Simian foamy virus type 3 (strain LK3) (SFVagm) (SFV-3)
Average proteome isoelectric point is 7.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P27403|BEL2_SFV3L Protein Bel-2 OS=Simian foamy virus type 3 (strain LK3) OX=11644 GN=bel2 PE=3 SV=2
MM1 pKa = 7.71 ASWEE5 pKa = 4.21 KK6 pKa = 10.64 EE7 pKa = 3.71 KK8 pKa = 11.0 EE9 pKa = 4.02 LAHH12 pKa = 6.2 LHH14 pKa = 5.49 QPEE17 pKa = 5.23 DD18 pKa = 4.06 DD19 pKa = 4.39 PLPDD23 pKa = 4.64 LSLLLDD29 pKa = 3.73 MDD31 pKa = 3.69 QFEE34 pKa = 4.58 PTEE37 pKa = 4.73 GPDD40 pKa = 3.64 SNPGAEE46 pKa = 4.66 KK47 pKa = 9.96 IYY49 pKa = 10.63 LQLQVAPGDD58 pKa = 3.95 PSEE61 pKa = 4.07 KK62 pKa = 9.32 TYY64 pKa = 11.27 KK65 pKa = 10.31 FGYY68 pKa = 8.71 EE69 pKa = 4.03 DD70 pKa = 5.55 KK71 pKa = 10.72 EE72 pKa = 4.31 AQNPDD77 pKa = 3.03 LKK79 pKa = 10.36 MRR81 pKa = 11.84 NWVPDD86 pKa = 3.93 PEE88 pKa = 5.25 KK89 pKa = 9.98 MSKK92 pKa = 8.43 WACARR97 pKa = 11.84 LILCGLYY104 pKa = 9.7 NAKK107 pKa = 9.78 KK108 pKa = 10.38 AKK110 pKa = 9.86 EE111 pKa = 4.17 LLKK114 pKa = 9.77 MDD116 pKa = 4.57 YY117 pKa = 10.23 DD118 pKa = 3.92 IHH120 pKa = 6.37 WEE122 pKa = 3.86 QSKK125 pKa = 10.86 EE126 pKa = 3.66 DD127 pKa = 3.54 SQYY130 pKa = 11.16 FEE132 pKa = 5.01 IEE134 pKa = 4.29 YY135 pKa = 9.15 HH136 pKa = 6.97 CKK138 pKa = 8.74 MCMTVIHH145 pKa = 6.6 EE146 pKa = 4.43 PMPVSYY152 pKa = 10.56 DD153 pKa = 3.16 KK154 pKa = 10.82 KK155 pKa = 7.69 TGLWIKK161 pKa = 9.43 MGPLRR166 pKa = 11.84 GDD168 pKa = 2.98 IGSVVHH174 pKa = 5.51 TCRR177 pKa = 11.84 RR178 pKa = 11.84 HH179 pKa = 4.64 YY180 pKa = 9.08 EE181 pKa = 3.92 RR182 pKa = 11.84 CMSALPSSGEE192 pKa = 3.7 PLKK195 pKa = 10.87 PRR197 pKa = 11.84 VRR199 pKa = 11.84 ANPVRR204 pKa = 11.84 RR205 pKa = 11.84 YY206 pKa = 9.68 RR207 pKa = 11.84 EE208 pKa = 3.49 KK209 pKa = 11.04 SLIVADD215 pKa = 4.19 RR216 pKa = 11.84 PKK218 pKa = 10.16 RR219 pKa = 11.84 SRR221 pKa = 11.84 WGVAPRR227 pKa = 11.84 EE228 pKa = 4.08 QPNTSSGDD236 pKa = 3.34 AMALMPGPCGPFNMDD251 pKa = 3.55 PPGCLLEE258 pKa = 4.29 RR259 pKa = 11.84 VPGSEE264 pKa = 4.13 PGTSEE269 pKa = 3.62 MALAMSGGPFWEE281 pKa = 3.89 QVYY284 pKa = 10.04 RR285 pKa = 11.84 DD286 pKa = 4.56 SISGPPTGPSEE297 pKa = 4.06 NN298 pKa = 3.71
Molecular weight: 33.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.396
IPC2_protein 5.461
IPC_protein 5.436
Toseland 5.588
ProMoST 5.664
Dawson 5.537
Bjellqvist 5.614
Wikipedia 5.474
Rodwell 5.499
Grimsley 5.601
Solomon 5.537
Lehninger 5.512
Nozaki 5.728
DTASelect 5.893
Thurlkill 5.741
EMBOSS 5.69
Sillero 5.817
Patrickios 4.088
IPC_peptide 5.563
IPC2_peptide 5.817
IPC2.peptide.svr19 5.873
Protein with the highest isoelectric point:
>sp|P27401|POL_SFV3L Pro-Pol polyprotein OS=Simian foamy virus type 3 (strain LK3) OX=11644 GN=pol PE=3 SV=2
MM1 pKa = 7.91 GDD3 pKa = 3.22 HH4 pKa = 6.6 NLNVQEE10 pKa = 5.05 LLNLFQNLGIPRR22 pKa = 11.84 QPNHH26 pKa = 6.81 RR27 pKa = 11.84 EE28 pKa = 4.07 VIGLRR33 pKa = 11.84 MLGGWWGPGTRR44 pKa = 11.84 YY45 pKa = 10.03 ILVSIFLQDD54 pKa = 4.79 DD55 pKa = 3.54 SGQPLQQPRR64 pKa = 11.84 WRR66 pKa = 11.84 PEE68 pKa = 3.58 GRR70 pKa = 11.84 PVNPLVHH77 pKa = 5.94 NTIEE81 pKa = 4.36 APWGEE86 pKa = 3.9 LRR88 pKa = 11.84 QAFEE92 pKa = 4.85 DD93 pKa = 3.92 LDD95 pKa = 3.94 VAEE98 pKa = 4.6 GTLRR102 pKa = 11.84 FGPLANGNWIPGDD115 pKa = 3.8 EE116 pKa = 4.31 YY117 pKa = 11.66 SMEE120 pKa = 4.14 FQPPLAQEE128 pKa = 4.24 IAQMQRR134 pKa = 11.84 DD135 pKa = 4.24 EE136 pKa = 4.88 LEE138 pKa = 4.5 EE139 pKa = 4.36 ILDD142 pKa = 3.6 ITGQICAQVIDD153 pKa = 4.62 LVDD156 pKa = 3.47 MQDD159 pKa = 3.02 AQIRR163 pKa = 11.84 GLEE166 pKa = 3.83 RR167 pKa = 11.84 RR168 pKa = 11.84 IQDD171 pKa = 3.49 RR172 pKa = 11.84 LGLRR176 pKa = 11.84 DD177 pKa = 4.04 NLPVAGIQAPPSSPIGQPIASSSLQPIPGSSSSPADD213 pKa = 4.0 LDD215 pKa = 5.34 GIWTPRR221 pKa = 11.84 QIDD224 pKa = 3.5 PRR226 pKa = 11.84 LSRR229 pKa = 11.84 VAYY232 pKa = 10.53 NPFLPGSSDD241 pKa = 3.2 GSGGSIPVQPSAPPAVLPSLPSLPAPVSQPIIQYY275 pKa = 8.61 VAQPPVPAPQAIPIQHH291 pKa = 5.9 IRR293 pKa = 11.84 AVTGNTPTNPRR304 pKa = 11.84 DD305 pKa = 3.23 IPMWLGRR312 pKa = 11.84 HH313 pKa = 4.88 SAAIEE318 pKa = 4.42 GVFPMTTPDD327 pKa = 3.19 LRR329 pKa = 11.84 CRR331 pKa = 11.84 VVNALIGGSLGLSLEE346 pKa = 5.09 PIHH349 pKa = 6.19 CVNWAAVVAALYY361 pKa = 10.62 VRR363 pKa = 11.84 THH365 pKa = 5.82 GSYY368 pKa = 10.49 PIHH371 pKa = 6.65 EE372 pKa = 4.66 LANVLRR378 pKa = 11.84 AVVTQEE384 pKa = 3.63 GVATGFQLGIMLSNQDD400 pKa = 3.81 YY401 pKa = 10.0 NLVWGILRR409 pKa = 11.84 PLLPGQAVVTAMQQRR424 pKa = 11.84 LDD426 pKa = 3.73 QEE428 pKa = 4.49 VNDD431 pKa = 4.23 AARR434 pKa = 11.84 ITSFNGHH441 pKa = 6.94 LNDD444 pKa = 3.95 IYY446 pKa = 11.4 QLLGLNARR454 pKa = 11.84 GQSIARR460 pKa = 11.84 AQSASTSGNSASAGRR475 pKa = 11.84 GRR477 pKa = 11.84 RR478 pKa = 11.84 GQRR481 pKa = 11.84 TQQQAGRR488 pKa = 11.84 QQQQQTRR495 pKa = 11.84 RR496 pKa = 11.84 TNQGNQGQRR505 pKa = 11.84 DD506 pKa = 3.67 NNQRR510 pKa = 11.84 QSSGGNQGQRR520 pKa = 11.84 GQGGYY525 pKa = 9.34 DD526 pKa = 3.32 LRR528 pKa = 11.84 PRR530 pKa = 11.84 TYY532 pKa = 9.72 QPQRR536 pKa = 11.84 YY537 pKa = 8.66 GGGRR541 pKa = 11.84 GRR543 pKa = 11.84 RR544 pKa = 11.84 WNDD547 pKa = 2.87 NQQQQQAQPGRR558 pKa = 11.84 SSDD561 pKa = 3.6 QPRR564 pKa = 11.84 SQSQQPQPEE573 pKa = 3.9 ARR575 pKa = 11.84 GDD577 pKa = 3.46 QSRR580 pKa = 11.84 TSGAGRR586 pKa = 11.84 GQQGRR591 pKa = 11.84 GNQNRR596 pKa = 11.84 NQRR599 pKa = 11.84 RR600 pKa = 11.84 ADD602 pKa = 3.49 ANNTRR607 pKa = 11.84 NVDD610 pKa = 3.53 TVTATTTSSSTASSGQNGSSTTPPASGSRR639 pKa = 11.84 NQGDD643 pKa = 3.19
Molecular weight: 69.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.894
IPC2_protein 8.843
IPC_protein 9.984
Toseland 9.033
ProMoST 9.253
Dawson 9.472
Bjellqvist 9.487
Wikipedia 9.794
Rodwell 9.385
Grimsley 9.604
Solomon 9.589
Lehninger 9.516
Nozaki 9.311
DTASelect 9.414
Thurlkill 9.297
EMBOSS 9.531
Sillero 9.487
Patrickios 4.94
IPC_peptide 9.56
IPC2_peptide 8.946
IPC2.peptide.svr19 8.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3454
298
1143
690.8
78.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.906 ± 0.469
1.477 ± 0.458
4.372 ± 0.339
5.472 ± 0.64
2.606 ± 0.423
5.906 ± 1.318
2.403 ± 0.249
6.514 ± 0.611
5.646 ± 1.468
9.641 ± 0.527
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.824 ± 0.417
5.067 ± 0.335
7.267 ± 0.81
6.34 ± 1.227
5.269 ± 1.04
6.601 ± 0.386
5.646 ± 0.559
6.022 ± 0.414
2.142 ± 0.241
3.88 ± 0.581
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here