Gordonia phage Emperor

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q4B7|A0A2Z4Q4B7_9CAUD Integrase OS=Gordonia phage Emperor OX=2201427 GN=19 PE=3 SV=1
MM1 pKa = 7.28IVAALIVAAIALAVATAALILAGLAIAAAAALDD34 pKa = 3.87DD35 pKa = 5.95LIPNPLDD42 pKa = 3.63AQEE45 pKa = 4.49STDD48 pKa = 3.47HH49 pKa = 7.34DD50 pKa = 3.81RR51 pKa = 11.84TT52 pKa = 3.84

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q4D2|A0A2Z4Q4D2_9CAUD Terminase large subunit OS=Gordonia phage Emperor OX=2201427 GN=2 PE=4 SV=1
MM1 pKa = 7.92ADD3 pKa = 2.99AQVRR7 pKa = 11.84VEE9 pKa = 4.07GARR12 pKa = 11.84EE13 pKa = 3.81LRR15 pKa = 11.84RR16 pKa = 11.84SMRR19 pKa = 11.84QAEE22 pKa = 4.02IDD24 pKa = 3.59LTEE27 pKa = 4.11MKK29 pKa = 7.93EE30 pKa = 3.83THH32 pKa = 6.4ARR34 pKa = 11.84VASLVAGRR42 pKa = 11.84GRR44 pKa = 11.84SAAPVASGRR53 pKa = 11.84LAGTVRR59 pKa = 11.84AGATRR64 pKa = 11.84TAAVVRR70 pKa = 11.84AGRR73 pKa = 11.84KK74 pKa = 7.75SVPYY78 pKa = 9.88AGPIHH83 pKa = 6.58WGWPARR89 pKa = 11.84GITANPWLAGAAAEE103 pKa = 5.6SEE105 pKa = 4.49PAWLALYY112 pKa = 9.69TDD114 pKa = 4.64AVDD117 pKa = 4.15QIIDD121 pKa = 4.21RR122 pKa = 11.84IEE124 pKa = 4.31GTHH127 pKa = 5.45TPP129 pKa = 3.73

Molecular weight: 13.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

5031

33

619

209.6

22.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.206 ± 1.02

0.537 ± 0.142

6.241 ± 0.406

4.651 ± 0.261

2.345 ± 0.229

9.581 ± 1.036

2.067 ± 0.245

4.611 ± 0.373

2.127 ± 0.371

7.871 ± 0.457

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.63 ± 0.154

2.326 ± 0.243

6.758 ± 0.562

2.922 ± 0.229

7.275 ± 0.524

4.751 ± 0.361

7.792 ± 0.438

7.275 ± 0.413

2.067 ± 0.249

1.968 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski