Sewage-associated gemycircularvirus 9

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus sewopo5; Sewage derived gemycircularvirus 5

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7CL54|A0A0A7CL54_9VIRU Capsid protein OS=Sewage-associated gemycircularvirus 9 OX=1519404 PE=4 SV=1
MM1 pKa = 7.91PFNLHH6 pKa = 6.06CRR8 pKa = 11.84YY9 pKa = 10.45ALLTYY14 pKa = 8.89AQCGDD19 pKa = 4.5LSPTAVGEE27 pKa = 4.16FFDD30 pKa = 3.66NLGYY34 pKa = 10.65KK35 pKa = 9.9SVIGRR40 pKa = 11.84EE41 pKa = 3.77NHH43 pKa = 6.65ADD45 pKa = 3.51GGVHH49 pKa = 5.94LHH51 pKa = 6.49CFVDD55 pKa = 4.74FGRR58 pKa = 11.84KK59 pKa = 7.94RR60 pKa = 11.84RR61 pKa = 11.84FRR63 pKa = 11.84RR64 pKa = 11.84ARR66 pKa = 11.84VFDD69 pKa = 3.33IEE71 pKa = 4.14NRR73 pKa = 11.84HH74 pKa = 6.13PNVEE78 pKa = 4.11PSRR81 pKa = 11.84GTPEE85 pKa = 4.4KK86 pKa = 10.5GWDD89 pKa = 3.54YY90 pKa = 11.42AVKK93 pKa = 10.71DD94 pKa = 3.87GDD96 pKa = 3.36ICYY99 pKa = 10.27QSLDD103 pKa = 3.56RR104 pKa = 11.84PRR106 pKa = 11.84EE107 pKa = 3.95SSPSGGSNGGTRR119 pKa = 11.84DD120 pKa = 2.86KK121 pKa = 10.3WASITGASDD130 pKa = 5.15RR131 pKa = 11.84EE132 pKa = 4.38AFWDD136 pKa = 4.24LVHH139 pKa = 7.02EE140 pKa = 5.15LDD142 pKa = 4.48PKK144 pKa = 10.83SAACCFTQLQKK155 pKa = 11.05YY156 pKa = 8.98CDD158 pKa = 3.24WKK160 pKa = 10.42FAPRR164 pKa = 11.84TPEE167 pKa = 3.8YY168 pKa = 9.78EE169 pKa = 4.24SPGGLEE175 pKa = 4.46FIGGGLDD182 pKa = 5.38GRR184 pKa = 11.84DD185 pKa = 3.26DD186 pKa = 3.68WLLQSGIGGRR196 pKa = 11.84EE197 pKa = 3.73AFVGEE202 pKa = 4.26LVCRR206 pKa = 11.84RR207 pKa = 11.84CMSICVYY214 pKa = 10.83GEE216 pKa = 3.81SRR218 pKa = 11.84TGKK221 pKa = 7.35TLWARR226 pKa = 11.84SLGPHH231 pKa = 7.14IYY233 pKa = 10.33CVGLVSGDD241 pKa = 3.51EE242 pKa = 4.16CMKK245 pKa = 11.05ASTADD250 pKa = 3.43YY251 pKa = 11.18AVFDD255 pKa = 5.43DD256 pKa = 3.7IRR258 pKa = 11.84GGIKK262 pKa = 9.99FFPSFKK268 pKa = 10.33EE269 pKa = 3.83WLGCQAWVSVKK280 pKa = 10.37CLYY283 pKa = 10.51RR284 pKa = 11.84EE285 pKa = 4.02PKK287 pKa = 8.81LVKK290 pKa = 9.08WGKK293 pKa = 9.13PSIWLSNTDD302 pKa = 3.02PRR304 pKa = 11.84DD305 pKa = 3.42YY306 pKa = 10.79MEE308 pKa = 5.32NSDD311 pKa = 4.57IDD313 pKa = 3.87WMNKK317 pKa = 7.88NCIFVEE323 pKa = 4.34VNSPIFRR330 pKa = 11.84ANTEE334 pKa = 3.84

Molecular weight: 37.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7CL72|A0A0A7CL72_9VIRU RepA OS=Sewage-associated gemycircularvirus 9 OX=1519404 PE=3 SV=1
MM1 pKa = 7.28RR2 pKa = 11.84RR3 pKa = 11.84SRR5 pKa = 11.84RR6 pKa = 11.84FPAKK10 pKa = 9.97RR11 pKa = 11.84RR12 pKa = 11.84FTRR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 5.0TTYY20 pKa = 8.83RR21 pKa = 11.84RR22 pKa = 11.84PRR24 pKa = 11.84RR25 pKa = 11.84TITKK29 pKa = 8.6RR30 pKa = 11.84SYY32 pKa = 9.81RR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84PMRR39 pKa = 11.84RR40 pKa = 11.84MTRR43 pKa = 11.84KK44 pKa = 10.11SILNTTSTKK53 pKa = 10.46KK54 pKa = 10.04RR55 pKa = 11.84DD56 pKa = 3.25TMIAGNTFPALPEE69 pKa = 3.95NVGALTVTSDD79 pKa = 3.52VPAVLLWCPTARR91 pKa = 11.84QLADD95 pKa = 3.23TRR97 pKa = 11.84SALSTMPVNAQRR109 pKa = 11.84LSSTPFIVGLKK120 pKa = 7.67EE121 pKa = 4.11TITIRR126 pKa = 11.84TDD128 pKa = 2.9TSNAWRR134 pKa = 11.84WRR136 pKa = 11.84RR137 pKa = 11.84IVFFLKK143 pKa = 10.41GGPPGFTDD151 pKa = 2.87VGDD154 pKa = 3.69INRR157 pKa = 11.84VYY159 pKa = 11.13SPVNAGTDD167 pKa = 3.4FDD169 pKa = 4.46TYY171 pKa = 11.28QRR173 pKa = 11.84TATALPFALVADD185 pKa = 4.81VYY187 pKa = 11.65ASIFRR192 pKa = 11.84GFGINNVSATPQDD205 pKa = 3.27WLDD208 pKa = 4.92PITAPIDD215 pKa = 3.46TSRR218 pKa = 11.84ITPVYY223 pKa = 10.78DD224 pKa = 2.99HH225 pKa = 6.42TTSIRR230 pKa = 11.84SGNDD234 pKa = 2.58TGVVKK239 pKa = 8.26TTTSGTVSGRR249 pKa = 11.84HH250 pKa = 4.51STNNDD255 pKa = 3.07SEE257 pKa = 4.5IGGNMLSSAFSTHH270 pKa = 6.48FKK272 pKa = 10.08PGCGDD277 pKa = 3.68MYY279 pKa = 11.48VMDD282 pKa = 5.41IIIGNSAEE290 pKa = 4.32SSDD293 pKa = 3.87ALQFLPTSTLYY304 pKa = 9.65WHH306 pKa = 7.06EE307 pKa = 4.2KK308 pKa = 9.32

Molecular weight: 34.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

914

272

334

304.7

34.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.127 ± 0.276

3.063 ± 0.954

6.893 ± 0.412

4.158 ± 1.043

4.814 ± 0.256

9.519 ± 1.39

1.969 ± 0.279

4.705 ± 0.444

4.158 ± 0.412

7.002 ± 0.819

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.86 ± 0.288

3.611 ± 0.426

5.799 ± 0.314

1.969 ± 0.221

9.3 ± 0.814

7.44 ± 0.699

6.783 ± 2.546

5.252 ± 0.439

2.298 ± 0.441

3.282 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski