Clostridium phage phi8074-B1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1TK31|I1TK31_9CAUD Uncharacterized protein OS=Clostridium phage phi8074-B1 OX=1147137 GN=phi8074-B1_00080 PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 4.96LAITAMQDD10 pKa = 3.21SLYY13 pKa = 11.05CLDD16 pKa = 4.22DD17 pKa = 4.06LLRR20 pKa = 11.84EE21 pKa = 4.18VPYY24 pKa = 10.85DD25 pKa = 3.7DD26 pKa = 3.69YY27 pKa = 12.09AGVVEE32 pKa = 4.93SAIEE36 pKa = 3.79LKK38 pKa = 10.86VRR40 pKa = 11.84IEE42 pKa = 4.08NEE44 pKa = 3.97LEE46 pKa = 4.01EE47 pKa = 5.42KK48 pKa = 10.85GIDD51 pKa = 3.7YY52 pKa = 11.03

Molecular weight: 5.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1TK19|I1TK19_9CAUD Uncharacterized protein OS=Clostridium phage phi8074-B1 OX=1147137 GN=phi8074-B1_00068 PE=4 SV=1
MM1 pKa = 7.44NIKK4 pKa = 10.16LLGMSTEE11 pKa = 4.03EE12 pKa = 3.9LKK14 pKa = 10.71QLRR17 pKa = 11.84GRR19 pKa = 11.84NAEE22 pKa = 3.93VLAKK26 pKa = 9.41YY27 pKa = 9.19VRR29 pKa = 11.84KK30 pKa = 9.83RR31 pKa = 11.84RR32 pKa = 11.84EE33 pKa = 4.25LDD35 pKa = 2.82WLIASRR41 pKa = 11.84EE42 pKa = 4.05LYY44 pKa = 9.56EE45 pKa = 4.54RR46 pKa = 11.84NGINCVARR54 pKa = 11.84FKK56 pKa = 11.21

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

14440

31

892

176.1

20.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.681 ± 0.294

1.378 ± 0.167

6.427 ± 0.213

8.393 ± 0.348

3.643 ± 0.158

6.191 ± 0.299

1.78 ± 0.149

7.839 ± 0.2

8.179 ± 0.301

9.162 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.164

5.713 ± 0.222

2.867 ± 0.147

3.878 ± 0.17

4.377 ± 0.222

4.972 ± 0.212

5.873 ± 0.364

6.004 ± 0.235

1.004 ± 0.088

4.993 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski