Microvirus sp.
Average proteome isoelectric point is 7.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4GUJ1|A0A2Z4GUJ1_9VIRU Uncharacterized protein OS=Microvirus sp. OX=2202559 PE=4 SV=1
MM1 pKa = 7.25 FANEE5 pKa = 4.29 FLTYY9 pKa = 9.99 IPPIGEE15 pKa = 4.48 DD16 pKa = 3.4 LQHH19 pKa = 6.67 TIVSDD24 pKa = 3.96 DD25 pKa = 3.56 CTKK28 pKa = 10.67 LHH30 pKa = 6.6 SDD32 pKa = 3.78 IYY34 pKa = 9.74 LLHH37 pKa = 7.23 HH38 pKa = 6.59 IGEE41 pKa = 4.86 LNVSNNLADD50 pKa = 5.62 AITSRR55 pKa = 11.84 LQSVSDD61 pKa = 3.94 SMPDD65 pKa = 3.56 DD66 pKa = 4.01 LRR68 pKa = 11.84 QSFDD72 pKa = 3.54 KK73 pKa = 11.29 LDD75 pKa = 4.09 DD76 pKa = 3.91 FEE78 pKa = 6.35 KK79 pKa = 10.71 MEE81 pKa = 4.39 VTDD84 pKa = 3.37 SRR86 pKa = 11.84 YY87 pKa = 10.19 AQWVSDD93 pKa = 3.43 KK94 pKa = 11.01 VSRR97 pKa = 11.84 TKK99 pKa = 10.93 QFMKK103 pKa = 10.77 DD104 pKa = 2.7 FDD106 pKa = 4.25 TAVSKK111 pKa = 11.29 LKK113 pKa = 9.75 DD114 pKa = 3.46 TEE116 pKa = 4.17 EE117 pKa = 4.13 SQKK120 pKa = 11.14 LKK122 pKa = 10.62 AAQKK126 pKa = 10.01 NLRR129 pKa = 11.84 DD130 pKa = 4.34 FILRR134 pKa = 11.84 LGSDD138 pKa = 4.87 DD139 pKa = 5.21 IPDD142 pKa = 3.69 KK143 pKa = 10.91 EE144 pKa = 4.09
Molecular weight: 16.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.734
IPC2_protein 4.647
IPC_protein 4.596
Toseland 4.418
ProMoST 4.685
Dawson 4.584
Bjellqvist 4.774
Wikipedia 4.52
Rodwell 4.444
Grimsley 4.329
Solomon 4.584
Lehninger 4.546
Nozaki 4.698
DTASelect 4.952
Thurlkill 4.457
EMBOSS 4.533
Sillero 4.736
Patrickios 4.228
IPC_peptide 4.584
IPC2_peptide 4.724
IPC2.peptide.svr19 4.667
Protein with the highest isoelectric point:
>tr|A0A2Z4GUP5|A0A2Z4GUP5_9VIRU Uncharacterized protein OS=Microvirus sp. OX=2202559 PE=4 SV=1
MM1 pKa = 7.39 LLSFKK6 pKa = 10.37 IHH8 pKa = 5.96 LQVLSLLITIKK19 pKa = 10.28 WLVLLMFNVRR29 pKa = 11.84 LNKK32 pKa = 9.98 PILIGYY38 pKa = 7.44 LAKK41 pKa = 10.61 SKK43 pKa = 10.77 LVLLLLYY50 pKa = 10.51 RR51 pKa = 11.84 MYY53 pKa = 11.04 VPWFLRR59 pKa = 11.84 CILIMLKK66 pKa = 10.62 LKK68 pKa = 9.55 TLMLWLRR75 pKa = 11.84 LIMLKK80 pKa = 10.57 KK81 pKa = 10.1 LVCVLIISCKK91 pKa = 9.75 ILFLICLLVWLKK103 pKa = 10.71 ISILKK108 pKa = 10.45 VMLNKK113 pKa = 10.01 TNGVVVLTFLYY124 pKa = 10.97 LSMLLPLLLRR134 pKa = 11.84 SLHH137 pKa = 6.44 LLLNLVGKK145 pKa = 9.68 QKK147 pKa = 10.94 NIRR150 pKa = 11.84 NNNIIFYY157 pKa = 8.43 YY158 pKa = 10.14 TLLL161 pKa = 4.91
Molecular weight: 19.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.284
IPC2_protein 10.101
IPC_protein 10.379
Toseland 10.76
ProMoST 10.321
Dawson 10.862
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.477
Grimsley 10.906
Solomon 10.906
Lehninger 10.877
Nozaki 10.745
DTASelect 10.496
Thurlkill 10.745
EMBOSS 11.14
Sillero 10.774
Patrickios 11.199
IPC_peptide 10.906
IPC2_peptide 9.414
IPC2.peptide.svr19 8.311
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1922
102
640
320.3
36.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.66 ± 1.794
1.925 ± 0.589
5.671 ± 0.864
3.954 ± 0.791
5.463 ± 0.919
4.318 ± 0.843
2.393 ± 0.601
5.307 ± 0.74
5.723 ± 0.765
10.822 ± 2.407
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.341 ± 0.515
5.931 ± 0.911
3.85 ± 0.442
4.527 ± 1.205
5.047 ± 0.627
9.781 ± 0.761
4.475 ± 0.43
5.151 ± 0.594
1.301 ± 0.264
5.359 ± 0.921
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here