Pauljensenia hongkongensis
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1597 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D8B423|A0A1D8B423_9ACTO Dihydrodipicolinate synthase family protein OS=Pauljensenia hongkongensis OX=178339 GN=BH719_08625 PE=3 SV=1
MM1 pKa = 7.3 TDD3 pKa = 3.01 VYY5 pKa = 11.53 VEE7 pKa = 4.25 AGDD10 pKa = 4.62 LEE12 pKa = 4.39 RR13 pKa = 11.84 LHH15 pKa = 8.15 SGVDD19 pKa = 3.52 AVADD23 pKa = 3.67 GLAQVRR29 pKa = 11.84 VGDD32 pKa = 3.7 TAGYY36 pKa = 9.8 LPAGMVGSGSASVVMGACDD55 pKa = 3.67 TIDD58 pKa = 3.58 GLVEE62 pKa = 4.13 GVVEE66 pKa = 4.19 ALRR69 pKa = 11.84 DD70 pKa = 3.53 YY71 pKa = 11.01 SSNVGEE77 pKa = 4.62 TIAQFVATEE86 pKa = 4.11 DD87 pKa = 3.73 ANTVAFQNIANSSGVNN103 pKa = 3.29
Molecular weight: 10.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.694
IPC_protein 3.63
Toseland 3.427
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.35
Solomon 3.605
Lehninger 3.567
Nozaki 3.77
DTASelect 3.973
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.757
Patrickios 1.85
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A1D8B355|A0A1D8B355_9ACTO Uncharacterized protein OS=Pauljensenia hongkongensis OX=178339 GN=BH719_06740 PE=4 SV=1
MM1 pKa = 5.72 TTKK4 pKa = 9.84 RR5 pKa = 11.84 TFQPNNRR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 SKK16 pKa = 8.58 THH18 pKa = 5.46 GFRR21 pKa = 11.84 LRR23 pKa = 11.84 MSTRR27 pKa = 11.84 AGRR30 pKa = 11.84 AILANRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.83 GRR41 pKa = 11.84 AKK43 pKa = 10.68 LSAA46 pKa = 3.92
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1597
0
1597
546396
37
2402
342.1
36.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.424 ± 0.081
0.814 ± 0.018
6.059 ± 0.053
5.517 ± 0.059
2.942 ± 0.031
9.59 ± 0.062
1.906 ± 0.024
4.122 ± 0.043
2.262 ± 0.04
9.24 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.175 ± 0.027
2.078 ± 0.035
5.56 ± 0.051
2.957 ± 0.04
7.242 ± 0.067
6.079 ± 0.048
5.63 ± 0.046
8.654 ± 0.059
1.573 ± 0.027
2.178 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here