Streptococcus satellite phage Javan195

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZHW6|A0A4D5ZHW6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan195 OX=2558560 GN=JavanS195_0013 PE=4 SV=1
MM1 pKa = 7.39LVTYY5 pKa = 8.27PALFYY10 pKa = 11.35YY11 pKa = 10.68DD12 pKa = 4.04DD13 pKa = 4.01TDD15 pKa = 3.86GANAPYY21 pKa = 10.03FVTFPDD27 pKa = 4.21FEE29 pKa = 4.62YY30 pKa = 10.92SATQGEE36 pKa = 4.55DD37 pKa = 3.0MADD40 pKa = 3.52AMAMASDD47 pKa = 3.34WLGIHH52 pKa = 6.56LADD55 pKa = 4.36YY56 pKa = 10.61IEE58 pKa = 4.46NGRR61 pKa = 11.84DD62 pKa = 3.05IPTPTSINALSLADD76 pKa = 3.93NNPFRR81 pKa = 11.84DD82 pKa = 3.87DD83 pKa = 3.58KK84 pKa = 11.4DD85 pKa = 3.43IEE87 pKa = 4.32LVYY90 pKa = 10.75DD91 pKa = 3.98PSKK94 pKa = 11.15SFVSMVMVDD103 pKa = 3.2VAEE106 pKa = 4.14YY107 pKa = 10.49LGSQEE112 pKa = 4.15PVKK115 pKa = 10.18KK116 pKa = 9.12TLTIPRR122 pKa = 11.84WADD125 pKa = 3.13TLGRR129 pKa = 11.84EE130 pKa = 4.01LGLNFSQTLTDD141 pKa = 5.45AIADD145 pKa = 4.03KK146 pKa = 10.56KK147 pKa = 10.31IHH149 pKa = 5.92AA150 pKa = 5.0

Molecular weight: 16.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZFJ9|A0A4D5ZFJ9_9VIRU Integrase OS=Streptococcus satellite phage Javan195 OX=2558560 GN=JavanS195_0019 PE=3 SV=1
MM1 pKa = 7.28AGKK4 pKa = 10.07RR5 pKa = 11.84LKK7 pKa = 10.8HH8 pKa = 5.98PALKK12 pKa = 10.34GLRR15 pKa = 11.84IGQKK19 pKa = 10.55NIIKK23 pKa = 10.15DD24 pKa = 3.7RR25 pKa = 11.84QPTIEE30 pKa = 4.32QIKK33 pKa = 8.53EE34 pKa = 3.65QQRR37 pKa = 11.84LKK39 pKa = 10.73KK40 pKa = 10.24LKK42 pKa = 10.01KK43 pKa = 9.67KK44 pKa = 9.88RR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 9.34

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

3000

44

388

157.9

18.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.3 ± 0.574

0.6 ± 0.216

5.6 ± 0.536

7.867 ± 0.684

3.733 ± 0.327

4.4 ± 0.385

1.667 ± 0.274

6.8 ± 0.292

9.433 ± 0.676

10.6 ± 0.605

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.232

5.867 ± 0.505

3.6 ± 0.317

5.467 ± 0.543

4.567 ± 0.459

5.267 ± 0.383

5.8 ± 0.253

4.667 ± 0.509

0.867 ± 0.243

4.167 ± 0.5
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski