Anguilla anguilla circovirus 
Average proteome isoelectric point is 8.86 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|W5RNT1|W5RNT1_9CIRC ATP-dependent helicase Rep OS=Anguilla anguilla circovirus OX=1420600 PE=3 SV=1MM1 pKa = 7.35  SKK3 pKa = 10.17  VSVRR7 pKa = 11.84  RR8 pKa = 11.84  VCFTLNNYY16 pKa = 7.58  TEE18 pKa = 4.44  EE19 pKa = 3.99  DD20 pKa = 3.56  LKK22 pKa = 11.29  FIEE25 pKa = 4.36  EE26 pKa = 4.27  HH27 pKa = 6.01  FKK29 pKa = 11.1  SVSKK33 pKa = 10.44  YY34 pKa = 10.42  LIVGKK39 pKa = 10.41  KK40 pKa = 9.6  VGEE43 pKa = 4.19  NGTPHH48 pKa = 5.74  SQGFINLKK56 pKa = 10.34  KK57 pKa = 8.79  KK58 pKa = 7.2  THH60 pKa = 5.84  FNSVKK65 pKa = 10.21  KK66 pKa = 10.32  LLPRR70 pKa = 11.84  AHH72 pKa = 6.6  IEE74 pKa = 3.88  KK75 pKa = 10.78  AKK77 pKa = 11.22  GNDD80 pKa = 3.4  QHH82 pKa = 7.21  NKK84 pKa = 10.14  DD85 pKa = 3.8  YY86 pKa = 11.05  CSKK89 pKa = 10.51  QEE91 pKa = 3.61  VAFEE95 pKa = 4.23  HH96 pKa = 6.65  GQIQGQGKK104 pKa = 10.3  RR105 pKa = 11.84  NDD107 pKa = 3.81  LADD110 pKa = 3.66  ALATVRR116 pKa = 11.84  ANGGDD121 pKa = 3.21  IKK123 pKa = 11.07  ALEE126 pKa = 4.7  DD127 pKa = 3.61  SHH129 pKa = 8.15  SLVYY133 pKa = 10.52  AKK135 pKa = 10.34  YY136 pKa = 10.13  KK137 pKa = 10.5  RR138 pKa = 11.84  GILACIDD145 pKa = 3.26  DD146 pKa = 4.75  FGWKK150 pKa = 9.81  KK151 pKa = 9.84  PRR153 pKa = 11.84  DD154 pKa = 3.82  WKK156 pKa = 10.1  TEE158 pKa = 3.6  VHH160 pKa = 6.0  VLWGIPGCGKK170 pKa = 10.0  SRR172 pKa = 11.84  YY173 pKa = 9.38  ARR175 pKa = 11.84  EE176 pKa = 4.07  QAPEE180 pKa = 3.93  AYY182 pKa = 9.5  YY183 pKa = 10.51  KK184 pKa = 10.55  PRR186 pKa = 11.84  GEE188 pKa = 3.71  WWDD191 pKa = 3.87  GYY193 pKa = 9.27  TGQEE197 pKa = 4.77  DD198 pKa = 4.49  VILDD202 pKa = 4.42  DD203 pKa = 4.22  FYY205 pKa = 11.95  GWLKK209 pKa = 10.53  FDD211 pKa = 4.66  EE212 pKa = 4.59  LLKK215 pKa = 10.71  ICDD218 pKa = 3.7  RR219 pKa = 11.84  YY220 pKa = 10.06  PYY222 pKa = 9.68  RR223 pKa = 11.84  VPVKK227 pKa = 10.48  GSFVQFTAKK236 pKa = 10.29  RR237 pKa = 11.84  IYY239 pKa = 9.11  ITSNVAASEE248 pKa = 4.35  WYY250 pKa = 9.97  HH251 pKa = 5.14  FQGYY255 pKa = 10.15  DD256 pKa = 3.05  PTALYY261 pKa = 10.9  RR262 pKa = 11.84  RR263 pKa = 11.84  MTSYY267 pKa = 9.43  LTWNDD272 pKa = 2.68  MCKK275 pKa = 10.07  EE276 pKa = 3.87  FRR278 pKa = 11.84  PMPININYY286 pKa = 9.57  
 33.31 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  8.514 
IPC2_protein 8.448 
IPC_protein 8.331 
Toseland    8.873 
ProMoST     8.887 
Dawson      9.238 
Bjellqvist  9.121 
Wikipedia   9.399 
Rodwell     9.399 
Grimsley    9.253 
Solomon     9.297 
Lehninger   9.268 
Nozaki      9.209 
DTASelect   9.019 
Thurlkill   9.121 
EMBOSS      9.37 
Sillero     9.297 
Patrickios  4.393 
IPC_peptide 9.297 
IPC2_peptide  7.819 
IPC2.peptide.svr19  7.706 
 Protein with the highest isoelectric point: 
>tr|W5RNT1|W5RNT1_9CIRC ATP-dependent helicase Rep OS=Anguilla anguilla circovirus OX=1420600 PE=3 SV=1MM1 pKa = 7.48  PFYY4 pKa = 10.62  RR5 pKa = 11.84  RR6 pKa = 11.84  RR7 pKa = 11.84  VYY9 pKa = 10.0  RR10 pKa = 11.84  RR11 pKa = 11.84  KK12 pKa = 10.1  SNRR15 pKa = 11.84  RR16 pKa = 11.84  PIRR19 pKa = 11.84  NCQRR23 pKa = 11.84  RR24 pKa = 11.84  YY25 pKa = 9.07  RR26 pKa = 11.84  RR27 pKa = 11.84  PIRR30 pKa = 11.84  RR31 pKa = 11.84  EE32 pKa = 3.59  RR33 pKa = 11.84  NNRR36 pKa = 11.84  MAVSHH41 pKa = 6.67  HH42 pKa = 6.47  RR43 pKa = 11.84  LQIIDD48 pKa = 4.94  HH49 pKa = 6.01  IQMPNTANTNQTYY62 pKa = 10.87  SRR64 pKa = 11.84  FSRR67 pKa = 11.84  FFWKK71 pKa = 9.78  PSKK74 pKa = 10.25  VARR77 pKa = 11.84  ATPSQVPSFQAHH89 pKa = 5.86  GFKK92 pKa = 10.5  CVNRR96 pKa = 11.84  RR97 pKa = 11.84  AGPSLCSGPPGWNLRR112 pKa = 11.84  SAA114 pKa = 3.93  
 13.7 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.205 
IPC2_protein 10.643 
IPC_protein 11.974 
Toseland    12.12 
ProMoST     12.618 
Dawson      12.12 
Bjellqvist  12.12 
Wikipedia   12.603 
Rodwell     11.74 
Grimsley    12.164 
Solomon     12.618 
Lehninger   12.515 
Nozaki      12.12 
DTASelect   12.12 
Thurlkill   12.12 
EMBOSS      12.618 
Sillero     12.12 
Patrickios  11.462 
IPC_peptide 12.618 
IPC2_peptide  11.608 
IPC2.peptide.svr19  9.229 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2 
0
2 
400
114
286
200.0
23.5
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        6.0 ± 0.073
2.25 ± 0.198
4.75 ± 2.007
4.5 ± 1.878
4.75 ± 0.266
6.25 ± 1.421
3.25 ± 0.134
5.0 ± 0.318
8.25 ± 2.457
5.25 ± 1.357
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.75 ± 0.457
5.5 ± 1.241
5.25 ± 1.826
4.0 ± 0.655
9.75 ± 5.404
6.0 ± 1.437
4.0 ± 0.255
5.5 ± 0.577
2.5 ± 0.386
5.5 ± 1.032
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here