archaeon HR06
Average proteome isoelectric point is 7.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1118 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5VFK0|A0A2H5VFK0_9ARCH Inner membrane ABC transporter permease protein YcjP OS=archaeon HR06 OX=2035442 GN=ycjP_4 PE=3 SV=1
MM1 pKa = 7.89 SLEE4 pKa = 4.47 CLDD7 pKa = 4.36 CGGSIKK13 pKa = 10.23 IPKK16 pKa = 9.28 DD17 pKa = 3.61 AIAGEE22 pKa = 4.48 VLTCPDD28 pKa = 4.2 CGSSFEE34 pKa = 4.33 LVKK37 pKa = 10.88 DD38 pKa = 3.71 EE39 pKa = 5.64 KK40 pKa = 11.32 GFSLKK45 pKa = 10.02 FAQVEE50 pKa = 4.32 GEE52 pKa = 4.2 DD53 pKa = 3.28 WGEE56 pKa = 3.68
Molecular weight: 5.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.252
IPC2_protein 4.19
IPC_protein 4.037
Toseland 3.872
ProMoST 4.113
Dawson 3.999
Bjellqvist 4.253
Wikipedia 3.91
Rodwell 3.872
Grimsley 3.783
Solomon 3.986
Lehninger 3.935
Nozaki 4.126
DTASelect 4.266
Thurlkill 3.91
EMBOSS 3.923
Sillero 4.151
Patrickios 3.465
IPC_peptide 3.986
IPC2_peptide 4.139
IPC2.peptide.svr19 4.071
Protein with the highest isoelectric point:
>tr|A0A2H5VEL1|A0A2H5VEL1_9ARCH Bifunctional protein HldE OS=archaeon HR06 OX=2035442 GN=hldE_1 PE=4 SV=1
MM1 pKa = 7.15 PTHH4 pKa = 6.98 GSLTKK9 pKa = 10.14 AGKK12 pKa = 9.72 VRR14 pKa = 11.84 SQTPKK19 pKa = 8.54 IPARR23 pKa = 11.84 PRR25 pKa = 11.84 HH26 pKa = 5.32 SPIPKK31 pKa = 9.21 VRR33 pKa = 11.84 LKK35 pKa = 11.05 SIFVKK40 pKa = 10.65 RR41 pKa = 11.84 NILKK45 pKa = 10.4 RR46 pKa = 11.84 KK47 pKa = 9.19 PGQNWINVV55 pKa = 3.56
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 10.994
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.618
Grimsley 12.764
Solomon 13.203
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.34
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 9.019
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1118
0
1118
263615
29
1009
235.8
26.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.583 ± 0.076
0.977 ± 0.032
4.762 ± 0.045
7.955 ± 0.081
4.81 ± 0.075
6.503 ± 0.058
1.303 ± 0.026
9.199 ± 0.069
9.522 ± 0.096
11.755 ± 0.11
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.056 ± 0.03
4.132 ± 0.048
3.859 ± 0.052
1.676 ± 0.034
4.718 ± 0.055
5.926 ± 0.063
3.961 ± 0.074
6.284 ± 0.067
1.044 ± 0.034
3.974 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here