Amino acid dipepetide frequency for Pseudomonas phage UNO-SLW1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.186AlaAla: 11.186 ± 1.319
0.653AlaCys: 0.653 ± 0.318
6.124AlaAsp: 6.124 ± 0.997
6.124AlaGlu: 6.124 ± 0.655
3.429AlaPhe: 3.429 ± 0.496
7.757AlaGly: 7.757 ± 0.79
2.205AlaHis: 2.205 ± 0.426
3.674AlaIle: 3.674 ± 0.627
6.287AlaLys: 6.287 ± 0.518
8.818AlaLeu: 8.818 ± 1.162
3.511AlaMet: 3.511 ± 0.701
4.083AlaAsn: 4.083 ± 0.655
3.511AlaPro: 3.511 ± 0.624
5.716AlaGln: 5.716 ± 0.942
6.287AlaArg: 6.287 ± 0.691
7.185AlaSer: 7.185 ± 0.86
4.981AlaThr: 4.981 ± 1.063
6.696AlaVal: 6.696 ± 0.66
1.306AlaTrp: 1.306 ± 0.366
3.266AlaTyr: 3.266 ± 0.483
0.0AlaXaa: 0.0 ± 0.0
Cys
1.143CysAla: 1.143 ± 0.295
0.0CysCys: 0.0 ± 0.0
0.653CysAsp: 0.653 ± 0.274
0.49CysGlu: 0.49 ± 0.16
0.408CysPhe: 0.408 ± 0.22
0.817CysGly: 0.817 ± 0.285
0.163CysHis: 0.163 ± 0.099
0.245CysIle: 0.245 ± 0.123
0.572CysLys: 0.572 ± 0.256
1.061CysLeu: 1.061 ± 0.29
0.245CysMet: 0.245 ± 0.135
0.082CysAsn: 0.082 ± 0.096
0.163CysPro: 0.163 ± 0.121
0.408CysGln: 0.408 ± 0.175
0.572CysArg: 0.572 ± 0.211
0.327CysSer: 0.327 ± 0.14
0.327CysThr: 0.327 ± 0.165
0.327CysVal: 0.327 ± 0.167
0.163CysTrp: 0.163 ± 0.105
0.245CysTyr: 0.245 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
6.451AspAla: 6.451 ± 0.671
0.408AspCys: 0.408 ± 0.152
4.654AspAsp: 4.654 ± 0.621
3.593AspGlu: 3.593 ± 0.448
3.103AspPhe: 3.103 ± 0.456
6.777AspGly: 6.777 ± 0.756
1.47AspHis: 1.47 ± 0.405
3.429AspIle: 3.429 ± 0.378
3.184AspLys: 3.184 ± 0.5
4.083AspLeu: 4.083 ± 0.582
1.878AspMet: 1.878 ± 0.398
1.47AspAsn: 1.47 ± 0.294
3.184AspPro: 3.184 ± 0.587
2.368AspGln: 2.368 ± 0.313
3.838AspArg: 3.838 ± 0.71
3.021AspSer: 3.021 ± 0.648
3.103AspThr: 3.103 ± 0.511
4.001AspVal: 4.001 ± 0.658
1.225AspTrp: 1.225 ± 0.357
1.715AspTyr: 1.715 ± 0.276
0.0AspXaa: 0.0 ± 0.0
Glu
8.574GluAla: 8.574 ± 0.859
0.817GluCys: 0.817 ± 0.345
4.083GluAsp: 4.083 ± 0.401
5.062GluGlu: 5.062 ± 1.031
2.45GluPhe: 2.45 ± 0.442
5.307GluGly: 5.307 ± 0.703
1.551GluHis: 1.551 ± 0.343
2.858GluIle: 2.858 ± 0.509
3.348GluLys: 3.348 ± 0.522
5.716GluLeu: 5.716 ± 0.804
1.96GluMet: 1.96 ± 0.426
2.776GluAsn: 2.776 ± 0.367
2.205GluPro: 2.205 ± 0.393
2.368GluGln: 2.368 ± 0.379
3.756GluArg: 3.756 ± 0.5
3.511GluSer: 3.511 ± 0.545
4.409GluThr: 4.409 ± 0.543
4.164GluVal: 4.164 ± 0.626
1.388GluTrp: 1.388 ± 0.37
2.123GluTyr: 2.123 ± 0.403
0.0GluXaa: 0.0 ± 0.0
Phe
2.041PheAla: 2.041 ± 0.498
0.49PheCys: 0.49 ± 0.253
3.021PheAsp: 3.021 ± 0.6
2.123PheGlu: 2.123 ± 0.347
1.388PhePhe: 1.388 ± 0.304
2.939PheGly: 2.939 ± 0.421
0.817PheHis: 0.817 ± 0.236
1.306PheIle: 1.306 ± 0.37
2.123PheLys: 2.123 ± 0.464
3.674PheLeu: 3.674 ± 0.542
1.306PheMet: 1.306 ± 0.25
1.878PheAsn: 1.878 ± 0.414
1.551PhePro: 1.551 ± 0.423
0.735PheGln: 0.735 ± 0.187
2.368PheArg: 2.368 ± 0.396
1.633PheSer: 1.633 ± 0.339
2.531PheThr: 2.531 ± 0.352
2.123PheVal: 2.123 ± 0.451
0.572PheTrp: 0.572 ± 0.207
0.817PheTyr: 0.817 ± 0.372
0.0PheXaa: 0.0 ± 0.0
Gly
7.594GlyAla: 7.594 ± 1.068
0.898GlyCys: 0.898 ± 0.256
4.899GlyAsp: 4.899 ± 0.508
5.552GlyGlu: 5.552 ± 0.638
3.756GlyPhe: 3.756 ± 0.707
7.104GlyGly: 7.104 ± 0.855
1.633GlyHis: 1.633 ± 0.306
4.491GlyIle: 4.491 ± 0.678
4.491GlyLys: 4.491 ± 0.846
6.94GlyLeu: 6.94 ± 0.785
2.041GlyMet: 2.041 ± 0.522
3.103GlyAsn: 3.103 ± 0.489
2.45GlyPro: 2.45 ± 0.444
2.531GlyGln: 2.531 ± 0.431
5.552GlyArg: 5.552 ± 0.629
5.471GlySer: 5.471 ± 0.653
4.164GlyThr: 4.164 ± 0.509
5.389GlyVal: 5.389 ± 0.607
1.47GlyTrp: 1.47 ± 0.304
3.021GlyTyr: 3.021 ± 0.614
0.0GlyXaa: 0.0 ± 0.0
His
1.551HisAla: 1.551 ± 0.328
0.408HisCys: 0.408 ± 0.174
1.225HisAsp: 1.225 ± 0.402
1.715HisGlu: 1.715 ± 0.414
0.898HisPhe: 0.898 ± 0.289
1.878HisGly: 1.878 ± 0.441
0.49HisHis: 0.49 ± 0.198
0.898HisIle: 0.898 ± 0.235
1.306HisLys: 1.306 ± 0.347
2.286HisLeu: 2.286 ± 0.366
0.49HisMet: 0.49 ± 0.181
0.98HisAsn: 0.98 ± 0.296
0.735HisPro: 0.735 ± 0.265
0.735HisGln: 0.735 ± 0.175
1.388HisArg: 1.388 ± 0.306
1.061HisSer: 1.061 ± 0.249
1.143HisThr: 1.143 ± 0.257
1.633HisVal: 1.633 ± 0.373
0.653HisTrp: 0.653 ± 0.205
0.898HisTyr: 0.898 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
3.429IleAla: 3.429 ± 0.539
0.572IleCys: 0.572 ± 0.193
2.939IleAsp: 2.939 ± 0.537
3.674IleGlu: 3.674 ± 0.524
0.898IlePhe: 0.898 ± 0.282
3.266IleGly: 3.266 ± 0.582
0.898IleHis: 0.898 ± 0.325
2.286IleIle: 2.286 ± 0.44
2.858IleLys: 2.858 ± 0.541
3.838IleLeu: 3.838 ± 0.456
1.47IleMet: 1.47 ± 0.371
1.306IleAsn: 1.306 ± 0.31
2.45IlePro: 2.45 ± 0.469
2.123IleGln: 2.123 ± 0.5
3.103IleArg: 3.103 ± 0.299
1.96IleSer: 1.96 ± 0.309
2.776IleThr: 2.776 ± 0.397
3.184IleVal: 3.184 ± 0.466
0.327IleTrp: 0.327 ± 0.143
1.715IleTyr: 1.715 ± 0.472
0.0IleXaa: 0.0 ± 0.0
Lys
8.247LysAla: 8.247 ± 0.913
0.49LysCys: 0.49 ± 0.179
4.246LysAsp: 4.246 ± 0.511
3.919LysGlu: 3.919 ± 0.602
2.531LysPhe: 2.531 ± 0.345
4.899LysGly: 4.899 ± 0.807
1.715LysHis: 1.715 ± 0.459
2.368LysIle: 2.368 ± 0.386
3.184LysLys: 3.184 ± 0.897
4.981LysLeu: 4.981 ± 0.638
1.715LysMet: 1.715 ± 0.403
1.96LysAsn: 1.96 ± 0.455
2.531LysPro: 2.531 ± 0.493
2.205LysGln: 2.205 ± 0.43
3.348LysArg: 3.348 ± 0.655
2.531LysSer: 2.531 ± 0.353
3.511LysThr: 3.511 ± 0.569
3.756LysVal: 3.756 ± 0.696
0.572LysTrp: 0.572 ± 0.182
1.143LysTyr: 1.143 ± 0.267
0.0LysXaa: 0.0 ± 0.0
Leu
8.41LeuAla: 8.41 ± 1.126
0.163LeuCys: 0.163 ± 0.124
5.062LeuAsp: 5.062 ± 0.668
6.287LeuGlu: 6.287 ± 0.712
2.368LeuPhe: 2.368 ± 0.458
5.307LeuGly: 5.307 ± 0.718
2.041LeuHis: 2.041 ± 0.382
3.674LeuIle: 3.674 ± 0.538
7.104LeuLys: 7.104 ± 0.662
5.879LeuLeu: 5.879 ± 0.833
2.695LeuMet: 2.695 ± 0.448
3.919LeuAsn: 3.919 ± 0.572
2.858LeuPro: 2.858 ± 0.459
4.083LeuGln: 4.083 ± 0.484
4.409LeuArg: 4.409 ± 0.492
4.981LeuSer: 4.981 ± 0.637
5.226LeuThr: 5.226 ± 0.723
5.879LeuVal: 5.879 ± 0.576
1.061LeuTrp: 1.061 ± 0.264
1.878LeuTyr: 1.878 ± 0.348
0.0LeuXaa: 0.0 ± 0.0
Met
4.001MetAla: 4.001 ± 0.488
0.163MetCys: 0.163 ± 0.139
1.633MetAsp: 1.633 ± 0.399
2.531MetGlu: 2.531 ± 0.501
0.327MetPhe: 0.327 ± 0.158
2.531MetGly: 2.531 ± 0.486
0.572MetHis: 0.572 ± 0.193
1.388MetIle: 1.388 ± 0.268
1.225MetLys: 1.225 ± 0.311
2.531MetLeu: 2.531 ± 0.484
0.653MetMet: 0.653 ± 0.205
1.143MetAsn: 1.143 ± 0.323
1.47MetPro: 1.47 ± 0.355
0.817MetGln: 0.817 ± 0.223
1.225MetArg: 1.225 ± 0.305
2.286MetSer: 2.286 ± 0.338
2.123MetThr: 2.123 ± 0.429
2.041MetVal: 2.041 ± 0.337
0.327MetTrp: 0.327 ± 0.143
0.408MetTyr: 0.408 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
3.593AsnAla: 3.593 ± 0.503
0.163AsnCys: 0.163 ± 0.12
1.96AsnAsp: 1.96 ± 0.363
2.286AsnGlu: 2.286 ± 0.471
2.286AsnPhe: 2.286 ± 0.333
3.919AsnGly: 3.919 ± 0.555
0.898AsnHis: 0.898 ± 0.314
1.551AsnIle: 1.551 ± 0.483
1.633AsnLys: 1.633 ± 0.324
4.164AsnLeu: 4.164 ± 0.498
1.143AsnMet: 1.143 ± 0.291
1.061AsnAsn: 1.061 ± 0.349
2.776AsnPro: 2.776 ± 0.45
0.98AsnGln: 0.98 ± 0.221
2.531AsnArg: 2.531 ± 0.476
0.817AsnSer: 0.817 ± 0.367
2.45AsnThr: 2.45 ± 0.484
2.776AsnVal: 2.776 ± 0.455
0.245AsnTrp: 0.245 ± 0.146
1.551AsnTyr: 1.551 ± 0.455
0.0AsnXaa: 0.0 ± 0.0
Pro
2.858ProAla: 2.858 ± 0.523
0.245ProCys: 0.245 ± 0.152
3.919ProAsp: 3.919 ± 0.459
3.756ProGlu: 3.756 ± 0.679
1.47ProPhe: 1.47 ± 0.295
2.531ProGly: 2.531 ± 0.378
0.898ProHis: 0.898 ± 0.286
1.388ProIle: 1.388 ± 0.378
2.613ProLys: 2.613 ± 0.45
2.205ProLeu: 2.205 ± 0.415
0.98ProMet: 0.98 ± 0.26
2.368ProAsn: 2.368 ± 0.424
1.388ProPro: 1.388 ± 0.416
1.47ProGln: 1.47 ± 0.306
2.041ProArg: 2.041 ± 0.359
2.205ProSer: 2.205 ± 0.443
2.286ProThr: 2.286 ± 0.449
3.266ProVal: 3.266 ± 0.55
0.572ProTrp: 0.572 ± 0.218
0.898ProTyr: 0.898 ± 0.325
0.0ProXaa: 0.0 ± 0.0
Gln
5.797GlnAla: 5.797 ± 1.031
0.082GlnCys: 0.082 ± 0.079
2.45GlnAsp: 2.45 ± 0.503
2.205GlnGlu: 2.205 ± 0.455
2.123GlnPhe: 2.123 ± 0.423
3.593GlnGly: 3.593 ± 0.58
0.408GlnHis: 0.408 ± 0.167
2.205GlnIle: 2.205 ± 0.352
1.96GlnLys: 1.96 ± 0.56
3.919GlnLeu: 3.919 ± 0.462
1.551GlnMet: 1.551 ± 0.414
1.061GlnAsn: 1.061 ± 0.24
0.898GlnPro: 0.898 ± 0.266
1.796GlnGln: 1.796 ± 0.296
2.368GlnArg: 2.368 ± 0.523
1.878GlnSer: 1.878 ± 0.37
1.551GlnThr: 1.551 ± 0.373
2.776GlnVal: 2.776 ± 0.416
0.653GlnTrp: 0.653 ± 0.205
1.633GlnTyr: 1.633 ± 0.427
0.0GlnXaa: 0.0 ± 0.0
Arg
6.124ArgAla: 6.124 ± 0.784
0.572ArgCys: 0.572 ± 0.264
3.429ArgAsp: 3.429 ± 0.565
4.246ArgGlu: 4.246 ± 0.713
1.633ArgPhe: 1.633 ± 0.318
5.144ArgGly: 5.144 ± 0.546
1.47ArgHis: 1.47 ± 0.434
2.858ArgIle: 2.858 ± 0.366
3.184ArgLys: 3.184 ± 0.521
4.818ArgLeu: 4.818 ± 0.626
1.715ArgMet: 1.715 ± 0.449
3.103ArgAsn: 3.103 ± 0.483
2.45ArgPro: 2.45 ± 0.438
3.266ArgGln: 3.266 ± 0.456
3.348ArgArg: 3.348 ± 0.503
3.184ArgSer: 3.184 ± 0.498
2.368ArgThr: 2.368 ± 0.364
3.511ArgVal: 3.511 ± 0.518
0.653ArgTrp: 0.653 ± 0.186
1.96ArgTyr: 1.96 ± 0.293
0.0ArgXaa: 0.0 ± 0.0
Ser
5.389SerAla: 5.389 ± 0.846
0.572SerCys: 0.572 ± 0.164
3.429SerAsp: 3.429 ± 0.455
2.776SerGlu: 2.776 ± 0.383
2.205SerPhe: 2.205 ± 0.358
5.144SerGly: 5.144 ± 0.679
1.306SerHis: 1.306 ± 0.325
2.613SerIle: 2.613 ± 0.386
3.266SerLys: 3.266 ± 0.558
4.573SerLeu: 4.573 ± 0.636
1.47SerMet: 1.47 ± 0.336
2.123SerAsn: 2.123 ± 0.521
1.96SerPro: 1.96 ± 0.325
1.878SerGln: 1.878 ± 0.426
3.103SerArg: 3.103 ± 0.47
2.613SerSer: 2.613 ± 0.469
2.368SerThr: 2.368 ± 0.413
3.674SerVal: 3.674 ± 0.628
0.898SerTrp: 0.898 ± 0.238
1.96SerTyr: 1.96 ± 0.361
0.0SerXaa: 0.0 ± 0.0
Thr
5.062ThrAla: 5.062 ± 0.487
0.572ThrCys: 0.572 ± 0.199
3.021ThrAsp: 3.021 ± 0.492
4.899ThrGlu: 4.899 ± 0.582
1.47ThrPhe: 1.47 ± 0.437
5.144ThrGly: 5.144 ± 0.755
1.225ThrHis: 1.225 ± 0.216
3.266ThrIle: 3.266 ± 0.413
4.328ThrLys: 4.328 ± 0.539
5.552ThrLeu: 5.552 ± 0.639
1.143ThrMet: 1.143 ± 0.263
2.123ThrAsn: 2.123 ± 0.424
3.103ThrPro: 3.103 ± 0.516
2.45ThrGln: 2.45 ± 0.515
2.45ThrArg: 2.45 ± 0.562
2.531ThrSer: 2.531 ± 0.539
3.511ThrThr: 3.511 ± 0.742
3.919ThrVal: 3.919 ± 0.734
0.408ThrTrp: 0.408 ± 0.203
1.225ThrTyr: 1.225 ± 0.258
0.0ThrXaa: 0.0 ± 0.0
Val
6.777ValAla: 6.777 ± 0.787
0.49ValCys: 0.49 ± 0.182
3.429ValAsp: 3.429 ± 0.516
4.573ValGlu: 4.573 ± 0.659
1.633ValPhe: 1.633 ± 0.397
4.899ValGly: 4.899 ± 0.55
1.47ValHis: 1.47 ± 0.27
2.939ValIle: 2.939 ± 0.443
4.409ValLys: 4.409 ± 0.679
4.654ValLeu: 4.654 ± 0.566
1.47ValMet: 1.47 ± 0.368
2.695ValAsn: 2.695 ± 0.573
2.45ValPro: 2.45 ± 0.396
2.939ValGln: 2.939 ± 0.417
4.736ValArg: 4.736 ± 0.705
3.919ValSer: 3.919 ± 0.482
5.634ValThr: 5.634 ± 0.72
4.573ValVal: 4.573 ± 0.703
1.388ValTrp: 1.388 ± 0.432
1.96ValTyr: 1.96 ± 0.495
0.0ValXaa: 0.0 ± 0.0
Trp
1.551TrpAla: 1.551 ± 0.396
0.163TrpCys: 0.163 ± 0.147
0.817TrpAsp: 0.817 ± 0.214
0.572TrpGlu: 0.572 ± 0.193
0.49TrpPhe: 0.49 ± 0.235
0.98TrpGly: 0.98 ± 0.203
0.653TrpHis: 0.653 ± 0.228
0.572TrpIle: 0.572 ± 0.205
1.306TrpLys: 1.306 ± 0.361
1.715TrpLeu: 1.715 ± 0.387
0.49TrpMet: 0.49 ± 0.199
0.572TrpAsn: 0.572 ± 0.253
0.408TrpPro: 0.408 ± 0.185
0.653TrpGln: 0.653 ± 0.232
0.49TrpArg: 0.49 ± 0.172
1.061TrpSer: 1.061 ± 0.359
0.653TrpThr: 0.653 ± 0.22
1.306TrpVal: 1.306 ± 0.291
0.082TrpTrp: 0.082 ± 0.086
0.163TrpTyr: 0.163 ± 0.099
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.021TyrAla: 3.021 ± 0.697
0.49TyrCys: 0.49 ± 0.199
1.96TyrAsp: 1.96 ± 0.354
2.123TyrGlu: 2.123 ± 0.452
0.572TyrPhe: 0.572 ± 0.213
2.613TyrGly: 2.613 ± 0.371
0.49TyrHis: 0.49 ± 0.2
1.061TyrIle: 1.061 ± 0.23
1.47TyrLys: 1.47 ± 0.373
1.878TyrLeu: 1.878 ± 0.346
1.388TyrMet: 1.388 ± 0.324
0.98TyrAsn: 0.98 ± 0.285
0.817TyrPro: 0.817 ± 0.245
1.388TyrGln: 1.388 ± 0.356
2.123TyrArg: 2.123 ± 0.317
1.143TyrSer: 1.143 ± 0.281
2.286TyrThr: 2.286 ± 0.541
2.041TyrVal: 2.041 ± 0.393
0.735TyrTrp: 0.735 ± 0.226
0.327TyrTyr: 0.327 ± 0.17
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (12248 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski