Mycobacterium phage Panchino

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nclasvirinae; Redivirus; Mycobacterium virus Panchino

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142K7F8|A0A142K7F8_9CAUD Uncharacterized protein OS=Mycobacterium phage Panchino OX=1821537 GN=23 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 5.89LLFVPCPLCGFDD14 pKa = 3.59VAVPLLSEE22 pKa = 4.51GDD24 pKa = 3.52HH25 pKa = 5.9MRR27 pKa = 11.84APDD30 pKa = 4.57LLPNMNSHH38 pKa = 6.92VISVHH43 pKa = 5.19EE44 pKa = 4.08MDD46 pKa = 3.8VSGMM50 pKa = 3.65

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142K7G5|A0A142K7G5_9CAUD Uncharacterized protein OS=Mycobacterium phage Panchino OX=1821537 GN=30 PE=4 SV=1
MM1 pKa = 7.15RR2 pKa = 11.84TVEE5 pKa = 4.24VVDD8 pKa = 3.51AEE10 pKa = 4.31LRR12 pKa = 11.84VLAAYY17 pKa = 10.03RR18 pKa = 11.84GVSGCSVRR26 pKa = 11.84VIDD29 pKa = 4.68RR30 pKa = 11.84LLDD33 pKa = 3.4EE34 pKa = 5.15RR35 pKa = 11.84LAATRR40 pKa = 11.84NPAARR45 pKa = 11.84HH46 pKa = 4.85GARR49 pKa = 11.84LDD51 pKa = 3.65RR52 pKa = 11.84RR53 pKa = 11.84DD54 pKa = 3.49DD55 pKa = 3.42RR56 pKa = 6.31

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

14096

45

1032

213.6

23.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.124 ± 0.613

1.121 ± 0.154

6.81 ± 0.233

5.732 ± 0.301

2.887 ± 0.167

9.081 ± 0.449

1.951 ± 0.179

4.533 ± 0.185

3.079 ± 0.22

7.953 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.17

3.029 ± 0.178

5.704 ± 0.264

3.732 ± 0.237

7.002 ± 0.39

5.2 ± 0.231

6.619 ± 0.27

7.037 ± 0.238

1.923 ± 0.149

2.398 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski