Paenibacillus faecis
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5445 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5D0CYE7|A0A5D0CYE7_9BACL Type II/IV secretion system ATPase subunit OS=Paenibacillus faecis OX=862114 GN=FRY98_03575 PE=3 SV=1
MM1 pKa = 7.35 EE2 pKa = 4.03 TTICPWCQTEE12 pKa = 4.32 IVWDD16 pKa = 3.91 EE17 pKa = 3.83 EE18 pKa = 4.19 LGPEE22 pKa = 4.22 EE23 pKa = 4.24 EE24 pKa = 5.69 CPYY27 pKa = 10.62 CHH29 pKa = 7.08 NEE31 pKa = 3.65 LKK33 pKa = 10.51 GYY35 pKa = 7.56 RR36 pKa = 11.84 TLNISLGSAEE46 pKa = 4.02 EE47 pKa = 4.19 LEE49 pKa = 4.62 EE50 pKa = 3.96 EE51 pKa = 4.57 VEE53 pKa = 4.09 ADD55 pKa = 3.65 EE56 pKa = 5.82 EE57 pKa = 5.01 ADD59 pKa = 3.39 TDD61 pKa = 3.44 LWEE64 pKa = 4.45 EE65 pKa = 4.44 DD66 pKa = 4.0 EE67 pKa = 4.68 EE68 pKa = 4.78 SHH70 pKa = 7.1 LNAIRR75 pKa = 11.84 RR76 pKa = 11.84 LEE78 pKa = 4.08 SFAAAGGDD86 pKa = 3.48 LLAYY90 pKa = 9.69 EE91 pKa = 4.57 SGVEE95 pKa = 4.63 KK96 pKa = 10.55 ILDD99 pKa = 4.11 GQDD102 pKa = 3.98 EE103 pKa = 4.5 IPEE106 pKa = 4.48 CPHH109 pKa = 6.03 CRR111 pKa = 11.84 EE112 pKa = 3.94 YY113 pKa = 11.28 MMFAGTQPLGADD125 pKa = 4.27 FEE127 pKa = 4.71 PAGQEE132 pKa = 4.07 AVEE135 pKa = 4.21 QPLVAGTAKK144 pKa = 10.76 LNVFVCSACFHH155 pKa = 5.99 VSKK158 pKa = 10.47 FLSEE162 pKa = 4.42 DD163 pKa = 3.26 DD164 pKa = 3.71 RR165 pKa = 11.84 NALIRR170 pKa = 11.84 TVSGQQ175 pKa = 3.0
Molecular weight: 19.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.805
IPC2_protein 4.037
IPC_protein 3.961
Toseland 3.808
ProMoST 4.062
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.757
Rodwell 3.795
Grimsley 3.719
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.113
Thurlkill 3.808
EMBOSS 3.783
Sillero 4.075
Patrickios 1.163
IPC_peptide 3.91
IPC2_peptide 4.062
IPC2.peptide.svr19 3.959
Protein with the highest isoelectric point:
>tr|A0A5D0CRB5|A0A5D0CRB5_9BACL Uncharacterized protein OS=Paenibacillus faecis OX=862114 GN=FRY98_21165 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 8.7 PTFKK6 pKa = 10.81 PNVSKK11 pKa = 10.8 RR12 pKa = 11.84 KK13 pKa = 8.95 KK14 pKa = 8.25 VHH16 pKa = 5.49 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.78 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.23 GRR39 pKa = 11.84 KK40 pKa = 8.56 VLSAA44 pKa = 4.05
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.261
Rodwell 12.705
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.427
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.035
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5445
0
5445
1785304
25
5282
327.9
36.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.48 ± 0.041
0.75 ± 0.01
5.096 ± 0.029
7.161 ± 0.036
4.15 ± 0.029
7.837 ± 0.035
2.001 ± 0.019
6.266 ± 0.032
5.311 ± 0.031
10.28 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.734 ± 0.014
3.541 ± 0.026
4.224 ± 0.025
3.584 ± 0.021
5.601 ± 0.035
5.996 ± 0.024
5.213 ± 0.025
7.01 ± 0.026
1.273 ± 0.015
3.491 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here