Macaca fuscata rhadinovirus
Average proteome isoelectric point is 7.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 170 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q53CY4|Q53CY4_9GAMA JM86 OS=Macaca fuscata rhadinovirus OX=272551 GN=JM86 PE=4 SV=1
MM1 pKa = 7.27 TGSIVLALALTACLYY16 pKa = 10.97 LCLPVCATVTTSSTTGTGTPPVTTTPSAAPSVTPSFYY53 pKa = 10.66 DD54 pKa = 3.56 YY55 pKa = 11.29 DD56 pKa = 4.35 CSADD60 pKa = 3.26 TYY62 pKa = 11.31 QPVLSSFSSIWAVINSVLVAVATFLYY88 pKa = 8.44 LTYY91 pKa = 9.62 MCFFKK96 pKa = 10.81 FVEE99 pKa = 5.05 TVAHH103 pKa = 5.99 EE104 pKa = 4.0
Molecular weight: 11.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.054
IPC2_protein 4.215
IPC_protein 3.923
Toseland 3.757
ProMoST 3.999
Dawson 3.935
Bjellqvist 4.291
Wikipedia 3.91
Rodwell 3.77
Grimsley 3.681
Solomon 3.884
Lehninger 3.834
Nozaki 4.101
DTASelect 4.24
Thurlkill 3.859
EMBOSS 3.923
Sillero 4.05
Patrickios 0.006
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 4.171
Protein with the highest isoelectric point:
>tr|Q53CS8|Q53CS8_9GAMA JM142 OS=Macaca fuscata rhadinovirus OX=272551 GN=JM142 PE=4 SV=1
MM1 pKa = 7.66 GNPRR5 pKa = 11.84 IDD7 pKa = 3.48 RR8 pKa = 11.84 SHH10 pKa = 6.43 SKK12 pKa = 10.58 HH13 pKa = 6.65 VGFTLFGEE21 pKa = 4.67 SPLAGPNVPARR32 pKa = 11.84 CTWVLRR38 pKa = 11.84 NAKK41 pKa = 10.15 LPLPCRR47 pKa = 11.84 VPYY50 pKa = 10.06 SCSAIFEE57 pKa = 4.35 YY58 pKa = 10.14 TALHH62 pKa = 6.49 GWRR65 pKa = 11.84 AVGRR69 pKa = 11.84 WCANQKK75 pKa = 10.65 LMIHH79 pKa = 6.72 LLVLWLHH86 pKa = 6.29 NNTMLLILGGCFGLYY101 pKa = 9.38 KK102 pKa = 10.47 GRR104 pKa = 11.84 RR105 pKa = 11.84 KK106 pKa = 9.82 HH107 pKa = 6.05 RR108 pKa = 3.81
Molecular weight: 12.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 9.706
IPC_protein 10.452
Toseland 10.599
ProMoST 10.57
Dawson 10.716
Bjellqvist 10.452
Wikipedia 10.921
Rodwell 10.965
Grimsley 10.774
Solomon 10.818
Lehninger 10.774
Nozaki 10.643
DTASelect 10.423
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.657
Patrickios 10.716
IPC_peptide 10.818
IPC2_peptide 9.809
IPC2.peptide.svr19 8.535
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
170
0
170
45214
80
1966
266.0
29.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.761 ± 0.186
2.579 ± 0.145
4.452 ± 0.149
4.499 ± 0.142
4.401 ± 0.122
6.52 ± 0.236
2.778 ± 0.094
4.735 ± 0.148
3.846 ± 0.17
9.667 ± 0.209
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.203 ± 0.076
4.067 ± 0.129
6.44 ± 0.217
3.468 ± 0.153
6.841 ± 0.232
7.688 ± 0.206
6.586 ± 0.203
7.181 ± 0.196
1.402 ± 0.077
2.882 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here