Gordonia phage Morrissey
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q7M6|A0A2Z4Q7M6_9CAUD Uncharacterized protein OS=Gordonia phage Morrissey OX=2201429 GN=56 PE=4 SV=1
MM1 pKa = 7.62 SNTIVTIHH9 pKa = 5.65 YY10 pKa = 8.05 QSPAEE15 pKa = 4.8 DD16 pKa = 3.9 PLKK19 pKa = 10.73 VPRR22 pKa = 11.84 PTRR25 pKa = 11.84 IQFDD29 pKa = 3.49 DD30 pKa = 3.78 AGNVKK35 pKa = 7.81 TTGGEE40 pKa = 4.31 GEE42 pKa = 3.98 ISGDD46 pKa = 3.49 QLIGLSRR53 pKa = 11.84 QDD55 pKa = 3.8 GPPEE59 pKa = 4.26 SFDD62 pKa = 3.37 AEE64 pKa = 3.99 NWVEE68 pKa = 4.05 AEE70 pKa = 4.09 EE71 pKa = 4.91 LISEE75 pKa = 4.61 GVADD79 pKa = 5.28 LEE81 pKa = 4.34 LAGWFPVFMCGEE93 pKa = 5.03 GIYY96 pKa = 8.64 TWSGAVDD103 pKa = 3.4 RR104 pKa = 11.84 VEE106 pKa = 4.29 VTVQCC111 pKa = 4.77
Molecular weight: 12.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.196
IPC2_protein 3.999
IPC_protein 3.91
Toseland 3.732
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.77
Rodwell 3.745
Grimsley 3.643
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.139
Thurlkill 3.77
EMBOSS 3.783
Sillero 4.024
Patrickios 3.427
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.945
Protein with the highest isoelectric point:
>tr|A0A2Z4Q628|A0A2Z4Q628_9CAUD Uncharacterized protein OS=Gordonia phage Morrissey OX=2201429 GN=29 PE=4 SV=1
MM1 pKa = 7.58 SFPYY5 pKa = 9.83 MRR7 pKa = 11.84 GEE9 pKa = 4.02 SARR12 pKa = 11.84 VRR14 pKa = 11.84 RR15 pKa = 11.84 LVKK18 pKa = 10.22 EE19 pKa = 3.91 QAQVEE24 pKa = 4.27 AASKK28 pKa = 10.07 ISKK31 pKa = 10.44 SLDD34 pKa = 3.27 VLLSALGFVTGVTVAAWITGDD55 pKa = 3.33 WEE57 pKa = 4.02 ITVAQMWTAVAILAVVVAVRR77 pKa = 11.84 VAWVRR82 pKa = 11.84 YY83 pKa = 7.0 RR84 pKa = 11.84 FRR86 pKa = 11.84 RR87 pKa = 11.84 IVRR90 pKa = 11.84 KK91 pKa = 9.19 VNRR94 pKa = 11.84 EE95 pKa = 4.06 LEE97 pKa = 4.24 VDD99 pKa = 3.96 HH100 pKa = 6.94 PWPDD104 pKa = 3.29 SKK106 pKa = 11.72
Molecular weight: 12.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.282
IPC2_protein 9.502
IPC_protein 10.101
Toseland 10.57
ProMoST 10.292
Dawson 10.657
Bjellqvist 10.335
Wikipedia 10.847
Rodwell 10.906
Grimsley 10.701
Solomon 10.76
Lehninger 10.73
Nozaki 10.526
DTASelect 10.335
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.584
Patrickios 10.687
IPC_peptide 10.76
IPC2_peptide 8.946
IPC2.peptide.svr19 8.842
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
66
0
66
14382
37
1678
217.9
23.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.542 ± 0.598
0.591 ± 0.123
6.327 ± 0.327
6.084 ± 0.348
2.503 ± 0.13
8.844 ± 0.5
1.822 ± 0.177
4.408 ± 0.17
4.13 ± 0.254
7.934 ± 0.312
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.26 ± 0.111
3.31 ± 0.202
5.973 ± 0.334
3.31 ± 0.151
6.39 ± 0.454
5.729 ± 0.199
7.037 ± 0.241
7.454 ± 0.241
2.009 ± 0.134
2.343 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here