Gordonia phage Morrissey

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gustavvirus; unclassified Gustavvirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2Z4Q7M6|A0A2Z4Q7M6_9CAUD Uncharacterized protein OS=Gordonia phage Morrissey OX=2201429 GN=56 PE=4 SV=1
MM1 pKa = 7.62SNTIVTIHH9 pKa = 5.65YY10 pKa = 8.05QSPAEE15 pKa = 4.8DD16 pKa = 3.9PLKK19 pKa = 10.73VPRR22 pKa = 11.84PTRR25 pKa = 11.84IQFDD29 pKa = 3.49DD30 pKa = 3.78AGNVKK35 pKa = 7.81TTGGEE40 pKa = 4.31GEE42 pKa = 3.98ISGDD46 pKa = 3.49QLIGLSRR53 pKa = 11.84QDD55 pKa = 3.8GPPEE59 pKa = 4.26SFDD62 pKa = 3.37AEE64 pKa = 3.99NWVEE68 pKa = 4.05AEE70 pKa = 4.09EE71 pKa = 4.91LISEE75 pKa = 4.61GVADD79 pKa = 5.28LEE81 pKa = 4.34LAGWFPVFMCGEE93 pKa = 5.03GIYY96 pKa = 8.64TWSGAVDD103 pKa = 3.4RR104 pKa = 11.84VEE106 pKa = 4.29VTVQCC111 pKa = 4.77

Molecular weight:
12.07 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2Z4Q628|A0A2Z4Q628_9CAUD Uncharacterized protein OS=Gordonia phage Morrissey OX=2201429 GN=29 PE=4 SV=1
MM1 pKa = 7.58SFPYY5 pKa = 9.83MRR7 pKa = 11.84GEE9 pKa = 4.02SARR12 pKa = 11.84VRR14 pKa = 11.84RR15 pKa = 11.84LVKK18 pKa = 10.22EE19 pKa = 3.91QAQVEE24 pKa = 4.27AASKK28 pKa = 10.07ISKK31 pKa = 10.44SLDD34 pKa = 3.27VLLSALGFVTGVTVAAWITGDD55 pKa = 3.33WEE57 pKa = 4.02ITVAQMWTAVAILAVVVAVRR77 pKa = 11.84VAWVRR82 pKa = 11.84YY83 pKa = 7.0RR84 pKa = 11.84FRR86 pKa = 11.84RR87 pKa = 11.84IVRR90 pKa = 11.84KK91 pKa = 9.19VNRR94 pKa = 11.84EE95 pKa = 4.06LEE97 pKa = 4.24VDD99 pKa = 3.96HH100 pKa = 6.94PWPDD104 pKa = 3.29SKK106 pKa = 11.72

Molecular weight:
12.04 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

14382

37

1678

217.9

23.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.542 ± 0.598

0.591 ± 0.123

6.327 ± 0.327

6.084 ± 0.348

2.503 ± 0.13

8.844 ± 0.5

1.822 ± 0.177

4.408 ± 0.17

4.13 ± 0.254

7.934 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.111

3.31 ± 0.202

5.973 ± 0.334

3.31 ± 0.151

6.39 ± 0.454

5.729 ± 0.199

7.037 ± 0.241

7.454 ± 0.241

2.009 ± 0.134

2.343 ± 0.175

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski