Microbacterium phage Smarties

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Metamorphoovirus; unclassified Metamorphoovirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VIB6|A0A649VIB6_9CAUD Uncharacterized protein OS=Microbacterium phage Smarties OX=2656553 GN=83 PE=4 SV=1
MM1 pKa = 7.93IMALHH6 pKa = 7.23DD7 pKa = 4.42SPVSQSDD14 pKa = 3.31VDD16 pKa = 4.84DD17 pKa = 5.09VINDD21 pKa = 3.2AHH23 pKa = 5.63EE24 pKa = 4.42QIDD27 pKa = 3.83NGGSRR32 pKa = 11.84WPGMSYY38 pKa = 10.45EE39 pKa = 4.1QGLVAGLQWVTGEE52 pKa = 4.45TDD54 pKa = 3.13DD55 pKa = 5.61HH56 pKa = 6.72PYY58 pKa = 10.82EE59 pKa = 5.96DD60 pKa = 4.09EE61 pKa = 4.08

Molecular weight: 6.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VGD7|A0A649VGD7_9CAUD Head-to-tail adaptor OS=Microbacterium phage Smarties OX=2656553 GN=39 PE=4 SV=1
MM1 pKa = 7.34SARR4 pKa = 11.84VTTVIRR10 pKa = 11.84CNRR13 pKa = 11.84IGCTARR19 pKa = 11.84LEE21 pKa = 4.61HH22 pKa = 6.92PTTPPAARR30 pKa = 11.84AEE32 pKa = 4.16AKK34 pKa = 10.35RR35 pKa = 11.84LGWKK39 pKa = 9.92VDD41 pKa = 3.35VASWNRR47 pKa = 11.84RR48 pKa = 11.84DD49 pKa = 3.8RR50 pKa = 11.84EE51 pKa = 4.36DD52 pKa = 3.23YY53 pKa = 10.92CPEE56 pKa = 3.59HH57 pKa = 6.97RR58 pKa = 11.84RR59 pKa = 3.59

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

17313

37

956

173.1

18.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.904 ± 0.491

0.97 ± 0.135

6.562 ± 0.394

6.486 ± 0.386

2.426 ± 0.134

8.768 ± 0.351

1.952 ± 0.174

4.482 ± 0.256

2.42 ± 0.21

7.601 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.137

2.68 ± 0.204

6.29 ± 0.276

3.333 ± 0.16

7.035 ± 0.41

5.66 ± 0.273

7.035 ± 0.291

7.382 ± 0.203

2.074 ± 0.159

2.432 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski