Tobacco leaf curl disease associated sequence
Average proteome isoelectric point is 4.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7T3Z7|Q7T3Z7_9VIRU Putative C1 protein OS=Tobacco leaf curl disease associated sequence OX=169691 GN=C1 PE=4 SV=1
MM1 pKa = 7.4 TYY3 pKa = 9.91 EE4 pKa = 4.33 HH5 pKa = 6.63 SHH7 pKa = 4.51 NTMTIKK13 pKa = 10.72 YY14 pKa = 8.52 KK15 pKa = 9.19 NQKK18 pKa = 9.54 GMEE21 pKa = 4.43 FIIDD25 pKa = 3.52 VRR27 pKa = 11.84 LKK29 pKa = 9.83 EE30 pKa = 4.38 DD31 pKa = 3.2 NSILVQIQLFSTGSPALATRR51 pKa = 11.84 KK52 pKa = 9.8 FMIPYY57 pKa = 8.08 GHH59 pKa = 6.53 SGIIPPFNFNTLEE72 pKa = 3.96 QGIHH76 pKa = 5.74 NMLALMYY83 pKa = 9.57 KK84 pKa = 9.74 EE85 pKa = 4.56 SNIGEE90 pKa = 4.57 FKK92 pKa = 10.79 QEE94 pKa = 4.58 DD95 pKa = 4.04 MVEE98 pKa = 4.39 CIDD101 pKa = 3.66 MLMMEE106 pKa = 5.27 EE107 pKa = 4.94 APVIDD112 pKa = 4.34 INVMDD117 pKa = 5.27 AYY119 pKa = 10.57 DD120 pKa = 4.0 VYY122 pKa = 10.56 THH124 pKa = 6.81 SSVV127 pKa = 3.05
Molecular weight: 14.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.789
IPC2_protein 4.914
IPC_protein 4.8
Toseland 4.736
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.914
Wikipedia 4.66
Rodwell 4.698
Grimsley 4.66
Solomon 4.787
Lehninger 4.736
Nozaki 4.914
DTASelect 5.041
Thurlkill 4.736
EMBOSS 4.698
Sillero 4.978
Patrickios 3.503
IPC_peptide 4.787
IPC2_peptide 4.978
IPC2.peptide.svr19 4.881
Protein with the highest isoelectric point:
>tr|Q7T3Z7|Q7T3Z7_9VIRU Putative C1 protein OS=Tobacco leaf curl disease associated sequence OX=169691 GN=C1 PE=4 SV=1
MM1 pKa = 7.4 TYY3 pKa = 9.91 EE4 pKa = 4.33 HH5 pKa = 6.63 SHH7 pKa = 4.51 NTMTIKK13 pKa = 10.72 YY14 pKa = 8.52 KK15 pKa = 9.19 NQKK18 pKa = 9.54 GMEE21 pKa = 4.43 FIIDD25 pKa = 3.52 VRR27 pKa = 11.84 LKK29 pKa = 9.83 EE30 pKa = 4.38 DD31 pKa = 3.2 NSILVQIQLFSTGSPALATRR51 pKa = 11.84 KK52 pKa = 9.8 FMIPYY57 pKa = 8.08 GHH59 pKa = 6.53 SGIIPPFNFNTLEE72 pKa = 3.96 QGIHH76 pKa = 5.74 NMLALMYY83 pKa = 9.57 KK84 pKa = 9.74 EE85 pKa = 4.56 SNIGEE90 pKa = 4.57 FKK92 pKa = 10.79 QEE94 pKa = 4.58 DD95 pKa = 4.04 MVEE98 pKa = 4.39 CIDD101 pKa = 3.66 MLMMEE106 pKa = 5.27 EE107 pKa = 4.94 APVIDD112 pKa = 4.34 INVMDD117 pKa = 5.27 AYY119 pKa = 10.57 DD120 pKa = 4.0 VYY122 pKa = 10.56 THH124 pKa = 6.81 SSVV127 pKa = 3.05
Molecular weight: 14.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.789
IPC2_protein 4.914
IPC_protein 4.8
Toseland 4.736
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.914
Wikipedia 4.66
Rodwell 4.698
Grimsley 4.66
Solomon 4.787
Lehninger 4.736
Nozaki 4.914
DTASelect 5.041
Thurlkill 4.736
EMBOSS 4.698
Sillero 4.978
Patrickios 3.503
IPC_peptide 4.787
IPC2_peptide 4.978
IPC2.peptide.svr19 4.881
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
127
127
127
127.0
14.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.937 ± 0.0
0.787 ± 0.0
5.512 ± 0.0
7.874 ± 0.0
4.724 ± 0.0
4.724 ± 0.0
3.937 ± 0.0
10.236 ± 0.0
5.512 ± 0.0
6.299 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
8.661 ± 0.0
6.299 ± 0.0
3.937 ± 0.0
3.937 ± 0.0
1.575 ± 0.0
6.299 ± 0.0
5.512 ± 0.0
5.512 ± 0.0
0.0 ± 0.0
4.724 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here