Mariniblastus fucicola
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5083 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B9P529|A0A5B9P529_9BACT Trigger factor OS=Mariniblastus fucicola OX=980251 GN=tig PE=3 SV=1
MM1 pKa = 7.26 NRR3 pKa = 11.84 RR4 pKa = 11.84 IKK6 pKa = 10.69 SKK8 pKa = 10.89 SRR10 pKa = 11.84 FQSYY14 pKa = 7.71 QQLEE18 pKa = 4.45 AKK20 pKa = 10.12 NLLAAIYY27 pKa = 8.94 HH28 pKa = 6.0 NPDD31 pKa = 2.87 SGILYY36 pKa = 9.97 IAGDD40 pKa = 3.34 SGANVGQVSVSGDD53 pKa = 3.34 NVVAQIDD60 pKa = 4.1 GQSYY64 pKa = 10.56 SGASDD69 pKa = 3.87 DD70 pKa = 3.63 VTDD73 pKa = 5.41 IIFIGYY79 pKa = 9.56 DD80 pKa = 3.39 GNDD83 pKa = 3.19 TFTNLSDD90 pKa = 5.01 LDD92 pKa = 3.75 SSMYY96 pKa = 10.72 GHH98 pKa = 7.91 LGNDD102 pKa = 3.43 TLTGGGGDD110 pKa = 3.95 DD111 pKa = 4.02 NLVGGAGNDD120 pKa = 3.94 TIQGLDD126 pKa = 3.31 GDD128 pKa = 4.41 DD129 pKa = 4.44 RR130 pKa = 11.84 IVGADD135 pKa = 3.59 GNDD138 pKa = 3.5 TLEE141 pKa = 4.81 GGDD144 pKa = 3.66 GEE146 pKa = 4.98 DD147 pKa = 4.95 RR148 pKa = 11.84 IFGTAGTNTIYY159 pKa = 10.91 GGAGDD164 pKa = 3.77 DD165 pKa = 3.97 TIYY168 pKa = 10.96 GSEE171 pKa = 4.51 DD172 pKa = 3.21 ADD174 pKa = 4.55 EE175 pKa = 5.1 IYY177 pKa = 11.28 GDD179 pKa = 3.66 AGADD183 pKa = 3.34 KK184 pKa = 10.33 IYY186 pKa = 11.1 ALGGDD191 pKa = 4.51 DD192 pKa = 4.66 ILYY195 pKa = 8.98 TGTGGVEE202 pKa = 3.7 GGSFDD207 pKa = 4.78 EE208 pKa = 5.41 GDD210 pKa = 3.26 LAMGHH215 pKa = 6.34 GGDD218 pKa = 4.12 DD219 pKa = 3.41 EE220 pKa = 6.02 FYY222 pKa = 11.1 GSTGLNIFYY231 pKa = 10.67 GGDD234 pKa = 3.42 GDD236 pKa = 5.55 DD237 pKa = 3.74 IMVGGSGEE245 pKa = 4.08 NRR247 pKa = 11.84 MHH249 pKa = 6.35 GQAGDD254 pKa = 3.62 DD255 pKa = 3.7 TLTGGEE261 pKa = 4.49 KK262 pKa = 10.17 GDD264 pKa = 3.91 YY265 pKa = 7.46 MTGADD270 pKa = 4.9 GDD272 pKa = 4.07 DD273 pKa = 3.74 TFDD276 pKa = 3.32 GRR278 pKa = 11.84 GGVDD282 pKa = 3.42 YY283 pKa = 11.28 YY284 pKa = 11.84 NVGDD288 pKa = 3.99 GSDD291 pKa = 3.73 TIVYY295 pKa = 8.42 SQSYY299 pKa = 9.08 VPTDD303 pKa = 3.05 VHH305 pKa = 5.17 VTNNGGTAEE314 pKa = 3.98 TRR316 pKa = 11.84 IQNEE320 pKa = 4.3 LVSNATWVQFADD332 pKa = 3.85 RR333 pKa = 11.84 TISADD338 pKa = 3.0 QAYY341 pKa = 10.06 YY342 pKa = 9.42 LTVDD346 pKa = 3.42 EE347 pKa = 5.31 TNFSSLNGYY356 pKa = 8.95 RR357 pKa = 11.84 VSSSVGFVSKK367 pKa = 10.65 PSDD370 pKa = 3.51 LADD373 pKa = 4.02 FAYY376 pKa = 10.45 NWSLQMASAGTLSHH390 pKa = 5.8 STEE393 pKa = 3.87 SDD395 pKa = 3.14 RR396 pKa = 11.84 ASLYY400 pKa = 10.77 VGDD403 pKa = 4.45 RR404 pKa = 11.84 SAVGEE409 pKa = 4.14 NVAMVSDD416 pKa = 4.55 TGQSDD421 pKa = 3.96 VQIANYY427 pKa = 9.47 FLSLWQSSSAHH438 pKa = 5.92 NSNLLNSNYY447 pKa = 9.78 QEE449 pKa = 3.96 VGIGIVKK456 pKa = 10.46 SGGAWWATQIFMGG469 pKa = 4.72
Molecular weight: 49.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.656
IPC_protein 3.719
Toseland 3.465
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.694
Rodwell 3.528
Grimsley 3.376
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.139
Thurlkill 3.528
EMBOSS 3.694
Sillero 3.834
Patrickios 0.731
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A5B9PK37|A0A5B9PK37_9BACT Anhydro-N-acetylmuramic acid kinase OS=Mariniblastus fucicola OX=980251 GN=MFFC18_27390 PE=4 SV=1
MM1 pKa = 7.32 NRR3 pKa = 11.84 TKK5 pKa = 10.53 FFSTIALLCCCFLASPAIAQDD26 pKa = 4.52 ISGQWSGGWHH36 pKa = 5.83 SGANNHH42 pKa = 6.78 RR43 pKa = 11.84 GNIKK47 pKa = 9.42 ATFCQIDD54 pKa = 4.0 SSTMQAKK61 pKa = 9.8 FRR63 pKa = 11.84 GTFAKK68 pKa = 10.24 VIPFRR73 pKa = 11.84 YY74 pKa = 5.16 TTRR77 pKa = 11.84 LNIVSQQPGLTVMAGSRR94 pKa = 11.84 RR95 pKa = 11.84 LPLGGEE101 pKa = 4.5 FKK103 pKa = 10.98 YY104 pKa = 10.46 QISMTDD110 pKa = 2.81 QCFNGTFSSRR120 pKa = 11.84 RR121 pKa = 11.84 NHH123 pKa = 5.97 GQFVMQRR130 pKa = 11.84 QQ131 pKa = 3.29
Molecular weight: 14.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.545
IPC_protein 10.116
Toseland 10.57
ProMoST 10.218
Dawson 10.672
Bjellqvist 10.379
Wikipedia 10.847
Rodwell 10.935
Grimsley 10.716
Solomon 10.774
Lehninger 10.745
Nozaki 10.628
DTASelect 10.35
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.613
Patrickios 10.716
IPC_peptide 10.774
IPC2_peptide 9.765
IPC2.peptide.svr19 8.463
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5083
0
5083
1929523
29
7782
379.6
41.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.864 ± 0.033
1.122 ± 0.018
6.426 ± 0.061
6.556 ± 0.035
4.262 ± 0.023
7.484 ± 0.053
1.963 ± 0.02
5.545 ± 0.025
4.577 ± 0.056
9.189 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.361 ± 0.022
3.847 ± 0.027
4.527 ± 0.025
3.913 ± 0.03
5.785 ± 0.053
7.128 ± 0.034
5.494 ± 0.063
7.2 ± 0.034
1.406 ± 0.017
2.354 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here