Amino acid dipepetide frequency for Staphylococcus phage vB_SauS_fPfSau02

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.234AlaAla: 2.234 ± 0.881
0.36AlaCys: 0.36 ± 0.185
2.379AlaAsp: 2.379 ± 0.486
4.253AlaGlu: 4.253 ± 0.487
1.369AlaPhe: 1.369 ± 0.278
3.243AlaGly: 3.243 ± 0.788
0.865AlaHis: 0.865 ± 0.314
4.325AlaIle: 4.325 ± 0.62
6.343AlaLys: 6.343 ± 1.234
4.685AlaLeu: 4.685 ± 0.85
1.369AlaMet: 1.369 ± 0.359
5.045AlaAsn: 5.045 ± 0.835
1.369AlaPro: 1.369 ± 0.323
1.658AlaGln: 1.658 ± 0.346
2.162AlaArg: 2.162 ± 0.366
4.613AlaSer: 4.613 ± 0.832
3.604AlaThr: 3.604 ± 0.442
2.883AlaVal: 2.883 ± 0.52
0.793AlaTrp: 0.793 ± 0.38
2.018AlaTyr: 2.018 ± 0.404
0.0AlaXaa: 0.0 ± 0.0
Cys
0.216CysAla: 0.216 ± 0.127
0.0CysCys: 0.0 ± 0.0
0.216CysAsp: 0.216 ± 0.127
0.432CysGlu: 0.432 ± 0.194
0.288CysPhe: 0.288 ± 0.142
0.144CysGly: 0.144 ± 0.112
0.144CysHis: 0.144 ± 0.102
0.288CysIle: 0.288 ± 0.137
0.577CysLys: 0.577 ± 0.236
0.36CysLeu: 0.36 ± 0.169
0.288CysMet: 0.288 ± 0.204
0.288CysAsn: 0.288 ± 0.136
0.0CysPro: 0.0 ± 0.0
0.144CysGln: 0.144 ± 0.097
0.288CysArg: 0.288 ± 0.175
0.072CysSer: 0.072 ± 0.08
0.216CysThr: 0.216 ± 0.141
0.216CysVal: 0.216 ± 0.129
0.0CysTrp: 0.0 ± 0.0
0.216CysTyr: 0.216 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
3.027AspAla: 3.027 ± 0.53
0.288AspCys: 0.288 ± 0.167
4.18AspAsp: 4.18 ± 0.735
4.613AspGlu: 4.613 ± 0.697
2.667AspPhe: 2.667 ± 0.456
4.036AspGly: 4.036 ± 0.662
0.505AspHis: 0.505 ± 0.146
5.262AspIle: 5.262 ± 0.678
6.847AspLys: 6.847 ± 0.843
5.838AspLeu: 5.838 ± 0.643
2.018AspMet: 2.018 ± 0.331
3.676AspAsn: 3.676 ± 0.485
0.793AspPro: 0.793 ± 0.246
1.586AspGln: 1.586 ± 0.29
2.523AspArg: 2.523 ± 0.396
3.532AspSer: 3.532 ± 0.496
3.316AspThr: 3.316 ± 0.483
4.253AspVal: 4.253 ± 0.533
0.937AspTrp: 0.937 ± 0.247
3.243AspTyr: 3.243 ± 0.624
0.0AspXaa: 0.0 ± 0.0
Glu
4.18GluAla: 4.18 ± 0.465
0.649GluCys: 0.649 ± 0.228
4.397GluAsp: 4.397 ± 0.636
6.415GluGlu: 6.415 ± 1.146
3.46GluPhe: 3.46 ± 0.644
3.099GluGly: 3.099 ± 0.4
1.081GluHis: 1.081 ± 0.326
5.838GluIle: 5.838 ± 0.957
8.866GluLys: 8.866 ± 0.866
7.208GluLeu: 7.208 ± 0.827
2.451GluMet: 2.451 ± 0.456
5.262GluAsn: 5.262 ± 0.769
1.369GluPro: 1.369 ± 0.307
4.036GluGln: 4.036 ± 0.722
2.955GluArg: 2.955 ± 0.613
3.892GluSer: 3.892 ± 0.595
4.469GluThr: 4.469 ± 0.675
3.892GluVal: 3.892 ± 0.48
1.081GluTrp: 1.081 ± 0.195
2.523GluTyr: 2.523 ± 0.523
0.0GluXaa: 0.0 ± 0.0
Phe
1.73PheAla: 1.73 ± 0.336
0.216PheCys: 0.216 ± 0.126
3.388PheAsp: 3.388 ± 0.52
3.099PheGlu: 3.099 ± 0.459
1.081PhePhe: 1.081 ± 0.303
3.316PheGly: 3.316 ± 0.591
0.649PheHis: 0.649 ± 0.194
2.955PheIle: 2.955 ± 0.646
4.541PheLys: 4.541 ± 0.678
2.162PheLeu: 2.162 ± 0.306
1.081PheMet: 1.081 ± 0.236
3.243PheAsn: 3.243 ± 0.488
0.721PhePro: 0.721 ± 0.284
0.937PheGln: 0.937 ± 0.234
1.009PheArg: 1.009 ± 0.254
1.73PheSer: 1.73 ± 0.277
1.73PheThr: 1.73 ± 0.373
2.234PheVal: 2.234 ± 0.437
0.216PheTrp: 0.216 ± 0.135
1.586PheTyr: 1.586 ± 0.387
0.0PheXaa: 0.0 ± 0.0
Gly
3.82GlyAla: 3.82 ± 1.15
0.288GlyCys: 0.288 ± 0.141
3.748GlyAsp: 3.748 ± 0.614
3.676GlyGlu: 3.676 ± 0.434
2.306GlyPhe: 2.306 ± 0.384
4.613GlyGly: 4.613 ± 1.372
1.442GlyHis: 1.442 ± 0.418
3.604GlyIle: 3.604 ± 0.568
5.766GlyLys: 5.766 ± 0.613
5.19GlyLeu: 5.19 ± 0.937
1.586GlyMet: 1.586 ± 0.469
2.883GlyAsn: 2.883 ± 0.659
0.793GlyPro: 0.793 ± 0.243
1.874GlyGln: 1.874 ± 0.429
1.802GlyArg: 1.802 ± 0.494
3.892GlySer: 3.892 ± 0.612
4.18GlyThr: 4.18 ± 0.514
4.397GlyVal: 4.397 ± 0.758
1.009GlyTrp: 1.009 ± 0.332
2.739GlyTyr: 2.739 ± 0.502
0.0GlyXaa: 0.0 ± 0.0
His
1.009HisAla: 1.009 ± 0.279
0.0HisCys: 0.0 ± 0.0
0.577HisAsp: 0.577 ± 0.206
1.081HisGlu: 1.081 ± 0.261
0.793HisPhe: 0.793 ± 0.24
1.009HisGly: 1.009 ± 0.275
0.36HisHis: 0.36 ± 0.196
1.225HisIle: 1.225 ± 0.424
1.081HisLys: 1.081 ± 0.253
1.442HisLeu: 1.442 ± 0.341
0.288HisMet: 0.288 ± 0.13
0.937HisAsn: 0.937 ± 0.224
0.649HisPro: 0.649 ± 0.183
0.793HisGln: 0.793 ± 0.2
0.793HisArg: 0.793 ± 0.225
0.865HisSer: 0.865 ± 0.195
1.225HisThr: 1.225 ± 0.301
0.793HisVal: 0.793 ± 0.293
0.288HisTrp: 0.288 ± 0.168
1.153HisTyr: 1.153 ± 0.283
0.0HisXaa: 0.0 ± 0.0
Ile
3.82IleAla: 3.82 ± 0.467
0.36IleCys: 0.36 ± 0.185
5.406IleAsp: 5.406 ± 0.798
6.271IleGlu: 6.271 ± 0.795
2.162IlePhe: 2.162 ± 0.498
3.388IleGly: 3.388 ± 0.607
1.586IleHis: 1.586 ± 0.355
4.18IleIle: 4.18 ± 0.67
7.784IleLys: 7.784 ± 0.818
4.325IleLeu: 4.325 ± 0.623
2.595IleMet: 2.595 ± 0.57
5.766IleAsn: 5.766 ± 0.706
1.946IlePro: 1.946 ± 0.266
2.162IleGln: 2.162 ± 0.399
3.099IleArg: 3.099 ± 0.445
4.901IleSer: 4.901 ± 0.624
4.397IleThr: 4.397 ± 0.571
4.469IleVal: 4.469 ± 0.851
0.36IleTrp: 0.36 ± 0.19
2.595IleTyr: 2.595 ± 0.511
0.0IleXaa: 0.0 ± 0.0
Lys
7.136LysAla: 7.136 ± 1.259
0.144LysCys: 0.144 ± 0.101
5.622LysAsp: 5.622 ± 0.619
9.658LysGlu: 9.658 ± 1.044
2.955LysPhe: 2.955 ± 0.391
5.117LysGly: 5.117 ± 0.996
1.802LysHis: 1.802 ± 0.346
6.919LysIle: 6.919 ± 0.872
8.433LysLys: 8.433 ± 1.06
8.938LysLeu: 8.938 ± 0.793
3.027LysMet: 3.027 ± 0.492
6.487LysAsn: 6.487 ± 0.724
2.306LysPro: 2.306 ± 0.398
5.478LysGln: 5.478 ± 0.55
3.676LysArg: 3.676 ± 0.562
5.91LysSer: 5.91 ± 1.312
5.694LysThr: 5.694 ± 0.617
5.55LysVal: 5.55 ± 0.642
1.874LysTrp: 1.874 ± 0.452
4.973LysTyr: 4.973 ± 0.781
0.0LysXaa: 0.0 ± 0.0
Leu
4.253LeuAla: 4.253 ± 0.852
0.288LeuCys: 0.288 ± 0.154
5.262LeuAsp: 5.262 ± 0.89
6.919LeuGlu: 6.919 ± 0.862
2.955LeuPhe: 2.955 ± 0.387
4.613LeuGly: 4.613 ± 1.094
1.153LeuHis: 1.153 ± 0.374
5.334LeuIle: 5.334 ± 0.571
8.866LeuLys: 8.866 ± 1.363
6.703LeuLeu: 6.703 ± 0.966
1.514LeuMet: 1.514 ± 0.234
5.55LeuAsn: 5.55 ± 0.555
2.955LeuPro: 2.955 ± 0.595
3.46LeuGln: 3.46 ± 0.661
3.027LeuArg: 3.027 ± 0.436
5.262LeuSer: 5.262 ± 0.643
5.478LeuThr: 5.478 ± 0.684
3.676LeuVal: 3.676 ± 0.403
0.505LeuTrp: 0.505 ± 0.229
3.46LeuTyr: 3.46 ± 0.783
0.0LeuXaa: 0.0 ± 0.0
Met
1.225MetAla: 1.225 ± 0.261
0.216MetCys: 0.216 ± 0.13
1.514MetAsp: 1.514 ± 0.394
1.369MetGlu: 1.369 ± 0.288
0.865MetPhe: 0.865 ± 0.27
1.297MetGly: 1.297 ± 0.586
0.36MetHis: 0.36 ± 0.171
1.225MetIle: 1.225 ± 0.274
2.955MetLys: 2.955 ± 0.511
2.451MetLeu: 2.451 ± 0.517
0.288MetMet: 0.288 ± 0.12
2.09MetAsn: 2.09 ± 0.469
0.865MetPro: 0.865 ± 0.218
1.946MetGln: 1.946 ± 0.452
1.081MetArg: 1.081 ± 0.229
2.234MetSer: 2.234 ± 0.445
2.306MetThr: 2.306 ± 0.363
1.153MetVal: 1.153 ± 0.247
0.432MetTrp: 0.432 ± 0.167
0.865MetTyr: 0.865 ± 0.239
0.0MetXaa: 0.0 ± 0.0
Asn
3.604AsnAla: 3.604 ± 0.568
0.144AsnCys: 0.144 ± 0.139
4.325AsnAsp: 4.325 ± 0.48
5.406AsnGlu: 5.406 ± 0.767
1.297AsnPhe: 1.297 ± 0.262
5.694AsnGly: 5.694 ± 0.619
1.009AsnHis: 1.009 ± 0.326
4.036AsnIle: 4.036 ± 0.535
7.784AsnLys: 7.784 ± 0.792
4.973AsnLeu: 4.973 ± 0.501
1.297AsnMet: 1.297 ± 0.275
4.685AsnAsn: 4.685 ± 0.634
2.306AsnPro: 2.306 ± 0.359
2.523AsnGln: 2.523 ± 0.476
2.955AsnArg: 2.955 ± 0.461
3.964AsnSer: 3.964 ± 0.669
4.036AsnThr: 4.036 ± 0.519
3.243AsnVal: 3.243 ± 0.617
1.369AsnTrp: 1.369 ± 0.367
2.667AsnTyr: 2.667 ± 0.511
0.0AsnXaa: 0.0 ± 0.0
Pro
0.865ProAla: 0.865 ± 0.237
0.144ProCys: 0.144 ± 0.1
1.369ProAsp: 1.369 ± 0.305
1.802ProGlu: 1.802 ± 0.366
1.369ProPhe: 1.369 ± 0.442
1.442ProGly: 1.442 ± 0.332
0.216ProHis: 0.216 ± 0.127
1.802ProIle: 1.802 ± 0.368
2.451ProLys: 2.451 ± 0.55
2.018ProLeu: 2.018 ± 0.464
0.937ProMet: 0.937 ± 0.256
1.946ProAsn: 1.946 ± 0.43
0.505ProPro: 0.505 ± 0.205
1.153ProGln: 1.153 ± 0.226
1.009ProArg: 1.009 ± 0.247
2.234ProSer: 2.234 ± 0.385
1.802ProThr: 1.802 ± 0.352
1.153ProVal: 1.153 ± 0.3
0.216ProTrp: 0.216 ± 0.11
1.297ProTyr: 1.297 ± 0.316
0.0ProXaa: 0.0 ± 0.0
Gln
2.667GlnAla: 2.667 ± 0.335
0.216GlnCys: 0.216 ± 0.142
2.451GlnAsp: 2.451 ± 0.467
3.099GlnGlu: 3.099 ± 0.462
1.874GlnPhe: 1.874 ± 0.319
1.946GlnGly: 1.946 ± 0.376
0.649GlnHis: 0.649 ± 0.199
3.316GlnIle: 3.316 ± 0.47
3.46GlnLys: 3.46 ± 0.414
4.108GlnLeu: 4.108 ± 0.54
0.865GlnMet: 0.865 ± 0.245
2.162GlnAsn: 2.162 ± 0.438
1.297GlnPro: 1.297 ± 0.289
2.09GlnGln: 2.09 ± 0.688
2.306GlnArg: 2.306 ± 0.364
2.523GlnSer: 2.523 ± 0.374
1.369GlnThr: 1.369 ± 0.435
2.595GlnVal: 2.595 ± 0.443
0.216GlnTrp: 0.216 ± 0.107
1.946GlnTyr: 1.946 ± 0.421
0.0GlnXaa: 0.0 ± 0.0
Arg
2.379ArgAla: 2.379 ± 0.586
0.072ArgCys: 0.072 ± 0.071
3.316ArgAsp: 3.316 ± 0.438
2.595ArgGlu: 2.595 ± 0.497
1.946ArgPhe: 1.946 ± 0.341
2.162ArgGly: 2.162 ± 0.287
1.009ArgHis: 1.009 ± 0.265
3.676ArgIle: 3.676 ± 0.555
3.964ArgLys: 3.964 ± 0.438
2.883ArgLeu: 2.883 ± 0.485
1.081ArgMet: 1.081 ± 0.256
2.523ArgAsn: 2.523 ± 0.438
0.865ArgPro: 0.865 ± 0.273
1.658ArgGln: 1.658 ± 0.306
1.442ArgArg: 1.442 ± 0.396
1.442ArgSer: 1.442 ± 0.326
2.018ArgThr: 2.018 ± 0.466
1.658ArgVal: 1.658 ± 0.357
0.144ArgTrp: 0.144 ± 0.102
2.523ArgTyr: 2.523 ± 0.479
0.0ArgXaa: 0.0 ± 0.0
Ser
3.748SerAla: 3.748 ± 0.815
0.216SerCys: 0.216 ± 0.133
4.541SerAsp: 4.541 ± 0.597
4.036SerGlu: 4.036 ± 0.595
2.523SerPhe: 2.523 ± 0.475
4.036SerGly: 4.036 ± 1.076
0.577SerHis: 0.577 ± 0.212
3.532SerIle: 3.532 ± 0.517
6.054SerLys: 6.054 ± 1.163
4.541SerLeu: 4.541 ± 0.517
2.018SerMet: 2.018 ± 0.377
4.829SerAsn: 4.829 ± 0.623
1.73SerPro: 1.73 ± 0.418
2.811SerGln: 2.811 ± 0.369
2.523SerArg: 2.523 ± 0.432
3.82SerSer: 3.82 ± 0.784
2.811SerThr: 2.811 ± 0.438
3.748SerVal: 3.748 ± 0.567
0.649SerTrp: 0.649 ± 0.214
2.09SerTyr: 2.09 ± 0.373
0.0SerXaa: 0.0 ± 0.0
Thr
3.316ThrAla: 3.316 ± 0.632
0.288ThrCys: 0.288 ± 0.151
3.46ThrAsp: 3.46 ± 0.581
4.036ThrGlu: 4.036 ± 0.562
2.739ThrPhe: 2.739 ± 0.404
4.613ThrGly: 4.613 ± 0.676
1.225ThrHis: 1.225 ± 0.297
5.117ThrIle: 5.117 ± 0.726
5.694ThrLys: 5.694 ± 0.832
4.036ThrLeu: 4.036 ± 0.6
0.865ThrMet: 0.865 ± 0.274
3.82ThrAsn: 3.82 ± 0.654
2.595ThrPro: 2.595 ± 0.342
2.018ThrGln: 2.018 ± 0.312
2.018ThrArg: 2.018 ± 0.339
2.523ThrSer: 2.523 ± 0.47
2.883ThrThr: 2.883 ± 0.49
4.108ThrVal: 4.108 ± 0.471
0.649ThrTrp: 0.649 ± 0.259
2.162ThrTyr: 2.162 ± 0.448
0.0ThrXaa: 0.0 ± 0.0
Val
3.82ValAla: 3.82 ± 0.443
0.216ValCys: 0.216 ± 0.124
4.036ValAsp: 4.036 ± 0.513
5.19ValGlu: 5.19 ± 0.682
2.234ValPhe: 2.234 ± 0.472
3.171ValGly: 3.171 ± 0.578
0.793ValHis: 0.793 ± 0.196
4.757ValIle: 4.757 ± 0.63
5.478ValLys: 5.478 ± 0.631
4.613ValLeu: 4.613 ± 0.49
1.369ValMet: 1.369 ± 0.282
3.027ValAsn: 3.027 ± 0.552
1.297ValPro: 1.297 ± 0.345
2.018ValGln: 2.018 ± 0.381
1.874ValArg: 1.874 ± 0.478
3.604ValSer: 3.604 ± 0.528
3.388ValThr: 3.388 ± 0.678
3.388ValVal: 3.388 ± 0.652
0.505ValTrp: 0.505 ± 0.209
2.523ValTyr: 2.523 ± 0.541
0.0ValXaa: 0.0 ± 0.0
Trp
0.505TrpAla: 0.505 ± 0.171
0.0TrpCys: 0.0 ± 0.0
0.288TrpAsp: 0.288 ± 0.22
0.937TrpGlu: 0.937 ± 0.285
1.153TrpPhe: 1.153 ± 0.383
0.288TrpGly: 0.288 ± 0.15
0.144TrpHis: 0.144 ± 0.115
1.297TrpIle: 1.297 ± 0.332
0.865TrpLys: 0.865 ± 0.244
0.937TrpLeu: 0.937 ± 0.298
0.505TrpMet: 0.505 ± 0.228
0.865TrpAsn: 0.865 ± 0.259
0.072TrpPro: 0.072 ± 0.071
0.432TrpGln: 0.432 ± 0.172
0.432TrpArg: 0.432 ± 0.187
0.937TrpSer: 0.937 ± 0.382
0.505TrpThr: 0.505 ± 0.182
0.865TrpVal: 0.865 ± 0.28
0.144TrpTrp: 0.144 ± 0.138
0.793TrpTyr: 0.793 ± 0.254
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.018TyrAla: 2.018 ± 0.313
0.288TyrCys: 0.288 ± 0.153
2.739TyrAsp: 2.739 ± 0.725
2.234TyrGlu: 2.234 ± 0.476
1.586TyrPhe: 1.586 ± 0.502
2.379TyrGly: 2.379 ± 0.488
0.793TyrHis: 0.793 ± 0.26
2.883TyrIle: 2.883 ± 0.556
3.892TyrLys: 3.892 ± 0.559
3.82TyrLeu: 3.82 ± 0.609
1.225TyrMet: 1.225 ± 0.248
2.234TyrAsn: 2.234 ± 0.461
1.297TyrPro: 1.297 ± 0.313
2.379TyrGln: 2.379 ± 0.372
2.379TyrArg: 2.379 ± 0.526
2.883TyrSer: 2.883 ± 0.433
2.739TyrThr: 2.739 ± 0.532
3.027TyrVal: 3.027 ± 0.472
0.577TyrTrp: 0.577 ± 0.172
1.73TyrTyr: 1.73 ± 0.472
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (13875 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski