Oceanobacillus piezotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498D8X5|A0A498D8X5_9BACI DUF3231 family protein OS=Oceanobacillus piezotolerans OX=2448030 GN=D8M04_14425 PE=4 SV=1
MM1 pKa = 6.94GTSFLYY7 pKa = 10.13HH8 pKa = 4.69YY9 pKa = 10.09HH10 pKa = 6.25CLKK13 pKa = 10.18NRR15 pKa = 11.84RR16 pKa = 11.84GFDD19 pKa = 3.51EE20 pKa = 4.79VILRR24 pKa = 11.84NWKK27 pKa = 8.64WRR29 pKa = 11.84ITALISMGLVLLLAACGSGEE49 pKa = 4.1EE50 pKa = 4.49TSSEE54 pKa = 4.04EE55 pKa = 4.43NGASDD60 pKa = 4.26AAEE63 pKa = 3.91YY64 pKa = 10.92DD65 pKa = 3.94LNDD68 pKa = 3.53SYY70 pKa = 11.74TVVSDD75 pKa = 3.39NSFVPFEE82 pKa = 4.0FVEE85 pKa = 4.16NGEE88 pKa = 4.08LQGFDD93 pKa = 3.38IEE95 pKa = 4.87IINAIAEE102 pKa = 4.16EE103 pKa = 4.36AGFEE107 pKa = 4.12IAGGEE112 pKa = 4.03IEE114 pKa = 4.35TTNFDD119 pKa = 4.04GIIPGLQTGQFEE131 pKa = 4.13IAIAGISITEE141 pKa = 4.19DD142 pKa = 2.9RR143 pKa = 11.84AKK145 pKa = 10.94VIDD148 pKa = 4.03YY149 pKa = 10.44SDD151 pKa = 3.97PYY153 pKa = 9.58YY154 pKa = 10.83EE155 pKa = 4.07SGITIGVPIDD165 pKa = 3.64NEE167 pKa = 4.67DD168 pKa = 3.51IQSLEE173 pKa = 4.01DD174 pKa = 3.83LEE176 pKa = 4.84GKK178 pKa = 7.98TVATRR183 pKa = 11.84LGSTSANFIRR193 pKa = 11.84EE194 pKa = 3.99NLDD197 pKa = 3.69DD198 pKa = 3.86VTLNEE203 pKa = 4.25YY204 pKa = 9.81EE205 pKa = 4.4QLDD208 pKa = 3.93QVYY211 pKa = 9.83LAVEE215 pKa = 4.25NGSADD220 pKa = 3.32AVVYY224 pKa = 8.63DD225 pKa = 4.36APNVNYY231 pKa = 10.45YY232 pKa = 10.17ISTTGEE238 pKa = 4.18GKK240 pKa = 10.47LKK242 pKa = 10.88SIDD245 pKa = 3.75EE246 pKa = 4.64LYY248 pKa = 10.4QAEE251 pKa = 4.74DD252 pKa = 3.4YY253 pKa = 11.34GIAITKK259 pKa = 10.15GNEE262 pKa = 4.07DD263 pKa = 4.04LVSAINDD270 pKa = 3.43ALATLKK276 pKa = 10.99EE277 pKa = 4.03NGTYY281 pKa = 10.72DD282 pKa = 3.75EE283 pKa = 5.79IYY285 pKa = 10.38EE286 pKa = 4.22KK287 pKa = 10.53WFGAAEE293 pKa = 4.02EE294 pKa = 4.26

Molecular weight: 32.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498D2Z8|A0A498D2Z8_9BACI SRPBCC family protein OS=Oceanobacillus piezotolerans OX=2448030 GN=D8M04_15830 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3815

0

3815

1080255

15

1849

283.2

31.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.835 ± 0.042

0.601 ± 0.011

5.172 ± 0.035

7.885 ± 0.048

4.516 ± 0.033

6.836 ± 0.038

2.065 ± 0.022

8.353 ± 0.035

6.701 ± 0.038

9.616 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.761 ± 0.02

4.711 ± 0.025

3.562 ± 0.024

3.613 ± 0.024

3.918 ± 0.026

5.928 ± 0.032

5.389 ± 0.024

6.918 ± 0.035

0.996 ± 0.014

3.624 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski