Hubei Wuhan insect virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KK32|A0A1L3KK32_9VIRU RdRp catalytic domain-containing protein OS=Hubei Wuhan insect virus 9 OX=1922833 PE=4 SV=1
MM1 pKa = 7.71DD2 pKa = 3.35VWAKK6 pKa = 10.73AKK8 pKa = 10.35EE9 pKa = 4.09LFDD12 pKa = 3.55KK13 pKa = 10.81LVRR16 pKa = 11.84LVYY19 pKa = 10.72DD20 pKa = 3.87DD21 pKa = 4.62TVHH24 pKa = 7.65DD25 pKa = 5.45DD26 pKa = 3.29ISFTEE31 pKa = 5.16LDD33 pKa = 3.68LQDD36 pKa = 4.51WIDD39 pKa = 3.85TQDD42 pKa = 3.35SNIINLVKK50 pKa = 10.52NIHH53 pKa = 5.92YY54 pKa = 10.59NDD56 pKa = 4.15LKK58 pKa = 10.83VNSYY62 pKa = 11.24NLTIKK67 pKa = 10.62KK68 pKa = 9.77NSKK71 pKa = 9.21PALDD75 pKa = 3.51MSAVNTYY82 pKa = 11.19ASLQTVVFTPKK93 pKa = 10.58DD94 pKa = 3.98FNTLFCPMFKK104 pKa = 9.87IMKK107 pKa = 9.62KK108 pKa = 10.51RR109 pKa = 11.84MIDD112 pKa = 3.25TMNDD116 pKa = 2.85KK117 pKa = 11.07FIMYY121 pKa = 9.75TDD123 pKa = 3.92MPPSKK128 pKa = 10.28FSEE131 pKa = 4.18LLTKK135 pKa = 10.5KK136 pKa = 10.47FSTYY140 pKa = 9.71ILKK143 pKa = 10.09EE144 pKa = 3.9YY145 pKa = 10.47HH146 pKa = 6.63SLEE149 pKa = 4.29FDD151 pKa = 3.28VSKK154 pKa = 11.13YY155 pKa = 10.84DD156 pKa = 3.28KK157 pKa = 10.95SQNLLHH163 pKa = 6.81LLVDD167 pKa = 4.07CMIMRR172 pKa = 11.84HH173 pKa = 5.88FGIPEE178 pKa = 4.14CFVTMWFNGHH188 pKa = 7.12CDD190 pKa = 3.27TKK192 pKa = 11.12LYY194 pKa = 11.08DD195 pKa = 3.89PGNRR199 pKa = 11.84FYY201 pKa = 11.4CDD203 pKa = 2.7VYY205 pKa = 10.35FQRR208 pKa = 11.84KK209 pKa = 9.41SGDD212 pKa = 3.27PSTWLLNTQQILTMIVNCIPDD233 pKa = 3.83SAWAQIILVVASGDD247 pKa = 3.45DD248 pKa = 3.7SEE250 pKa = 5.07IFSTGKK256 pKa = 10.16LQICTSRR263 pKa = 11.84FSNVFNYY270 pKa = 8.63EE271 pKa = 3.7VKK273 pKa = 10.84VYY275 pKa = 11.16DD276 pKa = 4.78KK277 pKa = 9.4FTSMYY282 pKa = 9.21FCSKK286 pKa = 10.19FLIVTEE292 pKa = 4.26YY293 pKa = 8.54GTYY296 pKa = 9.36MLPDD300 pKa = 3.52IYY302 pKa = 11.3KK303 pKa = 10.47LIKK306 pKa = 10.12KK307 pKa = 10.35LGRR310 pKa = 11.84HH311 pKa = 5.48DD312 pKa = 4.28LKK314 pKa = 11.4SRR316 pKa = 11.84DD317 pKa = 3.44HH318 pKa = 6.56VLSFRR323 pKa = 11.84NSVLDD328 pKa = 3.48SLKK331 pKa = 10.9DD332 pKa = 3.43FSVPHH337 pKa = 7.4DD338 pKa = 3.48ILIQYY343 pKa = 7.2EE344 pKa = 3.75KK345 pKa = 11.32SMYY348 pKa = 10.33DD349 pKa = 2.86RR350 pKa = 11.84YY351 pKa = 10.7KK352 pKa = 10.62FPGTISINTVYY363 pKa = 10.68QSLCKK368 pKa = 9.7LTYY371 pKa = 10.37DD372 pKa = 3.8SNAFEE377 pKa = 4.76NLYY380 pKa = 10.29IPNPDD385 pKa = 3.55YY386 pKa = 11.37YY387 pKa = 11.0SNKK390 pKa = 9.58YY391 pKa = 9.99GKK393 pKa = 10.66LSDD396 pKa = 3.7LL397 pKa = 4.28

Molecular weight: 46.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KK09|A0A1L3KK09_9VIRU Uncharacterized protein OS=Hubei Wuhan insect virus 9 OX=1922833 PE=4 SV=1
MM1 pKa = 7.77PNTRR5 pKa = 11.84KK6 pKa = 8.02TLVFTNKK13 pKa = 9.56NYY15 pKa = 10.78KK16 pKa = 9.32NNNVNTIQRR25 pKa = 11.84GRR27 pKa = 11.84KK28 pKa = 8.39FNKK31 pKa = 8.54QRR33 pKa = 11.84RR34 pKa = 11.84SRR36 pKa = 11.84SRR38 pKa = 11.84TPRR41 pKa = 11.84RR42 pKa = 11.84SISRR46 pKa = 11.84SRR48 pKa = 11.84SRR50 pKa = 11.84SRR52 pKa = 11.84TSIRR56 pKa = 11.84KK57 pKa = 8.08NIKK60 pKa = 9.4YY61 pKa = 10.52GPIDD65 pKa = 3.92GFLHH69 pKa = 6.94RR70 pKa = 11.84IWSKK74 pKa = 9.31STSLIIDD81 pKa = 4.79PYY83 pKa = 8.9TYY85 pKa = 9.89IGIIIFSILIYY96 pKa = 10.18IHH98 pKa = 6.08QFHH101 pKa = 7.24IDD103 pKa = 3.57DD104 pKa = 3.92SAVNSFIKK112 pKa = 9.46TVKK115 pKa = 10.57KK116 pKa = 10.47NDD118 pKa = 3.59SLKK121 pKa = 10.83PFADD125 pKa = 2.85WFEE128 pKa = 4.38NNINKK133 pKa = 8.82FFALIMVLYY142 pKa = 10.05QSLQISPKK150 pKa = 8.82YY151 pKa = 10.33RR152 pKa = 11.84YY153 pKa = 7.51FTTLISFMLILLLPTLNVATYY174 pKa = 11.35SMALFGSILYY184 pKa = 10.37HH185 pKa = 5.92KK186 pKa = 9.59MKK188 pKa = 10.92YY189 pKa = 10.54KK190 pKa = 9.16MDD192 pKa = 4.75KK193 pKa = 10.46IVILSIFLLAAIWFYY208 pKa = 11.84YY209 pKa = 9.37EE210 pKa = 6.14DD211 pKa = 3.73LMKK214 pKa = 11.01YY215 pKa = 9.57HH216 pKa = 7.01NKK218 pKa = 10.16NINPSVRR225 pKa = 11.84PKK227 pKa = 10.48RR228 pKa = 11.84DD229 pKa = 3.16ADD231 pKa = 3.88DD232 pKa = 4.38VIEE235 pKa = 4.14AA236 pKa = 4.4

Molecular weight: 27.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4076

224

2418

679.3

79.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.999 ± 0.362

1.742 ± 0.337

7.139 ± 0.906

3.729 ± 0.681

5.397 ± 0.79

2.92 ± 0.297

2.87 ± 0.199

8.415 ± 1.051

8.194 ± 0.519

7.728 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.582 ± 0.112

5.618 ± 0.218

3.263 ± 0.327

2.355 ± 0.097

3.606 ± 0.75

8.023 ± 0.477

7.041 ± 0.454

6.722 ± 0.543

0.711 ± 0.148

6.943 ± 0.428
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski