Aeromonas phage Asswx_1
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 407 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411B8P3|A0A411B8P3_9CAUD Uncharacterized protein OS=Aeromonas phage Asswx_1 OX=2419739 GN=ASswx1_348 PE=4 SV=1
MM1 pKa = 7.46 IFKK4 pKa = 10.28 IGQSYY9 pKa = 10.34 KK10 pKa = 8.69 IVSMFGVAVEE20 pKa = 6.06 HH21 pKa = 6.52 MDD23 pKa = 3.76 CLNDD27 pKa = 4.21 VIVCTDD33 pKa = 3.75 FDD35 pKa = 4.48 EE36 pKa = 5.47 DD37 pKa = 5.11 SGVAWFDD44 pKa = 3.12 GLMWTNFGDD53 pKa = 3.44 RR54 pKa = 11.84 GNYY57 pKa = 8.05 ILAMSDD63 pKa = 3.19 EE64 pKa = 4.87 GIEE67 pKa = 4.62 NFGTDD72 pKa = 3.19 YY73 pKa = 11.52 GSFLSNVLEE82 pKa = 4.18 EE83 pKa = 4.47 VKK85 pKa = 10.84
Molecular weight: 9.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.567
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.897
Patrickios 1.888
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A411B833|A0A411B833_9CAUD Uncharacterized protein OS=Aeromonas phage Asswx_1 OX=2419739 GN=ASswx1_128 PE=4 SV=1
MM1 pKa = 7.24 FAFTRR6 pKa = 11.84 VIFLGIIYY14 pKa = 10.01 GVGSNQCGEE23 pKa = 4.21 YY24 pKa = 9.89 HH25 pKa = 5.62 WVGTLSVTRR34 pKa = 11.84 RR35 pKa = 11.84 GLVIKK40 pKa = 10.0 YY41 pKa = 9.21 CQGNKK46 pKa = 6.56 WRR48 pKa = 11.84 VMLVTSRR55 pKa = 11.84 GLYY58 pKa = 10.51 KK59 pKa = 10.71 NIQSGVWKK67 pKa = 10.66 VLL69 pKa = 3.3
Molecular weight: 7.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.911
IPC_protein 10.394
Toseland 10.292
ProMoST 10.043
Dawson 10.496
Bjellqvist 10.233
Wikipedia 10.701
Rodwell 10.833
Grimsley 10.584
Solomon 10.54
Lehninger 10.511
Nozaki 10.321
DTASelect 10.218
Thurlkill 10.35
EMBOSS 10.687
Sillero 10.423
Patrickios 10.599
IPC_peptide 10.54
IPC2_peptide 9.326
IPC2.peptide.svr19 8.4
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
407
0
407
70352
31
1316
172.9
19.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.978 ± 0.174
1.355 ± 0.072
6.841 ± 0.11
7.181 ± 0.169
4.664 ± 0.103
6.364 ± 0.159
2.01 ± 0.065
7.174 ± 0.105
7.667 ± 0.193
6.941 ± 0.103
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.187 ± 0.082
5.551 ± 0.108
3.224 ± 0.074
2.999 ± 0.075
4.26 ± 0.077
6.543 ± 0.122
5.613 ± 0.2
7.394 ± 0.123
1.429 ± 0.045
4.627 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here