Micromonospora coxensis
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5889 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C5GWS8|A0A1C5GWS8_9ACTN Uncharacterized protein OS=Micromonospora coxensis OX=356852 GN=GA0070614_0491 PE=4 SV=1
MM1 pKa = 8.1 PYY3 pKa = 10.54 LPDD6 pKa = 3.55 LTTVLVDD13 pKa = 3.83 GARR16 pKa = 11.84 ITQGGVEE23 pKa = 4.22 YY24 pKa = 10.49 VIKK27 pKa = 10.7 ARR29 pKa = 11.84 GVGMVTLPSGQVVGCDD45 pKa = 3.44 PLIAPDD51 pKa = 3.51 TAAPFTVTVEE61 pKa = 3.98 PGRR64 pKa = 11.84 YY65 pKa = 9.3 RR66 pKa = 11.84 LDD68 pKa = 2.88 AWVAAVHH75 pKa = 5.56 QSGSEE80 pKa = 4.01 PQDD83 pKa = 2.89 RR84 pKa = 11.84 TAALQLVISDD94 pKa = 3.76 QPTMRR99 pKa = 11.84 WEE101 pKa = 3.95 LALTNGQDD109 pKa = 3.69 PTEE112 pKa = 4.44 LGADD116 pKa = 3.66 GFFGYY121 pKa = 9.69 PVDD124 pKa = 4.28 AGVGTLADD132 pKa = 3.71 AVAVGALAHH141 pKa = 6.52 WEE143 pKa = 3.86 FDD145 pKa = 3.43 QVDD148 pKa = 4.11 EE149 pKa = 4.49 VFIPAQVPPAPAAVDD164 pKa = 4.83 AITDD168 pKa = 3.72 EE169 pKa = 4.48 ASGANVIIVSSGWGDD184 pKa = 3.85 GIYY187 pKa = 8.61 PTFIGYY193 pKa = 9.31 AADD196 pKa = 4.2 GEE198 pKa = 4.7 VTSYY202 pKa = 9.43 VTDD205 pKa = 3.85 FLVIPNQTAA214 pKa = 2.68
Molecular weight: 22.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.732
IPC_protein 3.732
Toseland 3.503
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.101
Thurlkill 3.567
EMBOSS 3.694
Sillero 3.859
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A1C5II24|A0A1C5II24_9ACTN Uncharacterized protein OS=Micromonospora coxensis OX=356852 GN=GA0070614_2798 PE=4 SV=1
MM1 pKa = 7.28 GSVVKK6 pKa = 10.49 KK7 pKa = 9.45 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.48 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.7 LLRR22 pKa = 11.84 KK23 pKa = 7.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 LGKK33 pKa = 10.04
Molecular weight: 4.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5889
0
5889
1993409
29
9877
338.5
36.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.356 ± 0.049
0.742 ± 0.008
6.171 ± 0.026
4.982 ± 0.029
2.568 ± 0.017
9.55 ± 0.035
2.143 ± 0.015
2.868 ± 0.019
1.535 ± 0.02
10.538 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.536 ± 0.012
1.632 ± 0.02
6.546 ± 0.033
2.596 ± 0.018
8.634 ± 0.038
4.474 ± 0.022
6.183 ± 0.027
9.311 ± 0.032
1.618 ± 0.014
2.016 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here