Anguilla anguilla circovirus
Average proteome isoelectric point is 8.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5RNT1|W5RNT1_9CIRC ATP-dependent helicase Rep OS=Anguilla anguilla circovirus OX=1420600 PE=3 SV=1
MM1 pKa = 7.35 SKK3 pKa = 10.17 VSVRR7 pKa = 11.84 RR8 pKa = 11.84 VCFTLNNYY16 pKa = 7.58 TEE18 pKa = 4.44 EE19 pKa = 3.99 DD20 pKa = 3.56 LKK22 pKa = 11.29 FIEE25 pKa = 4.36 EE26 pKa = 4.27 HH27 pKa = 6.01 FKK29 pKa = 11.1 SVSKK33 pKa = 10.44 YY34 pKa = 10.42 LIVGKK39 pKa = 10.41 KK40 pKa = 9.6 VGEE43 pKa = 4.19 NGTPHH48 pKa = 5.74 SQGFINLKK56 pKa = 10.34 KK57 pKa = 8.79 KK58 pKa = 7.2 THH60 pKa = 5.84 FNSVKK65 pKa = 10.21 KK66 pKa = 10.32 LLPRR70 pKa = 11.84 AHH72 pKa = 6.6 IEE74 pKa = 3.88 KK75 pKa = 10.78 AKK77 pKa = 11.22 GNDD80 pKa = 3.4 QHH82 pKa = 7.21 NKK84 pKa = 10.14 DD85 pKa = 3.8 YY86 pKa = 11.05 CSKK89 pKa = 10.51 QEE91 pKa = 3.61 VAFEE95 pKa = 4.23 HH96 pKa = 6.65 GQIQGQGKK104 pKa = 10.3 RR105 pKa = 11.84 NDD107 pKa = 3.81 LADD110 pKa = 3.66 ALATVRR116 pKa = 11.84 ANGGDD121 pKa = 3.21 IKK123 pKa = 11.07 ALEE126 pKa = 4.7 DD127 pKa = 3.61 SHH129 pKa = 8.15 SLVYY133 pKa = 10.52 AKK135 pKa = 10.34 YY136 pKa = 10.13 KK137 pKa = 10.5 RR138 pKa = 11.84 GILACIDD145 pKa = 3.26 DD146 pKa = 4.75 FGWKK150 pKa = 9.81 KK151 pKa = 9.84 PRR153 pKa = 11.84 DD154 pKa = 3.82 WKK156 pKa = 10.1 TEE158 pKa = 3.6 VHH160 pKa = 6.0 VLWGIPGCGKK170 pKa = 10.0 SRR172 pKa = 11.84 YY173 pKa = 9.38 ARR175 pKa = 11.84 EE176 pKa = 4.07 QAPEE180 pKa = 3.93 AYY182 pKa = 9.5 YY183 pKa = 10.51 KK184 pKa = 10.55 PRR186 pKa = 11.84 GEE188 pKa = 3.71 WWDD191 pKa = 3.87 GYY193 pKa = 9.27 TGQEE197 pKa = 4.77 DD198 pKa = 4.49 VILDD202 pKa = 4.42 DD203 pKa = 4.22 FYY205 pKa = 11.95 GWLKK209 pKa = 10.53 FDD211 pKa = 4.66 EE212 pKa = 4.59 LLKK215 pKa = 10.71 ICDD218 pKa = 3.7 RR219 pKa = 11.84 YY220 pKa = 10.06 PYY222 pKa = 9.68 RR223 pKa = 11.84 VPVKK227 pKa = 10.48 GSFVQFTAKK236 pKa = 10.29 RR237 pKa = 11.84 IYY239 pKa = 9.11 ITSNVAASEE248 pKa = 4.35 WYY250 pKa = 9.97 HH251 pKa = 5.14 FQGYY255 pKa = 10.15 DD256 pKa = 3.05 PTALYY261 pKa = 10.9 RR262 pKa = 11.84 RR263 pKa = 11.84 MTSYY267 pKa = 9.43 LTWNDD272 pKa = 2.68 MCKK275 pKa = 10.07 EE276 pKa = 3.87 FRR278 pKa = 11.84 PMPININYY286 pKa = 9.57
Molecular weight: 33.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.514
IPC2_protein 8.448
IPC_protein 8.331
Toseland 8.873
ProMoST 8.887
Dawson 9.238
Bjellqvist 9.121
Wikipedia 9.399
Rodwell 9.399
Grimsley 9.253
Solomon 9.297
Lehninger 9.268
Nozaki 9.209
DTASelect 9.019
Thurlkill 9.121
EMBOSS 9.37
Sillero 9.297
Patrickios 4.393
IPC_peptide 9.297
IPC2_peptide 7.819
IPC2.peptide.svr19 7.706
Protein with the highest isoelectric point:
>tr|W5RNT1|W5RNT1_9CIRC ATP-dependent helicase Rep OS=Anguilla anguilla circovirus OX=1420600 PE=3 SV=1
MM1 pKa = 7.48 PFYY4 pKa = 10.62 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 VYY9 pKa = 10.0 RR10 pKa = 11.84 RR11 pKa = 11.84 KK12 pKa = 10.1 SNRR15 pKa = 11.84 RR16 pKa = 11.84 PIRR19 pKa = 11.84 NCQRR23 pKa = 11.84 RR24 pKa = 11.84 YY25 pKa = 9.07 RR26 pKa = 11.84 RR27 pKa = 11.84 PIRR30 pKa = 11.84 RR31 pKa = 11.84 EE32 pKa = 3.59 RR33 pKa = 11.84 NNRR36 pKa = 11.84 MAVSHH41 pKa = 6.67 HH42 pKa = 6.47 RR43 pKa = 11.84 LQIIDD48 pKa = 4.94 HH49 pKa = 6.01 IQMPNTANTNQTYY62 pKa = 10.87 SRR64 pKa = 11.84 FSRR67 pKa = 11.84 FFWKK71 pKa = 9.78 PSKK74 pKa = 10.25 VARR77 pKa = 11.84 ATPSQVPSFQAHH89 pKa = 5.86 GFKK92 pKa = 10.5 CVNRR96 pKa = 11.84 RR97 pKa = 11.84 AGPSLCSGPPGWNLRR112 pKa = 11.84 SAA114 pKa = 3.93
Molecular weight: 13.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.205
IPC2_protein 10.643
IPC_protein 11.974
Toseland 12.12
ProMoST 12.618
Dawson 12.12
Bjellqvist 12.12
Wikipedia 12.603
Rodwell 11.74
Grimsley 12.164
Solomon 12.618
Lehninger 12.515
Nozaki 12.12
DTASelect 12.12
Thurlkill 12.12
EMBOSS 12.618
Sillero 12.12
Patrickios 11.462
IPC_peptide 12.618
IPC2_peptide 11.608
IPC2.peptide.svr19 9.229
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
400
114
286
200.0
23.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.0 ± 0.073
2.25 ± 0.198
4.75 ± 2.007
4.5 ± 1.878
4.75 ± 0.266
6.25 ± 1.421
3.25 ± 0.134
5.0 ± 0.318
8.25 ± 2.457
5.25 ± 1.357
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.75 ± 0.457
5.5 ± 1.241
5.25 ± 1.826
4.0 ± 0.655
9.75 ± 5.404
6.0 ± 1.437
4.0 ± 0.255
5.5 ± 0.577
2.5 ± 0.386
5.5 ± 1.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here