Amino acid dipepetide frequency for Mycobacterium phage PopTart

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.733AlaAla: 13.733 ± 1.61
0.916AlaCys: 0.916 ± 0.206
7.553AlaAsp: 7.553 ± 0.713
7.954AlaGlu: 7.954 ± 0.742
2.918AlaPhe: 2.918 ± 0.427
9.899AlaGly: 9.899 ± 1.133
2.461AlaHis: 2.461 ± 0.435
4.234AlaIle: 4.234 ± 0.52
3.948AlaLys: 3.948 ± 0.465
8.412AlaLeu: 8.412 ± 0.801
2.518AlaMet: 2.518 ± 0.412
2.575AlaAsn: 2.575 ± 0.408
5.207AlaPro: 5.207 ± 0.691
3.605AlaGln: 3.605 ± 0.491
6.752AlaArg: 6.752 ± 0.689
5.15AlaSer: 5.15 ± 0.557
7.095AlaThr: 7.095 ± 0.688
7.382AlaVal: 7.382 ± 0.76
2.689AlaTrp: 2.689 ± 0.409
2.06AlaTyr: 2.06 ± 0.339
0.0AlaXaa: 0.0 ± 0.0
Cys
1.087CysAla: 1.087 ± 0.328
0.0CysCys: 0.0 ± 0.0
1.316CysAsp: 1.316 ± 0.326
0.629CysGlu: 0.629 ± 0.189
0.114CysPhe: 0.114 ± 0.079
1.373CysGly: 1.373 ± 0.345
0.172CysHis: 0.172 ± 0.102
0.458CysIle: 0.458 ± 0.191
0.515CysLys: 0.515 ± 0.201
0.515CysLeu: 0.515 ± 0.213
0.172CysMet: 0.172 ± 0.094
0.401CysAsn: 0.401 ± 0.162
1.373CysPro: 1.373 ± 0.339
0.401CysGln: 0.401 ± 0.148
0.687CysArg: 0.687 ± 0.216
0.458CysSer: 0.458 ± 0.169
0.572CysThr: 0.572 ± 0.173
0.687CysVal: 0.687 ± 0.187
0.286CysTrp: 0.286 ± 0.124
0.057CysTyr: 0.057 ± 0.059
0.0CysXaa: 0.0 ± 0.0
Asp
6.867AspAla: 6.867 ± 0.621
0.744AspCys: 0.744 ± 0.192
4.635AspAsp: 4.635 ± 0.489
3.319AspGlu: 3.319 ± 0.492
1.888AspPhe: 1.888 ± 0.298
6.523AspGly: 6.523 ± 0.613
1.373AspHis: 1.373 ± 0.25
2.174AspIle: 2.174 ± 0.405
1.602AspLys: 1.602 ± 0.243
5.608AspLeu: 5.608 ± 0.555
0.916AspMet: 0.916 ± 0.27
1.831AspAsn: 1.831 ± 0.408
5.093AspPro: 5.093 ± 0.641
2.403AspGln: 2.403 ± 0.315
5.15AspArg: 5.15 ± 0.63
3.834AspSer: 3.834 ± 0.587
4.463AspThr: 4.463 ± 0.526
4.234AspVal: 4.234 ± 0.563
1.373AspTrp: 1.373 ± 0.254
2.117AspTyr: 2.117 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
6.352GluAla: 6.352 ± 0.754
0.801GluCys: 0.801 ± 0.213
3.033GluAsp: 3.033 ± 0.423
2.976GluGlu: 2.976 ± 0.548
1.602GluPhe: 1.602 ± 0.333
3.319GluGly: 3.319 ± 0.434
1.431GluHis: 1.431 ± 0.304
2.003GluIle: 2.003 ± 0.292
2.003GluLys: 2.003 ± 0.287
5.264GluLeu: 5.264 ± 0.656
1.774GluMet: 1.774 ± 0.373
1.888GluAsn: 1.888 ± 0.349
2.976GluPro: 2.976 ± 0.506
2.747GluGln: 2.747 ± 0.414
4.692GluArg: 4.692 ± 0.557
2.804GluSer: 2.804 ± 0.459
4.578GluThr: 4.578 ± 0.602
4.52GluVal: 4.52 ± 0.574
1.431GluTrp: 1.431 ± 0.288
2.003GluTyr: 2.003 ± 0.328
0.0GluXaa: 0.0 ± 0.0
Phe
3.376PheAla: 3.376 ± 0.465
0.286PheCys: 0.286 ± 0.134
2.232PheAsp: 2.232 ± 0.445
1.545PheGlu: 1.545 ± 0.304
0.858PhePhe: 0.858 ± 0.256
3.147PheGly: 3.147 ± 0.662
0.229PheHis: 0.229 ± 0.117
1.373PheIle: 1.373 ± 0.329
1.202PheLys: 1.202 ± 0.254
1.488PheLeu: 1.488 ± 0.238
0.687PheMet: 0.687 ± 0.192
1.144PheAsn: 1.144 ± 0.293
1.602PhePro: 1.602 ± 0.296
1.202PheGln: 1.202 ± 0.282
1.602PheArg: 1.602 ± 0.254
1.717PheSer: 1.717 ± 0.363
2.346PheThr: 2.346 ± 0.365
2.003PheVal: 2.003 ± 0.333
0.572PheTrp: 0.572 ± 0.164
0.973PheTyr: 0.973 ± 0.279
0.0PheXaa: 0.0 ± 0.0
Gly
9.041GlyAla: 9.041 ± 1.047
1.087GlyCys: 1.087 ± 0.252
6.409GlyAsp: 6.409 ± 0.635
4.177GlyGlu: 4.177 ± 0.576
3.033GlyPhe: 3.033 ± 0.499
9.728GlyGly: 9.728 ± 1.584
1.831GlyHis: 1.831 ± 0.274
4.406GlyIle: 4.406 ± 0.578
2.632GlyLys: 2.632 ± 0.424
6.237GlyLeu: 6.237 ± 0.536
2.289GlyMet: 2.289 ± 0.415
2.632GlyAsn: 2.632 ± 0.344
4.52GlyPro: 4.52 ± 0.545
2.461GlyGln: 2.461 ± 0.501
4.692GlyArg: 4.692 ± 0.702
6.008GlySer: 6.008 ± 0.781
7.153GlyThr: 7.153 ± 0.805
5.493GlyVal: 5.493 ± 0.56
2.689GlyTrp: 2.689 ± 0.422
1.888GlyTyr: 1.888 ± 0.355
0.0GlyXaa: 0.0 ± 0.0
His
2.174HisAla: 2.174 ± 0.466
0.343HisCys: 0.343 ± 0.174
0.858HisAsp: 0.858 ± 0.188
1.202HisGlu: 1.202 ± 0.266
0.515HisPhe: 0.515 ± 0.14
1.659HisGly: 1.659 ± 0.279
1.087HisHis: 1.087 ± 0.27
1.602HisIle: 1.602 ± 0.315
0.801HisLys: 0.801 ± 0.237
1.316HisLeu: 1.316 ± 0.272
0.629HisMet: 0.629 ± 0.181
0.916HisAsn: 0.916 ± 0.23
1.602HisPro: 1.602 ± 0.342
0.629HisGln: 0.629 ± 0.21
1.774HisArg: 1.774 ± 0.326
0.801HisSer: 0.801 ± 0.189
1.717HisThr: 1.717 ± 0.385
1.602HisVal: 1.602 ± 0.344
0.515HisTrp: 0.515 ± 0.158
0.572HisTyr: 0.572 ± 0.203
0.0HisXaa: 0.0 ± 0.0
Ile
5.264IleAla: 5.264 ± 0.578
0.343IleCys: 0.343 ± 0.167
3.834IleAsp: 3.834 ± 0.501
3.491IleGlu: 3.491 ± 0.392
0.744IlePhe: 0.744 ± 0.258
4.12IleGly: 4.12 ± 0.434
1.545IleHis: 1.545 ± 0.347
1.373IleIle: 1.373 ± 0.28
0.801IleLys: 0.801 ± 0.211
2.003IleLeu: 2.003 ± 0.358
0.286IleMet: 0.286 ± 0.144
1.831IleAsn: 1.831 ± 0.298
3.09IlePro: 3.09 ± 0.329
1.259IleGln: 1.259 ± 0.236
2.747IleArg: 2.747 ± 0.475
2.232IleSer: 2.232 ± 0.45
3.662IleThr: 3.662 ± 0.518
3.262IleVal: 3.262 ± 0.387
0.858IleTrp: 0.858 ± 0.208
0.744IleTyr: 0.744 ± 0.202
0.0IleXaa: 0.0 ± 0.0
Lys
3.491LysAla: 3.491 ± 0.457
0.572LysCys: 0.572 ± 0.233
1.488LysAsp: 1.488 ± 0.349
1.774LysGlu: 1.774 ± 0.378
1.202LysPhe: 1.202 ± 0.224
2.575LysGly: 2.575 ± 0.369
1.144LysHis: 1.144 ± 0.282
0.916LysIle: 0.916 ± 0.249
1.316LysLys: 1.316 ± 0.434
2.346LysLeu: 2.346 ± 0.445
0.744LysMet: 0.744 ± 0.177
0.973LysAsn: 0.973 ± 0.24
2.461LysPro: 2.461 ± 0.458
1.602LysGln: 1.602 ± 0.271
2.06LysArg: 2.06 ± 0.34
2.06LysSer: 2.06 ± 0.337
2.117LysThr: 2.117 ± 0.381
2.346LysVal: 2.346 ± 0.466
0.858LysTrp: 0.858 ± 0.242
0.858LysTyr: 0.858 ± 0.219
0.0LysXaa: 0.0 ± 0.0
Leu
7.954LeuAla: 7.954 ± 0.981
0.629LeuCys: 0.629 ± 0.185
5.035LeuAsp: 5.035 ± 0.638
3.948LeuGlu: 3.948 ± 0.478
2.403LeuPhe: 2.403 ± 0.294
5.264LeuGly: 5.264 ± 0.606
0.916LeuHis: 0.916 ± 0.226
3.09LeuIle: 3.09 ± 0.437
2.003LeuLys: 2.003 ± 0.354
4.635LeuLeu: 4.635 ± 0.596
1.545LeuMet: 1.545 ± 0.253
2.976LeuAsn: 2.976 ± 0.441
6.123LeuPro: 6.123 ± 0.701
2.575LeuGln: 2.575 ± 0.415
5.207LeuArg: 5.207 ± 0.74
5.093LeuSer: 5.093 ± 0.528
5.093LeuThr: 5.093 ± 0.518
4.463LeuVal: 4.463 ± 0.48
1.259LeuTrp: 1.259 ± 0.277
2.289LeuTyr: 2.289 ± 0.407
0.0LeuXaa: 0.0 ± 0.0
Met
2.289MetAla: 2.289 ± 0.388
0.0MetCys: 0.0 ± 0.0
1.144MetAsp: 1.144 ± 0.263
0.916MetGlu: 0.916 ± 0.21
0.687MetPhe: 0.687 ± 0.189
1.602MetGly: 1.602 ± 0.27
0.114MetHis: 0.114 ± 0.086
0.973MetIle: 0.973 ± 0.254
0.401MetLys: 0.401 ± 0.155
1.946MetLeu: 1.946 ± 0.335
0.629MetMet: 0.629 ± 0.264
0.973MetAsn: 0.973 ± 0.247
1.602MetPro: 1.602 ± 0.251
0.572MetGln: 0.572 ± 0.18
1.545MetArg: 1.545 ± 0.302
2.804MetSer: 2.804 ± 0.384
2.06MetThr: 2.06 ± 0.292
1.316MetVal: 1.316 ± 0.343
0.286MetTrp: 0.286 ± 0.127
0.343MetTyr: 0.343 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
3.491AsnAla: 3.491 ± 0.462
0.286AsnCys: 0.286 ± 0.123
1.946AsnAsp: 1.946 ± 0.35
1.545AsnGlu: 1.545 ± 0.33
0.801AsnPhe: 0.801 ± 0.265
3.834AsnGly: 3.834 ± 0.466
1.144AsnHis: 1.144 ± 0.243
1.831AsnIle: 1.831 ± 0.418
1.087AsnLys: 1.087 ± 0.269
2.289AsnLeu: 2.289 ± 0.443
0.687AsnMet: 0.687 ± 0.17
1.774AsnAsn: 1.774 ± 0.413
2.518AsnPro: 2.518 ± 0.402
1.087AsnGln: 1.087 ± 0.307
2.003AsnArg: 2.003 ± 0.363
1.488AsnSer: 1.488 ± 0.266
2.346AsnThr: 2.346 ± 0.292
1.888AsnVal: 1.888 ± 0.354
0.744AsnTrp: 0.744 ± 0.177
0.515AsnTyr: 0.515 ± 0.162
0.0AsnXaa: 0.0 ± 0.0
Pro
5.951ProAla: 5.951 ± 0.772
0.687ProCys: 0.687 ± 0.201
4.749ProAsp: 4.749 ± 0.535
4.177ProGlu: 4.177 ± 0.441
1.831ProPhe: 1.831 ± 0.318
6.924ProGly: 6.924 ± 0.691
1.431ProHis: 1.431 ± 0.295
2.689ProIle: 2.689 ± 0.347
2.174ProLys: 2.174 ± 0.366
4.635ProLeu: 4.635 ± 0.629
1.431ProMet: 1.431 ± 0.348
2.174ProAsn: 2.174 ± 0.38
3.891ProPro: 3.891 ± 0.563
2.174ProGln: 2.174 ± 0.421
3.433ProArg: 3.433 ± 0.51
3.491ProSer: 3.491 ± 0.52
3.605ProThr: 3.605 ± 0.468
4.635ProVal: 4.635 ± 0.541
1.144ProTrp: 1.144 ± 0.286
1.717ProTyr: 1.717 ± 0.323
0.0ProXaa: 0.0 ± 0.0
Gln
4.52GlnAla: 4.52 ± 0.571
0.172GlnCys: 0.172 ± 0.094
1.602GlnAsp: 1.602 ± 0.284
1.431GlnGlu: 1.431 ± 0.325
1.087GlnPhe: 1.087 ± 0.239
2.804GlnGly: 2.804 ± 0.448
0.916GlnHis: 0.916 ± 0.275
1.831GlnIle: 1.831 ± 0.32
1.373GlnLys: 1.373 ± 0.254
3.033GlnLeu: 3.033 ± 0.407
0.572GlnMet: 0.572 ± 0.205
0.801GlnAsn: 0.801 ± 0.234
2.689GlnPro: 2.689 ± 0.375
1.202GlnGln: 1.202 ± 0.325
2.632GlnArg: 2.632 ± 0.39
2.747GlnSer: 2.747 ± 0.379
1.774GlnThr: 1.774 ± 0.338
2.174GlnVal: 2.174 ± 0.349
0.687GlnTrp: 0.687 ± 0.238
0.801GlnTyr: 0.801 ± 0.198
0.0GlnXaa: 0.0 ± 0.0
Arg
6.294ArgAla: 6.294 ± 0.651
1.202ArgCys: 1.202 ± 0.301
4.177ArgAsp: 4.177 ± 0.525
4.978ArgGlu: 4.978 ± 0.662
1.831ArgPhe: 1.831 ± 0.385
4.005ArgGly: 4.005 ± 0.436
1.316ArgHis: 1.316 ± 0.382
3.548ArgIle: 3.548 ± 0.509
2.689ArgLys: 2.689 ± 0.429
5.093ArgLeu: 5.093 ± 0.576
2.117ArgMet: 2.117 ± 0.411
2.689ArgAsn: 2.689 ± 0.459
3.433ArgPro: 3.433 ± 0.453
1.946ArgGln: 1.946 ± 0.382
6.18ArgArg: 6.18 ± 0.943
3.09ArgSer: 3.09 ± 0.341
4.005ArgThr: 4.005 ± 0.561
4.978ArgVal: 4.978 ± 0.589
1.946ArgTrp: 1.946 ± 0.32
1.946ArgTyr: 1.946 ± 0.31
0.0ArgXaa: 0.0 ± 0.0
Ser
5.665SerAla: 5.665 ± 0.88
0.572SerCys: 0.572 ± 0.219
4.005SerAsp: 4.005 ± 0.485
3.033SerGlu: 3.033 ± 0.492
2.289SerPhe: 2.289 ± 0.429
6.466SerGly: 6.466 ± 0.689
0.801SerHis: 0.801 ± 0.213
2.804SerIle: 2.804 ± 0.418
2.346SerLys: 2.346 ± 0.393
4.063SerLeu: 4.063 ± 0.467
1.373SerMet: 1.373 ± 0.32
2.06SerAsn: 2.06 ± 0.394
3.433SerPro: 3.433 ± 0.391
1.888SerGln: 1.888 ± 0.347
3.834SerArg: 3.834 ± 0.454
3.719SerSer: 3.719 ± 0.589
3.147SerThr: 3.147 ± 0.436
4.234SerVal: 4.234 ± 0.522
1.259SerTrp: 1.259 ± 0.224
1.373SerTyr: 1.373 ± 0.205
0.0SerXaa: 0.0 ± 0.0
Thr
7.382ThrAla: 7.382 ± 0.679
0.801ThrCys: 0.801 ± 0.209
3.376ThrAsp: 3.376 ± 0.527
3.777ThrGlu: 3.777 ± 0.392
2.06ThrPhe: 2.06 ± 0.323
6.466ThrGly: 6.466 ± 0.634
1.659ThrHis: 1.659 ± 0.285
3.262ThrIle: 3.262 ± 0.495
2.06ThrLys: 2.06 ± 0.327
4.749ThrLeu: 4.749 ± 0.51
1.431ThrMet: 1.431 ± 0.29
2.518ThrAsn: 2.518 ± 0.392
4.978ThrPro: 4.978 ± 0.484
1.946ThrGln: 1.946 ± 0.313
4.635ThrArg: 4.635 ± 0.564
4.12ThrSer: 4.12 ± 0.471
5.665ThrThr: 5.665 ± 0.837
6.123ThrVal: 6.123 ± 0.556
1.144ThrTrp: 1.144 ± 0.302
1.488ThrTyr: 1.488 ± 0.346
0.0ThrXaa: 0.0 ± 0.0
Val
7.782ValAla: 7.782 ± 0.636
1.144ValCys: 1.144 ± 0.244
5.322ValAsp: 5.322 ± 0.684
4.578ValGlu: 4.578 ± 0.6
2.289ValPhe: 2.289 ± 0.331
5.436ValGly: 5.436 ± 0.593
1.545ValHis: 1.545 ± 0.313
2.747ValIle: 2.747 ± 0.456
2.403ValLys: 2.403 ± 0.341
5.379ValLeu: 5.379 ± 0.579
1.259ValMet: 1.259 ± 0.233
1.946ValAsn: 1.946 ± 0.32
4.12ValPro: 4.12 ± 0.428
3.09ValGln: 3.09 ± 0.413
4.005ValArg: 4.005 ± 0.54
4.635ValSer: 4.635 ± 0.517
4.921ValThr: 4.921 ± 0.573
6.466ValVal: 6.466 ± 0.872
1.717ValTrp: 1.717 ± 0.336
1.316ValTyr: 1.316 ± 0.255
0.0ValXaa: 0.0 ± 0.0
Trp
1.831TrpAla: 1.831 ± 0.309
0.401TrpCys: 0.401 ± 0.171
1.659TrpAsp: 1.659 ± 0.347
1.087TrpGlu: 1.087 ± 0.31
0.858TrpPhe: 0.858 ± 0.226
0.916TrpGly: 0.916 ± 0.196
0.858TrpHis: 0.858 ± 0.237
1.259TrpIle: 1.259 ± 0.292
0.801TrpLys: 0.801 ± 0.175
1.774TrpLeu: 1.774 ± 0.31
0.916TrpMet: 0.916 ± 0.262
0.458TrpAsn: 0.458 ± 0.199
1.03TrpPro: 1.03 ± 0.286
1.144TrpGln: 1.144 ± 0.276
2.003TrpArg: 2.003 ± 0.409
1.202TrpSer: 1.202 ± 0.298
1.831TrpThr: 1.831 ± 0.302
1.774TrpVal: 1.774 ± 0.444
0.973TrpTrp: 0.973 ± 0.214
0.343TrpTyr: 0.343 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.518TyrAla: 2.518 ± 0.437
0.286TyrCys: 0.286 ± 0.124
1.774TyrAsp: 1.774 ± 0.382
1.659TyrGlu: 1.659 ± 0.332
0.572TyrPhe: 0.572 ± 0.196
2.346TyrGly: 2.346 ± 0.523
0.343TyrHis: 0.343 ± 0.115
0.916TyrIle: 0.916 ± 0.192
0.744TyrLys: 0.744 ± 0.188
1.717TyrLeu: 1.717 ± 0.243
0.172TyrMet: 0.172 ± 0.102
0.744TyrAsn: 0.744 ± 0.225
1.202TyrPro: 1.202 ± 0.238
0.916TyrGln: 0.916 ± 0.221
1.888TyrArg: 1.888 ± 0.343
0.916TyrSer: 0.916 ± 0.238
1.488TyrThr: 1.488 ± 0.38
2.518TyrVal: 2.518 ± 0.378
0.687TyrTrp: 0.687 ± 0.201
0.744TyrTyr: 0.744 ± 0.198
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (17477 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski