Gordonia phage Wizard

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wizardvirus; Gordonia virus Wizard

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160DCM5|A0A160DCM5_9CAUD Uncharacterized protein OS=Gordonia phage Wizard OX=1838083 GN=74 PE=4 SV=1
MM1 pKa = 7.26IRR3 pKa = 11.84KK4 pKa = 9.67LSTALAALAILAAPVAVAMANPTPAPAQVPAPCVIQLGNGGTAPCPPPIVSTDD57 pKa = 3.04GSSIGGGANTDD68 pKa = 3.75PVNMGDD74 pKa = 4.63LPRR77 pKa = 11.84TGPDD81 pKa = 3.25YY82 pKa = 11.07SYY84 pKa = 10.78EE85 pKa = 4.21APTWTPDD92 pKa = 3.15EE93 pKa = 4.69EE94 pKa = 4.7EE95 pKa = 5.07PDD97 pKa = 3.7TEE99 pKa = 4.46EE100 pKa = 3.96PTEE103 pKa = 4.21TEE105 pKa = 4.06EE106 pKa = 4.15PAPEE110 pKa = 4.82AEE112 pKa = 4.19

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166Y2H9|A0A166Y2H9_9CAUD HTH DNA binding domain protein OS=Gordonia phage Wizard OX=1838083 GN=48 PE=4 SV=1
MM1 pKa = 7.19VNNIVHH7 pKa = 5.34MTNMRR12 pKa = 11.84NTGKK16 pKa = 10.64AKK18 pKa = 10.09FVPASWQPLIAGFLRR33 pKa = 11.84HH34 pKa = 5.95LRR36 pKa = 11.84AAGQPDD42 pKa = 3.68TTINTRR48 pKa = 11.84RR49 pKa = 11.84QQIEE53 pKa = 3.81RR54 pKa = 11.84MARR57 pKa = 11.84ALEE60 pKa = 4.22GEE62 pKa = 4.21PAEE65 pKa = 4.35VTRR68 pKa = 11.84DD69 pKa = 3.58AITDD73 pKa = 3.16YY74 pKa = 11.03HH75 pKa = 7.2AAQTWALEE83 pKa = 3.96TRR85 pKa = 11.84RR86 pKa = 11.84GHH88 pKa = 6.45RR89 pKa = 11.84NAAVSFFAWAHH100 pKa = 5.07SCGHH104 pKa = 6.14IPEE107 pKa = 5.11NPAQHH112 pKa = 6.34LPIVKK117 pKa = 10.05SSVPAPRR124 pKa = 11.84PAPDD128 pKa = 2.72RR129 pKa = 11.84VVNVALKK136 pKa = 9.37TPEE139 pKa = 3.91KK140 pKa = 10.24RR141 pKa = 11.84IQLMLRR147 pKa = 11.84LSCEE151 pKa = 3.66LGLRR155 pKa = 11.84RR156 pKa = 11.84AEE158 pKa = 4.07VAVVSTTDD166 pKa = 3.16VEE168 pKa = 4.77DD169 pKa = 4.3GVGGAMLRR177 pKa = 11.84VHH179 pKa = 6.86GKK181 pKa = 8.84GGKK184 pKa = 9.04IRR186 pKa = 11.84VIPISDD192 pKa = 3.53EE193 pKa = 3.66LALRR197 pKa = 11.84IEE199 pKa = 5.04DD200 pKa = 3.63GAAGHH205 pKa = 6.29TPGAPRR211 pKa = 11.84TGYY214 pKa = 10.61LFPGNDD220 pKa = 3.86GGHH223 pKa = 7.34LSPRR227 pKa = 11.84WVGTLCAEE235 pKa = 4.6ALPGGWTMHH244 pKa = 5.65TLRR247 pKa = 11.84HH248 pKa = 5.69RR249 pKa = 11.84FATRR253 pKa = 11.84AYY255 pKa = 9.41RR256 pKa = 11.84GSRR259 pKa = 11.84NLRR262 pKa = 11.84AVQVLLGHH270 pKa = 7.11ASLATTQRR278 pKa = 11.84YY279 pKa = 5.49TAVDD283 pKa = 3.57EE284 pKa = 4.78DD285 pKa = 4.18EE286 pKa = 4.97VRR288 pKa = 11.84AAMNSAAA295 pKa = 4.64

Molecular weight: 32.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

18513

29

1843

208.0

22.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.37 ± 0.528

0.832 ± 0.134

7.163 ± 0.325

5.634 ± 0.343

2.485 ± 0.173

8.643 ± 0.484

2.15 ± 0.207

4.202 ± 0.203

2.825 ± 0.25

7.832 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.069 ± 0.129

2.793 ± 0.174

6.293 ± 0.34

3.354 ± 0.16

7.298 ± 0.415

5.153 ± 0.227

7.119 ± 0.335

7.562 ± 0.212

2.209 ± 0.183

2.015 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski