Thraustotheca clavata
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13171 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W0A7G6|A0A1W0A7G6_9STRA Alpha-carbonic anhydrase domain-containing protein (Fragment) OS=Thraustotheca clavata OX=74557 GN=THRCLA_20415 PE=4 SV=1
MM1 pKa = 8.12 DD2 pKa = 4.58 EE3 pKa = 5.39 NGNQDD8 pKa = 3.93 LLIEE12 pKa = 4.26 EE13 pKa = 4.99 EE14 pKa = 4.29 EE15 pKa = 4.56 MISADD20 pKa = 3.57 LGGGSHH26 pKa = 6.68 EE27 pKa = 4.29 EE28 pKa = 4.08 NKK30 pKa = 10.33 LDD32 pKa = 3.58 EE33 pKa = 4.64 AMGVIQDD40 pKa = 3.78 VLIDD44 pKa = 3.76 PEE46 pKa = 4.7 FVEE49 pKa = 4.77 LQNSFCEE56 pKa = 4.05 QHH58 pKa = 6.66 CEE60 pKa = 4.06 YY61 pKa = 10.89 FDD63 pKa = 5.26 DD64 pKa = 4.28 EE65 pKa = 4.99 SEE67 pKa = 4.17 NKK69 pKa = 9.82 LIYY72 pKa = 9.45 MDD74 pKa = 4.75 LFKK77 pKa = 10.82 EE78 pKa = 4.1 YY79 pKa = 10.79 TNLIEE84 pKa = 5.26 NYY86 pKa = 10.42 LEE88 pKa = 4.1 TRR90 pKa = 11.84 LKK92 pKa = 10.86 SAIDD96 pKa = 3.95 DD97 pKa = 3.95 FSMDD101 pKa = 4.41 EE102 pKa = 4.41 LCSMIQEE109 pKa = 5.09 HH110 pKa = 6.61 EE111 pKa = 4.37 DD112 pKa = 3.58 EE113 pKa = 4.42 MTGDD117 pKa = 3.91 VIDD120 pKa = 4.48 VLMSCADD127 pKa = 3.32 FDD129 pKa = 4.18 EE130 pKa = 5.66 FKK132 pKa = 11.41 SLMLSFKK139 pKa = 10.5 SQQTMLFQIQGDD151 pKa = 4.18 SLVCGG156 pKa = 3.91
Molecular weight: 17.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.452
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.541
Rodwell 3.49
Grimsley 3.363
Solomon 3.617
Lehninger 3.579
Nozaki 3.757
DTASelect 3.935
Thurlkill 3.503
EMBOSS 3.554
Sillero 3.77
Patrickios 1.024
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.719
Protein with the highest isoelectric point:
>tr|A0A1V9ZYT9|A0A1V9ZYT9_9STRA GTP-binding nuclear protein OS=Thraustotheca clavata OX=74557 GN=THRCLA_04724 PE=3 SV=1
MM1 pKa = 7.37 NFFIYY6 pKa = 10.55 SLGVDD11 pKa = 3.54 TTNQSSKK18 pKa = 11.01 VNDD21 pKa = 4.01 SIQFSFPKK29 pKa = 10.05 CQRR32 pKa = 11.84 FQVKK36 pKa = 10.32 RR37 pKa = 11.84 ITEE40 pKa = 4.42 RR41 pKa = 11.84 KK42 pKa = 9.14 QSSNQINPGIYY53 pKa = 9.52 VVRR56 pKa = 11.84 NTHH59 pKa = 5.45 TGHH62 pKa = 6.35 TFFGSTWDD70 pKa = 3.75 LEE72 pKa = 4.38 NAKK75 pKa = 9.96 KK76 pKa = 10.59 QNFQDD81 pKa = 3.77 LQLGRR86 pKa = 11.84 HH87 pKa = 5.92 EE88 pKa = 4.02 NRR90 pKa = 11.84 AMVRR94 pKa = 11.84 AVQVYY99 pKa = 8.83 GFKK102 pKa = 8.36 TTTFQILQTIPTPKK116 pKa = 9.47 EE117 pKa = 3.28 FHH119 pKa = 6.21 FRR121 pKa = 11.84 EE122 pKa = 4.37 LEE124 pKa = 3.92 NTLTQRR130 pKa = 11.84 LAFFIAKK137 pKa = 8.77 AHH139 pKa = 6.08 KK140 pKa = 8.88 VHH142 pKa = 6.51 ARR144 pKa = 11.84 KK145 pKa = 9.7 LFNQWQLQYY154 pKa = 10.32 FTFAWPMWKK163 pKa = 9.95 AHH165 pKa = 4.87 VVQTKK170 pKa = 9.56 QIEE173 pKa = 4.35 YY174 pKa = 9.14 EE175 pKa = 3.86 AATIEE180 pKa = 4.17 IQRR183 pKa = 11.84 VWRR186 pKa = 11.84 GLKK189 pKa = 10.02 GRR191 pKa = 11.84 LRR193 pKa = 11.84 AIDD196 pKa = 3.6 QRR198 pKa = 11.84 HH199 pKa = 5.42 FCCATLIQWMVRR211 pKa = 11.84 GFLAHH216 pKa = 7.04 RR217 pKa = 11.84 CLLRR221 pKa = 11.84 LRR223 pKa = 11.84 LNRR226 pKa = 11.84 KK227 pKa = 8.87 ARR229 pKa = 11.84 TIQHH233 pKa = 4.31 VAKK236 pKa = 10.51 RR237 pKa = 11.84 FLQLALYY244 pKa = 9.35 KK245 pKa = 9.9 RR246 pKa = 11.84 WKK248 pKa = 10.52 AGALLIQRR256 pKa = 11.84 VWRR259 pKa = 11.84 GVRR262 pKa = 11.84 DD263 pKa = 3.39 RR264 pKa = 11.84 RR265 pKa = 11.84 YY266 pKa = 8.72 VNQRR270 pKa = 11.84 RR271 pKa = 11.84 ILKK274 pKa = 8.61 QQNQAAFCLQRR285 pKa = 11.84 NLRR288 pKa = 11.84 LHH290 pKa = 6.45 QIHH293 pKa = 5.94 QKK295 pKa = 7.56 WLRR298 pKa = 11.84 QRR300 pKa = 11.84 RR301 pKa = 11.84 HH302 pKa = 4.71 QLHH305 pKa = 5.87 TKK307 pKa = 10.14 ASIVIQSNWRR317 pKa = 11.84 GFHH320 pKa = 5.75 GRR322 pKa = 11.84 QIALAKK328 pKa = 10.59 NILRR332 pKa = 11.84 KK333 pKa = 7.55 QQRR336 pKa = 11.84 AKK338 pKa = 10.5 NAAARR343 pKa = 11.84 LIQEE347 pKa = 4.69 SYY349 pKa = 11.13 HH350 pKa = 6.12 EE351 pKa = 4.11 YY352 pKa = 9.92 QVKK355 pKa = 10.13 KK356 pKa = 9.82 FQNAAQTIYY365 pKa = 9.6 CQKK368 pKa = 10.49 RR369 pKa = 11.84 MAVRR373 pKa = 11.84 LSVYY377 pKa = 7.33 WHH379 pKa = 6.12 NFIARR384 pKa = 11.84 KK385 pKa = 9.33 FGWAALQIHH394 pKa = 7.0 LEE396 pKa = 3.96 HH397 pKa = 7.19 KK398 pKa = 9.97 MAIKK402 pKa = 9.83 LQQWAHH408 pKa = 4.62 QRR410 pKa = 11.84 LSIKK414 pKa = 10.15 RR415 pKa = 11.84 IHH417 pKa = 6.95 RR418 pKa = 11.84 GCLQCKK424 pKa = 9.42 RR425 pKa = 11.84 IRR427 pKa = 11.84 RR428 pKa = 11.84 AICIQRR434 pKa = 11.84 NVRR437 pKa = 11.84 GFLARR442 pKa = 11.84 HH443 pKa = 6.13 IYY445 pKa = 8.57 VPRR448 pKa = 11.84 VKK450 pKa = 10.26 RR451 pKa = 11.84 EE452 pKa = 3.78 RR453 pKa = 11.84 EE454 pKa = 3.78 LKK456 pKa = 9.95 IACNYY461 pKa = 7.95 IGRR464 pKa = 11.84 WYY466 pKa = 10.68 RR467 pKa = 11.84 SLKK470 pKa = 7.36 WHH472 pKa = 6.8 RR473 pKa = 11.84 LVCFLFRR480 pKa = 11.84 TKK482 pKa = 10.55 VATQIQALFRR492 pKa = 11.84 GYY494 pKa = 9.58 VVWKK498 pKa = 10.07 HH499 pKa = 4.42 YY500 pKa = 8.54 QEE502 pKa = 5.9 CKK504 pKa = 10.4 DD505 pKa = 3.03 AWKK508 pKa = 10.37 EE509 pKa = 3.68 EE510 pKa = 4.12 KK511 pKa = 10.37 AALTIQKK518 pKa = 9.85 QYY520 pKa = 10.35 RR521 pKa = 11.84 KK522 pKa = 9.42 HH523 pKa = 6.13 IAMLKK528 pKa = 7.76 FQHH531 pKa = 6.07 RR532 pKa = 11.84 VAVLKK537 pKa = 10.94 AGACAEE543 pKa = 4.25 CGEE546 pKa = 4.88 EE547 pKa = 4.17 IATLYY552 pKa = 11.31 SMSFEE557 pKa = 4.18 MEE559 pKa = 4.13 LCHH562 pKa = 6.84 GCWSTIKK569 pKa = 10.62 GFTGTTDD576 pKa = 3.66 DD577 pKa = 3.99 KK578 pKa = 11.4 SIPIDD583 pKa = 3.31 QYY585 pKa = 10.98 RR586 pKa = 11.84 LEE588 pKa = 4.54 RR589 pKa = 11.84 LLATKK594 pKa = 10.29 LAAVYY599 pKa = 9.86 RR600 pKa = 11.84 GYY602 pKa = 11.07 AARR605 pKa = 11.84 LLYY608 pKa = 9.94 KK609 pKa = 9.96 VPCCSNCTTASRR621 pKa = 11.84 LICLEE626 pKa = 4.24 CPLILCHH633 pKa = 6.34 SCSATVHH640 pKa = 5.58 TLPYY644 pKa = 10.54 NIFHH648 pKa = 6.6 HH649 pKa = 6.25 PWRR652 pKa = 11.84 LAEE655 pKa = 4.0 HH656 pKa = 6.17 EE657 pKa = 4.43 RR658 pKa = 11.84 RR659 pKa = 11.84 LAAAIRR665 pKa = 11.84 LQTRR669 pKa = 11.84 IRR671 pKa = 11.84 CFLEE675 pKa = 4.69 RR676 pKa = 11.84 NTLQQLRR683 pKa = 11.84 DD684 pKa = 3.5 AMLLEE689 pKa = 4.72 VIIKK693 pKa = 8.81 VQGWWRR699 pKa = 11.84 TRR701 pKa = 11.84 YY702 pKa = 9.29 ARR704 pKa = 11.84 HH705 pKa = 6.38 LARR708 pKa = 11.84 VLQEE712 pKa = 3.31 QTRR715 pKa = 11.84 RR716 pKa = 11.84 YY717 pKa = 7.02 EE718 pKa = 5.17 AKK720 pKa = 9.77 RR721 pKa = 11.84 QWAAGIIQRR730 pKa = 11.84 NYY732 pKa = 8.93 RR733 pKa = 11.84 GHH735 pKa = 6.17 MGCLIAKK742 pKa = 7.65 EE743 pKa = 3.96 EE744 pKa = 3.85 RR745 pKa = 11.84 RR746 pKa = 11.84 YY747 pKa = 9.38 RR748 pKa = 11.84 QAVITIQSQFRR759 pKa = 11.84 AAIARR764 pKa = 11.84 RR765 pKa = 11.84 KK766 pKa = 8.62 VAQEE770 pKa = 3.59 RR771 pKa = 11.84 QRR773 pKa = 11.84 RR774 pKa = 11.84 LHH776 pKa = 6.23 IRR778 pKa = 11.84 RR779 pKa = 11.84 QAAALVLQCAIRR791 pKa = 11.84 QYY793 pKa = 10.63 FARR796 pKa = 11.84 QDD798 pKa = 3.19 LRR800 pKa = 11.84 RR801 pKa = 11.84 RR802 pKa = 11.84 RR803 pKa = 11.84 EE804 pKa = 3.42 FFAALRR810 pKa = 11.84 IQCTIRR816 pKa = 11.84 VYY818 pKa = 10.32 LAKK821 pKa = 10.18 QVLLALQIEE830 pKa = 4.69 YY831 pKa = 10.3 EE832 pKa = 4.36 RR833 pKa = 11.84 KK834 pKa = 8.18 FQLAIEE840 pKa = 4.1 QVKK843 pKa = 8.42 YY844 pKa = 8.42 QRR846 pKa = 11.84 KK847 pKa = 7.3 VRR849 pKa = 11.84 AVIRR853 pKa = 11.84 IQSQYY858 pKa = 9.65 RR859 pKa = 11.84 RR860 pKa = 11.84 RR861 pKa = 11.84 RR862 pKa = 11.84 DD863 pKa = 3.24 FRR865 pKa = 11.84 VAVAKK870 pKa = 10.57 RR871 pKa = 11.84 LANTKK876 pKa = 9.7 ARR878 pKa = 11.84 RR879 pKa = 11.84 EE880 pKa = 3.82 HH881 pKa = 6.22 WEE883 pKa = 4.12 RR884 pKa = 11.84 VCLLMEE890 pKa = 4.44 KK891 pKa = 10.32 SSAKK895 pKa = 10.24 RR896 pKa = 11.84 LQQWYY901 pKa = 9.08 RR902 pKa = 11.84 RR903 pKa = 11.84 RR904 pKa = 11.84 IEE906 pKa = 4.17 RR907 pKa = 11.84 LNALATRR914 pKa = 11.84 IQCMVRR920 pKa = 11.84 CHH922 pKa = 4.7 VARR925 pKa = 11.84 RR926 pKa = 11.84 VLGRR930 pKa = 11.84 HH931 pKa = 5.39 RR932 pKa = 11.84 ANAIEE937 pKa = 4.32 TKK939 pKa = 9.78 RR940 pKa = 11.84 VRR942 pKa = 11.84 AAVCLQCFSRR952 pKa = 11.84 RR953 pKa = 11.84 IIARR957 pKa = 11.84 RR958 pKa = 11.84 IVNGLRR964 pKa = 11.84 AEE966 pKa = 4.2 KK967 pKa = 10.21 QVVEE971 pKa = 4.28 NLPKK975 pKa = 9.35 EE976 pKa = 4.26 DD977 pKa = 3.76 AWIEE981 pKa = 4.08 CLDD984 pKa = 3.7 EE985 pKa = 4.74 SSGYY989 pKa = 8.23 TYY991 pKa = 10.86 YY992 pKa = 11.27 YY993 pKa = 10.54 NPQTQATTWTKK1004 pKa = 10.76 PNNTVNEE1011 pKa = 4.27 VVEE1014 pKa = 4.43 KK1015 pKa = 10.98 VDD1017 pKa = 5.82 AEE1019 pKa = 4.19 PQDD1022 pKa = 4.27 TEE1024 pKa = 5.8 WIACWDD1030 pKa = 3.96 DD1031 pKa = 3.96 SYY1033 pKa = 11.47 QAYY1036 pKa = 10.47 YY1037 pKa = 10.9 FMNKK1041 pKa = 7.24 YY1042 pKa = 9.09 TGDD1045 pKa = 4.08 TKK1047 pKa = 10.87 WEE1049 pKa = 4.43 DD1050 pKa = 3.54 PTQASYY1056 pKa = 9.78 EE1057 pKa = 4.02 WYY1059 pKa = 9.9 YY1060 pKa = 11.33 DD1061 pKa = 3.4 EE1062 pKa = 5.35 NGQVVYY1068 pKa = 10.02 YY1069 pKa = 7.52 QQPEE1073 pKa = 4.02 NQEE1076 pKa = 3.78
Molecular weight: 128.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.78
IPC_protein 10.584
Toseland 10.628
ProMoST 10.35
Dawson 10.76
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.979
Grimsley 10.818
Solomon 10.847
Lehninger 10.804
Nozaki 10.643
DTASelect 10.467
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.687
Patrickios 10.628
IPC_peptide 10.847
IPC2_peptide 9.648
IPC2.peptide.svr19 8.437
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13171
0
13171
6794705
49
10056
515.9
57.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.671 ± 0.017
1.883 ± 0.012
5.188 ± 0.016
5.917 ± 0.027
4.09 ± 0.017
5.271 ± 0.024
2.622 ± 0.01
5.902 ± 0.015
5.56 ± 0.022
9.93 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.624 ± 0.009
4.49 ± 0.012
4.718 ± 0.022
4.371 ± 0.014
4.871 ± 0.017
7.652 ± 0.025
6.142 ± 0.02
6.579 ± 0.014
1.309 ± 0.008
3.21 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here