Infectious pancreatic necrosis virus (strain Sp) (IPNV)
Average proteome isoelectric point is 5.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q703G8|Q703G8_IPNVS Isoform of P22174 Protein VP1 OS=Infectious pancreatic necrosis virus (strain Sp) OX=11005 GN=VP1 PE=4 SV=1
MM1 pKa = 6.89 NTNKK5 pKa = 8.97 ATATYY10 pKa = 9.13 LKK12 pKa = 10.5 SIMLPEE18 pKa = 4.74 TGPASIPDD26 pKa = 5.16 DD27 pKa = 3.12 ITEE30 pKa = 3.99 RR31 pKa = 11.84 HH32 pKa = 5.89 ILKK35 pKa = 10.07 QEE37 pKa = 3.84 TSSYY41 pKa = 9.6 NLEE44 pKa = 4.05 VSEE47 pKa = 4.58 SGSGVLVCFPGAPGSRR63 pKa = 11.84 IGAHH67 pKa = 5.45 YY68 pKa = 9.97 RR69 pKa = 11.84 WNANQTGLEE78 pKa = 3.87 FDD80 pKa = 3.19 QWLEE84 pKa = 3.86 TSQDD88 pKa = 3.07 LKK90 pKa = 11.21 KK91 pKa = 10.75 AFNYY95 pKa = 9.81 GRR97 pKa = 11.84 LISRR101 pKa = 11.84 KK102 pKa = 9.31 YY103 pKa = 10.36 DD104 pKa = 3.38 IQSSTLPAGLYY115 pKa = 10.34 ALNGTLNAATFEE127 pKa = 4.46 GSLSEE132 pKa = 4.62 VEE134 pKa = 4.23 SLTYY138 pKa = 10.53 NSLMSLTTNPQDD150 pKa = 3.31 KK151 pKa = 10.9 VNNQLVTKK159 pKa = 10.21 GVTVLNLPTGFDD171 pKa = 3.17 KK172 pKa = 10.97 PYY174 pKa = 10.8 VRR176 pKa = 11.84 LEE178 pKa = 4.22 DD179 pKa = 3.65 EE180 pKa = 4.74 TPQGLQSMNGAKK192 pKa = 8.9 MRR194 pKa = 11.84 CTAAIAPRR202 pKa = 11.84 RR203 pKa = 11.84 YY204 pKa = 10.24 EE205 pKa = 3.63 IDD207 pKa = 3.84 LPSQRR212 pKa = 11.84 LPPVPATGTLTTLYY226 pKa = 9.76 EE227 pKa = 4.36 GNADD231 pKa = 3.14 IVNSTTVTGDD241 pKa = 3.01 INFSLAEE248 pKa = 3.99 QPADD252 pKa = 3.32 EE253 pKa = 4.7 TKK255 pKa = 10.68 FDD257 pKa = 3.87 FQLDD261 pKa = 3.72 FMGLDD266 pKa = 3.12 NDD268 pKa = 4.11 VPVVTVVSSVLATNDD283 pKa = 2.83 NYY285 pKa = 11.11 RR286 pKa = 11.84 GVSAKK291 pKa = 7.47 MTQSIPTEE299 pKa = 4.45 NITKK303 pKa = 10.03 PITRR307 pKa = 11.84 VKK309 pKa = 10.53 LSYY312 pKa = 10.45 KK313 pKa = 10.06 INQQTAIGNVATLGTMGPASVSFSSGNGNVPGVLRR348 pKa = 11.84 PITLVAYY355 pKa = 9.88 EE356 pKa = 4.56 KK357 pKa = 7.77 MTPLSILTVAGVSNYY372 pKa = 9.4 EE373 pKa = 4.44 LIPNPEE379 pKa = 4.27 LLKK382 pKa = 11.42 NMVTRR387 pKa = 11.84 YY388 pKa = 9.74 GKK390 pKa = 10.45 YY391 pKa = 10.22 DD392 pKa = 3.71 PEE394 pKa = 4.29 GLNYY398 pKa = 10.95 AKK400 pKa = 10.06 MILSHH405 pKa = 7.46 RR406 pKa = 11.84 EE407 pKa = 3.56 EE408 pKa = 4.78 LDD410 pKa = 2.89 IRR412 pKa = 11.84 TVWRR416 pKa = 11.84 TEE418 pKa = 3.79 EE419 pKa = 3.98 YY420 pKa = 10.51 KK421 pKa = 10.88 EE422 pKa = 3.75 RR423 pKa = 11.84 TRR425 pKa = 11.84 VFNEE429 pKa = 3.48 ITDD432 pKa = 4.37 FSSDD436 pKa = 3.78 LPTSKK441 pKa = 10.5 AWGWRR446 pKa = 11.84 DD447 pKa = 2.97 IVRR450 pKa = 11.84 GIRR453 pKa = 11.84 KK454 pKa = 8.54 VAAPVLSTLFPMAAPLIGMADD475 pKa = 3.49 QFIGDD480 pKa = 3.9 LTKK483 pKa = 10.75 TNAAGGRR490 pKa = 11.84 YY491 pKa = 9.37 HH492 pKa = 8.3 SMAAGGRR499 pKa = 11.84 YY500 pKa = 9.4 KK501 pKa = 10.73 DD502 pKa = 4.16 VLEE505 pKa = 4.56 SWASGGPDD513 pKa = 3.01 GKK515 pKa = 10.61 FSRR518 pKa = 11.84 ALKK521 pKa = 10.49 NRR523 pKa = 11.84 LEE525 pKa = 4.14 SANYY529 pKa = 10.01 EE530 pKa = 4.32 EE531 pKa = 5.78 VEE533 pKa = 4.58 LPPPSKK539 pKa = 10.69 GVIVPVVHH547 pKa = 5.83 TVKK550 pKa = 10.46 SAPGEE555 pKa = 4.17 AFGSLAIIIPGEE567 pKa = 4.1 YY568 pKa = 9.88 PEE570 pKa = 5.82 LLDD573 pKa = 5.53 ANQQVLSHH581 pKa = 6.79 FANDD585 pKa = 3.56 TGSVWGIGEE594 pKa = 5.12 DD595 pKa = 4.07 IPFEE599 pKa = 4.38 GDD601 pKa = 3.29 NMCYY605 pKa = 9.05 TALPLKK611 pKa = 9.86 EE612 pKa = 3.89 IKK614 pKa = 10.42 RR615 pKa = 11.84 NGNIVVEE622 pKa = 4.86 KK623 pKa = 10.34 IFAGPIMGPSAQLGLSLLVNDD644 pKa = 5.1 IEE646 pKa = 5.31 DD647 pKa = 3.96 GVPRR651 pKa = 11.84 MVFTGEE657 pKa = 3.79 IADD660 pKa = 4.34 DD661 pKa = 4.22 EE662 pKa = 4.48 EE663 pKa = 4.84 TIIPICGVDD672 pKa = 3.33 IKK674 pKa = 11.13 AIAAHH679 pKa = 5.51 EE680 pKa = 4.08 QGLPLIGNQPGVDD693 pKa = 3.08 EE694 pKa = 4.43 EE695 pKa = 4.5 VRR697 pKa = 11.84 NTSLAAHH704 pKa = 7.67 LIQTGTLPVQRR715 pKa = 11.84 AKK717 pKa = 11.05 GSNKK721 pKa = 9.2 RR722 pKa = 11.84 IKK724 pKa = 10.75 YY725 pKa = 9.78 LGEE728 pKa = 3.98 LMASNASGMDD738 pKa = 3.43 EE739 pKa = 3.92 EE740 pKa = 5.05 LQRR743 pKa = 11.84 LLNATMARR751 pKa = 11.84 AKK753 pKa = 9.75 EE754 pKa = 4.04 VQDD757 pKa = 3.54 AEE759 pKa = 4.25 IYY761 pKa = 10.62 KK762 pKa = 10.24 LLKK765 pKa = 10.5 LMAWTRR771 pKa = 11.84 KK772 pKa = 9.59 NDD774 pKa = 3.94 LTDD777 pKa = 5.05 HH778 pKa = 5.96 MYY780 pKa = 10.29 EE781 pKa = 3.96 WSKK784 pKa = 11.34 EE785 pKa = 4.01 DD786 pKa = 3.59 PDD788 pKa = 3.72 ALKK791 pKa = 10.49 FGKK794 pKa = 10.38 LISTPPKK801 pKa = 10.27 HH802 pKa = 6.15 PEE804 pKa = 3.87 KK805 pKa = 10.57 PKK807 pKa = 11.16 GPDD810 pKa = 2.87 QHH812 pKa = 6.6 HH813 pKa = 6.05 AQEE816 pKa = 4.23 ARR818 pKa = 11.84 ATRR821 pKa = 11.84 ISLDD825 pKa = 3.32 AVRR828 pKa = 11.84 AGADD832 pKa = 3.26 FATPEE837 pKa = 3.85 WVALNNYY844 pKa = 8.8 RR845 pKa = 11.84 GPSPGQFKK853 pKa = 11.0 YY854 pKa = 11.19 YY855 pKa = 10.24 LITGRR860 pKa = 11.84 EE861 pKa = 4.09 PEE863 pKa = 4.93 PGDD866 pKa = 3.71 EE867 pKa = 4.18 YY868 pKa = 11.25 EE869 pKa = 5.85 DD870 pKa = 4.6 YY871 pKa = 10.8 IKK873 pKa = 10.78 QPIVKK878 pKa = 8.42 PTDD881 pKa = 3.21 MNKK884 pKa = 9.11 IRR886 pKa = 11.84 RR887 pKa = 11.84 LANSVYY893 pKa = 10.06 GLPHH897 pKa = 6.09 QEE899 pKa = 3.77 PAPEE903 pKa = 3.88 EE904 pKa = 4.51 FYY906 pKa = 11.33 DD907 pKa = 3.79 AVAAVFAQNGGRR919 pKa = 11.84 GPDD922 pKa = 3.35 QDD924 pKa = 3.31 QMQDD928 pKa = 3.13 LRR930 pKa = 11.84 EE931 pKa = 4.26 LARR934 pKa = 11.84 QMKK937 pKa = 9.58 RR938 pKa = 11.84 RR939 pKa = 11.84 PRR941 pKa = 11.84 NADD944 pKa = 2.72 APRR947 pKa = 11.84 RR948 pKa = 11.84 TRR950 pKa = 11.84 APAEE954 pKa = 3.76 PAPPGRR960 pKa = 11.84 SRR962 pKa = 11.84 FTPSGDD968 pKa = 3.33 NAEE971 pKa = 4.26 VV972 pKa = 3.07
Molecular weight: 106.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.295
IPC2_protein 5.334
IPC_protein 5.308
Toseland 5.347
ProMoST 5.486
Dawson 5.347
Bjellqvist 5.448
Wikipedia 5.232
Rodwell 5.27
Grimsley 5.296
Solomon 5.347
Lehninger 5.308
Nozaki 5.486
DTASelect 5.639
Thurlkill 5.359
EMBOSS 5.321
Sillero 5.563
Patrickios 4.279
IPC_peptide 5.359
IPC2_peptide 5.563
IPC2.peptide.svr19 5.552
Protein with the highest isoelectric point:
>sp|Q703G9|POLS_IPNVS Structural polyprotein OS=Infectious pancreatic necrosis virus (strain Sp) OX=11005 PE=1 SV=1
MM1 pKa = 7.67 SDD3 pKa = 4.08 IFNSPQNKK11 pKa = 9.77 ASILNALMKK20 pKa = 9.7 STQGDD25 pKa = 3.83 VEE27 pKa = 4.64 DD28 pKa = 3.71 VLIPKK33 pKa = 10.06 RR34 pKa = 11.84 FRR36 pKa = 11.84 PAKK39 pKa = 10.35 DD40 pKa = 3.61 PLDD43 pKa = 4.22 SPQAAAAFLKK53 pKa = 8.53 EE54 pKa = 3.81 HH55 pKa = 7.62 KK56 pKa = 10.37 YY57 pKa = 10.63 RR58 pKa = 11.84 ILRR61 pKa = 11.84 PRR63 pKa = 11.84 AIPTMVEE70 pKa = 3.76 IEE72 pKa = 3.98 TDD74 pKa = 3.16 AALPRR79 pKa = 11.84 LAAMVDD85 pKa = 3.51 DD86 pKa = 5.06 GKK88 pKa = 11.05 LKK90 pKa = 10.86 EE91 pKa = 4.23 MVNVPEE97 pKa = 4.55 GTTAFYY103 pKa = 10.05 PKK105 pKa = 9.82 YY106 pKa = 10.15 YY107 pKa = 9.64 PFHH110 pKa = 7.17 RR111 pKa = 11.84 PDD113 pKa = 4.02 HH114 pKa = 7.24 DD115 pKa = 4.14 DD116 pKa = 3.18 VGTFGAPDD124 pKa = 3.15 ITLLKK129 pKa = 10.52 QLTFFLLEE137 pKa = 3.99 NDD139 pKa = 4.02 FPTGPEE145 pKa = 3.55 TLRR148 pKa = 11.84 QVRR151 pKa = 11.84 EE152 pKa = 4.48 AIATLQYY159 pKa = 11.35 GSGSYY164 pKa = 10.01 SGQLNRR170 pKa = 11.84 LLAMKK175 pKa = 10.44 GVATGRR181 pKa = 11.84 NPNKK185 pKa = 9.57 TPLAVGYY192 pKa = 8.5 TNEE195 pKa = 3.64 QMARR199 pKa = 11.84 LMEE202 pKa = 4.05 QTLPINPPKK211 pKa = 10.96 NEE213 pKa = 4.26 DD214 pKa = 3.58 PDD216 pKa = 4.95 LRR218 pKa = 11.84 WAPSWLIQYY227 pKa = 8.7 TGDD230 pKa = 3.31 ASTDD234 pKa = 3.1 KK235 pKa = 11.06 SYY237 pKa = 11.24 LPHH240 pKa = 5.43 VTAKK244 pKa = 10.48 SSAGLPYY251 pKa = 9.88 IGKK254 pKa = 8.15 TKK256 pKa = 10.95 GDD258 pKa = 3.64 TTAEE262 pKa = 4.03 ALVLADD268 pKa = 4.42 SFIRR272 pKa = 11.84 DD273 pKa = 3.54 LGKK276 pKa = 10.31 AATSADD282 pKa = 3.33 PGAAAKK288 pKa = 10.14 KK289 pKa = 9.51 VLSDD293 pKa = 3.77 FWYY296 pKa = 10.37 LSCGLLFPKK305 pKa = 10.51 GEE307 pKa = 4.59 RR308 pKa = 11.84 YY309 pKa = 7.04 TQKK312 pKa = 11.01 DD313 pKa = 2.99 WDD315 pKa = 4.02 LKK317 pKa = 9.48 TRR319 pKa = 11.84 NIWSAPYY326 pKa = 7.69 PTHH329 pKa = 7.06 LLLSMVSSPVMDD341 pKa = 3.73 EE342 pKa = 4.1 SKK344 pKa = 11.34 LNITNTQTPSLYY356 pKa = 10.6 GFSPFHH362 pKa = 6.53 GGINRR367 pKa = 11.84 IMTIIRR373 pKa = 11.84 EE374 pKa = 4.27 HH375 pKa = 7.06 LDD377 pKa = 3.36 QEE379 pKa = 4.18 QDD381 pKa = 3.18 LVMIYY386 pKa = 10.95 ADD388 pKa = 4.6 NIYY391 pKa = 10.23 ILQDD395 pKa = 3.45 NTWYY399 pKa = 10.96 SIDD402 pKa = 4.01 LEE404 pKa = 4.4 KK405 pKa = 11.54 GEE407 pKa = 4.74 ANCTPQHH414 pKa = 5.47 MQAMMYY420 pKa = 10.23 YY421 pKa = 9.91 RR422 pKa = 11.84 LTRR425 pKa = 11.84 EE426 pKa = 3.81 WTNEE430 pKa = 3.98 DD431 pKa = 2.94 GSPRR435 pKa = 11.84 YY436 pKa = 9.89 NPTWATFAMYY446 pKa = 9.92 VGPSMVVDD454 pKa = 4.21 STCLLMNLQLKK465 pKa = 6.82 TTGQGSGNAFTFLNNHH481 pKa = 6.13 LMSTIVVAEE490 pKa = 3.65 WHH492 pKa = 6.25 KK493 pKa = 11.05 AGRR496 pKa = 11.84 PNPMSKK502 pKa = 10.45 EE503 pKa = 3.95 FMDD506 pKa = 5.28 LEE508 pKa = 4.64 AKK510 pKa = 9.88 TGINFKK516 pKa = 10.2 IEE518 pKa = 4.2 RR519 pKa = 11.84 EE520 pKa = 4.18 LKK522 pKa = 9.94 DD523 pKa = 3.35 LRR525 pKa = 11.84 SIIMEE530 pKa = 4.43 AVDD533 pKa = 3.58 TAPLDD538 pKa = 4.01 GYY540 pKa = 11.04 LADD543 pKa = 5.56 GSDD546 pKa = 3.95 LPPRR550 pKa = 11.84 VPGKK554 pKa = 10.37 AVEE557 pKa = 4.12 LDD559 pKa = 3.63 LLGWSAVYY567 pKa = 10.23 SRR569 pKa = 11.84 QLEE572 pKa = 4.22 MFVPVLEE579 pKa = 4.05 NEE581 pKa = 4.02 RR582 pKa = 11.84 LIASVAYY589 pKa = 9.32 PKK591 pKa = 10.89 GLEE594 pKa = 4.09 NKK596 pKa = 10.05 SLARR600 pKa = 11.84 KK601 pKa = 8.88 PGAEE605 pKa = 3.3 IAYY608 pKa = 9.8 QIVRR612 pKa = 11.84 YY613 pKa = 8.57 EE614 pKa = 4.38 AIRR617 pKa = 11.84 LIGGWNNPLIEE628 pKa = 4.13 TAAKK632 pKa = 10.34 HH633 pKa = 5.12 MSLDD637 pKa = 3.04 KK638 pKa = 10.75 RR639 pKa = 11.84 KK640 pKa = 8.93 RR641 pKa = 11.84 LEE643 pKa = 3.9 VKK645 pKa = 10.72 GIDD648 pKa = 3.35 VTGFLDD654 pKa = 3.56 DD655 pKa = 3.97 WNTMSEE661 pKa = 4.18 FGGDD665 pKa = 3.55 LEE667 pKa = 5.43 GISLTAPLTNQTLLDD682 pKa = 3.63 INTPEE687 pKa = 4.05 TEE689 pKa = 4.02 FDD691 pKa = 3.79 VKK693 pKa = 10.77 DD694 pKa = 3.84 RR695 pKa = 11.84 PPTPRR700 pKa = 11.84 SPGKK704 pKa = 8.25 TLAEE708 pKa = 3.92 VTAAITSGTYY718 pKa = 9.95 KK719 pKa = 10.54 DD720 pKa = 3.78 PKK722 pKa = 8.57 SAVWRR727 pKa = 11.84 LLDD730 pKa = 3.27 QRR732 pKa = 11.84 TKK734 pKa = 11.18 LRR736 pKa = 11.84 VSTLRR741 pKa = 11.84 DD742 pKa = 3.04 HH743 pKa = 7.15 AHH745 pKa = 6.38 ALKK748 pKa = 9.97 PAASTSDD755 pKa = 3.35 FWGDD759 pKa = 3.15 ATEE762 pKa = 4.83 EE763 pKa = 4.01 LAEE766 pKa = 3.99 QQQLLMKK773 pKa = 10.59 ANNLLKK779 pKa = 10.81 SSLTEE784 pKa = 3.57 ARR786 pKa = 11.84 EE787 pKa = 3.98 ALEE790 pKa = 4.35 TVQSDD795 pKa = 3.89 KK796 pKa = 10.91 IISGKK801 pKa = 8.33 TSPEE805 pKa = 4.1 KK806 pKa = 11.11 NPGTAANPVVAYY818 pKa = 10.43 GEE820 pKa = 4.16 FSEE823 pKa = 5.61 KK824 pKa = 10.33 IPLTPTQKK832 pKa = 10.69 KK833 pKa = 7.32 NAKK836 pKa = 9.43 RR837 pKa = 11.84 RR838 pKa = 11.84 EE839 pKa = 3.99 KK840 pKa = 10.41 QRR842 pKa = 11.84 RR843 pKa = 11.84 NN844 pKa = 3.14
Molecular weight: 94.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.946
IPC2_protein 5.893
IPC_protein 5.957
Toseland 6.364
ProMoST 6.275
Dawson 6.186
Bjellqvist 6.198
Wikipedia 6.186
Rodwell 6.173
Grimsley 6.547
Solomon 6.186
Lehninger 6.173
Nozaki 6.44
DTASelect 6.634
Thurlkill 6.634
EMBOSS 6.59
Sillero 6.547
Patrickios 4.469
IPC_peptide 6.198
IPC2_peptide 6.453
IPC2.peptide.svr19 6.569
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
1
3
2660
844
972
886.7
98.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.571 ± 0.119
0.376 ± 0.015
6.053 ± 0.22
6.316 ± 0.204
2.857 ± 0.078
6.617 ± 0.47
1.466 ± 0.055
5.0 ± 0.195
6.053 ± 0.396
9.812 ± 0.509
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.895 ± 0.139
4.85 ± 0.221
7.03 ± 0.118
3.534 ± 0.037
5.113 ± 0.123
6.203 ± 0.032
7.368 ± 0.249
5.15 ± 0.392
1.429 ± 0.172
3.308 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here