Amino acid dipepetide frequency for Streptococcus phage phi-SsuFJSM8_rum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.225AlaAla: 3.225 ± 0.915
0.391AlaCys: 0.391 ± 0.185
4.105AlaAsp: 4.105 ± 0.911
5.278AlaGlu: 5.278 ± 0.846
3.225AlaPhe: 3.225 ± 0.497
4.3AlaGly: 4.3 ± 0.742
0.489AlaHis: 0.489 ± 0.237
7.037AlaIle: 7.037 ± 0.978
5.962AlaLys: 5.962 ± 0.764
5.571AlaLeu: 5.571 ± 0.843
1.075AlaMet: 1.075 ± 0.312
2.834AlaAsn: 2.834 ± 0.408
1.368AlaPro: 1.368 ± 0.367
2.15AlaGln: 2.15 ± 0.595
2.15AlaArg: 2.15 ± 0.407
5.375AlaSer: 5.375 ± 1.029
5.278AlaThr: 5.278 ± 0.805
3.421AlaVal: 3.421 ± 0.613
0.977AlaTrp: 0.977 ± 0.251
2.541AlaTyr: 2.541 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.195CysAla: 0.195 ± 0.126
0.293CysCys: 0.293 ± 0.147
0.195CysAsp: 0.195 ± 0.146
0.586CysGlu: 0.586 ± 0.234
0.293CysPhe: 0.293 ± 0.157
0.586CysGly: 0.586 ± 0.195
0.195CysHis: 0.195 ± 0.148
0.489CysIle: 0.489 ± 0.219
0.782CysLys: 0.782 ± 0.466
0.293CysLeu: 0.293 ± 0.166
0.195CysMet: 0.195 ± 0.135
0.195CysAsn: 0.195 ± 0.223
0.293CysPro: 0.293 ± 0.188
0.684CysGln: 0.684 ± 0.333
0.782CysArg: 0.782 ± 0.329
0.586CysSer: 0.586 ± 0.347
0.098CysThr: 0.098 ± 0.096
0.586CysVal: 0.586 ± 0.287
0.0CysTrp: 0.0 ± 0.0
1.075CysTyr: 1.075 ± 0.327
0.0CysXaa: 0.0 ± 0.0
Asp
3.909AspAla: 3.909 ± 0.569
0.684AspCys: 0.684 ± 0.297
2.052AspAsp: 2.052 ± 0.4
4.691AspGlu: 4.691 ± 0.803
3.127AspPhe: 3.127 ± 0.424
5.668AspGly: 5.668 ± 0.85
0.684AspHis: 0.684 ± 0.237
3.127AspIle: 3.127 ± 0.455
4.593AspLys: 4.593 ± 0.626
4.496AspLeu: 4.496 ± 0.803
1.466AspMet: 1.466 ± 0.41
2.639AspAsn: 2.639 ± 0.53
1.466AspPro: 1.466 ± 0.337
1.564AspGln: 1.564 ± 0.352
2.737AspArg: 2.737 ± 0.706
4.496AspSer: 4.496 ± 0.617
2.932AspThr: 2.932 ± 0.378
3.714AspVal: 3.714 ± 0.432
0.782AspTrp: 0.782 ± 0.281
2.443AspTyr: 2.443 ± 0.53
0.0AspXaa: 0.0 ± 0.0
Glu
4.496GluAla: 4.496 ± 0.563
0.098GluCys: 0.098 ± 0.111
4.203GluAsp: 4.203 ± 0.705
6.744GluGlu: 6.744 ± 1.195
2.443GluPhe: 2.443 ± 0.682
4.593GluGly: 4.593 ± 0.655
1.173GluHis: 1.173 ± 0.406
5.375GluIle: 5.375 ± 0.667
6.353GluLys: 6.353 ± 1.163
8.991GluLeu: 8.991 ± 1.052
2.248GluMet: 2.248 ± 0.486
3.616GluAsn: 3.616 ± 0.673
1.173GluPro: 1.173 ± 0.36
4.398GluGln: 4.398 ± 0.656
3.03GluArg: 3.03 ± 0.447
4.593GluSer: 4.593 ± 0.68
5.082GluThr: 5.082 ± 0.703
4.398GluVal: 4.398 ± 0.638
0.684GluTrp: 0.684 ± 0.293
1.466GluTyr: 1.466 ± 0.47
0.0GluXaa: 0.0 ± 0.0
Phe
2.248PheAla: 2.248 ± 0.804
0.586PheCys: 0.586 ± 0.304
2.932PheAsp: 2.932 ± 0.556
3.421PheGlu: 3.421 ± 0.66
1.564PhePhe: 1.564 ± 0.397
3.127PheGly: 3.127 ± 0.516
0.782PheHis: 0.782 ± 0.215
2.932PheIle: 2.932 ± 0.548
2.541PheLys: 2.541 ± 0.589
3.714PheLeu: 3.714 ± 0.75
1.075PheMet: 1.075 ± 0.318
2.248PheAsn: 2.248 ± 0.416
0.782PhePro: 0.782 ± 0.373
1.368PheGln: 1.368 ± 0.424
1.857PheArg: 1.857 ± 0.316
2.052PheSer: 2.052 ± 0.494
1.955PheThr: 1.955 ± 0.44
2.346PheVal: 2.346 ± 0.559
0.782PheTrp: 0.782 ± 0.343
1.564PheTyr: 1.564 ± 0.431
0.0PheXaa: 0.0 ± 0.0
Gly
2.932GlyAla: 2.932 ± 0.583
0.391GlyCys: 0.391 ± 0.174
4.398GlyAsp: 4.398 ± 0.796
3.714GlyGlu: 3.714 ± 0.723
2.248GlyPhe: 2.248 ± 0.428
2.834GlyGly: 2.834 ± 0.575
1.661GlyHis: 1.661 ± 0.397
5.18GlyIle: 5.18 ± 0.689
4.203GlyLys: 4.203 ± 0.672
6.157GlyLeu: 6.157 ± 1.052
1.759GlyMet: 1.759 ± 0.463
3.518GlyAsn: 3.518 ± 0.491
0.391GlyPro: 0.391 ± 0.145
3.03GlyGln: 3.03 ± 0.626
3.616GlyArg: 3.616 ± 0.471
4.007GlySer: 4.007 ± 0.668
4.203GlyThr: 4.203 ± 0.642
4.3GlyVal: 4.3 ± 0.8
0.782GlyTrp: 0.782 ± 0.245
2.639GlyTyr: 2.639 ± 0.589
0.0GlyXaa: 0.0 ± 0.0
His
0.782HisAla: 0.782 ± 0.234
0.098HisCys: 0.098 ± 0.103
1.173HisAsp: 1.173 ± 0.416
0.782HisGlu: 0.782 ± 0.305
0.489HisPhe: 0.489 ± 0.206
1.271HisGly: 1.271 ± 0.334
0.391HisHis: 0.391 ± 0.248
1.271HisIle: 1.271 ± 0.295
0.88HisLys: 0.88 ± 0.39
1.955HisLeu: 1.955 ± 0.377
0.391HisMet: 0.391 ± 0.22
1.075HisAsn: 1.075 ± 0.289
0.977HisPro: 0.977 ± 0.316
1.075HisGln: 1.075 ± 0.313
1.173HisArg: 1.173 ± 0.38
0.782HisSer: 0.782 ± 0.278
1.368HisThr: 1.368 ± 0.398
0.977HisVal: 0.977 ± 0.363
0.195HisTrp: 0.195 ± 0.149
0.977HisTyr: 0.977 ± 0.392
0.0HisXaa: 0.0 ± 0.0
Ile
5.571IleAla: 5.571 ± 0.773
0.391IleCys: 0.391 ± 0.181
6.353IleAsp: 6.353 ± 0.868
4.105IleGlu: 4.105 ± 0.641
1.857IlePhe: 1.857 ± 0.506
5.571IleGly: 5.571 ± 0.637
1.564IleHis: 1.564 ± 0.385
2.932IleIle: 2.932 ± 0.753
5.571IleLys: 5.571 ± 0.714
5.571IleLeu: 5.571 ± 0.814
1.075IleMet: 1.075 ± 0.319
4.105IleAsn: 4.105 ± 0.469
2.052IlePro: 2.052 ± 0.347
3.225IleGln: 3.225 ± 0.43
3.421IleArg: 3.421 ± 0.454
5.473IleSer: 5.473 ± 1.085
5.18IleThr: 5.18 ± 0.924
3.714IleVal: 3.714 ± 0.707
1.271IleTrp: 1.271 ± 0.431
2.346IleTyr: 2.346 ± 0.483
0.0IleXaa: 0.0 ± 0.0
Lys
5.278LysAla: 5.278 ± 0.738
0.391LysCys: 0.391 ± 0.179
3.421LysAsp: 3.421 ± 0.638
4.887LysGlu: 4.887 ± 0.653
3.03LysPhe: 3.03 ± 0.629
3.812LysGly: 3.812 ± 0.596
1.271LysHis: 1.271 ± 0.367
5.473LysIle: 5.473 ± 0.784
4.105LysLys: 4.105 ± 0.76
5.668LysLeu: 5.668 ± 0.766
1.857LysMet: 1.857 ± 0.45
3.127LysAsn: 3.127 ± 0.607
1.955LysPro: 1.955 ± 0.392
3.909LysGln: 3.909 ± 0.74
3.812LysArg: 3.812 ± 0.663
4.887LysSer: 4.887 ± 0.842
3.714LysThr: 3.714 ± 0.674
4.3LysVal: 4.3 ± 0.761
1.173LysTrp: 1.173 ± 0.333
2.248LysTyr: 2.248 ± 0.687
0.0LysXaa: 0.0 ± 0.0
Leu
6.939LeuAla: 6.939 ± 1.179
0.586LeuCys: 0.586 ± 0.32
5.668LeuAsp: 5.668 ± 0.756
7.819LeuGlu: 7.819 ± 1.096
3.518LeuPhe: 3.518 ± 0.66
4.105LeuGly: 4.105 ± 0.566
1.661LeuHis: 1.661 ± 0.326
5.18LeuIle: 5.18 ± 0.557
6.255LeuLys: 6.255 ± 0.564
7.134LeuLeu: 7.134 ± 0.85
2.443LeuMet: 2.443 ± 0.489
4.593LeuAsn: 4.593 ± 0.483
2.737LeuPro: 2.737 ± 0.549
3.616LeuGln: 3.616 ± 0.671
3.323LeuArg: 3.323 ± 0.447
8.894LeuSer: 8.894 ± 0.74
7.134LeuThr: 7.134 ± 0.904
5.571LeuVal: 5.571 ± 0.644
0.293LeuTrp: 0.293 ± 0.164
3.812LeuTyr: 3.812 ± 0.889
0.0LeuXaa: 0.0 ± 0.0
Met
2.15MetAla: 2.15 ± 0.456
0.195MetCys: 0.195 ± 0.141
1.661MetAsp: 1.661 ± 0.498
1.271MetGlu: 1.271 ± 0.413
0.782MetPhe: 0.782 ± 0.301
1.173MetGly: 1.173 ± 0.481
0.098MetHis: 0.098 ± 0.104
1.955MetIle: 1.955 ± 0.437
1.271MetLys: 1.271 ± 0.264
1.466MetLeu: 1.466 ± 0.414
0.489MetMet: 0.489 ± 0.252
0.782MetAsn: 0.782 ± 0.297
0.586MetPro: 0.586 ± 0.208
0.586MetGln: 0.586 ± 0.268
1.271MetArg: 1.271 ± 0.332
1.564MetSer: 1.564 ± 0.451
1.955MetThr: 1.955 ± 0.391
1.857MetVal: 1.857 ± 0.401
0.195MetTrp: 0.195 ± 0.149
0.391MetTyr: 0.391 ± 0.176
0.0MetXaa: 0.0 ± 0.0
Asn
3.812AsnAla: 3.812 ± 0.717
0.293AsnCys: 0.293 ± 0.15
2.15AsnAsp: 2.15 ± 0.405
2.932AsnGlu: 2.932 ± 0.578
2.443AsnPhe: 2.443 ± 0.393
4.691AsnGly: 4.691 ± 0.691
1.075AsnHis: 1.075 ± 0.366
2.443AsnIle: 2.443 ± 0.292
2.346AsnLys: 2.346 ± 0.468
5.082AsnLeu: 5.082 ± 1.089
0.88AsnMet: 0.88 ± 0.319
1.857AsnAsn: 1.857 ± 0.432
2.541AsnPro: 2.541 ± 0.564
2.639AsnGln: 2.639 ± 0.453
2.639AsnArg: 2.639 ± 0.558
2.737AsnSer: 2.737 ± 0.35
2.443AsnThr: 2.443 ± 0.721
3.127AsnVal: 3.127 ± 0.667
1.173AsnTrp: 1.173 ± 0.3
1.466AsnTyr: 1.466 ± 0.377
0.0AsnXaa: 0.0 ± 0.0
Pro
1.466ProAla: 1.466 ± 0.392
0.293ProCys: 0.293 ± 0.155
1.368ProAsp: 1.368 ± 0.376
2.346ProGlu: 2.346 ± 0.57
1.466ProPhe: 1.466 ± 0.387
0.88ProGly: 0.88 ± 0.405
0.782ProHis: 0.782 ± 0.262
1.857ProIle: 1.857 ± 0.435
1.564ProLys: 1.564 ± 0.378
2.834ProLeu: 2.834 ± 0.48
0.293ProMet: 0.293 ± 0.165
1.075ProAsn: 1.075 ± 0.266
0.782ProPro: 0.782 ± 0.344
0.782ProGln: 0.782 ± 0.327
1.661ProArg: 1.661 ± 0.426
1.955ProSer: 1.955 ± 0.477
2.15ProThr: 2.15 ± 0.533
1.759ProVal: 1.759 ± 0.441
0.293ProTrp: 0.293 ± 0.179
0.977ProTyr: 0.977 ± 0.278
0.0ProXaa: 0.0 ± 0.0
Gln
4.984GlnAla: 4.984 ± 0.779
0.782GlnCys: 0.782 ± 0.315
1.955GlnAsp: 1.955 ± 0.368
3.616GlnGlu: 3.616 ± 0.388
1.759GlnPhe: 1.759 ± 0.441
1.661GlnGly: 1.661 ± 0.475
0.586GlnHis: 0.586 ± 0.166
3.812GlnIle: 3.812 ± 0.516
2.639GlnLys: 2.639 ± 0.599
4.593GlnLeu: 4.593 ± 0.678
0.977GlnMet: 0.977 ± 0.409
2.443GlnAsn: 2.443 ± 0.474
1.466GlnPro: 1.466 ± 0.446
1.759GlnGln: 1.759 ± 0.337
1.564GlnArg: 1.564 ± 0.434
2.737GlnSer: 2.737 ± 0.57
3.518GlnThr: 3.518 ± 1.156
3.616GlnVal: 3.616 ± 0.768
0.88GlnTrp: 0.88 ± 0.339
0.782GlnTyr: 0.782 ± 0.316
0.0GlnXaa: 0.0 ± 0.0
Arg
1.661ArgAla: 1.661 ± 0.42
0.782ArgCys: 0.782 ± 0.281
2.346ArgAsp: 2.346 ± 0.457
3.616ArgGlu: 3.616 ± 0.504
1.759ArgPhe: 1.759 ± 0.521
2.932ArgGly: 2.932 ± 0.442
1.075ArgHis: 1.075 ± 0.391
3.616ArgIle: 3.616 ± 0.694
3.225ArgLys: 3.225 ± 0.742
4.105ArgLeu: 4.105 ± 0.688
0.586ArgMet: 0.586 ± 0.222
2.248ArgAsn: 2.248 ± 0.514
1.955ArgPro: 1.955 ± 0.491
3.03ArgGln: 3.03 ± 0.545
1.955ArgArg: 1.955 ± 0.412
1.857ArgSer: 1.857 ± 0.36
3.616ArgThr: 3.616 ± 0.774
3.323ArgVal: 3.323 ± 0.571
0.977ArgTrp: 0.977 ± 0.294
1.466ArgTyr: 1.466 ± 0.518
0.0ArgXaa: 0.0 ± 0.0
Ser
4.398SerAla: 4.398 ± 1.028
0.586SerCys: 0.586 ± 0.263
3.714SerAsp: 3.714 ± 0.682
5.571SerGlu: 5.571 ± 1.061
2.932SerPhe: 2.932 ± 0.584
3.714SerGly: 3.714 ± 0.52
0.977SerHis: 0.977 ± 0.277
5.962SerIle: 5.962 ± 0.667
4.789SerLys: 4.789 ± 0.746
6.255SerLeu: 6.255 ± 0.803
1.368SerMet: 1.368 ± 0.287
3.812SerAsn: 3.812 ± 0.727
2.052SerPro: 2.052 ± 0.513
3.225SerGln: 3.225 ± 0.871
3.225SerArg: 3.225 ± 0.63
6.157SerSer: 6.157 ± 1.155
4.691SerThr: 4.691 ± 0.897
3.909SerVal: 3.909 ± 0.589
0.977SerTrp: 0.977 ± 0.281
2.737SerTyr: 2.737 ± 0.554
0.0SerXaa: 0.0 ± 0.0
Thr
5.668ThrAla: 5.668 ± 0.697
0.195ThrCys: 0.195 ± 0.145
2.932ThrAsp: 2.932 ± 0.529
4.398ThrGlu: 4.398 ± 0.583
2.15ThrPhe: 2.15 ± 0.657
4.789ThrGly: 4.789 ± 0.883
1.173ThrHis: 1.173 ± 0.295
5.082ThrIle: 5.082 ± 0.869
4.691ThrLys: 4.691 ± 0.597
6.353ThrLeu: 6.353 ± 0.673
1.271ThrMet: 1.271 ± 0.387
3.323ThrAsn: 3.323 ± 0.677
1.368ThrPro: 1.368 ± 0.489
2.834ThrGln: 2.834 ± 0.827
1.955ThrArg: 1.955 ± 0.6
5.766ThrSer: 5.766 ± 1.486
5.668ThrThr: 5.668 ± 0.859
5.473ThrVal: 5.473 ± 0.873
1.075ThrTrp: 1.075 ± 0.294
2.932ThrTyr: 2.932 ± 0.584
0.0ThrXaa: 0.0 ± 0.0
Val
4.3ValAla: 4.3 ± 0.688
0.586ValCys: 0.586 ± 0.274
3.616ValAsp: 3.616 ± 0.709
5.278ValGlu: 5.278 ± 0.823
2.443ValPhe: 2.443 ± 0.739
3.323ValGly: 3.323 ± 0.607
1.173ValHis: 1.173 ± 0.265
4.3ValIle: 4.3 ± 0.794
4.105ValLys: 4.105 ± 0.597
6.353ValLeu: 6.353 ± 0.929
1.368ValMet: 1.368 ± 0.383
1.955ValAsn: 1.955 ± 0.433
1.271ValPro: 1.271 ± 0.315
2.834ValGln: 2.834 ± 0.485
3.714ValArg: 3.714 ± 0.825
4.398ValSer: 4.398 ± 0.938
4.789ValThr: 4.789 ± 0.663
3.225ValVal: 3.225 ± 0.416
0.977ValTrp: 0.977 ± 0.309
2.248ValTyr: 2.248 ± 0.596
0.0ValXaa: 0.0 ± 0.0
Trp
0.88TrpAla: 0.88 ± 0.24
0.293TrpCys: 0.293 ± 0.174
0.391TrpAsp: 0.391 ± 0.143
1.466TrpGlu: 1.466 ± 0.286
0.977TrpPhe: 0.977 ± 0.334
0.586TrpGly: 0.586 ± 0.22
0.293TrpHis: 0.293 ± 0.148
0.684TrpIle: 0.684 ± 0.248
0.684TrpLys: 0.684 ± 0.338
1.173TrpLeu: 1.173 ± 0.286
0.391TrpMet: 0.391 ± 0.179
1.661TrpAsn: 1.661 ± 0.374
0.098TrpPro: 0.098 ± 0.08
0.977TrpGln: 0.977 ± 0.342
0.293TrpArg: 0.293 ± 0.172
0.88TrpSer: 0.88 ± 0.345
1.271TrpThr: 1.271 ± 0.313
0.88TrpVal: 0.88 ± 0.353
0.195TrpTrp: 0.195 ± 0.114
0.195TrpTyr: 0.195 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.857TyrAla: 1.857 ± 0.399
0.489TyrCys: 0.489 ± 0.229
2.639TyrAsp: 2.639 ± 0.65
2.639TyrGlu: 2.639 ± 0.607
1.368TyrPhe: 1.368 ± 0.47
2.346TyrGly: 2.346 ± 0.639
0.977TyrHis: 0.977 ± 0.363
2.639TyrIle: 2.639 ± 0.554
1.759TyrLys: 1.759 ± 0.388
3.518TyrLeu: 3.518 ± 0.662
0.489TyrMet: 0.489 ± 0.27
1.661TyrAsn: 1.661 ± 0.303
1.271TyrPro: 1.271 ± 0.288
2.443TyrGln: 2.443 ± 0.54
1.955TyrArg: 1.955 ± 0.377
1.857TyrSer: 1.857 ± 0.537
2.052TyrThr: 2.052 ± 0.408
1.759TyrVal: 1.759 ± 0.427
0.586TyrTrp: 0.586 ± 0.213
1.271TyrTyr: 1.271 ± 0.396
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 40 proteins (10233 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski