Corticimicrobacter populi
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3237 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V1JXE6|A0A2V1JXE6_9BURK Metal ABC transporter substrate-binding protein OS=Corticimicrobacter populi OX=2175229 GN=DD235_13970 PE=3 SV=1
MM1 pKa = 7.13 WCVPRR6 pKa = 11.84 YY7 pKa = 9.23 LVQSTEE13 pKa = 4.2 DD14 pKa = 3.7 GSFLAADD21 pKa = 4.07 GEE23 pKa = 4.72 GGVINVMALTAADD36 pKa = 4.06 PFQEE40 pKa = 4.34 PEE42 pKa = 4.13 SAVEE46 pKa = 3.9 AVQDD50 pKa = 3.78 HH51 pKa = 6.77 LDD53 pKa = 3.45 GRR55 pKa = 11.84 GVVILIYY62 pKa = 10.19 VPCIQAA68 pKa = 3.48
Molecular weight: 7.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.91
IPC_protein 3.757
Toseland 3.592
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.804
Protein with the highest isoelectric point:
>tr|A0A2V1JYB7|A0A2V1JYB7_9BURK Flg_hook domain-containing protein OS=Corticimicrobacter populi OX=2175229 GN=DD235_15245 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 8.99 TRR25 pKa = 11.84 AGRR28 pKa = 11.84 AVLSARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.92 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3237
0
3237
1076608
34
3991
332.6
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.944 ± 0.054
0.923 ± 0.014
5.524 ± 0.039
5.268 ± 0.042
3.38 ± 0.027
8.073 ± 0.046
2.354 ± 0.024
4.943 ± 0.036
2.563 ± 0.032
11.231 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.474 ± 0.024
2.636 ± 0.03
5.025 ± 0.036
4.611 ± 0.038
7.129 ± 0.042
5.579 ± 0.031
5.217 ± 0.03
7.19 ± 0.037
1.522 ± 0.021
2.416 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here