Beihai shrimp virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFH3|A0A1L3KFH3_9VIRU RNA replicase OS=Beihai shrimp virus 6 OX=1922672 PE=3 SV=1
MM1 pKa = 7.38EE2 pKa = 4.61VGVAQRR8 pKa = 11.84TLPSAEE14 pKa = 4.04AEE16 pKa = 4.07HH17 pKa = 8.16VIMKK21 pKa = 10.32RR22 pKa = 11.84HH23 pKa = 5.87DD24 pKa = 3.55PDD26 pKa = 3.35RR27 pKa = 11.84RR28 pKa = 11.84EE29 pKa = 3.67MLINEE34 pKa = 3.93FRR36 pKa = 11.84FGHH39 pKa = 6.84GYY41 pKa = 9.67PGEE44 pKa = 4.31SQFAARR50 pKa = 11.84LFTQSCSFLLEE61 pKa = 4.13LATDD65 pKa = 4.21FTPPEE70 pKa = 3.93GWISDD75 pKa = 3.87LVFEE79 pKa = 4.74FLPHH83 pKa = 6.3CFEE86 pKa = 4.26IVKK89 pKa = 10.3QSIHH93 pKa = 5.69FVEE96 pKa = 5.0VMAPRR101 pKa = 11.84VFWITMTTVARR112 pKa = 11.84ATVEE116 pKa = 4.05TFPEE120 pKa = 4.02YY121 pKa = 10.76LRR123 pKa = 11.84TVLRR127 pKa = 11.84ISIPVAGALQVYY139 pKa = 9.4VDD141 pKa = 3.67KK142 pKa = 11.29QNRR145 pKa = 11.84ALLKK149 pKa = 9.68PGEE152 pKa = 4.07MLFWANISCLTFRR165 pKa = 11.84VLPFQFDD172 pKa = 4.01PLVTDD177 pKa = 4.13PDD179 pKa = 3.69AVVTHH184 pKa = 6.73RR185 pKa = 11.84SISDD189 pKa = 3.34IVRR192 pKa = 11.84HH193 pKa = 5.68HH194 pKa = 6.85PNVRR198 pKa = 11.84HH199 pKa = 5.56HH200 pKa = 5.79RR201 pKa = 11.84HH202 pKa = 6.06RR203 pKa = 11.84YY204 pKa = 8.69GNTIRR209 pKa = 11.84NRR211 pKa = 11.84KK212 pKa = 4.88THH214 pKa = 5.91PP215 pKa = 3.41

Molecular weight:
24.92 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFJ9|A0A1L3KFJ9_9VIRU Capsid protein OS=Beihai shrimp virus 6 OX=1922672 PE=3 SV=1
MM1 pKa = 7.5TKK3 pKa = 10.2RR4 pKa = 11.84ANRR7 pKa = 11.84RR8 pKa = 11.84LNNAIASLQALTMATRR24 pKa = 11.84TNIRR28 pKa = 11.84AARR31 pKa = 11.84APVRR35 pKa = 11.84SAPQPRR41 pKa = 11.84PGRR44 pKa = 11.84NRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84NNRR52 pKa = 11.84GSVPGGYY59 pKa = 6.4TTGSAGPLRR68 pKa = 11.84TSGTNGGVRR77 pKa = 11.84IRR79 pKa = 11.84HH80 pKa = 5.5EE81 pKa = 4.26EE82 pKa = 3.65MFLNLRR88 pKa = 11.84PRR90 pKa = 11.84VAGEE94 pKa = 4.1NIATQIFLPGQSKK107 pKa = 9.0MPVLDD112 pKa = 3.41SFARR116 pKa = 11.84SFDD119 pKa = 3.2RR120 pKa = 11.84YY121 pKa = 9.83RR122 pKa = 11.84IHH124 pKa = 7.53SLTLRR129 pKa = 11.84YY130 pKa = 8.09KK131 pKa = 9.1TASGTTKK138 pKa = 9.65TGSVILGIDD147 pKa = 3.55GAVDD151 pKa = 3.61HH152 pKa = 6.85VPTTIAQVQGLYY164 pKa = 9.91PKK166 pKa = 9.76WRR168 pKa = 11.84GPVWGEE174 pKa = 3.53GAITANIATLMPQRR188 pKa = 11.84WLTTSTDD195 pKa = 2.99VSMLKK200 pKa = 10.31SVAHH204 pKa = 6.27AAFAVVVGLTSAEE217 pKa = 4.04PDD219 pKa = 3.51VSPGEE224 pKa = 4.0IWCSYY229 pKa = 10.29DD230 pKa = 3.39VEE232 pKa = 4.55FMYY235 pKa = 8.68PTGSGNN241 pKa = 3.18

Molecular weight:
26.16 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1372

215

916

457.3

51.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.09 ± 0.522

1.676 ± 0.428

5.248 ± 0.917

5.102 ± 0.571

3.134 ± 1.276

7.289 ± 0.973

2.843 ± 0.75

4.883 ± 0.237

4.155 ± 0.936

7.07 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.353

3.863 ± 0.331

6.122 ± 0.374

2.697 ± 0.216

8.163 ± 0.789

6.997 ± 0.682

6.633 ± 0.927

8.236 ± 0.34

1.603 ± 0.059

4.009 ± 0.992

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski