Chitinophaga pinensis (strain ATCC 43595 / DSM 2588 / LMG 13176 / NBRC 15968 / NCIMB 11800 / UQM 2034)
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7179 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7PSK9|C7PSK9_CHIPD Lipid-A-disaccharide synthase OS=Chitinophaga pinensis (strain ATCC 43595 / DSM 2588 / LMG 13176 / NBRC 15968 / NCIMB 11800 / UQM 2034) OX=485918 GN=Cpin_7057 PE=4 SV=1
MM1 pKa = 7.69 EE2 pKa = 5.08 EE3 pKa = 4.33 FYY5 pKa = 11.22 DD6 pKa = 4.49 AASEE10 pKa = 4.38 KK11 pKa = 9.76 YY12 pKa = 9.79 KK13 pKa = 10.75 KK14 pKa = 10.57 YY15 pKa = 10.61 LLQVVYY21 pKa = 10.84 NDD23 pKa = 3.13 EE24 pKa = 4.05 TYY26 pKa = 9.25 YY27 pKa = 10.36 TVSGADD33 pKa = 3.34 LSDD36 pKa = 3.96 NEE38 pKa = 4.28 ATRR41 pKa = 11.84 LLTDD45 pKa = 3.94 ADD47 pKa = 4.21 GKK49 pKa = 10.17 ICLYY53 pKa = 11.13 ADD55 pKa = 4.13 LPSLRR60 pKa = 11.84 KK61 pKa = 10.02 GIEE64 pKa = 3.86 AGVVTFDD71 pKa = 4.71 TPNLQAWGKK80 pKa = 10.11 DD81 pKa = 3.03 INEE84 pKa = 4.09 TDD86 pKa = 3.22 TAYY89 pKa = 9.91 TGVDD93 pKa = 3.99 FFSLKK98 pKa = 10.28 SEE100 pKa = 4.14 HH101 pKa = 7.03 LEE103 pKa = 4.45 ADD105 pKa = 4.32 DD106 pKa = 6.32 DD107 pKa = 4.37 PLLYY111 pKa = 10.31 EE112 pKa = 4.46 IYY114 pKa = 10.45 GALGVVRR121 pKa = 11.84 DD122 pKa = 3.84 YY123 pKa = 11.89 AEE125 pKa = 3.95 QEE127 pKa = 4.08 NATEE131 pKa = 5.0 LLTLLDD137 pKa = 3.88 SPIVNEE143 pKa = 3.89 YY144 pKa = 10.26 MEE146 pKa = 4.3 ICADD150 pKa = 4.05 LFLWSSDD157 pKa = 2.78 TDD159 pKa = 3.73 SFRR162 pKa = 11.84 EE163 pKa = 4.02 DD164 pKa = 3.01 FDD166 pKa = 5.35 FNAFVPVLGQIYY178 pKa = 8.4 TLLEE182 pKa = 3.66 PRR184 pKa = 11.84 LRR186 pKa = 11.84 VVV188 pKa = 3.33
Molecular weight: 21.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.643
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.554
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.77
Sillero 3.973
Patrickios 1.163
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|C7PEB3|C7PEB3_CHIPD Uncharacterized protein OS=Chitinophaga pinensis (strain ATCC 43595 / DSM 2588 / LMG 13176 / NBRC 15968 / NCIMB 11800 / UQM 2034) OX=485918 GN=Cpin_5904 PE=4 SV=1
MM1 pKa = 7.04 SRR3 pKa = 11.84 EE4 pKa = 3.96 YY5 pKa = 10.17 FKK7 pKa = 11.21 SIIRR11 pKa = 11.84 TLRR14 pKa = 11.84 VSFQVLQNNDD24 pKa = 3.75 PLRR27 pKa = 11.84 LAGATAFFASFALPPILLILLQVFGLLFDD56 pKa = 3.8 RR57 pKa = 11.84 RR58 pKa = 11.84 SIGRR62 pKa = 11.84 QLIGRR67 pKa = 11.84 LGEE70 pKa = 4.2 LVGKK74 pKa = 10.29 DD75 pKa = 2.87 SAQQVVITLRR85 pKa = 11.84 GFRR88 pKa = 11.84 GLAQTWPIAIILFVFLLFVATTLLKK113 pKa = 10.65 VIRR116 pKa = 11.84 SSLNQVWMIRR126 pKa = 11.84 IAVRR130 pKa = 11.84 EE131 pKa = 4.11 SFLDD135 pKa = 3.48 SLLGRR140 pKa = 11.84 LRR142 pKa = 11.84 SLIVIGAAGLLFLASVLGEE161 pKa = 4.13 SAHH164 pKa = 6.74 AFLGNYY170 pKa = 8.93 VNEE173 pKa = 4.79 LFPSAAFIFSGVFTQLVSLLIVTCWFAVLFRR204 pKa = 11.84 YY205 pKa = 10.09 LPDD208 pKa = 4.5 ARR210 pKa = 11.84 PTWKK214 pKa = 10.44 VAFSGALLTGVLFSIGKK231 pKa = 9.16 LVLRR235 pKa = 11.84 RR236 pKa = 11.84 LLYY239 pKa = 10.55 GGNIGILYY247 pKa = 8.77 GASASAVLLMLFIFYY262 pKa = 10.51 SAIIFYY268 pKa = 10.71 YY269 pKa = 9.89 GAAFTKK275 pKa = 9.16 VWSEE279 pKa = 4.03 FKK281 pKa = 10.35 HH282 pKa = 7.39 RR283 pKa = 11.84 PMQPLRR289 pKa = 11.84 HH290 pKa = 4.99 ATFYY294 pKa = 11.14 EE295 pKa = 4.13 LADD298 pKa = 3.51 VEE300 pKa = 4.43 ARR302 pKa = 3.61
Molecular weight: 33.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.897
IPC_protein 10.804
Toseland 10.584
ProMoST 10.584
Dawson 10.745
Bjellqvist 10.526
Wikipedia 11.008
Rodwell 10.847
Grimsley 10.833
Solomon 10.862
Lehninger 10.818
Nozaki 10.57
DTASelect 10.526
Thurlkill 10.628
EMBOSS 11.008
Sillero 10.672
Patrickios 10.496
IPC_peptide 10.862
IPC2_peptide 9.56
IPC2.peptide.svr19 8.413
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7179
0
7179
2679255
30
9175
373.2
41.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.109 ± 0.036
0.837 ± 0.012
5.364 ± 0.023
5.477 ± 0.036
4.524 ± 0.025
6.966 ± 0.03
2.039 ± 0.019
6.611 ± 0.026
5.951 ± 0.037
9.518 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.372 ± 0.018
5.113 ± 0.041
4.121 ± 0.018
4.091 ± 0.021
4.475 ± 0.025
6.201 ± 0.028
6.188 ± 0.059
6.533 ± 0.032
1.282 ± 0.013
4.228 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here