Nakamurella multipartita (strain ATCC 700099 / DSM 44233 / CIP 104796 / JCM 9543 / NBRC 105858 / Y-104) (Microsphaera multipartita)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Nakamurellales; Nakamurellaceae; Nakamurella; Nakamurella multipartita

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5123 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8X8T2|C8X8T2_NAKMY Uncharacterized protein OS=Nakamurella multipartita (strain ATCC 700099 / DSM 44233 / CIP 104796 / JCM 9543 / NBRC 105858 / Y-104) OX=479431 GN=Namu_4754 PE=4 SV=1
MM1 pKa = 7.45PVIEE5 pKa = 4.27VTAGNGSLLPSSTYY19 pKa = 11.07AATLTVDD26 pKa = 3.7GAVAAGALEE35 pKa = 4.34AEE37 pKa = 4.25QAAIIVAEE45 pKa = 4.22AQAAAADD52 pKa = 4.23RR53 pKa = 11.84TAGGSVPSDD62 pKa = 3.4QSTSAAPVTISANSGEE78 pKa = 4.37CAYY81 pKa = 10.82RR82 pKa = 11.84EE83 pKa = 4.22ALLPATDD90 pKa = 4.43PAWGGNDD97 pKa = 3.06ATTGTLMVRR106 pKa = 11.84TCNGPSEE113 pKa = 4.42YY114 pKa = 10.16LYY116 pKa = 11.26VPTPPAAAGAAAAPAPPPPPDD137 pKa = 3.77PAVLAQQAYY146 pKa = 10.61AEE148 pKa = 4.62LSLPAPDD155 pKa = 4.72AYY157 pKa = 10.66RR158 pKa = 11.84SPSEE162 pKa = 4.4SNSDD166 pKa = 3.72PDD168 pKa = 3.98HH169 pKa = 6.78GGLPFTIVGLRR180 pKa = 11.84TWFWVGDD187 pKa = 3.78WQPLQRR193 pKa = 11.84TVEE196 pKa = 4.06LQGVSVTLTATPVEE210 pKa = 4.31VHH212 pKa = 6.78FDD214 pKa = 3.63PGNGDD219 pKa = 3.68SVVACAGPGRR229 pKa = 11.84AWTPADD235 pKa = 3.51GNAAPTNGGCAYY247 pKa = 8.97TYY249 pKa = 10.62TRR251 pKa = 11.84VSPDD255 pKa = 3.13RR256 pKa = 11.84PLTATTSVQWQVDD269 pKa = 3.35WTSNSGADD277 pKa = 3.44GTFPDD282 pKa = 5.03GVSQVSDD289 pKa = 3.37QLLVEE294 pKa = 4.3QIQVVVRR301 pKa = 4.17

Molecular weight: 30.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8XAI0|C8XAI0_NAKMY Uncharacterized protein OS=Nakamurella multipartita (strain ATCC 700099 / DSM 44233 / CIP 104796 / JCM 9543 / NBRC 105858 / Y-104) OX=479431 GN=Namu_0935 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.81TRR25 pKa = 11.84VARR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.68AGKK33 pKa = 9.73

Molecular weight: 4.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5123

0

5123

1712644

30

5698

334.3

35.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.896 ± 0.048

0.71 ± 0.009

6.225 ± 0.027

4.656 ± 0.029

2.768 ± 0.021

9.286 ± 0.034

2.061 ± 0.018

4.019 ± 0.023

1.682 ± 0.023

10.25 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.774 ± 0.014

1.778 ± 0.016

6.249 ± 0.03

3.067 ± 0.019

7.776 ± 0.046

5.252 ± 0.028

6.278 ± 0.041

8.883 ± 0.033

1.51 ± 0.014

1.878 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski