378TyrGlu: 3.378 ± 0.074	2.066TyrPhe: 2.066 ± 0.049	2.941TyrGly: 2.941 ± 0.068	0.931TyrHis: 0.931 ± 0.032	2.93TyrIle: 2.93 ± 0.059	2.431TyrLys: 2.431 ± 0.056	3.972TyrLeu: 3.972 ± 0.073	1.249TyrMet: 1.249 ± 0.036	2.014TyrAsn: 2.014 ± 0.053	1.447TyrPro: 1.447 ± 0.04	1.747TyrGln: 1.747 ± 0.049	2.0TyrArg: 2.0 ± 0.046	2.361TyrSer: 2.361 ± 0.062	2.295TyrThr: 2.295 ± 0.061	2.807TyrVal: 2.807 ± 0.054	0.361TyrTrp: 0.361 ± 0.021	2.094TyrTyr: 2.094 ± 0.06	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.002XaaHis: 0.002 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.002XaaGln: 0.002 ± 0.002	0.002XaaArg: 0.002 ± 0.002	0.002XaaSer: 0.002 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.018XaaXaa: 0.018 ± 0.007

Statistics based on 2653 proteins (844942 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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