Amino acid dipepetide frequency for Acidocella sp. MX-AZ02

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.158AlaAla: 21.158 ± 0.229	1.255AlaCys: 1.255 ± 0.034	6.264AlaAsp: 6.264 ± 0.077	7.623AlaGlu: 7.623 ± 0.103	4.347AlaPhe: 4.347 ± 0.075	12.599AlaGly: 12.599 ± 0.133	2.76AlaHis: 2.76 ± 0.051	6.147AlaIle: 6.147 ± 0.075	4.602AlaLys: 4.602 ± 0.082	17.132AlaLeu: 17.132 ± 0.165	3.857AlaMet: 3.857 ± 0.064	3.196AlaAsn: 3.196 ± 0.063	7.271AlaPro: 7.271 ± 0.107	6.129AlaGln: 6.129 ± 0.104	9.421AlaArg: 9.421 ± 0.133	6.567AlaSer: 6.567 ± 0.09	5.989AlaThr: 5.989 ± 0.085	8.637AlaVal: 8.637 ± 0.105	1.689AlaTrp: 1.689 ± 0.041	2.789AlaTyr: 2.789 ± 0.054	0.001AlaXaa: 0.001 ± 0.001
Cys	1.334CysAla: 1.334 ± 0.035	0.126CysCys: 0.126 ± 0.011	0.498CysAsp: 0.498 ± 0.021	0.383CysGlu: 0.383 ± 0.018	0.372CysPhe: 0.372 ± 0.02	0.986CysGly: 0.986 ± 0.034	0.233CysHis: 0.233 ± 0.015	0.401CysIle: 0.401 ± 0.018	0.179CysLys: 0.179 ± 0.013	0.994CysLeu: 0.994 ± 0.031	0.144CysMet: 0.144 ± 0.012	0.204CysAsn: 0.204 ± 0.014	0.504CysPro: 0.504 ± 0.024	0.265CysGln: 0.265 ± 0.016	0.548CysArg: 0.548 ± 0.023	0.428CysSer: 0.428 ± 0.022	0.438CysThr: 0.438 ± 0.021	0.636CysVal: 0.636 ± 0.024	0.14CysTrp: 0.14 ± 0.012	0.186CysTyr: 0.186 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.492AspAla: 6.492 ± 0.087	0.411AspCys: 0.411 ± 0.02	2.213AspAsp: 2.213 ± 0.047	3.194AspGlu: 3.194 ± 0.065	2.087AspPhe: 2.087 ± 0.05	4.424AspGly: 4.424 ± 0.069	1.057AspHis: 1.057 ± 0.034	2.654AspIle: 2.654 ± 0.05	1.53AspLys: 1.53 ± 0.038	5.146AspLeu: 5.146 ± 0.081	1.228AspMet: 1.228 ± 0.04	1.162AspAsn: 1.162 ± 0.041	2.864AspPro: 2.864 ± 0.049	1.624AspGln: 1.624 ± 0.044	2.568AspArg: 2.568 ± 0.059	1.985AspSer: 1.985 ± 0.045	2.051AspThr: 2.051 ± 0.051	3.405AspVal: 3.405 ± 0.062	0.88AspTrp: 0.88 ± 0.031	1.385AspTyr: 1.385 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	8.493GluAla: 8.493 ± 0.12	0.299GluCys: 0.299 ± 0.016	2.525GluAsp: 2.525 ± 0.062	2.834GluGlu: 2.834 ± 0.068	1.715GluPhe: 1.715 ± 0.04	3.97GluGly: 3.97 ± 0.074	1.055GluHis: 1.055 ± 0.029	3.515GluIle: 3.515 ± 0.052	1.819GluLys: 1.819 ± 0.053	5.043GluLeu: 5.043 ± 0.071	1.542GluMet: 1.542 ± 0.038	1.419GluAsn: 1.419 ± 0.041	2.24GluPro: 2.24 ± 0.056	2.009GluGln: 2.009 ± 0.047	3.838GluArg: 3.838 ± 0.075	2.11GluSer: 2.11 ± 0.046	3.295GluThr: 3.295 ± 0.062	3.271GluVal: 3.271 ± 0.064	0.535GluTrp: 0.535 ± 0.023	0.91GluTyr: 0.91 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	4.873PheAla: 4.873 ± 0.07	0.534PheCys: 0.534 ± 0.023	1.935PheAsp: 1.935 ± 0.042	1.856PheGlu: 1.856 ± 0.045	1.405PhePhe: 1.405 ± 0.042	3.758PheGly: 3.758 ± 0.065	0.739PheHis: 0.739 ± 0.028	1.879PheIle: 1.879 ± 0.042	0.93PheLys: 0.93 ± 0.032	3.618PheLeu: 3.618 ± 0.073	0.863PheMet: 0.863 ± 0.029	1.13PheAsn: 1.13 ± 0.036	1.634PhePro: 1.634 ± 0.038	1.03PheGln: 1.03 ± 0.032	1.835PheArg: 1.835 ± 0.05	2.364PheSer: 2.364 ± 0.045	2.307PheThr: 2.307 ± 0.052	2.372PheVal: 2.372 ± 0.049	0.58PheTrp: 0.58 ± 0.023	0.939PheTyr: 0.939 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	10.942GlyAla: 10.942 ± 0.114	0.878GlyCys: 0.878 ± 0.027	3.698GlyAsp: 3.698 ± 0.066	4.173GlyGlu: 4.173 ± 0.069	4.006GlyPhe: 4.006 ± 0.062	7.791GlyGly: 7.791 ± 0.125	1.93GlyHis: 1.93 ± 0.052	4.608GlyIle: 4.608 ± 0.069	3.256GlyLys: 3.256 ± 0.058	10.454GlyLeu: 10.454 ± 0.105	2.524GlyMet: 2.524 ± 0.057	2.263GlyAsn: 2.263 ± 0.068	3.769GlyPro: 3.769 ± 0.066	3.661GlyGln: 3.661 ± 0.059	5.538GlyArg: 5.538 ± 0.08	4.191GlySer: 4.191 ± 0.093	4.788GlyThr: 4.788 ± 0.113	6.286GlyVal: 6.286 ± 0.09	1.648GlyTrp: 1.648 ± 0.043	2.302GlyTyr: 2.302 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.569HisAla: 2.569 ± 0.046	0.203HisCys: 0.203 ± 0.014	1.185HisAsp: 1.185 ± 0.035	1.152HisGlu: 1.152 ± 0.03	0.97HisPhe: 0.97 ± 0.036	2.116HisGly: 2.116 ± 0.048	0.612HisHis: 0.612 ± 0.032	0.991HisIle: 0.991 ± 0.027	0.597HisLys: 0.597 ± 0.025	2.256HisLeu: 2.256 ± 0.049	0.494HisMet: 0.494 ± 0.023	0.492HisAsn: 0.492 ± 0.021	1.391HisPro: 1.391 ± 0.034	0.705HisGln: 0.705 ± 0.028	1.287HisArg: 1.287 ± 0.035	0.881HisSer: 0.881 ± 0.025	0.768HisThr: 0.768 ± 0.025	1.526HisVal: 1.526 ± 0.042	0.33HisTrp: 0.33 ± 0.019	0.589HisTyr: 0.589 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	7.418IleAla: 7.418 ± 0.088	0.589IleCys: 0.589 ± 0.023	2.72IleAsp: 2.72 ± 0.049	3.23IleGlu: 3.23 ± 0.061	1.758IlePhe: 1.758 ± 0.042	4.965IleGly: 4.965 ± 0.075	0.849IleHis: 0.849 ± 0.029	2.541IleIle: 2.541 ± 0.052	1.4IleLys: 1.4 ± 0.043	4.693IleLeu: 4.693 ± 0.074	0.948IleMet: 0.948 ± 0.032	1.436IleAsn: 1.436 ± 0.042	2.294IlePro: 2.294 ± 0.048	1.3IleGln: 1.3 ± 0.033	2.795IleArg: 2.795 ± 0.051	3.092IleSer: 3.092 ± 0.066	2.99IleThr: 2.99 ± 0.06	3.555IleVal: 3.555 ± 0.067	0.632IleTrp: 0.632 ± 0.024	1.073IleTyr: 1.073 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.16LysAla: 4.16 ± 0.08	0.146LysCys: 0.146 ± 0.011	1.445LysAsp: 1.445 ± 0.039	1.356LysGlu: 1.356 ± 0.037	0.993LysPhe: 0.993 ± 0.032	2.247LysGly: 2.247 ± 0.051	0.589LysHis: 0.589 ± 0.021	1.889LysIle: 1.889 ± 0.047	1.026LysLys: 1.026 ± 0.036	3.959LysLeu: 3.959 ± 0.066	0.744LysMet: 0.744 ± 0.03	0.822LysAsn: 0.822 ± 0.029	2.153LysPro: 2.153 ± 0.046	1.146LysGln: 1.146 ± 0.035	2.153LysArg: 2.153 ± 0.047	1.676LysSer: 1.676 ± 0.041	1.78LysThr: 1.78 ± 0.044	1.918LysVal: 1.918 ± 0.051	0.264LysTrp: 0.264 ± 0.016	0.569LysTyr: 0.569 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	17.028LeuAla: 17.028 ± 0.171	1.135LeuCys: 1.135 ± 0.038	5.541LeuAsp: 5.541 ± 0.074	5.31LeuGlu: 5.31 ± 0.089	3.794LeuPhe: 3.794 ± 0.067	9.399LeuGly: 9.399 ± 0.12	2.334LeuHis: 2.334 ± 0.049	4.997LeuIle: 4.997 ± 0.071	3.371LeuLys: 3.371 ± 0.061	11.655LeuLeu: 11.655 ± 0.152	2.173LeuMet: 2.173 ± 0.05	3.034LeuAsn: 3.034 ± 0.053	6.898LeuPro: 6.898 ± 0.102	3.469LeuGln: 3.469 ± 0.067	7.916LeuArg: 7.916 ± 0.101	6.635LeuSer: 6.635 ± 0.095	5.837LeuThr: 5.837 ± 0.075	7.539LeuVal: 7.539 ± 0.096	1.298LeuTrp: 1.298 ± 0.043	2.245LeuTyr: 2.245 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	3.329MetAla: 3.329 ± 0.06	0.139MetCys: 0.139 ± 0.012	1.086MetAsp: 1.086 ± 0.034	1.097MetGlu: 1.097 ± 0.035	0.748MetPhe: 0.748 ± 0.026	1.811MetGly: 1.811 ± 0.045	0.411MetHis: 0.411 ± 0.018	1.209MetIle: 1.209 ± 0.036	0.921MetLys: 0.921 ± 0.031	2.776MetLeu: 2.776 ± 0.052	0.6MetMet: 0.6 ± 0.024	0.755MetAsn: 0.755 ± 0.026	1.472MetPro: 1.472 ± 0.037	1.004MetGln: 1.004 ± 0.028	1.713MetArg: 1.713 ± 0.042	1.647MetSer: 1.647 ± 0.039	1.386MetThr: 1.386 ± 0.033	1.679MetVal: 1.679 ± 0.041	0.172MetTrp: 0.172 ± 0.013	0.301MetTyr: 0.301 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.529AsnAla: 3.529 ± 0.065	0.255AsnCys: 0.255 ± 0.016	1.295AsnAsp: 1.295 ± 0.036	1.198AsnGlu: 1.198 ± 0.033	1.074AsnPhe: 1.074 ± 0.031	2.635AsnGly: 2.635 ± 0.061	0.531AsnHis: 0.531 ± 0.022	1.49AsnIle: 1.49 ± 0.047	0.684AsnLys: 0.684 ± 0.028	2.829AsnLeu: 2.829 ± 0.057	0.631AsnMet: 0.631 ± 0.021	0.761AsnAsn: 0.761 ± 0.039	1.888AsnPro: 1.888 ± 0.043	0.951AsnGln: 0.951 ± 0.034	1.487AsnArg: 1.487 ± 0.04	1.226AsnSer: 1.226 ± 0.045	1.213AsnThr: 1.213 ± 0.044	1.796AsnVal: 1.796 ± 0.048	0.379AsnTrp: 0.379 ± 0.019	0.668AsnTyr: 0.668 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	7.74ProAla: 7.74 ± 0.089	0.403ProCys: 0.403 ± 0.02	3.502ProAsp: 3.502 ± 0.065	3.78ProGlu: 3.78 ± 0.068	1.909ProPhe: 1.909 ± 0.047	5.677ProGly: 5.677 ± 0.077	1.233ProHis: 1.233 ± 0.029	2.079ProIle: 2.079 ± 0.042	1.516ProLys: 1.516 ± 0.035	5.25ProLeu: 5.25 ± 0.081	1.2ProMet: 1.2 ± 0.033	1.384ProAsn: 1.384 ± 0.039	3.283ProPro: 3.283 ± 0.077	1.937ProGln: 1.937 ± 0.045	2.917ProArg: 2.917 ± 0.059	2.644ProSer: 2.644 ± 0.054	2.301ProThr: 2.301 ± 0.051	4.279ProVal: 4.279 ± 0.067	0.728ProTrp: 0.728 ± 0.029	1.267ProTyr: 1.267 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	5.971GlnAla: 5.971 ± 0.098	0.22GlnCys: 0.22 ± 0.015	1.782GlnAsp: 1.782 ± 0.041	1.718GlnGlu: 1.718 ± 0.045	1.039GlnPhe: 1.039 ± 0.03	3.183GlnGly: 3.183 ± 0.057	0.756GlnHis: 0.756 ± 0.03	2.121GlnIle: 2.121 ± 0.046	1.137GlnLys: 1.137 ± 0.034	3.405GlnLeu: 3.405 ± 0.062	0.948GlnMet: 0.948 ± 0.028	1.043GlnAsn: 1.043 ± 0.032	2.016GlnPro: 2.016 ± 0.05	1.469GlnGln: 1.469 ± 0.048	2.387GlnArg: 2.387 ± 0.049	2.121GlnSer: 2.121 ± 0.055	2.04GlnThr: 2.04 ± 0.046	2.405GlnVal: 2.405 ± 0.052	0.375GlnTrp: 0.375 ± 0.018	0.643GlnTyr: 0.643 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	8.648ArgAla: 8.648 ± 0.109	0.539ArgCys: 0.539 ± 0.025	3.276ArgAsp: 3.276 ± 0.063	3.535ArgGlu: 3.535 ± 0.071	2.605ArgPhe: 2.605 ± 0.062	4.543ArgGly: 4.543 ± 0.077	1.756ArgHis: 1.756 ± 0.044	3.498ArgIle: 3.498 ± 0.064	1.932ArgLys: 1.932 ± 0.046	8.203ArgLeu: 8.203 ± 0.112	1.526ArgMet: 1.526 ± 0.039	1.65ArgAsn: 1.65 ± 0.042	3.378ArgPro: 3.378 ± 0.059	2.653ArgGln: 2.653 ± 0.059	5.049ArgArg: 5.049 ± 0.096	2.588ArgSer: 2.588 ± 0.053	2.553ArgThr: 2.553 ± 0.052	4.083ArgVal: 4.083 ± 0.07	0.813ArgTrp: 0.813 ± 0.026	1.486ArgTyr: 1.486 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.509SerAla: 6.509 ± 0.092	0.469SerCys: 0.469 ± 0.02	2.222SerAsp: 2.222 ± 0.049	2.174SerGlu: 2.174 ± 0.045	2.381SerPhe: 2.381 ± 0.047	5.435SerGly: 5.435 ± 0.103	1.049SerHis: 1.049 ± 0.034	2.526SerIle: 2.526 ± 0.054	1.293SerLys: 1.293 ± 0.039	5.919SerLeu: 5.919 ± 0.093	1.173SerMet: 1.173 ± 0.035	1.342SerAsn: 1.342 ± 0.051	2.894SerPro: 2.894 ± 0.056	1.582SerGln: 1.582 ± 0.041	2.993SerArg: 2.993 ± 0.062	2.632SerSer: 2.632 ± 0.072	2.53SerThr: 2.53 ± 0.058	3.686SerVal: 3.686 ± 0.072	0.883SerTrp: 0.883 ± 0.028	1.308SerTyr: 1.308 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	6.404ThrAla: 6.404 ± 0.086	0.358ThrCys: 0.358 ± 0.02	2.356ThrAsp: 2.356 ± 0.053	2.217ThrGlu: 2.217 ± 0.044	1.427ThrPhe: 1.427 ± 0.038	5.35ThrGly: 5.35 ± 0.08	1.13ThrHis: 1.13 ± 0.032	2.616ThrIle: 2.616 ± 0.056	1.442ThrLys: 1.442 ± 0.041	6.342ThrLeu: 6.342 ± 0.098	1.146ThrMet: 1.146 ± 0.036	1.32ThrAsn: 1.32 ± 0.042	3.514ThrPro: 3.514 ± 0.066	1.87ThrGln: 1.87 ± 0.053	3.067ThrArg: 3.067 ± 0.062	2.588ThrSer: 2.588 ± 0.059	2.573ThrThr: 2.573 ± 0.073	3.225ThrVal: 3.225 ± 0.065	0.504ThrTrp: 0.504 ± 0.022	1.128ThrTyr: 1.128 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	8.681ValAla: 8.681 ± 0.102	0.632ValCys: 0.632 ± 0.024	3.004ValAsp: 3.004 ± 0.054	3.889ValGlu: 3.889 ± 0.067	2.485ValPhe: 2.485 ± 0.048	4.777ValGly: 4.777 ± 0.086	1.185ValHis: 1.185 ± 0.034	3.655ValIle: 3.655 ± 0.061	2.37ValLys: 2.37 ± 0.057	7.809ValLeu: 7.809 ± 0.101	1.77ValMet: 1.77 ± 0.042	2.096ValAsn: 2.096 ± 0.046	3.712ValPro: 3.712 ± 0.064	2.443ValGln: 2.443 ± 0.058	4.045ValArg: 4.045 ± 0.066	3.892ValSer: 3.892 ± 0.07	4.113ValThr: 4.113 ± 0.066	4.992ValVal: 4.992 ± 0.089	0.878ValTrp: 0.878 ± 0.032	1.291ValTyr: 1.291 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.402TrpAla: 1.402 ± 0.037	0.152TrpCys: 0.152 ± 0.011	0.595TrpAsp: 0.595 ± 0.024	0.544TrpGlu: 0.544 ± 0.021	0.579TrpPhe: 0.579 ± 0.025	0.958TrpGly: 0.958 ± 0.03	0.37TrpHis: 0.37 ± 0.019	0.521TrpIle: 0.521 ± 0.024	0.36TrpLys: 0.36 ± 0.018	1.82TrpLeu: 1.82 ± 0.045	0.327TrpMet: 0.327 ± 0.018	0.382TrpAsn: 0.382 ± 0.02	0.733TrpPro: 0.733 ± 0.032	0.584TrpGln: 0.584 ± 0.026	1.23TrpArg: 1.23 ± 0.037	0.679TrpSer: 0.679 ± 0.027	0.586TrpThr: 0.586 ± 0.023	0.933TrpVal: 0.933 ± 0.027	0.212TrpTrp: 0.212 ± 0.014	0.293TrpTyr: 0.293 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.752TyrAla: 2.752 ± 0.05	0.253TyrCys: 0.253 ± 0.016	1.218TyrAsp: 1.218 ± 0.037	1.175TyrGlu: 1.175 ± 0.032	0.883TyrPhe: 0.883 ± 0.031	2.082TyrGly: 2.082 ± 0.048	0.536TyrHis: 0.536 ± 0.023	0.94TyrIle: 0.94 ± 0.028	0.669TyrLys: 0.669 ± 0.027	2.305TyrLeu: 2.305 ± 0.055	0.417TyrMet: 0.417 ± 0.02	0.629TyrAsn: 0.629 ± 0.029	1.122TyrPro: 1.122 ± 0.039	0.949TyrGln: 0.949 ± 0.036	1.529TyrArg: 1.529 ± 0.04	1.054TyrSer: 1.054 ± 0.034	1.04TyrThr: 1.04 ± 0.035	1.499TyrVal: 1.499 ± 0.039	0.32TyrTrp: 0.32 ± 0.016	0.617TyrTyr: 0.617 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3506 proteins (1058590 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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