Canis familiaris papillomavirus 16
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5KYB3|A0A0C5KYB3_9PAPI Minor capsid protein L2 OS=Canis familiaris papillomavirus 16 OX=1619253 GN=L2 PE=3 SV=1
MM1 pKa = 7.39 IGKK4 pKa = 9.23 DD5 pKa = 3.29 ATLKK9 pKa = 10.97 DD10 pKa = 3.57 IVLEE14 pKa = 4.12 EE15 pKa = 4.14 QPTPVDD21 pKa = 3.87 EE22 pKa = 4.5 LWCDD26 pKa = 3.46 EE27 pKa = 4.51 EE28 pKa = 5.02 LAQSDD33 pKa = 4.22 EE34 pKa = 4.43 EE35 pKa = 4.48 EE36 pKa = 4.33 EE37 pKa = 4.6 SGSQQTKK44 pKa = 8.56 PFKK47 pKa = 10.41 IQTYY51 pKa = 10.0 CGNCNRR57 pKa = 11.84 GIRR60 pKa = 11.84 IVVLCTSEE68 pKa = 4.98 GIRR71 pKa = 11.84 TLQGLLCCNTSLCCPGCAASYY92 pKa = 10.01 RR93 pKa = 11.84 FEE95 pKa = 5.62 HH96 pKa = 6.78 GGG98 pKa = 3.29
Molecular weight: 10.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.276
IPC2_protein 4.507
IPC_protein 4.368
Toseland 4.215
ProMoST 4.457
Dawson 4.291
Bjellqvist 4.457
Wikipedia 4.151
Rodwell 4.202
Grimsley 4.126
Solomon 4.291
Lehninger 4.24
Nozaki 4.418
DTASelect 4.507
Thurlkill 4.215
EMBOSS 4.177
Sillero 4.469
Patrickios 0.693
IPC_peptide 4.304
IPC2_peptide 4.457
IPC2.peptide.svr19 4.411
Protein with the highest isoelectric point:
>tr|A0A0C5KYA9|A0A0C5KYA9_9PAPI Protein E7 OS=Canis familiaris papillomavirus 16 OX=1619253 GN=E7 PE=3 SV=1
MM1 pKa = 7.28 TLSPPLTLSPALHH14 pKa = 6.78 RR15 pKa = 11.84 PPPTLVRR22 pKa = 11.84 RR23 pKa = 11.84 LKK25 pKa = 10.91 DD26 pKa = 3.49 LLTGNQNGWPEE37 pKa = 4.2 EE38 pKa = 3.99 VEE40 pKa = 4.02 LQHH43 pKa = 6.69 IRR45 pKa = 11.84 RR46 pKa = 11.84 PGPKK50 pKa = 10.15 GQITPPASPPRR61 pKa = 11.84 PRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 HH66 pKa = 5.02 RR67 pKa = 11.84 SRR69 pKa = 11.84 RR70 pKa = 11.84 NRR72 pKa = 11.84 TPSPVFSWEE81 pKa = 3.95 DD82 pKa = 3.54 PYY84 pKa = 11.82 GEE86 pKa = 4.53 SQTPPPGSPLLTPHH100 pKa = 6.96 DD101 pKa = 3.53 RR102 pKa = 11.84 WSPQSQPTPLSPCPGASPLPLSTPEE127 pKa = 4.11 STHH130 pKa = 6.66 PPTPYY135 pKa = 9.99 QGLDD139 pKa = 3.22 LDD141 pKa = 4.73 LPRR144 pKa = 11.84 PLPRR148 pKa = 11.84 PTPLPLRR155 pKa = 11.84 DD156 pKa = 3.94 PRR158 pKa = 11.84 PSLSEE163 pKa = 3.51 QKK165 pKa = 10.44 KK166 pKa = 10.14 ALKK169 pKa = 9.82 QALDD173 pKa = 3.73 QVSKK177 pKa = 10.9 DD178 pKa = 3.23 AYY180 pKa = 10.79 LLRR183 pKa = 11.84 TEE185 pKa = 4.8 LLEE188 pKa = 5.12 DD189 pKa = 4.25 LDD191 pKa = 4.84 SFFAKK196 pKa = 10.54 LGIVPHH202 pKa = 6.56 LL203 pKa = 3.98
Molecular weight: 22.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.193
IPC2_protein 9.209
IPC_protein 9.487
Toseland 10.175
ProMoST 9.809
Dawson 10.321
Bjellqvist 9.984
Wikipedia 10.482
Rodwell 10.57
Grimsley 10.365
Solomon 10.379
Lehninger 10.365
Nozaki 10.16
DTASelect 9.97
Thurlkill 10.189
EMBOSS 10.57
Sillero 10.233
Patrickios 10.292
IPC_peptide 10.379
IPC2_peptide 8.653
IPC2.peptide.svr19 8.36
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2649
48
630
331.1
36.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.304 ± 0.443
2.039 ± 0.614
6.342 ± 0.537
5.512 ± 0.43
3.813 ± 0.589
7.173 ± 0.632
2.341 ± 0.196
3.926 ± 0.536
4.077 ± 0.723
8.758 ± 0.912
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.661 ± 0.32
3.624 ± 0.357
7.89 ± 1.398
4.002 ± 0.538
7.022 ± 0.438
7.55 ± 0.389
7.361 ± 0.307
6.644 ± 1.03
1.623 ± 0.312
2.341 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here