Ammoniphilus oxalaticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Aneurinibacillus group; Ammoniphilus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3305 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A419SDH1|A0A419SDH1_9BACL SLH domain-containing protein OS=Ammoniphilus oxalaticus OX=66863 GN=BEP19_15780 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 5.05KK3 pKa = 10.72FAIQYY8 pKa = 10.57SPTGDD13 pKa = 3.75LLDD16 pKa = 3.74MEE18 pKa = 5.93GYY20 pKa = 10.09LAAPDD25 pKa = 4.48WIDD28 pKa = 3.95KK29 pKa = 10.89DD30 pKa = 3.86VLTFDD35 pKa = 4.45SEE37 pKa = 4.73AEE39 pKa = 4.15AEE41 pKa = 4.33SYY43 pKa = 10.92LYY45 pKa = 10.62DD46 pKa = 3.86QLFGTDD52 pKa = 3.79GQGLDD57 pKa = 3.95HH58 pKa = 6.73EE59 pKa = 5.23VISYY63 pKa = 10.89HH64 pKa = 6.43DD65 pKa = 3.62TLHH68 pKa = 6.65

Molecular weight: 7.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A419SJ38|A0A419SJ38_9BACL Phosphate starvation-inducible protein PhoH OS=Ammoniphilus oxalaticus OX=66863 GN=BEP19_06190 PE=4 SV=1
MM1 pKa = 7.23NRR3 pKa = 11.84TRR5 pKa = 11.84MSWMVGLGAISLAMFGWVSKK25 pKa = 10.59RR26 pKa = 11.84NRR28 pKa = 11.84SPMSKK33 pKa = 7.67MTHH36 pKa = 4.1WTNRR40 pKa = 11.84SFGWIRR46 pKa = 11.84RR47 pKa = 11.84GRR49 pKa = 11.84ITRR52 pKa = 11.84MLSRR56 pKa = 11.84MVRR59 pKa = 3.27

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3305

0

3305

923978

26

3185

279.6

31.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.715 ± 0.04

0.745 ± 0.013

5.224 ± 0.038

7.39 ± 0.054

4.301 ± 0.033

7.013 ± 0.044

2.058 ± 0.022

7.385 ± 0.041

5.781 ± 0.039

9.99 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.76 ± 0.023

3.755 ± 0.027

3.778 ± 0.029

4.541 ± 0.038

5.156 ± 0.041

5.769 ± 0.035

5.312 ± 0.028

7.017 ± 0.033

1.094 ± 0.016

3.214 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski