Amino acid dipepetide frequency for Humibacter sp. WJ7-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.52AlaAla: 19.52 ± 0.182
0.748AlaCys: 0.748 ± 0.027
8.012AlaAsp: 8.012 ± 0.097
7.264AlaGlu: 7.264 ± 0.101
3.995AlaPhe: 3.995 ± 0.067
12.124AlaGly: 12.124 ± 0.131
2.579AlaHis: 2.579 ± 0.045
5.786AlaIle: 5.786 ± 0.079
2.957AlaLys: 2.957 ± 0.073
13.465AlaLeu: 13.465 ± 0.119
2.67AlaMet: 2.67 ± 0.052
2.48AlaAsn: 2.48 ± 0.055
6.025AlaPro: 6.025 ± 0.089
3.901AlaGln: 3.901 ± 0.066
8.906AlaArg: 8.906 ± 0.108
8.641AlaSer: 8.641 ± 0.102
7.732AlaThr: 7.732 ± 0.111
11.912AlaVal: 11.912 ± 0.13
1.869AlaTrp: 1.869 ± 0.046
2.476AlaTyr: 2.476 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.672CysAla: 0.672 ± 0.027
0.056CysCys: 0.056 ± 0.007
0.354CysAsp: 0.354 ± 0.019
0.218CysGlu: 0.218 ± 0.015
0.191CysPhe: 0.191 ± 0.013
0.628CysGly: 0.628 ± 0.026
0.111CysHis: 0.111 ± 0.01
0.205CysIle: 0.205 ± 0.012
0.072CysLys: 0.072 ± 0.008
0.513CysLeu: 0.513 ± 0.022
0.085CysMet: 0.085 ± 0.009
0.11CysAsn: 0.11 ± 0.009
0.283CysPro: 0.283 ± 0.017
0.129CysGln: 0.129 ± 0.01
0.336CysArg: 0.336 ± 0.019
0.432CysSer: 0.432 ± 0.019
0.404CysThr: 0.404 ± 0.026
0.502CysVal: 0.502 ± 0.023
0.084CysTrp: 0.084 ± 0.009
0.113CysTyr: 0.113 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
9.614AspAla: 9.614 ± 0.101
0.257AspCys: 0.257 ± 0.014
4.712AspAsp: 4.712 ± 0.082
4.281AspGlu: 4.281 ± 0.068
1.738AspPhe: 1.738 ± 0.04
6.173AspGly: 6.173 ± 0.091
1.238AspHis: 1.238 ± 0.036
2.247AspIle: 2.247 ± 0.045
1.046AspLys: 1.046 ± 0.034
5.807AspLeu: 5.807 ± 0.074
0.75AspMet: 0.75 ± 0.027
1.071AspAsn: 1.071 ± 0.04
3.78AspPro: 3.78 ± 0.064
1.572AspGln: 1.572 ± 0.044
4.199AspArg: 4.199 ± 0.065
2.906AspSer: 2.906 ± 0.058
3.056AspThr: 3.056 ± 0.057
5.475AspVal: 5.475 ± 0.073
0.943AspTrp: 0.943 ± 0.029
1.402AspTyr: 1.402 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
6.133GluAla: 6.133 ± 0.081
0.252GluCys: 0.252 ± 0.016
2.238GluAsp: 2.238 ± 0.049
2.331GluGlu: 2.331 ± 0.053
1.695GluPhe: 1.695 ± 0.043
3.383GluGly: 3.383 ± 0.054
1.708GluHis: 1.708 ± 0.036
2.227GluIle: 2.227 ± 0.047
1.291GluLys: 1.291 ± 0.038
6.292GluLeu: 6.292 ± 0.094
0.749GluMet: 0.749 ± 0.025
1.187GluAsn: 1.187 ± 0.031
2.862GluPro: 2.862 ± 0.047
2.195GluGln: 2.195 ± 0.045
5.067GluArg: 5.067 ± 0.096
2.923GluSer: 2.923 ± 0.052
2.887GluThr: 2.887 ± 0.054
4.004GluVal: 4.004 ± 0.065
0.823GluTrp: 0.823 ± 0.029
1.102GluTyr: 1.102 ± 0.034
0.0GluXaa: 0.0 ± 0.0
Phe
4.261PheAla: 4.261 ± 0.072
0.201PheCys: 0.201 ± 0.013
2.49PheAsp: 2.49 ± 0.046
1.662PheGlu: 1.662 ± 0.036
1.041PhePhe: 1.041 ± 0.041
3.545PheGly: 3.545 ± 0.064
0.588PheHis: 0.588 ± 0.026
1.17PheIle: 1.17 ± 0.037
0.526PheLys: 0.526 ± 0.021
2.596PheLeu: 2.596 ± 0.053
0.462PheMet: 0.462 ± 0.02
0.706PheAsn: 0.706 ± 0.025
1.311PhePro: 1.311 ± 0.034
0.755PheGln: 0.755 ± 0.026
1.777PheArg: 1.777 ± 0.038
1.865PheSer: 1.865 ± 0.039
2.123PheThr: 2.123 ± 0.048
3.001PheVal: 3.001 ± 0.057
0.522PheTrp: 0.522 ± 0.019
0.662PheTyr: 0.662 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
10.547GlyAla: 10.547 ± 0.137
0.625GlyCys: 0.625 ± 0.026
5.348GlyAsp: 5.348 ± 0.072
4.613GlyGlu: 4.613 ± 0.067
3.204GlyPhe: 3.204 ± 0.051
7.8GlyGly: 7.8 ± 0.165
1.963GlyHis: 1.963 ± 0.045
4.997GlyIle: 4.997 ± 0.082
2.193GlyLys: 2.193 ± 0.055
8.575GlyLeu: 8.575 ± 0.101
2.043GlyMet: 2.043 ± 0.045
1.804GlyAsn: 1.804 ± 0.045
3.664GlyPro: 3.664 ± 0.063
2.377GlyGln: 2.377 ± 0.051
6.114GlyArg: 6.114 ± 0.078
5.96GlySer: 5.96 ± 0.104
5.972GlyThr: 5.972 ± 0.101
8.031GlyVal: 8.031 ± 0.091
1.724GlyTrp: 1.724 ± 0.048
2.283GlyTyr: 2.283 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
2.504HisAla: 2.504 ± 0.051
0.128HisCys: 0.128 ± 0.011
1.597HisAsp: 1.597 ± 0.043
1.26HisGlu: 1.26 ± 0.029
0.577HisPhe: 0.577 ± 0.021
2.101HisGly: 2.101 ± 0.05
0.546HisHis: 0.546 ± 0.025
0.739HisIle: 0.739 ± 0.023
0.325HisLys: 0.325 ± 0.018
1.931HisLeu: 1.931 ± 0.045
0.292HisMet: 0.292 ± 0.015
0.39HisAsn: 0.39 ± 0.018
1.604HisPro: 1.604 ± 0.04
0.488HisGln: 0.488 ± 0.024
1.619HisArg: 1.619 ± 0.043
1.078HisSer: 1.078 ± 0.026
1.151HisThr: 1.151 ± 0.032
1.83HisVal: 1.83 ± 0.041
0.322HisTrp: 0.322 ± 0.018
0.476HisTyr: 0.476 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.93IleAla: 6.93 ± 0.085
0.243IleCys: 0.243 ± 0.016
3.339IleAsp: 3.339 ± 0.056
2.635IleGlu: 2.635 ± 0.055
1.076IlePhe: 1.076 ± 0.034
4.882IleGly: 4.882 ± 0.083
0.717IleHis: 0.717 ± 0.029
1.738IleIle: 1.738 ± 0.047
0.772IleLys: 0.772 ± 0.028
3.303IleLeu: 3.303 ± 0.061
0.614IleMet: 0.614 ± 0.021
0.881IleAsn: 0.881 ± 0.028
2.363IlePro: 2.363 ± 0.051
0.98IleGln: 0.98 ± 0.029
2.481IleArg: 2.481 ± 0.047
2.234IleSer: 2.234 ± 0.051
2.962IleThr: 2.962 ± 0.067
4.916IleVal: 4.916 ± 0.087
0.53IleTrp: 0.53 ± 0.024
0.761IleTyr: 0.761 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
2.867LysAla: 2.867 ± 0.063
0.065LysCys: 0.065 ± 0.008
1.157LysAsp: 1.157 ± 0.036
0.901LysGlu: 0.901 ± 0.03
0.503LysPhe: 0.503 ± 0.024
1.692LysGly: 1.692 ± 0.039
0.482LysHis: 0.482 ± 0.023
0.871LysIle: 0.871 ± 0.032
0.796LysLys: 0.796 ± 0.036
1.929LysLeu: 1.929 ± 0.046
0.35LysMet: 0.35 ± 0.018
0.602LysAsn: 0.602 ± 0.023
1.248LysPro: 1.248 ± 0.035
0.861LysGln: 0.861 ± 0.029
1.583LysArg: 1.583 ± 0.045
1.274LysSer: 1.274 ± 0.037
1.441LysThr: 1.441 ± 0.045
1.697LysVal: 1.697 ± 0.044
0.288LysTrp: 0.288 ± 0.016
0.517LysTyr: 0.517 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
13.92LeuAla: 13.92 ± 0.14
0.579LeuCys: 0.579 ± 0.022
6.557LeuAsp: 6.557 ± 0.084
4.695LeuGlu: 4.695 ± 0.076
2.986LeuPhe: 2.986 ± 0.055
9.058LeuGly: 9.058 ± 0.108
1.955LeuHis: 1.955 ± 0.042
4.435LeuIle: 4.435 ± 0.082
1.876LeuLys: 1.876 ± 0.046
9.783LeuLeu: 9.783 ± 0.139
1.63LeuMet: 1.63 ± 0.04
1.891LeuAsn: 1.891 ± 0.05
5.353LeuPro: 5.353 ± 0.07
2.51LeuGln: 2.51 ± 0.051
7.177LeuArg: 7.177 ± 0.105
5.83LeuSer: 5.83 ± 0.078
6.308LeuThr: 6.308 ± 0.077
9.074LeuVal: 9.074 ± 0.108
1.317LeuTrp: 1.317 ± 0.037
1.822LeuTyr: 1.822 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
2.092MetAla: 2.092 ± 0.045
0.108MetCys: 0.108 ± 0.01
0.716MetAsp: 0.716 ± 0.025
0.563MetGlu: 0.563 ± 0.024
0.511MetPhe: 0.511 ± 0.021
1.215MetGly: 1.215 ± 0.031
0.366MetHis: 0.366 ± 0.017
0.779MetIle: 0.779 ± 0.026
0.412MetLys: 0.412 ± 0.018
2.105MetLeu: 2.105 ± 0.043
0.336MetMet: 0.336 ± 0.021
0.518MetAsn: 0.518 ± 0.023
1.29MetPro: 1.29 ± 0.033
0.554MetGln: 0.554 ± 0.022
1.376MetArg: 1.376 ± 0.034
1.463MetSer: 1.463 ± 0.033
1.583MetThr: 1.583 ± 0.039
1.356MetVal: 1.356 ± 0.038
0.194MetTrp: 0.194 ± 0.011
0.268MetTyr: 0.268 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.776AsnAla: 2.776 ± 0.06
0.113AsnCys: 0.113 ± 0.01
1.365AsnAsp: 1.365 ± 0.039
1.008AsnGlu: 1.008 ± 0.027
0.597AsnPhe: 0.597 ± 0.025
2.341AsnGly: 2.341 ± 0.061
0.389AsnHis: 0.389 ± 0.02
0.857AsnIle: 0.857 ± 0.032
0.481AsnLys: 0.481 ± 0.02
1.897AsnLeu: 1.897 ± 0.041
0.308AsnMet: 0.308 ± 0.018
0.54AsnAsn: 0.54 ± 0.027
1.549AsnPro: 1.549 ± 0.036
0.588AsnGln: 0.588 ± 0.026
1.211AsnArg: 1.211 ± 0.03
1.1AsnSer: 1.1 ± 0.036
1.369AsnThr: 1.369 ± 0.049
1.876AsnVal: 1.876 ± 0.051
0.351AsnTrp: 0.351 ± 0.018
0.486AsnTyr: 0.486 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
6.689ProAla: 6.689 ± 0.084
0.202ProCys: 0.202 ± 0.015
3.744ProAsp: 3.744 ± 0.068
3.27ProGlu: 3.27 ± 0.055
1.698ProPhe: 1.698 ± 0.038
4.774ProGly: 4.774 ± 0.073
1.145ProHis: 1.145 ± 0.036
2.121ProIle: 2.121 ± 0.039
1.134ProLys: 1.134 ± 0.032
4.668ProLeu: 4.668 ± 0.072
0.85ProMet: 0.85 ± 0.029
1.202ProAsn: 1.202 ± 0.034
2.207ProPro: 2.207 ± 0.049
1.631ProGln: 1.631 ± 0.04
3.277ProArg: 3.277 ± 0.057
3.478ProSer: 3.478 ± 0.057
3.506ProThr: 3.506 ± 0.057
4.757ProVal: 4.757 ± 0.067
0.858ProTrp: 0.858 ± 0.032
1.121ProTyr: 1.121 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.607GlnAla: 3.607 ± 0.062
0.123GlnCys: 0.123 ± 0.009
1.161GlnAsp: 1.161 ± 0.028
1.122GlnGlu: 1.122 ± 0.031
0.85GlnPhe: 0.85 ± 0.027
2.136GlnGly: 2.136 ± 0.05
0.74GlnHis: 0.74 ± 0.022
1.302GlnIle: 1.302 ± 0.031
0.768GlnLys: 0.768 ± 0.026
3.396GlnLeu: 3.396 ± 0.061
0.454GlnMet: 0.454 ± 0.02
0.811GlnAsn: 0.811 ± 0.035
1.57GlnPro: 1.57 ± 0.042
1.417GlnGln: 1.417 ± 0.046
2.361GlnArg: 2.361 ± 0.045
1.71GlnSer: 1.71 ± 0.042
1.689GlnThr: 1.689 ± 0.042
2.453GlnVal: 2.453 ± 0.053
0.501GlnTrp: 0.501 ± 0.02
0.702GlnTyr: 0.702 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
8.708ArgAla: 8.708 ± 0.119
0.334ArgCys: 0.334 ± 0.016
4.236ArgAsp: 4.236 ± 0.07
4.035ArgGlu: 4.035 ± 0.066
2.375ArgPhe: 2.375 ± 0.048
5.25ArgGly: 5.25 ± 0.074
1.659ArgHis: 1.659 ± 0.044
3.279ArgIle: 3.279 ± 0.048
1.347ArgLys: 1.347 ± 0.034
7.263ArgLeu: 7.263 ± 0.111
1.788ArgMet: 1.788 ± 0.038
1.343ArgAsn: 1.343 ± 0.033
3.362ArgPro: 3.362 ± 0.065
2.027ArgGln: 2.027 ± 0.047
6.648ArgArg: 6.648 ± 0.112
4.298ArgSer: 4.298 ± 0.068
4.066ArgThr: 4.066 ± 0.063
5.843ArgVal: 5.843 ± 0.089
1.172ArgTrp: 1.172 ± 0.035
1.608ArgTyr: 1.608 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
7.997SerAla: 7.997 ± 0.118
0.292SerCys: 0.292 ± 0.017
3.577SerAsp: 3.577 ± 0.059
2.688SerGlu: 2.688 ± 0.052
1.982SerPhe: 1.982 ± 0.043
6.424SerGly: 6.424 ± 0.093
1.085SerHis: 1.085 ± 0.031
2.739SerIle: 2.739 ± 0.051
1.289SerLys: 1.289 ± 0.043
5.621SerLeu: 5.621 ± 0.082
1.212SerMet: 1.212 ± 0.033
1.363SerAsn: 1.363 ± 0.044
3.112SerPro: 3.112 ± 0.055
1.597SerGln: 1.597 ± 0.042
3.897SerArg: 3.897 ± 0.066
4.048SerSer: 4.048 ± 0.101
4.163SerThr: 4.163 ± 0.077
5.363SerVal: 5.363 ± 0.078
1.019SerTrp: 1.019 ± 0.029
1.294SerTyr: 1.294 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
8.298ThrAla: 8.298 ± 0.117
0.368ThrCys: 0.368 ± 0.025
3.678ThrAsp: 3.678 ± 0.058
2.722ThrGlu: 2.722 ± 0.05
1.903ThrPhe: 1.903 ± 0.039
6.042ThrGly: 6.042 ± 0.086
1.216ThrHis: 1.216 ± 0.034
3.045ThrIle: 3.045 ± 0.063
1.409ThrLys: 1.409 ± 0.035
5.966ThrLeu: 5.966 ± 0.075
1.074ThrMet: 1.074 ± 0.029
1.351ThrAsn: 1.351 ± 0.045
4.041ThrPro: 4.041 ± 0.068
1.685ThrGln: 1.685 ± 0.043
3.709ThrArg: 3.709 ± 0.056
3.874ThrSer: 3.874 ± 0.081
4.298ThrThr: 4.298 ± 0.104
6.354ThrVal: 6.354 ± 0.104
0.98ThrTrp: 0.98 ± 0.032
1.293ThrTyr: 1.293 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
11.827ValAla: 11.827 ± 0.125
0.559ValCys: 0.559 ± 0.022
5.783ValAsp: 5.783 ± 0.074
4.413ValGlu: 4.413 ± 0.069
3.073ValPhe: 3.073 ± 0.056
7.26ValGly: 7.26 ± 0.092
1.763ValHis: 1.763 ± 0.037
4.374ValIle: 4.374 ± 0.076
1.728ValLys: 1.728 ± 0.043
9.55ValLeu: 9.55 ± 0.119
1.476ValMet: 1.476 ± 0.038
1.891ValAsn: 1.891 ± 0.045
4.829ValPro: 4.829 ± 0.058
2.407ValGln: 2.407 ± 0.045
6.008ValArg: 6.008 ± 0.097
5.329ValSer: 5.329 ± 0.08
6.328ValThr: 6.328 ± 0.113
8.989ValVal: 8.989 ± 0.114
1.188ValTrp: 1.188 ± 0.035
1.564ValTyr: 1.564 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.628TrpAla: 1.628 ± 0.046
0.108TrpCys: 0.108 ± 0.01
0.766TrpAsp: 0.766 ± 0.027
0.578TrpGlu: 0.578 ± 0.021
0.568TrpPhe: 0.568 ± 0.026
1.075TrpGly: 1.075 ± 0.036
0.372TrpHis: 0.372 ± 0.019
0.738TrpIle: 0.738 ± 0.026
0.309TrpLys: 0.309 ± 0.016
1.87TrpLeu: 1.87 ± 0.053
0.406TrpMet: 0.406 ± 0.02
0.528TrpAsn: 0.528 ± 0.024
0.777TrpPro: 0.777 ± 0.029
0.564TrpGln: 0.564 ± 0.025
1.3TrpArg: 1.3 ± 0.037
1.029TrpSer: 1.029 ± 0.031
0.927TrpThr: 0.927 ± 0.03
1.173TrpVal: 1.173 ± 0.035
0.385TrpTrp: 0.385 ± 0.019
0.337TrpTyr: 0.337 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.47TyrAla: 2.47 ± 0.045
0.138TyrCys: 0.138 ± 0.011
1.436TyrAsp: 1.436 ± 0.044
1.092TyrGlu: 1.092 ± 0.031
0.703TyrPhe: 0.703 ± 0.026
1.937TyrGly: 1.937 ± 0.039
0.338TyrHis: 0.338 ± 0.019
0.708TyrIle: 0.708 ± 0.023
0.39TyrLys: 0.39 ± 0.018
2.225TyrLeu: 2.225 ± 0.045
0.258TyrMet: 0.258 ± 0.015
0.532TyrAsn: 0.532 ± 0.027
1.1TyrPro: 1.1 ± 0.03
0.634TyrGln: 0.634 ± 0.025
1.594TyrArg: 1.594 ± 0.041
1.289TyrSer: 1.289 ± 0.036
1.341TyrThr: 1.341 ± 0.046
1.733TyrVal: 1.733 ± 0.036
0.367TyrTrp: 0.367 ± 0.017
0.547TyrTyr: 0.547 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3604 proteins (1195877 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski