Amino acid dipepetide frequency for Pseudomonas phage vB_PaeP_PAO1_1-15pyo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.045AlaAla: 13.045 ± 1.29
0.91AlaCys: 0.91 ± 0.324
5.688AlaAsp: 5.688 ± 0.616
7.964AlaGlu: 7.964 ± 1.023
4.02AlaPhe: 4.02 ± 0.536
11.604AlaGly: 11.604 ± 1.105
2.199AlaHis: 2.199 ± 0.406
3.565AlaIle: 3.565 ± 0.651
5.992AlaLys: 5.992 ± 0.827
11.073AlaLeu: 11.073 ± 0.795
3.792AlaMet: 3.792 ± 0.593
3.11AlaAsn: 3.11 ± 0.585
5.082AlaPro: 5.082 ± 0.819
5.84AlaGln: 5.84 ± 0.663
5.916AlaArg: 5.916 ± 1.011
4.551AlaSer: 4.551 ± 0.56
6.143AlaThr: 6.143 ± 0.681
8.267AlaVal: 8.267 ± 0.759
1.138AlaTrp: 1.138 ± 0.272
2.958AlaTyr: 2.958 ± 0.617
0.228AlaXaa: 0.228 ± 0.14
Cys
0.986CysAla: 0.986 ± 0.292
0.152CysCys: 0.152 ± 0.091
0.379CysAsp: 0.379 ± 0.183
0.379CysGlu: 0.379 ± 0.146
0.228CysPhe: 0.228 ± 0.135
0.607CysGly: 0.607 ± 0.27
0.228CysHis: 0.228 ± 0.15
0.607CysIle: 0.607 ± 0.31
0.379CysLys: 0.379 ± 0.198
1.062CysLeu: 1.062 ± 0.244
0.379CysMet: 0.379 ± 0.169
0.152CysAsn: 0.152 ± 0.11
0.531CysPro: 0.531 ± 0.227
0.758CysGln: 0.758 ± 0.246
0.91CysArg: 0.91 ± 0.361
0.228CysSer: 0.228 ± 0.115
0.683CysThr: 0.683 ± 0.224
0.758CysVal: 0.758 ± 0.303
0.228CysTrp: 0.228 ± 0.201
0.531CysTyr: 0.531 ± 0.223
0.0CysXaa: 0.0 ± 0.0
Asp
6.068AspAla: 6.068 ± 0.568
0.531AspCys: 0.531 ± 0.224
2.882AspAsp: 2.882 ± 0.471
4.854AspGlu: 4.854 ± 0.52
1.441AspPhe: 1.441 ± 0.344
4.247AspGly: 4.247 ± 0.687
1.062AspHis: 1.062 ± 0.252
3.11AspIle: 3.11 ± 0.424
2.351AspLys: 2.351 ± 0.455
4.93AspLeu: 4.93 ± 0.556
1.365AspMet: 1.365 ± 0.282
1.896AspAsn: 1.896 ± 0.473
3.565AspPro: 3.565 ± 0.455
2.427AspGln: 2.427 ± 0.604
3.792AspArg: 3.792 ± 0.661
3.034AspSer: 3.034 ± 0.446
4.171AspThr: 4.171 ± 0.634
3.11AspVal: 3.11 ± 0.479
1.062AspTrp: 1.062 ± 0.217
1.744AspTyr: 1.744 ± 0.408
0.076AspXaa: 0.076 ± 0.072
Glu
9.405GluAla: 9.405 ± 0.945
0.152GluCys: 0.152 ± 0.105
2.655GluAsp: 2.655 ± 0.537
3.337GluGlu: 3.337 ± 0.521
2.199GluPhe: 2.199 ± 0.371
4.778GluGly: 4.778 ± 0.794
1.441GluHis: 1.441 ± 0.284
2.427GluIle: 2.427 ± 0.333
1.896GluLys: 1.896 ± 0.314
6.219GluLeu: 6.219 ± 0.713
2.124GluMet: 2.124 ± 0.435
1.669GluAsn: 1.669 ± 0.297
1.365GluPro: 1.365 ± 0.382
3.716GluGln: 3.716 ± 0.562
4.247GluArg: 4.247 ± 0.665
2.124GluSer: 2.124 ± 0.432
2.351GluThr: 2.351 ± 0.45
4.854GluVal: 4.854 ± 0.638
0.986GluTrp: 0.986 ± 0.213
1.972GluTyr: 1.972 ± 0.402
0.0GluXaa: 0.0 ± 0.0
Phe
2.199PheAla: 2.199 ± 0.437
0.379PheCys: 0.379 ± 0.158
2.503PheAsp: 2.503 ± 0.446
2.199PheGlu: 2.199 ± 0.463
0.986PhePhe: 0.986 ± 0.261
1.517PheGly: 1.517 ± 0.338
0.607PheHis: 0.607 ± 0.205
1.441PheIle: 1.441 ± 0.301
1.744PheLys: 1.744 ± 0.255
2.503PheLeu: 2.503 ± 0.448
0.91PheMet: 0.91 ± 0.211
1.365PheAsn: 1.365 ± 0.278
1.972PhePro: 1.972 ± 0.364
1.517PheGln: 1.517 ± 0.266
2.427PheArg: 2.427 ± 0.458
2.275PheSer: 2.275 ± 0.513
1.062PheThr: 1.062 ± 0.279
1.82PheVal: 1.82 ± 0.305
0.379PheTrp: 0.379 ± 0.152
0.607PheTyr: 0.607 ± 0.237
0.0PheXaa: 0.0 ± 0.0
Gly
7.736GlyAla: 7.736 ± 0.808
1.289GlyCys: 1.289 ± 0.338
4.02GlyAsp: 4.02 ± 0.417
4.096GlyGlu: 4.096 ± 0.671
2.882GlyPhe: 2.882 ± 0.463
6.143GlyGly: 6.143 ± 1.02
1.214GlyHis: 1.214 ± 0.31
4.171GlyIle: 4.171 ± 0.411
4.247GlyLys: 4.247 ± 0.523
7.281GlyLeu: 7.281 ± 0.836
2.655GlyMet: 2.655 ± 0.453
1.82GlyAsn: 1.82 ± 0.41
2.73GlyPro: 2.73 ± 0.478
3.716GlyGln: 3.716 ± 0.623
7.281GlyArg: 7.281 ± 0.798
4.551GlySer: 4.551 ± 0.405
5.461GlyThr: 5.461 ± 0.658
6.143GlyVal: 6.143 ± 0.607
1.593GlyTrp: 1.593 ± 0.393
2.427GlyTyr: 2.427 ± 0.465
0.076GlyXaa: 0.076 ± 0.082
His
1.365HisAla: 1.365 ± 0.406
0.303HisCys: 0.303 ± 0.137
1.289HisAsp: 1.289 ± 0.284
1.517HisGlu: 1.517 ± 0.36
0.531HisPhe: 0.531 ± 0.19
1.441HisGly: 1.441 ± 0.31
0.455HisHis: 0.455 ± 0.2
0.986HisIle: 0.986 ± 0.309
1.214HisLys: 1.214 ± 0.362
1.896HisLeu: 1.896 ± 0.366
0.531HisMet: 0.531 ± 0.214
0.834HisAsn: 0.834 ± 0.205
1.062HisPro: 1.062 ± 0.353
1.138HisGln: 1.138 ± 0.216
2.048HisArg: 2.048 ± 0.44
0.91HisSer: 0.91 ± 0.289
1.289HisThr: 1.289 ± 0.361
2.048HisVal: 2.048 ± 0.547
0.455HisTrp: 0.455 ± 0.168
0.683HisTyr: 0.683 ± 0.234
0.0HisXaa: 0.0 ± 0.0
Ile
5.764IleAla: 5.764 ± 0.739
0.303IleCys: 0.303 ± 0.162
3.185IleAsp: 3.185 ± 0.632
2.427IleGlu: 2.427 ± 0.344
1.062IlePhe: 1.062 ± 0.253
2.351IleGly: 2.351 ± 0.375
1.441IleHis: 1.441 ± 0.226
1.669IleIle: 1.669 ± 0.455
2.806IleLys: 2.806 ± 0.475
2.351IleLeu: 2.351 ± 0.503
1.365IleMet: 1.365 ± 0.308
1.896IleAsn: 1.896 ± 0.324
2.048IlePro: 2.048 ± 0.391
1.972IleGln: 1.972 ± 0.327
2.427IleArg: 2.427 ± 0.379
1.062IleSer: 1.062 ± 0.246
2.579IleThr: 2.579 ± 0.496
2.655IleVal: 2.655 ± 0.491
0.379IleTrp: 0.379 ± 0.164
0.91IleTyr: 0.91 ± 0.276
0.0IleXaa: 0.0 ± 0.0
Lys
6.143LysAla: 6.143 ± 0.675
0.228LysCys: 0.228 ± 0.14
3.185LysAsp: 3.185 ± 0.533
2.275LysGlu: 2.275 ± 0.522
1.517LysPhe: 1.517 ± 0.293
4.551LysGly: 4.551 ± 0.489
0.758LysHis: 0.758 ± 0.291
1.214LysIle: 1.214 ± 0.32
1.896LysLys: 1.896 ± 0.41
3.944LysLeu: 3.944 ± 0.705
1.82LysMet: 1.82 ± 0.312
1.062LysAsn: 1.062 ± 0.324
2.275LysPro: 2.275 ± 0.383
2.73LysGln: 2.73 ± 0.465
2.73LysArg: 2.73 ± 0.412
2.048LysSer: 2.048 ± 0.446
2.199LysThr: 2.199 ± 0.375
3.413LysVal: 3.413 ± 0.523
0.607LysTrp: 0.607 ± 0.211
0.91LysTyr: 0.91 ± 0.244
0.0LysXaa: 0.0 ± 0.0
Leu
10.163LeuAla: 10.163 ± 0.763
0.91LeuCys: 0.91 ± 0.282
6.295LeuAsp: 6.295 ± 0.754
5.233LeuGlu: 5.233 ± 0.573
1.744LeuPhe: 1.744 ± 0.389
7.205LeuGly: 7.205 ± 0.904
2.73LeuHis: 2.73 ± 0.452
3.034LeuIle: 3.034 ± 0.382
3.641LeuLys: 3.641 ± 0.366
6.75LeuLeu: 6.75 ± 0.665
2.427LeuMet: 2.427 ± 0.42
3.565LeuAsn: 3.565 ± 0.422
4.702LeuPro: 4.702 ± 0.687
3.489LeuGln: 3.489 ± 0.479
6.674LeuArg: 6.674 ± 0.749
4.778LeuSer: 4.778 ± 0.598
5.006LeuThr: 5.006 ± 0.687
4.93LeuVal: 4.93 ± 0.494
1.365LeuTrp: 1.365 ± 0.235
3.337LeuTyr: 3.337 ± 0.555
0.0LeuXaa: 0.0 ± 0.0
Met
4.323MetAla: 4.323 ± 0.56
0.076MetCys: 0.076 ± 0.084
2.275MetAsp: 2.275 ± 0.263
1.517MetGlu: 1.517 ± 0.42
0.531MetPhe: 0.531 ± 0.239
2.503MetGly: 2.503 ± 0.33
0.986MetHis: 0.986 ± 0.293
0.531MetIle: 0.531 ± 0.192
1.062MetLys: 1.062 ± 0.277
2.351MetLeu: 2.351 ± 0.428
0.91MetMet: 0.91 ± 0.249
0.91MetAsn: 0.91 ± 0.342
1.593MetPro: 1.593 ± 0.297
1.82MetGln: 1.82 ± 0.375
2.048MetArg: 2.048 ± 0.33
2.124MetSer: 2.124 ± 0.39
2.958MetThr: 2.958 ± 0.34
0.758MetVal: 0.758 ± 0.223
0.303MetTrp: 0.303 ± 0.146
0.683MetTyr: 0.683 ± 0.197
0.0MetXaa: 0.0 ± 0.0
Asn
3.868AsnAla: 3.868 ± 0.581
0.076AsnCys: 0.076 ± 0.079
1.593AsnAsp: 1.593 ± 0.299
1.896AsnGlu: 1.896 ± 0.321
0.758AsnPhe: 0.758 ± 0.25
2.503AsnGly: 2.503 ± 0.444
0.531AsnHis: 0.531 ± 0.201
1.138AsnIle: 1.138 ± 0.264
1.82AsnLys: 1.82 ± 0.369
3.565AsnLeu: 3.565 ± 0.689
0.607AsnMet: 0.607 ± 0.184
1.289AsnAsn: 1.289 ± 0.37
2.199AsnPro: 2.199 ± 0.428
1.517AsnGln: 1.517 ± 0.315
1.82AsnArg: 1.82 ± 0.323
1.214AsnSer: 1.214 ± 0.443
2.351AsnThr: 2.351 ± 0.47
3.489AsnVal: 3.489 ± 0.63
1.062AsnTrp: 1.062 ± 0.294
1.062AsnTyr: 1.062 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
5.916ProAla: 5.916 ± 1.106
0.379ProCys: 0.379 ± 0.185
2.427ProAsp: 2.427 ± 0.478
4.171ProGlu: 4.171 ± 0.537
1.214ProPhe: 1.214 ± 0.308
4.93ProGly: 4.93 ± 0.735
0.758ProHis: 0.758 ± 0.231
1.669ProIle: 1.669 ± 0.384
2.427ProLys: 2.427 ± 0.338
3.868ProLeu: 3.868 ± 0.634
0.91ProMet: 0.91 ± 0.267
2.275ProAsn: 2.275 ± 0.455
2.124ProPro: 2.124 ± 0.64
1.82ProGln: 1.82 ± 0.711
2.882ProArg: 2.882 ± 0.419
2.579ProSer: 2.579 ± 0.414
2.806ProThr: 2.806 ± 0.473
2.958ProVal: 2.958 ± 0.665
0.834ProTrp: 0.834 ± 0.241
1.669ProTyr: 1.669 ± 0.408
0.0ProXaa: 0.0 ± 0.0
Gln
7.509GlnAla: 7.509 ± 1.096
0.683GlnCys: 0.683 ± 0.218
1.82GlnAsp: 1.82 ± 0.274
2.882GlnGlu: 2.882 ± 0.461
1.365GlnPhe: 1.365 ± 0.348
4.171GlnGly: 4.171 ± 0.521
0.683GlnHis: 0.683 ± 0.218
1.972GlnIle: 1.972 ± 0.301
1.062GlnLys: 1.062 ± 0.257
4.02GlnLeu: 4.02 ± 0.502
1.214GlnMet: 1.214 ± 0.298
1.441GlnAsn: 1.441 ± 0.227
2.275GlnPro: 2.275 ± 0.774
2.579GlnGln: 2.579 ± 0.477
3.868GlnArg: 3.868 ± 0.628
2.199GlnSer: 2.199 ± 0.478
1.972GlnThr: 1.972 ± 0.333
3.261GlnVal: 3.261 ± 0.492
0.758GlnTrp: 0.758 ± 0.307
1.896GlnTyr: 1.896 ± 0.405
0.0GlnXaa: 0.0 ± 0.0
Arg
7.584ArgAla: 7.584 ± 1.074
0.91ArgCys: 0.91 ± 0.342
4.551ArgAsp: 4.551 ± 0.458
4.323ArgGlu: 4.323 ± 0.657
3.034ArgPhe: 3.034 ± 0.476
5.461ArgGly: 5.461 ± 0.687
1.669ArgHis: 1.669 ± 0.298
3.261ArgIle: 3.261 ± 0.474
3.261ArgLys: 3.261 ± 0.627
5.612ArgLeu: 5.612 ± 0.582
2.503ArgMet: 2.503 ± 0.417
3.337ArgAsn: 3.337 ± 0.531
2.882ArgPro: 2.882 ± 0.497
2.351ArgGln: 2.351 ± 0.377
6.75ArgArg: 6.75 ± 0.693
4.399ArgSer: 4.399 ± 0.664
3.261ArgThr: 3.261 ± 0.516
4.626ArgVal: 4.626 ± 0.687
1.517ArgTrp: 1.517 ± 0.372
2.199ArgTyr: 2.199 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
4.93SerAla: 4.93 ± 0.49
0.683SerCys: 0.683 ± 0.22
2.73SerAsp: 2.73 ± 0.443
2.882SerGlu: 2.882 ± 0.397
1.138SerPhe: 1.138 ± 0.313
4.096SerGly: 4.096 ± 0.447
0.986SerHis: 0.986 ± 0.323
2.351SerIle: 2.351 ± 0.564
2.503SerLys: 2.503 ± 0.427
4.778SerLeu: 4.778 ± 0.616
1.441SerMet: 1.441 ± 0.344
1.289SerAsn: 1.289 ± 0.246
2.579SerPro: 2.579 ± 0.384
2.579SerGln: 2.579 ± 0.461
4.778SerArg: 4.778 ± 0.727
1.214SerSer: 1.214 ± 0.323
2.427SerThr: 2.427 ± 0.614
2.958SerVal: 2.958 ± 0.482
0.379SerTrp: 0.379 ± 0.161
1.517SerTyr: 1.517 ± 0.361
0.0SerXaa: 0.0 ± 0.0
Thr
6.447ThrAla: 6.447 ± 0.916
0.834ThrCys: 0.834 ± 0.252
3.413ThrAsp: 3.413 ± 0.549
2.048ThrGlu: 2.048 ± 0.341
1.896ThrPhe: 1.896 ± 0.342
4.475ThrGly: 4.475 ± 0.58
1.517ThrHis: 1.517 ± 0.231
3.413ThrIle: 3.413 ± 0.409
2.882ThrLys: 2.882 ± 0.498
4.854ThrLeu: 4.854 ± 0.581
1.289ThrMet: 1.289 ± 0.318
1.896ThrAsn: 1.896 ± 0.412
3.489ThrPro: 3.489 ± 0.393
2.199ThrGln: 2.199 ± 0.373
3.11ThrArg: 3.11 ± 0.439
3.565ThrSer: 3.565 ± 0.546
2.806ThrThr: 2.806 ± 0.635
2.958ThrVal: 2.958 ± 0.55
0.986ThrTrp: 0.986 ± 0.247
1.896ThrTyr: 1.896 ± 0.388
0.0ThrXaa: 0.0 ± 0.0
Val
6.295ValAla: 6.295 ± 0.678
0.834ValCys: 0.834 ± 0.289
4.475ValAsp: 4.475 ± 0.569
3.641ValGlu: 3.641 ± 0.484
2.275ValPhe: 2.275 ± 0.401
5.612ValGly: 5.612 ± 0.673
1.365ValHis: 1.365 ± 0.456
2.199ValIle: 2.199 ± 0.506
2.73ValLys: 2.73 ± 0.386
6.523ValLeu: 6.523 ± 0.68
2.579ValMet: 2.579 ± 0.374
2.124ValAsn: 2.124 ± 0.31
3.641ValPro: 3.641 ± 1.007
3.185ValGln: 3.185 ± 0.515
6.068ValArg: 6.068 ± 0.658
2.351ValSer: 2.351 ± 0.409
3.641ValThr: 3.641 ± 0.619
4.854ValVal: 4.854 ± 0.707
0.303ValTrp: 0.303 ± 0.142
1.972ValTyr: 1.972 ± 0.437
0.0ValXaa: 0.0 ± 0.0
Trp
1.138TrpAla: 1.138 ± 0.289
0.303TrpCys: 0.303 ± 0.132
1.138TrpAsp: 1.138 ± 0.334
0.683TrpGlu: 0.683 ± 0.234
0.607TrpPhe: 0.607 ± 0.25
0.91TrpGly: 0.91 ± 0.296
0.455TrpHis: 0.455 ± 0.269
0.683TrpIle: 0.683 ± 0.274
0.531TrpLys: 0.531 ± 0.187
1.214TrpLeu: 1.214 ± 0.239
0.531TrpMet: 0.531 ± 0.165
0.986TrpAsn: 0.986 ± 0.223
0.607TrpPro: 0.607 ± 0.208
0.758TrpGln: 0.758 ± 0.313
1.365TrpArg: 1.365 ± 0.337
1.138TrpSer: 1.138 ± 0.291
0.683TrpThr: 0.683 ± 0.197
0.834TrpVal: 0.834 ± 0.319
0.228TrpTrp: 0.228 ± 0.174
0.607TrpTyr: 0.607 ± 0.211
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.124TyrAla: 2.124 ± 0.446
0.228TyrCys: 0.228 ± 0.162
1.214TyrAsp: 1.214 ± 0.337
1.593TyrGlu: 1.593 ± 0.291
0.91TyrPhe: 0.91 ± 0.207
2.427TyrGly: 2.427 ± 0.38
0.91TyrHis: 0.91 ± 0.253
1.214TyrIle: 1.214 ± 0.345
1.062TyrLys: 1.062 ± 0.323
3.11TyrLeu: 3.11 ± 0.411
0.834TyrMet: 0.834 ± 0.22
1.441TyrAsn: 1.441 ± 0.338
1.82TyrPro: 1.82 ± 0.4
1.517TyrGln: 1.517 ± 0.325
2.351TyrArg: 2.351 ± 0.36
1.896TyrSer: 1.896 ± 0.387
2.124TyrThr: 2.124 ± 0.465
1.972TyrVal: 1.972 ± 0.305
0.834TyrTrp: 0.834 ± 0.204
0.455TyrTyr: 0.455 ± 0.294
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.076XaaAla: 0.076 ± 0.082
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.076XaaPhe: 0.076 ± 0.076
0.076XaaGly: 0.076 ± 0.086
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.076XaaLeu: 0.076 ± 0.068
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.076XaaPro: 0.076 ± 0.072
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (13186 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski