Rhynchosia yellow mosaic India virus
Average proteome isoelectric point is 7.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7CWL3|E7CWL3_9GEMI Capsid protein OS=Rhynchosia yellow mosaic India virus OX=935473 GN=AV1 PE=3 SV=1
MM1 pKa = 8.02 DD2 pKa = 4.49 LHH4 pKa = 6.74 NHH6 pKa = 5.38 VEE8 pKa = 4.33 HH9 pKa = 6.11 QVSDD13 pKa = 4.18 TIDD16 pKa = 3.59 KK17 pKa = 10.81 NVVSSTSDD25 pKa = 3.18 DD26 pKa = 3.55 SSNAFSLLCLGHH38 pKa = 6.66 WMQVQVVPLGCRR50 pKa = 11.84 SSIFDD55 pKa = 3.75 EE56 pKa = 4.28 LLIAVMRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 YY66 pKa = 9.98 RR67 pKa = 11.84 NSALAWSLEE76 pKa = 4.08 RR77 pKa = 11.84 KK78 pKa = 9.77 FEE80 pKa = 3.77 MSLRR84 pKa = 11.84 KK85 pKa = 9.75 NSNPNEE91 pKa = 3.81 LLGYY95 pKa = 10.43 CSS97 pKa = 3.56
Molecular weight: 11.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.079
IPC2_protein 6.008
IPC_protein 6.122
Toseland 6.427
ProMoST 6.427
Dawson 6.465
Bjellqvist 6.389
Wikipedia 6.491
Rodwell 6.465
Grimsley 6.547
Solomon 6.478
Lehninger 6.478
Nozaki 6.707
DTASelect 6.912
Thurlkill 6.942
EMBOSS 6.927
Sillero 6.854
Patrickios 4.202
IPC_peptide 6.491
IPC2_peptide 6.81
IPC2.peptide.svr19 6.718
Protein with the highest isoelectric point:
>tr|E7CWL4|E7CWL4_9GEMI Transcriptional activator protein OS=Rhynchosia yellow mosaic India virus OX=935473 GN=AC2 PE=3 SV=1
MM1 pKa = 8.06 PKK3 pKa = 10.14 RR4 pKa = 11.84 NYY6 pKa = 8.54 DD7 pKa = 3.61 TAFSSPLSVVRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.26 SPMALPAPASSAQGTTRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 WTNRR48 pKa = 11.84 PMWRR52 pKa = 11.84 KK53 pKa = 8.19 PRR55 pKa = 11.84 FYY57 pKa = 10.75 RR58 pKa = 11.84 LYY60 pKa = 9.25 RR61 pKa = 11.84 TPDD64 pKa = 3.1 VPRR67 pKa = 11.84 GCEE70 pKa = 4.34 GPCKK74 pKa = 10.21 VQSFEE79 pKa = 3.84 QRR81 pKa = 11.84 HH82 pKa = 6.27 DD83 pKa = 3.18 IAHH86 pKa = 6.13 TGKK89 pKa = 10.45 VICISDD95 pKa = 3.51 VTRR98 pKa = 11.84 GNGITHH104 pKa = 6.78 RR105 pKa = 11.84 VGKK108 pKa = 9.44 RR109 pKa = 11.84 FCVKK113 pKa = 9.96 SVYY116 pKa = 8.94 ITGKK120 pKa = 9.34 VWMDD124 pKa = 3.29 EE125 pKa = 4.15 NIKK128 pKa = 10.71 SKK130 pKa = 10.61 NHH132 pKa = 5.84 TNTVMFKK139 pKa = 10.21 LCRR142 pKa = 11.84 DD143 pKa = 3.38 RR144 pKa = 11.84 RR145 pKa = 11.84 PFGTPMDD152 pKa = 4.58 FGQVFNMYY160 pKa = 10.66 DD161 pKa = 3.86 NEE163 pKa = 4.34 PSTATVKK170 pKa = 10.85 NDD172 pKa = 2.82 LRR174 pKa = 11.84 DD175 pKa = 3.34 RR176 pKa = 11.84 FQVLRR181 pKa = 11.84 RR182 pKa = 11.84 FNATVTGGQYY192 pKa = 10.63 ACKK195 pKa = 8.94 EE196 pKa = 3.74 QAMVNRR202 pKa = 11.84 FYY204 pKa = 11.0 RR205 pKa = 11.84 VNNHH209 pKa = 4.66 VVYY212 pKa = 10.63 NHH214 pKa = 5.84 QEE216 pKa = 3.45 AAKK219 pKa = 10.4 YY220 pKa = 9.25 EE221 pKa = 4.13 NHH223 pKa = 6.46 TEE225 pKa = 3.99 NALLLYY231 pKa = 7.29 MACTHH236 pKa = 7.07 ASNPVYY242 pKa = 9.86 ATLKK246 pKa = 9.47 IRR248 pKa = 11.84 IYY250 pKa = 10.69 FYY252 pKa = 11.26 DD253 pKa = 3.31 SVGNN257 pKa = 3.81
Molecular weight: 29.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.531
IPC_protein 9.94
Toseland 10.072
ProMoST 9.853
Dawson 10.306
Bjellqvist 10.043
Wikipedia 10.511
Rodwell 10.555
Grimsley 10.394
Solomon 10.35
Lehninger 10.292
Nozaki 10.131
DTASelect 10.028
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.233
Patrickios 9.882
IPC_peptide 10.335
IPC2_peptide 9.107
IPC2.peptide.svr19 8.466
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1656
97
362
207.0
23.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.133 ± 0.601
2.355 ± 0.349
5.193 ± 0.431
5.133 ± 0.612
4.891 ± 0.26
4.589 ± 0.491
2.717 ± 0.447
5.857 ± 0.606
6.763 ± 0.733
7.246 ± 0.701
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.14 ± 0.6
6.401 ± 0.383
5.012 ± 0.313
4.046 ± 0.512
6.944 ± 1.069
8.937 ± 0.713
5.435 ± 0.51
5.374 ± 0.751
1.147 ± 0.12
3.684 ± 0.46
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here