Shewanella phage SppYZU05
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6JTQ1|A0A1W6JTQ1_9CAUD Chaperone of endosialidase OS=Shewanella phage SppYZU05 OX=1970795 GN=SppYZU05_69 PE=4 SV=1
MM1 pKa = 7.77 CNTSNLEE8 pKa = 4.31 TIMSQLNTIEE18 pKa = 4.18 TSSVQTLASTPSVNCWSEE36 pKa = 4.36 AEE38 pKa = 3.96 ISLVQYY44 pKa = 10.29 TNADD48 pKa = 2.97 WSILVQAVTNVASRR62 pKa = 11.84 GVMSEE67 pKa = 4.16 EE68 pKa = 4.2 ALCSIPTPAQDD79 pKa = 4.01 HH80 pKa = 6.9 CDD82 pKa = 3.89 SLHH85 pKa = 5.92 GALMLLATRR94 pKa = 11.84 EE95 pKa = 4.17 KK96 pKa = 10.8 FEE98 pKa = 4.07 QFQIDD103 pKa = 3.6 ALLDD107 pKa = 3.61 EE108 pKa = 5.12 VNFLVTGLTPSEE120 pKa = 4.12 LDD122 pKa = 4.09 EE123 pKa = 4.08 VWMAANGEE131 pKa = 4.32 EE132 pKa = 4.28 FF133 pKa = 3.7
Molecular weight: 14.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.884
IPC_protein 3.77
Toseland 3.605
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.63
Rodwell 3.617
Grimsley 3.528
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 3.961
Thurlkill 3.643
EMBOSS 3.643
Sillero 3.884
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.872
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A1W6JTK2|A0A1W6JTK2_9CAUD Uncharacterized protein OS=Shewanella phage SppYZU05 OX=1970795 GN=SppYZU05_19 PE=4 SV=1
MM1 pKa = 7.39 EE2 pKa = 5.21 NSMNSRR8 pKa = 11.84 QRR10 pKa = 11.84 SYY12 pKa = 10.77 YY13 pKa = 10.36 LRR15 pKa = 11.84 AKK17 pKa = 10.52 RR18 pKa = 11.84 IMKK21 pKa = 9.53 FLKK24 pKa = 9.55 TLPTLPTTATEE35 pKa = 4.69 WYY37 pKa = 6.71 PAHH40 pKa = 6.35 VRR42 pKa = 11.84 QARR45 pKa = 11.84 ARR47 pKa = 11.84 LKK49 pKa = 10.68 CLEE52 pKa = 4.04 SAQNCFVACGKK63 pKa = 9.51 NHH65 pKa = 6.82 DD66 pKa = 4.72 AEE68 pKa = 4.51 LLAPAIRR75 pKa = 11.84 NVTKK79 pKa = 10.62 RR80 pKa = 11.84 INIALEE86 pKa = 4.12 EE87 pKa = 3.88 IEE89 pKa = 4.05 IRR91 pKa = 11.84 KK92 pKa = 9.67 KK93 pKa = 10.37 RR94 pKa = 11.84 DD95 pKa = 2.92 AEE97 pKa = 4.05 MLHH100 pKa = 6.89 ARR102 pKa = 11.84 VQKK105 pKa = 10.0 VRR107 pKa = 11.84 LRR109 pKa = 11.84 RR110 pKa = 11.84 VPAQAGTVAFAGSGIDD126 pKa = 3.25 QNLFATIVV134 pKa = 3.09
Molecular weight: 15.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.589
IPC_protein 10.131
Toseland 10.672
ProMoST 10.277
Dawson 10.745
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 11.052
Grimsley 10.789
Solomon 10.847
Lehninger 10.818
Nozaki 10.657
DTASelect 10.409
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.789
IPC_peptide 10.847
IPC2_peptide 9.414
IPC2.peptide.svr19 8.632
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
16193
30
1251
234.7
26.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.794 ± 0.539
1.198 ± 0.151
5.879 ± 0.224
6.2 ± 0.281
3.39 ± 0.243
6.812 ± 0.349
2.137 ± 0.147
5.299 ± 0.213
5.021 ± 0.28
8.38 ± 0.275
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.705 ± 0.14
4.23 ± 0.23
4.459 ± 0.179
4.15 ± 0.199
5.811 ± 0.209
5.824 ± 0.203
6.528 ± 0.267
7.361 ± 0.336
1.464 ± 0.129
3.359 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here