Candidatus Profftella armatura
Average proteome isoelectric point is 8.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 372 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5R422|S5R422_9PROT tRNA(Ile)-lysidine synthase OS=Candidatus Profftella armatura OX=669502 GN=mesJ PE=3 SV=1
MM1 pKa = 7.54 SLTVLDD7 pKa = 4.29 IEE9 pKa = 5.16 SIANLSYY16 pKa = 11.09 LYY18 pKa = 9.89 IDD20 pKa = 3.86 KK21 pKa = 10.82 NQVYY25 pKa = 10.63 DD26 pKa = 3.53 ILEE29 pKa = 4.5 KK30 pKa = 10.69 INAIFSLIKK39 pKa = 10.11 SIKK42 pKa = 10.67 SIDD45 pKa = 3.56 TTNIEE50 pKa = 3.91 PLYY53 pKa = 10.31 YY54 pKa = 8.35 PTSLIQEE61 pKa = 3.94 KK62 pKa = 9.77 MLFLRR67 pKa = 11.84 EE68 pKa = 5.33 DD69 pKa = 2.82 ITTQNNYY76 pKa = 9.82 FEE78 pKa = 5.49 NYY80 pKa = 9.17 QNLAPIIHH88 pKa = 7.48 DD89 pKa = 3.58 NLYY92 pKa = 10.03 IVPKK96 pKa = 10.43 IFF98 pKa = 4.1
Molecular weight: 11.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.286
IPC2_protein 4.635
IPC_protein 4.469
Toseland 4.304
ProMoST 4.584
Dawson 4.418
Bjellqvist 4.571
Wikipedia 4.317
Rodwell 4.304
Grimsley 4.215
Solomon 4.418
Lehninger 4.368
Nozaki 4.546
DTASelect 4.698
Thurlkill 4.329
EMBOSS 4.329
Sillero 4.584
Patrickios 2.015
IPC_peptide 4.418
IPC2_peptide 4.571
IPC2.peptide.svr19 4.516
Protein with the highest isoelectric point:
>tr|S5RPH0|S5RPH0_9PROT Citrate synthase OS=Candidatus Profftella armatura OX=669502 GN=gltA PE=3 SV=1
MM1 pKa = 7.36 FKK3 pKa = 10.4 IKK5 pKa = 9.62 TKK7 pKa = 10.69 SSAKK11 pKa = 9.55 KK12 pKa = 9.9 RR13 pKa = 11.84 FFIRR17 pKa = 11.84 TGGVIKK23 pKa = 10.61 RR24 pKa = 11.84 GQAFKK29 pKa = 11.02 RR30 pKa = 11.84 HH31 pKa = 5.67 ILTKK35 pKa = 10.45 KK36 pKa = 6.89 STKK39 pKa = 9.45 VKK41 pKa = 10.34 RR42 pKa = 11.84 KK43 pKa = 9.67 LRR45 pKa = 11.84 GLASVHH51 pKa = 6.14 KK52 pKa = 10.62 SNIASVRR59 pKa = 11.84 AMMPNSS65 pKa = 3.33
Molecular weight: 7.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.472
IPC2_protein 11.096
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.778
Grimsley 12.852
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.501
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.019
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
372
0
372
135952
33
9019
365.5
41.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.399 ± 0.11
1.205 ± 0.037
4.257 ± 0.08
5.132 ± 0.107
4.934 ± 0.1
5.352 ± 0.117
1.809 ± 0.043
12.568 ± 0.128
10.222 ± 0.155
9.927 ± 0.097
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.11 ± 0.064
8.07 ± 0.172
3.16 ± 0.061
2.724 ± 0.043
3.868 ± 0.139
7.002 ± 0.123
4.316 ± 0.058
4.112 ± 0.1
0.891 ± 0.046
3.941 ± 0.09
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here