Amino acid dipepetide frequency for Sphingomonas sp. IBVSS2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.085AlaAla: 21.085 ± 0.224	0.979AlaCys: 0.979 ± 0.032	7.491AlaAsp: 7.491 ± 0.08	7.945AlaGlu: 7.945 ± 0.119	4.505AlaPhe: 4.505 ± 0.065	13.022AlaGly: 13.022 ± 0.147	2.447AlaHis: 2.447 ± 0.046	7.013AlaIle: 7.013 ± 0.089	3.766AlaLys: 3.766 ± 0.063	14.79AlaLeu: 14.79 ± 0.151	3.876AlaMet: 3.876 ± 0.066	3.447AlaAsn: 3.447 ± 0.084	7.489AlaPro: 7.489 ± 0.112	4.567AlaGln: 4.567 ± 0.072	10.901AlaArg: 10.901 ± 0.134	6.625AlaSer: 6.625 ± 0.08	7.021AlaThr: 7.021 ± 0.112	8.74AlaVal: 8.74 ± 0.082	1.881AlaTrp: 1.881 ± 0.042	2.694AlaTyr: 2.694 ± 0.047	0.001AlaXaa: 0.001 ± 0.001
Cys	0.923CysAla: 0.923 ± 0.032	0.061CysCys: 0.061 ± 0.007	0.443CysAsp: 0.443 ± 0.02	0.331CysGlu: 0.331 ± 0.016	0.251CysPhe: 0.251 ± 0.012	0.759CysGly: 0.759 ± 0.027	0.17CysHis: 0.17 ± 0.012	0.314CysIle: 0.314 ± 0.018	0.154CysLys: 0.154 ± 0.011	0.611CysLeu: 0.611 ± 0.022	0.136CysMet: 0.136 ± 0.011	0.184CysAsn: 0.184 ± 0.013	0.365CysPro: 0.365 ± 0.017	0.147CysGln: 0.147 ± 0.01	0.52CysArg: 0.52 ± 0.018	0.352CysSer: 0.352 ± 0.017	0.373CysThr: 0.373 ± 0.02	0.465CysVal: 0.465 ± 0.02	0.097CysTrp: 0.097 ± 0.008	0.147CysTyr: 0.147 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.944AspAla: 7.944 ± 0.084	0.402AspCys: 0.402 ± 0.017	2.856AspAsp: 2.856 ± 0.051	2.911AspGlu: 2.911 ± 0.065	2.146AspPhe: 2.146 ± 0.039	5.544AspGly: 5.544 ± 0.076	1.093AspHis: 1.093 ± 0.032	2.622AspIle: 2.622 ± 0.06	1.667AspLys: 1.667 ± 0.039	5.566AspLeu: 5.566 ± 0.075	1.282AspMet: 1.282 ± 0.033	1.245AspAsn: 1.245 ± 0.033	3.91AspPro: 3.91 ± 0.067	1.75AspGln: 1.75 ± 0.039	4.643AspArg: 4.643 ± 0.063	2.203AspSer: 2.203 ± 0.043	2.547AspThr: 2.547 ± 0.052	3.689AspVal: 3.689 ± 0.058	1.073AspTrp: 1.073 ± 0.028	1.6AspTyr: 1.6 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	7.905GluAla: 7.905 ± 0.111	0.261GluCys: 0.261 ± 0.014	2.507GluAsp: 2.507 ± 0.054	2.677GluGlu: 2.677 ± 0.06	1.386GluPhe: 1.386 ± 0.034	4.454GluGly: 4.454 ± 0.07	1.04GluHis: 1.04 ± 0.032	2.841GluIle: 2.841 ± 0.054	1.814GluLys: 1.814 ± 0.044	4.769GluLeu: 4.769 ± 0.067	1.326GluMet: 1.326 ± 0.035	1.217GluAsn: 1.217 ± 0.029	2.463GluPro: 2.463 ± 0.055	1.849GluGln: 1.849 ± 0.039	4.743GluArg: 4.743 ± 0.074	2.114GluSer: 2.114 ± 0.043	3.049GluThr: 3.049 ± 0.054	3.445GluVal: 3.445 ± 0.061	0.755GluTrp: 0.755 ± 0.025	0.978GluTyr: 0.978 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	4.991PheAla: 4.991 ± 0.065	0.264PheCys: 0.264 ± 0.015	2.631PheAsp: 2.631 ± 0.046	2.04PheGlu: 2.04 ± 0.041	1.209PhePhe: 1.209 ± 0.038	3.761PheGly: 3.761 ± 0.058	0.72PheHis: 0.72 ± 0.025	1.169PheIle: 1.169 ± 0.029	0.834PheLys: 0.834 ± 0.027	3.133PheLeu: 3.133 ± 0.051	0.666PheMet: 0.666 ± 0.024	1.052PheAsn: 1.052 ± 0.033	1.522PhePro: 1.522 ± 0.041	0.955PheGln: 0.955 ± 0.027	2.36PheArg: 2.36 ± 0.043	1.904PheSer: 1.904 ± 0.045	2.089PheThr: 2.089 ± 0.046	2.714PheVal: 2.714 ± 0.053	0.52PheTrp: 0.52 ± 0.023	0.929PheTyr: 0.929 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	11.392GlyAla: 11.392 ± 0.185	0.774GlyCys: 0.774 ± 0.023	5.051GlyAsp: 5.051 ± 0.095	4.879GlyGlu: 4.879 ± 0.08	3.824GlyPhe: 3.824 ± 0.06	9.345GlyGly: 9.345 ± 0.323	1.844GlyHis: 1.844 ± 0.04	4.577GlyIle: 4.577 ± 0.072	3.371GlyLys: 3.371 ± 0.062	8.882GlyLeu: 8.882 ± 0.088	2.421GlyMet: 2.421 ± 0.044	2.749GlyAsn: 2.749 ± 0.111	3.915GlyPro: 3.915 ± 0.06	2.991GlyGln: 2.991 ± 0.058	6.829GlyArg: 6.829 ± 0.077	5.053GlySer: 5.053 ± 0.097	5.833GlyThr: 5.833 ± 0.188	6.408GlyVal: 6.408 ± 0.077	1.809GlyTrp: 1.809 ± 0.045	2.512GlyTyr: 2.512 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	2.6HisAla: 2.6 ± 0.05	0.2HisCys: 0.2 ± 0.014	1.116HisAsp: 1.116 ± 0.033	0.892HisGlu: 0.892 ± 0.028	0.764HisPhe: 0.764 ± 0.023	2.062HisGly: 2.062 ± 0.04	0.52HisHis: 0.52 ± 0.021	0.832HisIle: 0.832 ± 0.025	0.377HisLys: 0.377 ± 0.017	1.779HisLeu: 1.779 ± 0.042	0.445HisMet: 0.445 ± 0.019	0.44HisAsn: 0.44 ± 0.017	1.206HisPro: 1.206 ± 0.031	0.504HisGln: 0.504 ± 0.023	1.45HisArg: 1.45 ± 0.039	0.824HisSer: 0.824 ± 0.025	0.624HisThr: 0.624 ± 0.023	1.437HisVal: 1.437 ± 0.032	0.363HisTrp: 0.363 ± 0.016	0.537HisTyr: 0.537 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.901IleAla: 7.901 ± 0.085	0.303IleCys: 0.303 ± 0.014	3.676IleAsp: 3.676 ± 0.05	3.314IleGlu: 3.314 ± 0.059	1.387IlePhe: 1.387 ± 0.034	5.206IleGly: 5.206 ± 0.081	0.786IleHis: 0.786 ± 0.022	1.376IleIle: 1.376 ± 0.036	1.048IleLys: 1.048 ± 0.036	4.054IleLeu: 4.054 ± 0.062	0.669IleMet: 0.669 ± 0.024	1.225IleAsn: 1.225 ± 0.042	2.135IlePro: 2.135 ± 0.043	1.194IleGln: 1.194 ± 0.03	3.178IleArg: 3.178 ± 0.054	2.297IleSer: 2.297 ± 0.059	2.478IleThr: 2.478 ± 0.077	3.967IleVal: 3.967 ± 0.053	0.575IleTrp: 0.575 ± 0.021	0.916IleTyr: 0.916 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	3.788LysAla: 3.788 ± 0.062	0.139LysCys: 0.139 ± 0.011	1.449LysAsp: 1.449 ± 0.037	1.053LysGlu: 1.053 ± 0.029	0.847LysPhe: 0.847 ± 0.024	2.604LysGly: 2.604 ± 0.054	0.497LysHis: 0.497 ± 0.02	1.309LysIle: 1.309 ± 0.034	0.917LysLys: 0.917 ± 0.031	3.239LysLeu: 3.239 ± 0.056	0.666LysMet: 0.666 ± 0.027	0.663LysAsn: 0.663 ± 0.024	1.973LysPro: 1.973 ± 0.045	0.853LysGln: 0.853 ± 0.026	2.101LysArg: 2.101 ± 0.047	1.325LysSer: 1.325 ± 0.037	1.52LysThr: 1.52 ± 0.038	2.006LysVal: 2.006 ± 0.045	0.403LysTrp: 0.403 ± 0.016	0.544LysTyr: 0.544 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	15.437LeuAla: 15.437 ± 0.162	0.725LeuCys: 0.725 ± 0.023	6.148LeuAsp: 6.148 ± 0.083	4.453LeuGlu: 4.453 ± 0.071	3.617LeuPhe: 3.617 ± 0.062	9.066LeuGly: 9.066 ± 0.1	1.824LeuHis: 1.824 ± 0.037	4.445LeuIle: 4.445 ± 0.066	2.836LeuLys: 2.836 ± 0.054	9.804LeuLeu: 9.804 ± 0.14	1.942LeuMet: 1.942 ± 0.042	2.457LeuAsn: 2.457 ± 0.056	5.787LeuPro: 5.787 ± 0.09	2.453LeuGln: 2.453 ± 0.045	7.035LeuArg: 7.035 ± 0.088	5.528LeuSer: 5.528 ± 0.073	5.268LeuThr: 5.268 ± 0.112	7.389LeuVal: 7.389 ± 0.09	1.295LeuTrp: 1.295 ± 0.038	2.01LeuTyr: 2.01 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	3.161MetAla: 3.161 ± 0.053	0.124MetCys: 0.124 ± 0.009	1.023MetAsp: 1.023 ± 0.028	0.933MetGlu: 0.933 ± 0.028	0.692MetPhe: 0.692 ± 0.023	1.81MetGly: 1.81 ± 0.038	0.447MetHis: 0.447 ± 0.022	1.291MetIle: 1.291 ± 0.035	0.815MetLys: 0.815 ± 0.028	2.652MetLeu: 2.652 ± 0.051	0.535MetMet: 0.535 ± 0.025	0.57MetAsn: 0.57 ± 0.018	1.479MetPro: 1.479 ± 0.034	0.689MetGln: 0.689 ± 0.023	1.867MetArg: 1.867 ± 0.04	1.214MetSer: 1.214 ± 0.033	1.535MetThr: 1.535 ± 0.039	1.492MetVal: 1.492 ± 0.035	0.191MetTrp: 0.191 ± 0.013	0.256MetTyr: 0.256 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.456AsnAla: 3.456 ± 0.094	0.223AsnCys: 0.223 ± 0.015	1.329AsnAsp: 1.329 ± 0.035	0.98AsnGlu: 0.98 ± 0.029	0.994AsnPhe: 0.994 ± 0.034	2.758AsnGly: 2.758 ± 0.098	0.47AsnHis: 0.47 ± 0.019	1.224AsnIle: 1.224 ± 0.045	0.598AsnLys: 0.598 ± 0.023	2.613AsnLeu: 2.613 ± 0.07	0.499AsnMet: 0.499 ± 0.02	0.768AsnAsn: 0.768 ± 0.048	1.802AsnPro: 1.802 ± 0.042	0.794AsnGln: 0.794 ± 0.032	1.804AsnArg: 1.804 ± 0.043	1.267AsnSer: 1.267 ± 0.049	1.242AsnThr: 1.242 ± 0.043	1.961AsnVal: 1.961 ± 0.062	0.425AsnTrp: 0.425 ± 0.019	0.804AsnTyr: 0.804 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	8.187ProAla: 8.187 ± 0.119	0.293ProCys: 0.293 ± 0.018	3.571ProAsp: 3.571 ± 0.059	3.65ProGlu: 3.65 ± 0.065	1.932ProPhe: 1.932 ± 0.041	5.708ProGly: 5.708 ± 0.096	1.034ProHis: 1.034 ± 0.032	2.412ProIle: 2.412 ± 0.046	1.345ProLys: 1.345 ± 0.039	5.022ProLeu: 5.022 ± 0.078	1.201ProMet: 1.201 ± 0.036	1.356ProAsn: 1.356 ± 0.037	2.865ProPro: 2.865 ± 0.079	1.662ProGln: 1.662 ± 0.039	3.472ProArg: 3.472 ± 0.064	2.565ProSer: 2.565 ± 0.05	2.338ProThr: 2.338 ± 0.051	4.384ProVal: 4.384 ± 0.072	0.786ProTrp: 0.786 ± 0.027	1.141ProTyr: 1.141 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	4.394GlnAla: 4.394 ± 0.063	0.181GlnCys: 0.181 ± 0.012	1.408GlnAsp: 1.408 ± 0.035	1.162GlnGlu: 1.162 ± 0.033	1.067GlnPhe: 1.067 ± 0.031	2.741GlnGly: 2.741 ± 0.052	0.53GlnHis: 0.53 ± 0.021	1.533GlnIle: 1.533 ± 0.04	0.889GlnLys: 0.889 ± 0.028	3.155GlnLeu: 3.155 ± 0.052	0.774GlnMet: 0.774 ± 0.021	0.733GlnAsn: 0.733 ± 0.026	1.766GlnPro: 1.766 ± 0.044	1.129GlnGln: 1.129 ± 0.029	2.44GlnArg: 2.44 ± 0.051	1.568GlnSer: 1.568 ± 0.049	1.542GlnThr: 1.542 ± 0.041	2.392GlnVal: 2.392 ± 0.043	0.418GlnTrp: 0.418 ± 0.018	0.66GlnTyr: 0.66 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	9.762ArgAla: 9.762 ± 0.13	0.453ArgCys: 0.453 ± 0.02	4.146ArgAsp: 4.146 ± 0.055	3.958ArgGlu: 3.958 ± 0.071	3.346ArgPhe: 3.346 ± 0.047	5.613ArgGly: 5.613 ± 0.075	1.64ArgHis: 1.64 ± 0.039	4.32ArgIle: 4.32 ± 0.055	2.03ArgLys: 2.03 ± 0.043	8.077ArgLeu: 8.077 ± 0.092	1.979ArgMet: 1.979 ± 0.039	1.868ArgAsn: 1.868 ± 0.048	4.0ArgPro: 4.0 ± 0.077	2.394ArgGln: 2.394 ± 0.047	5.992ArgArg: 5.992 ± 0.096	3.412ArgSer: 3.412 ± 0.05	3.744ArgThr: 3.744 ± 0.061	5.016ArgVal: 5.016 ± 0.075	1.284ArgTrp: 1.284 ± 0.034	1.883ArgTyr: 1.883 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	6.3SerAla: 6.3 ± 0.079	0.34SerCys: 0.34 ± 0.019	2.678SerAsp: 2.678 ± 0.046	2.227SerGlu: 2.227 ± 0.048	2.194SerPhe: 2.194 ± 0.048	5.557SerGly: 5.557 ± 0.131	0.858SerHis: 0.858 ± 0.029	2.529SerIle: 2.529 ± 0.055	1.239SerLys: 1.239 ± 0.033	4.773SerLeu: 4.773 ± 0.066	1.042SerMet: 1.042 ± 0.03	1.411SerAsn: 1.411 ± 0.041	2.765SerPro: 2.765 ± 0.05	1.498SerGln: 1.498 ± 0.038	3.263SerArg: 3.263 ± 0.054	2.529SerSer: 2.529 ± 0.063	2.722SerThr: 2.722 ± 0.053	3.485SerVal: 3.485 ± 0.059	0.855SerTrp: 0.855 ± 0.024	1.377SerTyr: 1.377 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	6.636ThrAla: 6.636 ± 0.121	0.316ThrCys: 0.316 ± 0.018	2.748ThrAsp: 2.748 ± 0.047	2.213ThrGlu: 2.213 ± 0.045	1.623ThrPhe: 1.623 ± 0.039	5.941ThrGly: 5.941 ± 0.169	0.915ThrHis: 0.915 ± 0.028	3.05ThrIle: 3.05 ± 0.071	1.102ThrLys: 1.102 ± 0.033	6.003ThrLeu: 6.003 ± 0.119	1.127ThrMet: 1.127 ± 0.03	1.44ThrAsn: 1.44 ± 0.066	3.555ThrPro: 3.555 ± 0.064	1.512ThrGln: 1.512 ± 0.042	3.658ThrArg: 3.658 ± 0.053	2.758ThrSer: 2.758 ± 0.071	2.842ThrThr: 2.842 ± 0.075	4.311ThrVal: 4.311 ± 0.102	0.634ThrTrp: 0.634 ± 0.023	1.213ThrTyr: 1.213 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	9.97ValAla: 9.97 ± 0.098	0.451ValCys: 0.451 ± 0.02	4.042ValAsp: 4.042 ± 0.057	4.285ValGlu: 4.285 ± 0.07	2.234ValPhe: 2.234 ± 0.049	5.241ValGly: 5.241 ± 0.079	1.342ValHis: 1.342 ± 0.039	3.395ValIle: 3.395 ± 0.05	2.018ValLys: 2.018 ± 0.043	6.723ValLeu: 6.723 ± 0.082	1.447ValMet: 1.447 ± 0.037	2.007ValAsn: 2.007 ± 0.052	4.154ValPro: 4.154 ± 0.071	2.167ValGln: 2.167 ± 0.042	5.322ValArg: 5.322 ± 0.076	3.97ValSer: 3.97 ± 0.062	4.919ValThr: 4.919 ± 0.104	5.112ValVal: 5.112 ± 0.073	0.814ValTrp: 0.814 ± 0.027	1.298ValTyr: 1.298 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.556TrpAla: 1.556 ± 0.036	0.117TrpCys: 0.117 ± 0.01	0.72TrpAsp: 0.72 ± 0.023	0.638TrpGlu: 0.638 ± 0.025	0.6TrpPhe: 0.6 ± 0.023	1.057TrpGly: 1.057 ± 0.027	0.358TrpHis: 0.358 ± 0.019	0.704TrpIle: 0.704 ± 0.025	0.495TrpLys: 0.495 ± 0.02	1.759TrpLeu: 1.759 ± 0.039	0.378TrpMet: 0.378 ± 0.015	0.506TrpAsn: 0.506 ± 0.018	0.72TrpPro: 0.72 ± 0.028	0.641TrpGln: 0.641 ± 0.018	1.375TrpArg: 1.375 ± 0.032	0.867TrpSer: 0.867 ± 0.026	0.828TrpThr: 0.828 ± 0.026	0.849TrpVal: 0.849 ± 0.029	0.283TrpTrp: 0.283 ± 0.014	0.349TrpTyr: 0.349 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.896TyrAla: 2.896 ± 0.047	0.197TyrCys: 0.197 ± 0.013	1.659TyrAsp: 1.659 ± 0.075	1.051TyrGlu: 1.051 ± 0.03	0.846TyrPhe: 0.846 ± 0.026	2.21TyrGly: 2.21 ± 0.046	0.435TyrHis: 0.435 ± 0.018	0.729TyrIle: 0.729 ± 0.025	0.581TyrLys: 0.581 ± 0.026	2.117TyrLeu: 2.117 ± 0.036	0.362TyrMet: 0.362 ± 0.016	0.671TyrAsn: 0.671 ± 0.036	1.051TyrPro: 1.051 ± 0.028	0.735TyrGln: 0.735 ± 0.031	1.962TyrArg: 1.962 ± 0.044	1.271TyrSer: 1.271 ± 0.035	1.085TyrThr: 1.085 ± 0.036	1.651TyrVal: 1.651 ± 0.038	0.34TyrTrp: 0.34 ± 0.015	0.731TyrTyr: 0.731 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 3925 proteins (1295300 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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