Paracoccus saliphilus
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4382 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N7KPQ9|A0A1N7KPQ9_9RHOB Acyl-CoA dehydrogenase OS=Paracoccus saliphilus OX=405559 GN=SAMN05421772_102152 PE=3 SV=1
MM1 pKa = 7.25 NCIGAASEE9 pKa = 4.18 LTEE12 pKa = 3.99 GAVPRR17 pKa = 11.84 EE18 pKa = 4.67 VYY20 pKa = 10.42 VLPHH24 pKa = 6.46 RR25 pKa = 11.84 LITALCLSLSPAAALAHH42 pKa = 4.93 PHH44 pKa = 5.74 VYY46 pKa = 9.64 IDD48 pKa = 4.68 AGLTLILDD56 pKa = 4.02 DD57 pKa = 5.0 SGALTGFEE65 pKa = 4.56 VEE67 pKa = 3.7 WSYY70 pKa = 12.28 DD71 pKa = 3.42 EE72 pKa = 5.86 LYY74 pKa = 11.1 SLLIIEE80 pKa = 5.86 DD81 pKa = 3.63 MGLDD85 pKa = 3.5 QDD87 pKa = 4.0 GDD89 pKa = 4.03 GVLTEE94 pKa = 4.8 EE95 pKa = 4.5 EE96 pKa = 3.9 NALIQGFDD104 pKa = 3.84 SDD106 pKa = 4.51 WEE108 pKa = 4.33 PGFDD112 pKa = 3.14 GRR114 pKa = 11.84 LYY116 pKa = 10.26 PRR118 pKa = 11.84 VDD120 pKa = 3.07 GRR122 pKa = 11.84 PVVLEE127 pKa = 3.96 DD128 pKa = 3.51 VRR130 pKa = 11.84 DD131 pKa = 4.55 FTAEE135 pKa = 3.82 YY136 pKa = 10.23 RR137 pKa = 11.84 DD138 pKa = 3.32 GRR140 pKa = 11.84 LISRR144 pKa = 11.84 HH145 pKa = 5.22 LHH147 pKa = 5.91 PLAEE151 pKa = 4.69 PLPMEE156 pKa = 5.25 APLQVQVYY164 pKa = 9.86 DD165 pKa = 3.46 PEE167 pKa = 4.84 FYY169 pKa = 11.17 VDD171 pKa = 4.67 FSMPEE176 pKa = 3.72 VPVIEE181 pKa = 4.3 GRR183 pKa = 11.84 EE184 pKa = 3.97 DD185 pKa = 3.93 CEE187 pKa = 4.49 VEE189 pKa = 5.22 LIPGDD194 pKa = 3.82 PSAAPDD200 pKa = 3.74 AYY202 pKa = 10.53 RR203 pKa = 11.84 EE204 pKa = 4.33 AIEE207 pKa = 4.37 AVLGAGTDD215 pKa = 3.53 PAEE218 pKa = 4.44 ADD220 pKa = 3.81 VVTVDD225 pKa = 3.86 IGSAGADD232 pKa = 3.38 EE233 pKa = 5.05 ADD235 pKa = 3.51 IRR237 pKa = 11.84 CDD239 pKa = 3.47 PGQGGG244 pKa = 3.37
Molecular weight: 26.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.808
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.516
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.923
Patrickios 1.863
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|A0A1N7K9V0|A0A1N7K9V0_9RHOB Pyrroline-5-carboxylate reductase OS=Paracoccus saliphilus OX=405559 GN=proC PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.12 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.69 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4382
0
4382
1362584
25
1879
311.0
33.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.94 ± 0.044
0.874 ± 0.013
5.948 ± 0.033
6.196 ± 0.038
3.627 ± 0.023
8.777 ± 0.036
2.105 ± 0.018
5.562 ± 0.03
2.905 ± 0.024
10.107 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.881 ± 0.017
2.611 ± 0.019
5.219 ± 0.025
3.191 ± 0.021
7.031 ± 0.042
5.363 ± 0.026
5.215 ± 0.025
6.875 ± 0.029
1.428 ± 0.014
2.144 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here