Mycobacterium phage B1
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5XV90|A0A2Z5XV90_9CAUD Putative esterase/lipase OS=Mycobacterium phage B1 OX=2077133 PE=4 SV=1
MM1 pKa = 7.52 MLTQEE6 pKa = 4.41 DD7 pKa = 4.94 FEE9 pKa = 6.42 AEE11 pKa = 4.22 LVQDD15 pKa = 4.12 SYY17 pKa = 11.9 YY18 pKa = 11.25 DD19 pKa = 3.48 GMNMFEE25 pKa = 4.27 QAAQAGLEE33 pKa = 3.96 GRR35 pKa = 11.84 NYY37 pKa = 10.83 LL38 pKa = 4.3
Molecular weight: 4.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.963
IPC2_protein 3.668
IPC_protein 3.414
Toseland 3.287
ProMoST 3.656
Dawson 3.439
Bjellqvist 3.617
Wikipedia 3.414
Rodwell 3.3
Grimsley 3.236
Solomon 3.325
Lehninger 3.274
Nozaki 3.617
DTASelect 3.656
Thurlkill 3.401
EMBOSS 3.414
Sillero 3.554
Patrickios 0.299
IPC_peptide 3.325
IPC2_peptide 3.503
IPC2.peptide.svr19 3.64
Protein with the highest isoelectric point:
>tr|A0A2Z5XV84|A0A2Z5XV84_9CAUD Uncharacterized protein OS=Mycobacterium phage B1 OX=2077133 PE=4 SV=1
MM1 pKa = 6.94 IVARR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 VALGVASAGTVAVGGLAFALSFTALRR33 pKa = 11.84 DD34 pKa = 3.56 LSASNGVAQAWMVPLVVDD52 pKa = 3.79 GGIIVATAATVALRR66 pKa = 11.84 RR67 pKa = 11.84 HH68 pKa = 5.22 QWYY71 pKa = 9.42 AWTLLILSSLVSVAGNVAHH90 pKa = 6.57 AQAHH94 pKa = 5.49 GAIAMVIAAIPPLWLLAATHH114 pKa = 5.73 LTVMLSRR121 pKa = 11.84 SDD123 pKa = 3.85 KK124 pKa = 10.87 EE125 pKa = 4.24 PVPVAAEE132 pKa = 3.9 PLHH135 pKa = 6.32 IANAAA140 pKa = 3.29
Molecular weight: 14.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.589
IPC_protein 10.789
Toseland 10.774
ProMoST 10.877
Dawson 10.847
Bjellqvist 10.701
Wikipedia 11.184
Rodwell 10.73
Grimsley 10.906
Solomon 11.14
Lehninger 11.082
Nozaki 10.745
DTASelect 10.701
Thurlkill 10.774
EMBOSS 11.213
Sillero 10.804
Patrickios 10.657
IPC_peptide 11.14
IPC2_peptide 9.911
IPC2.peptide.svr19 8.737
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
13722
38
1014
188.0
20.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.678 ± 0.406
0.743 ± 0.11
6.391 ± 0.239
6.843 ± 0.376
3.345 ± 0.212
8.177 ± 0.53
2.092 ± 0.202
4.722 ± 0.158
4.307 ± 0.204
8.279 ± 0.291
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.463 ± 0.127
3.571 ± 0.227
5.764 ± 0.396
3.848 ± 0.342
6.69 ± 0.325
5.109 ± 0.244
5.728 ± 0.222
7.39 ± 0.236
1.946 ± 0.139
2.915 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here