Azospirillum sp. CAG:239

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Azospirillum; environmental samples

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1791 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6J779|R6J779_9PROT Superoxide dismutase OS=Azospirillum sp. CAG:239 OX=1262705 GN=BN554_00310 PE=3 SV=1
MM1 pKa = 7.52INIYY5 pKa = 10.38NNQGTIQDD13 pKa = 4.75AISKK17 pKa = 9.81VGGVSYY23 pKa = 10.84LDD25 pKa = 3.46CCIWSSSEE33 pKa = 4.48IDD35 pKa = 6.04DD36 pKa = 3.59FDD38 pKa = 3.22AWYY41 pKa = 10.94SRR43 pKa = 11.84LDD45 pKa = 3.43EE46 pKa = 5.56SYY48 pKa = 11.7GLDD51 pKa = 3.56NDD53 pKa = 4.58SKK55 pKa = 11.46SLDD58 pKa = 3.77NYY60 pKa = 7.82VHH62 pKa = 6.9PVLEE66 pKa = 4.55FF67 pKa = 4.01

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6IVX9|R6IVX9_9PROT Uncharacterized protein OS=Azospirillum sp. CAG:239 OX=1262705 GN=BN554_00120 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.07VIAARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.77NLSAA44 pKa = 4.73

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1791

0

1791

522069

30

2217

291.5

32.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.667 ± 0.063

1.312 ± 0.039

5.658 ± 0.043

6.903 ± 0.065

4.54 ± 0.053

6.943 ± 0.066

1.528 ± 0.025

6.613 ± 0.053

7.021 ± 0.062

9.361 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.033

4.827 ± 0.051

3.74 ± 0.037

3.096 ± 0.033

5.346 ± 0.044

5.817 ± 0.049

4.694 ± 0.047

6.827 ± 0.057

0.993 ± 0.022

3.538 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski