Streptococcus phage Javan509
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AK41|A0A4D6AK41_9CAUD Uncharacterized protein OS=Streptococcus phage Javan509 OX=2548222 GN=Javan509_0042 PE=4 SV=1
MM1 pKa = 7.89 IEE3 pKa = 4.22 LTQKK7 pKa = 9.53 QADD10 pKa = 3.73 YY11 pKa = 11.45 VEE13 pKa = 5.18 FVRR16 pKa = 11.84 KK17 pKa = 8.37 TIVDD21 pKa = 3.65 PDD23 pKa = 3.48 MMEE26 pKa = 4.18 FAVNTQLISYY36 pKa = 8.87 ALLVGYY42 pKa = 9.09 CIVDD46 pKa = 3.6 EE47 pKa = 4.67 EE48 pKa = 4.57 VAKK51 pKa = 10.96
Molecular weight: 5.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.21
IPC2_protein 4.279
IPC_protein 4.075
Toseland 3.91
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.961
Rodwell 3.923
Grimsley 3.834
Solomon 4.024
Lehninger 3.986
Nozaki 4.177
DTASelect 4.317
Thurlkill 3.961
EMBOSS 3.973
Sillero 4.19
Patrickios 3.249
IPC_peptide 4.024
IPC2_peptide 4.177
IPC2.peptide.svr19 4.1
Protein with the highest isoelectric point:
>tr|A0A4D6AIM3|A0A4D6AIM3_9CAUD Hyaluronidase OS=Streptococcus phage Javan509 OX=2548222 GN=Javan509_0007 PE=4 SV=1
MM1 pKa = 7.4 NKK3 pKa = 10.29 LEE5 pKa = 4.69 LFLLATTVILAVIARR20 pKa = 11.84 VQHH23 pKa = 5.44 EE24 pKa = 4.77 VIKK27 pKa = 10.66 KK28 pKa = 9.96 HH29 pKa = 5.97 NSPEE33 pKa = 3.91 NKK35 pKa = 9.18 RR36 pKa = 11.84 KK37 pKa = 9.67 IFRR40 pKa = 11.84 EE41 pKa = 4.02 VALKK45 pKa = 10.61 NSKK48 pKa = 9.36 GWSKK52 pKa = 10.54 KK53 pKa = 7.07 RR54 pKa = 11.84 ARR56 pKa = 11.84 GGVVSS61 pKa = 4.31
Molecular weight: 6.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.231
IPC2_protein 9.911
IPC_protein 10.599
Toseland 11.374
ProMoST 11.286
Dawson 11.403
Bjellqvist 11.14
Wikipedia 11.652
Rodwell 11.754
Grimsley 11.418
Solomon 11.637
Lehninger 11.594
Nozaki 11.345
DTASelect 11.14
Thurlkill 11.345
EMBOSS 11.798
Sillero 11.345
Patrickios 11.506
IPC_peptide 11.652
IPC2_peptide 9.97
IPC2.peptide.svr19 8.849
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
10311
50
787
202.2
22.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.439 ± 0.534
0.64 ± 0.111
6.605 ± 0.358
7.08 ± 0.549
3.967 ± 0.192
6.605 ± 0.462
1.309 ± 0.143
7.196 ± 0.202
9.398 ± 0.458
8.098 ± 0.322
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.357 ± 0.155
5.295 ± 0.211
2.842 ± 0.205
3.802 ± 0.251
3.705 ± 0.312
6.517 ± 0.467
5.965 ± 0.192
6.255 ± 0.256
1.164 ± 0.126
3.763 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here