STL polyomavirus
Average proteome isoelectric point is 5.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L7RFL1|L7RFL1_9POLY Small T antigen OS=STL polyomavirus OX=1277649 PE=4 SV=1
MM1 pKa = 7.57 GLLISVFLDD10 pKa = 3.43 LLDD13 pKa = 4.26 LVAITSFDD21 pKa = 3.25 VEE23 pKa = 4.88 AIVSGDD29 pKa = 3.05 AAAIVASQIAAQTVLEE45 pKa = 4.31 SAEE48 pKa = 4.05 LTEE51 pKa = 4.29 VLSSFGLTEE60 pKa = 3.98 ASYY63 pKa = 11.0 AALSAFPTAFQEE75 pKa = 4.63 AVDD78 pKa = 4.43 NVLLLQTLSGLSSYY92 pKa = 10.53 IAVGLSLQTYY102 pKa = 7.59 EE103 pKa = 5.21 IPDD106 pKa = 3.67 TNMALQIWTPDD117 pKa = 3.08 ILDD120 pKa = 4.47 EE121 pKa = 4.98 IIPGYY126 pKa = 10.33 RR127 pKa = 11.84 QFQYY131 pKa = 11.38 YY132 pKa = 10.8 LDD134 pKa = 4.04 VLSGWGQSLVRR145 pKa = 11.84 TVGRR149 pKa = 11.84 AFWEE153 pKa = 4.03 AVIDD157 pKa = 3.91 EE158 pKa = 4.65 TTSRR162 pKa = 11.84 LQRR165 pKa = 11.84 IGSSSLQAAEE175 pKa = 3.99 NAGLQTVEE183 pKa = 4.38 NVRR186 pKa = 11.84 QAIVTVVEE194 pKa = 3.87 NARR197 pKa = 11.84 WAIRR201 pKa = 11.84 TPVNIYY207 pKa = 10.83 GSLSDD212 pKa = 3.91 YY213 pKa = 10.83 YY214 pKa = 11.58 SRR216 pKa = 11.84 LPGLNPAQLRR226 pKa = 11.84 EE227 pKa = 4.3 LNSRR231 pKa = 11.84 LQSNAHH237 pKa = 6.44 NIQIDD242 pKa = 4.25 SYY244 pKa = 10.63 SAPDD248 pKa = 3.71 SLSGQYY254 pKa = 9.51 IEE256 pKa = 5.75 RR257 pKa = 11.84 PQSYY261 pKa = 9.79 SGINMRR267 pKa = 11.84 PVPDD271 pKa = 3.48 WLLPLILGILGDD283 pKa = 3.92 LSPFFEE289 pKa = 4.92 EE290 pKa = 3.88 IVEE293 pKa = 4.16 EE294 pKa = 4.33 VEE296 pKa = 3.97 NGSKK300 pKa = 10.54 KK301 pKa = 10.61 KK302 pKa = 10.2 II303 pKa = 3.59
Molecular weight: 33.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.245
IPC2_protein 4.215
IPC_protein 4.151
Toseland 3.973
ProMoST 4.266
Dawson 4.113
Bjellqvist 4.266
Wikipedia 3.986
Rodwell 3.986
Grimsley 3.884
Solomon 4.101
Lehninger 4.062
Nozaki 4.215
DTASelect 4.38
Thurlkill 3.999
EMBOSS 3.999
Sillero 4.266
Patrickios 3.414
IPC_peptide 4.113
IPC2_peptide 4.253
IPC2.peptide.svr19 4.187
Protein with the highest isoelectric point:
>tr|L7RFL1|L7RFL1_9POLY Small T antigen OS=STL polyomavirus OX=1277649 PE=4 SV=1
MM1 pKa = 7.64 DD2 pKa = 3.57 QALSRR7 pKa = 11.84 AEE9 pKa = 4.1 AKK11 pKa = 10.56 EE12 pKa = 3.68 LMGLLGLPEE21 pKa = 4.96 DD22 pKa = 3.61 SWGNVPLITYY32 pKa = 8.79 RR33 pKa = 11.84 FRR35 pKa = 11.84 QKK37 pKa = 11.04 SKK39 pKa = 10.12 IYY41 pKa = 10.69 HH42 pKa = 6.42 PDD44 pKa = 2.91 KK45 pKa = 11.13 GGNEE49 pKa = 3.92 EE50 pKa = 3.94 TMKK53 pKa = 10.87 RR54 pKa = 11.84 MTEE57 pKa = 4.27 LYY59 pKa = 10.96 SRR61 pKa = 11.84 MQNTLQNLRR70 pKa = 11.84 SSNEE74 pKa = 3.87 NEE76 pKa = 3.86 NMYY79 pKa = 10.56 PPVRR83 pKa = 11.84 MLLLTDD89 pKa = 4.09 TFTLGEE95 pKa = 3.98 LLGPQFEE102 pKa = 4.86 SKK104 pKa = 10.83 VIFIWPTCAKK114 pKa = 9.57 CRR116 pKa = 11.84 YY117 pKa = 8.27 RR118 pKa = 11.84 TFCKK122 pKa = 10.18 CVCCILKK129 pKa = 10.04 RR130 pKa = 11.84 QHH132 pKa = 7.26 DD133 pKa = 4.51 EE134 pKa = 3.73 IKK136 pKa = 10.44 KK137 pKa = 9.74 VRR139 pKa = 11.84 NKK141 pKa = 10.56 PCVTWGEE148 pKa = 4.84 CYY150 pKa = 10.73 CFDD153 pKa = 5.77 CFLLWFGCDD162 pKa = 3.12 LTKK165 pKa = 10.7 TSLHH169 pKa = 5.46 AWKK172 pKa = 10.02 HH173 pKa = 4.04 VMYY176 pKa = 10.96 NLDD179 pKa = 4.34 LDD181 pKa = 4.66 LLMFKK186 pKa = 10.21 QLNLVSFSFF195 pKa = 3.98
Molecular weight: 22.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.393
IPC2_protein 7.6
IPC_protein 7.424
Toseland 6.927
ProMoST 8.009
Dawson 8.126
Bjellqvist 8.609
Wikipedia 8.024
Rodwell 8.141
Grimsley 6.912
Solomon 8.2
Lehninger 8.214
Nozaki 8.931
DTASelect 8.258
Thurlkill 8.317
EMBOSS 8.346
Sillero 8.697
Patrickios 4.037
IPC_peptide 8.185
IPC2_peptide 7.761
IPC2.peptide.svr19 7.723
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1753
195
659
350.6
39.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.362 ± 0.997
2.852 ± 0.9
5.077 ± 0.207
6.275 ± 0.891
3.936 ± 0.644
5.705 ± 0.632
1.483 ± 0.445
5.305 ± 1.044
4.735 ± 1.419
10.667 ± 0.802
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.567 ± 0.571
5.476 ± 0.644
6.218 ± 1.657
4.792 ± 0.455
4.963 ± 0.497
7.644 ± 0.835
6.218 ± 0.967
5.819 ± 0.553
1.597 ± 0.317
3.309 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here