Garlic common latent virus
Average proteome isoelectric point is 7.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9CHA4|G9CHA4_9VIRU Movement protein TGB2 OS=Garlic common latent virus OX=47900 GN=TGB-2 PE=3 SV=1
MM1 pKa = 7.81 DD2 pKa = 3.76 VLVNKK7 pKa = 10.01 LLSFDD12 pKa = 4.12 FVRR15 pKa = 11.84 VSSKK19 pKa = 10.3 LSKK22 pKa = 10.25 PIIVNCVPGAGKK34 pKa = 9.64 SHH36 pKa = 8.22 LIRR39 pKa = 11.84 EE40 pKa = 4.63 LLRR43 pKa = 11.84 EE44 pKa = 4.07 DD45 pKa = 3.84 EE46 pKa = 4.63 RR47 pKa = 11.84 FVAYY51 pKa = 9.31 TFGADD56 pKa = 2.91 ITEE59 pKa = 4.31 TTDD62 pKa = 3.65 CITIQRR68 pKa = 11.84 VPEE71 pKa = 4.18 AKK73 pKa = 9.7 PIHH76 pKa = 6.05 NFIIIDD82 pKa = 4.28 EE83 pKa = 4.36 YY84 pKa = 11.66 QLGDD88 pKa = 3.21 WRR90 pKa = 11.84 SFEE93 pKa = 4.83 PIAIFGDD100 pKa = 3.82 PLQGSSEE107 pKa = 4.22 CLRR110 pKa = 11.84 PHH112 pKa = 6.65 FTTDD116 pKa = 2.69 LTKK119 pKa = 10.61 RR120 pKa = 11.84 FGSSTCSLLQSIGYY134 pKa = 7.79 QIRR137 pKa = 11.84 SEE139 pKa = 4.06 RR140 pKa = 11.84 TDD142 pKa = 2.64 ICIIAEE148 pKa = 4.17 VTSAEE153 pKa = 4.12 LEE155 pKa = 4.38 GVVIAYY161 pKa = 9.76 GPEE164 pKa = 4.08 AEE166 pKa = 5.38 WLLKK170 pKa = 9.95 WYY172 pKa = 9.81 GVSHH176 pKa = 6.73 LRR178 pKa = 11.84 VCEE181 pKa = 4.03 IQGKK185 pKa = 7.07 TFDD188 pKa = 3.81 VVTLVTDD195 pKa = 3.52 YY196 pKa = 11.58 SIVTEE201 pKa = 4.2 DD202 pKa = 3.45 NRR204 pKa = 11.84 RR205 pKa = 11.84 DD206 pKa = 3.39 LYY208 pKa = 11.45 LCLTRR213 pKa = 11.84 HH214 pKa = 5.61 RR215 pKa = 11.84 NKK217 pKa = 10.73 LQVLNGDD224 pKa = 3.56 ATLATSS230 pKa = 3.8
Molecular weight: 25.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.119
IPC2_protein 5.219
IPC_protein 5.143
Toseland 5.13
ProMoST 5.308
Dawson 5.169
Bjellqvist 5.283
Wikipedia 5.067
Rodwell 5.092
Grimsley 5.08
Solomon 5.169
Lehninger 5.13
Nozaki 5.308
DTASelect 5.474
Thurlkill 5.169
EMBOSS 5.13
Sillero 5.385
Patrickios 3.923
IPC_peptide 5.181
IPC2_peptide 5.385
IPC2.peptide.svr19 5.363
Protein with the highest isoelectric point:
>tr|G9CHA7|G9CHA7_9VIRU Nucleic acid binding protein OS=Garlic common latent virus OX=47900 PE=4 SV=1
MM1 pKa = 8.13 DD2 pKa = 4.64 PTTQISVSLLLVVKK16 pKa = 10.88 NKK18 pKa = 10.39 LCDD21 pKa = 3.43 SGVPTDD27 pKa = 3.58 VAIGIIEE34 pKa = 4.44 PIIKK38 pKa = 9.78 EE39 pKa = 3.89 VRR41 pKa = 11.84 KK42 pKa = 9.13 LQRR45 pKa = 11.84 QEE47 pKa = 3.69 EE48 pKa = 4.17 QRR50 pKa = 11.84 LLRR53 pKa = 11.84 FNGCSRR59 pKa = 11.84 SAIKK63 pKa = 10.36 RR64 pKa = 11.84 RR65 pKa = 11.84 AKK67 pKa = 10.42 YY68 pKa = 10.34 LNKK71 pKa = 9.82 CHH73 pKa = 6.92 KK74 pKa = 10.0 CGKK77 pKa = 8.32 QSHH80 pKa = 6.44 YY81 pKa = 10.73 GLCSRR86 pKa = 11.84 NQTISNMEE94 pKa = 3.76 VEE96 pKa = 4.22 FLIRR100 pKa = 11.84 CGTIRR105 pKa = 11.84 YY106 pKa = 7.6 LTEE109 pKa = 3.65 NPQRR113 pKa = 11.84 RR114 pKa = 11.84 KK115 pKa = 10.77 DD116 pKa = 3.64 SIYY119 pKa = 10.66 SSDD122 pKa = 3.68 YY123 pKa = 9.67 EE124 pKa = 4.28 KK125 pKa = 10.88 LVEE128 pKa = 4.05 RR129 pKa = 11.84 ATRR132 pKa = 11.84 VV133 pKa = 3.05
Molecular weight: 15.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.192
IPC2_protein 8.96
IPC_protein 8.931
Toseland 9.633
ProMoST 9.399
Dawson 9.882
Bjellqvist 9.663
Wikipedia 10.043
Rodwell 10.204
Grimsley 9.94
Solomon 9.94
Lehninger 9.911
Nozaki 9.867
DTASelect 9.589
Thurlkill 9.75
EMBOSS 10.072
Sillero 9.867
Patrickios 7.585
IPC_peptide 9.94
IPC2_peptide 8.639
IPC2.peptide.svr19 7.879
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2809
68
1951
468.2
52.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.44 ± 1.387
3.133 ± 0.44
5.055 ± 0.556
6.408 ± 0.72
5.838 ± 1.285
6.159 ± 0.407
2.421 ± 0.442
5.269 ± 1.144
5.269 ± 0.475
9.932 ± 0.966
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.851 ± 0.287
3.489 ± 0.633
4.058 ± 0.474
3.133 ± 0.451
6.515 ± 0.776
7.227 ± 0.59
6.016 ± 0.928
6.764 ± 0.337
0.926 ± 0.189
3.097 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here