Candidatus Brocadia sapporoensis

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Candidatus Brocadiae; Candidatus Brocadiales; Candidatus Brocadiaceae; Candidatus Brocadia

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2485 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V6LZR0|A0A1V6LZR0_9BACT ABC transporter domain-containing protein OS=Candidatus Brocadia sapporoensis OX=392547 GN=BIY37_07190 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84AKK4 pKa = 10.22VDD6 pKa = 3.57PDD8 pKa = 2.97ICTGCEE14 pKa = 4.06LCVQTCPDD22 pKa = 3.53VFYY25 pKa = 10.63MKK27 pKa = 10.87GDD29 pKa = 3.33VAEE32 pKa = 5.1AKK34 pKa = 10.39DD35 pKa = 3.65ADD37 pKa = 3.8VSSEE41 pKa = 4.08NEE43 pKa = 3.97DD44 pKa = 3.49LCRR47 pKa = 11.84QAAEE51 pKa = 4.17EE52 pKa = 4.71CPVEE56 pKa = 4.52AIVIIDD62 pKa = 3.32

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V6M1X7|A0A1V6M1X7_9BACT Type-4 uracil-DNA glycosylase OS=Candidatus Brocadia sapporoensis OX=392547 GN=BIY37_03745 PE=3 SV=1
MM1 pKa = 7.84PKK3 pKa = 10.57LKK5 pKa = 8.68THH7 pKa = 6.7KK8 pKa = 10.27GLKK11 pKa = 9.64KK12 pKa = 10.16RR13 pKa = 11.84IKK15 pKa = 9.83ISAKK19 pKa = 10.35GKK21 pKa = 8.73VKK23 pKa = 10.38RR24 pKa = 11.84PKK26 pKa = 10.08AGKK29 pKa = 8.66SHH31 pKa = 6.55LMSGKK36 pKa = 8.64SGRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84EE42 pKa = 4.11HH43 pKa = 5.7LRR45 pKa = 11.84KK46 pKa = 9.64KK47 pKa = 10.74AGVAPAFNKK56 pKa = 9.94IMTRR60 pKa = 11.84ALRR63 pKa = 11.84GSS65 pKa = 3.44

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2485

0

2485

783403

34

2723

315.3

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.939 ± 0.047

1.352 ± 0.02

5.219 ± 0.035

6.732 ± 0.051

4.509 ± 0.043

6.897 ± 0.046

2.178 ± 0.022

8.07 ± 0.042

7.299 ± 0.052

9.283 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.471 ± 0.022

4.267 ± 0.031

3.919 ± 0.029

3.213 ± 0.03

5.063 ± 0.036

5.967 ± 0.034

5.414 ± 0.039

6.733 ± 0.036

1.03 ± 0.019

3.445 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski