Beihai shrimp virus 6
Average proteome isoelectric point is 7.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFH3|A0A1L3KFH3_9VIRU RNA replicase OS=Beihai shrimp virus 6 OX=1922672 PE=3 SV=1
MM1 pKa = 7.38 EE2 pKa = 4.61 VGVAQRR8 pKa = 11.84 TLPSAEE14 pKa = 4.04 AEE16 pKa = 4.07 HH17 pKa = 8.16 VIMKK21 pKa = 10.32 RR22 pKa = 11.84 HH23 pKa = 5.87 DD24 pKa = 3.55 PDD26 pKa = 3.35 RR27 pKa = 11.84 RR28 pKa = 11.84 EE29 pKa = 3.67 MLINEE34 pKa = 3.93 FRR36 pKa = 11.84 FGHH39 pKa = 6.84 GYY41 pKa = 9.67 PGEE44 pKa = 4.31 SQFAARR50 pKa = 11.84 LFTQSCSFLLEE61 pKa = 4.13 LATDD65 pKa = 4.21 FTPPEE70 pKa = 3.93 GWISDD75 pKa = 3.87 LVFEE79 pKa = 4.74 FLPHH83 pKa = 6.3 CFEE86 pKa = 4.26 IVKK89 pKa = 10.3 QSIHH93 pKa = 5.69 FVEE96 pKa = 5.0 VMAPRR101 pKa = 11.84 VFWITMTTVARR112 pKa = 11.84 ATVEE116 pKa = 4.05 TFPEE120 pKa = 4.02 YY121 pKa = 10.76 LRR123 pKa = 11.84 TVLRR127 pKa = 11.84 ISIPVAGALQVYY139 pKa = 9.4 VDD141 pKa = 3.67 KK142 pKa = 11.29 QNRR145 pKa = 11.84 ALLKK149 pKa = 9.68 PGEE152 pKa = 4.07 MLFWANISCLTFRR165 pKa = 11.84 VLPFQFDD172 pKa = 4.01 PLVTDD177 pKa = 4.13 PDD179 pKa = 3.69 AVVTHH184 pKa = 6.73 RR185 pKa = 11.84 SISDD189 pKa = 3.34 IVRR192 pKa = 11.84 HH193 pKa = 5.68 HH194 pKa = 6.85 PNVRR198 pKa = 11.84 HH199 pKa = 5.56 HH200 pKa = 5.79 RR201 pKa = 11.84 HH202 pKa = 6.06 RR203 pKa = 11.84 YY204 pKa = 8.69 GNTIRR209 pKa = 11.84 NRR211 pKa = 11.84 KK212 pKa = 4.88 THH214 pKa = 5.91 PP215 pKa = 3.41
Molecular weight: 24.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.991
IPC2_protein 7.088
IPC_protein 7.337
Toseland 7.278
ProMoST 7.878
Dawson 7.863
Bjellqvist 7.878
Wikipedia 7.805
Rodwell 7.849
Grimsley 7.38
Solomon 8.024
Lehninger 8.039
Nozaki 8.185
DTASelect 8.024
Thurlkill 8.053
EMBOSS 8.156
Sillero 8.287
Patrickios 4.673
IPC_peptide 8.024
IPC2_peptide 7.658
IPC2.peptide.svr19 7.428
Protein with the highest isoelectric point:
>tr|A0A1L3KFJ9|A0A1L3KFJ9_9VIRU Capsid protein OS=Beihai shrimp virus 6 OX=1922672 PE=3 SV=1
MM1 pKa = 7.5 TKK3 pKa = 10.2 RR4 pKa = 11.84 ANRR7 pKa = 11.84 RR8 pKa = 11.84 LNNAIASLQALTMATRR24 pKa = 11.84 TNIRR28 pKa = 11.84 AARR31 pKa = 11.84 APVRR35 pKa = 11.84 SAPQPRR41 pKa = 11.84 PGRR44 pKa = 11.84 NRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 NNRR52 pKa = 11.84 GSVPGGYY59 pKa = 6.4 TTGSAGPLRR68 pKa = 11.84 TSGTNGGVRR77 pKa = 11.84 IRR79 pKa = 11.84 HH80 pKa = 5.5 EE81 pKa = 4.26 EE82 pKa = 3.65 MFLNLRR88 pKa = 11.84 PRR90 pKa = 11.84 VAGEE94 pKa = 4.1 NIATQIFLPGQSKK107 pKa = 9.0 MPVLDD112 pKa = 3.41 SFARR116 pKa = 11.84 SFDD119 pKa = 3.2 RR120 pKa = 11.84 YY121 pKa = 9.83 RR122 pKa = 11.84 IHH124 pKa = 7.53 SLTLRR129 pKa = 11.84 YY130 pKa = 8.09 KK131 pKa = 9.1 TASGTTKK138 pKa = 9.65 TGSVILGIDD147 pKa = 3.55 GAVDD151 pKa = 3.61 HH152 pKa = 6.85 VPTTIAQVQGLYY164 pKa = 9.91 PKK166 pKa = 9.76 WRR168 pKa = 11.84 GPVWGEE174 pKa = 3.53 GAITANIATLMPQRR188 pKa = 11.84 WLTTSTDD195 pKa = 2.99 VSMLKK200 pKa = 10.31 SVAHH204 pKa = 6.27 AAFAVVVGLTSAEE217 pKa = 4.04 PDD219 pKa = 3.51 VSPGEE224 pKa = 4.0 IWCSYY229 pKa = 10.29 DD230 pKa = 3.39 VEE232 pKa = 4.55 FMYY235 pKa = 8.68 PTGSGNN241 pKa = 3.18
Molecular weight: 26.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.227
IPC2_protein 10.028
IPC_protein 11.213
Toseland 11.272
ProMoST 11.667
Dawson 11.301
Bjellqvist 11.228
Wikipedia 11.725
Rodwell 11.14
Grimsley 11.345
Solomon 11.711
Lehninger 11.623
Nozaki 11.257
DTASelect 11.228
Thurlkill 11.272
EMBOSS 11.74
Sillero 11.286
Patrickios 10.862
IPC_peptide 11.711
IPC2_peptide 10.672
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1372
215
916
457.3
51.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.09 ± 0.522
1.676 ± 0.428
5.248 ± 0.917
5.102 ± 0.571
3.134 ± 1.276
7.289 ± 0.973
2.843 ± 0.75
4.883 ± 0.237
4.155 ± 0.936
7.07 ± 0.227
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.187 ± 0.353
3.863 ± 0.331
6.122 ± 0.374
2.697 ± 0.216
8.163 ± 0.789
6.997 ± 0.682
6.633 ± 0.927
8.236 ± 0.34
1.603 ± 0.059
4.009 ± 0.992
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here