Bradyrhizobium phage BDU-MI-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 207 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9SH41|A0A1X9SH41_9CAUD Uncharacterized protein OS=Bradyrhizobium phage BDU-MI-1 OX=1983459 PE=4 SV=1
MM1 pKa = 7.48SDD3 pKa = 3.53EE4 pKa = 3.99YY5 pKa = 10.9RR6 pKa = 11.84DD7 pKa = 3.59PGFVIDD13 pKa = 6.2LVTQTCVFDD22 pKa = 3.54TGFVCPIVTMLDD34 pKa = 3.26SRR36 pKa = 11.84GDD38 pKa = 3.38EE39 pKa = 3.97TLEE42 pKa = 4.11NEE44 pKa = 4.07EE45 pKa = 4.0AEE47 pKa = 4.52YY48 pKa = 11.06AVVRR52 pKa = 11.84VPPDD56 pKa = 3.5GLCITVDD63 pKa = 3.82LQALQEE69 pKa = 4.11EE70 pKa = 5.08GQLQIAHH77 pKa = 7.02

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9SHF1|A0A1X9SHF1_9CAUD Uncharacterized protein OS=Bradyrhizobium phage BDU-MI-1 OX=1983459 PE=4 SV=1
MM1 pKa = 7.27LTLNKK6 pKa = 10.11LDD8 pKa = 3.52TAEE11 pKa = 4.22AHH13 pKa = 6.8ILHH16 pKa = 6.14QKK18 pKa = 9.82LSRR21 pKa = 11.84MWRR24 pKa = 11.84EE25 pKa = 3.05ADD27 pKa = 2.94EE28 pKa = 4.56AMARR32 pKa = 11.84RR33 pKa = 11.84AYY35 pKa = 11.24RR36 pKa = 11.84MMSRR40 pKa = 11.84IYY42 pKa = 10.53LHH44 pKa = 5.61IHH46 pKa = 4.6KK47 pKa = 9.45TMLRR51 pKa = 11.84SAA53 pKa = 4.21

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

207

0

207

35136

38

1212

169.7

18.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.2 ± 0.249

1.201 ± 0.098

6.802 ± 0.168

6.233 ± 0.189

3.549 ± 0.112

7.645 ± 0.314

2.089 ± 0.108

5.399 ± 0.135

5.681 ± 0.207

7.616 ± 0.186

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.809 ± 0.094

3.671 ± 0.147

4.79 ± 0.169

3.484 ± 0.12

6.338 ± 0.193

5.39 ± 0.186

5.612 ± 0.193

6.865 ± 0.155

1.827 ± 0.07

2.798 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski