Caulobacteraceae bacterium
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4191 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q3RUV4|A0A4Q3RUV4_9CAUL Cytochrome c4 (Fragment) OS=Caulobacteraceae bacterium OX=1978230 GN=EON95_18405 PE=4 SV=1
MM1 pKa = 7.53 SGGAYY6 pKa = 9.51 GQTPAPVFTGFVGPRR21 pKa = 11.84 GEE23 pKa = 3.89 FAKK26 pKa = 10.57 RR27 pKa = 11.84 RR28 pKa = 11.84 FTRR31 pKa = 11.84 SLGLTEE37 pKa = 4.64 LCSSRR42 pKa = 11.84 VWSAGATSTWCRR54 pKa = 11.84 GGLTGLPRR62 pKa = 11.84 HH63 pKa = 5.69 TEE65 pKa = 4.07 GTDD68 pKa = 3.14 MPLSVLYY75 pKa = 10.74 YY76 pKa = 8.69 EE77 pKa = 5.58 FPATGTSGDD86 pKa = 3.91 DD87 pKa = 3.49 FMFGYY92 pKa = 10.88 ASGPGTVNTLNGGDD106 pKa = 3.67 GDD108 pKa = 4.43 DD109 pKa = 5.49 FIMGDD114 pKa = 3.51 SQNFQIAAVGNNNGSMATAQGLGDD138 pKa = 3.68 SSLWSTQNNPLIADD152 pKa = 3.74 PLIPYY157 pKa = 8.31 TYY159 pKa = 9.76 IVVEE163 pKa = 4.18 ATTGQAEE170 pKa = 4.6 YY171 pKa = 10.25 FGTPMTAGQTITVDD185 pKa = 2.79 IDD187 pKa = 3.62 FGNGSPIGTGGNVDD201 pKa = 4.64 LYY203 pKa = 11.71 VEE205 pKa = 4.39 ILDD208 pKa = 4.1 SNGTVVAFSDD218 pKa = 4.81 DD219 pKa = 3.32 SAATDD224 pKa = 3.42 GGLGSFSGLDD234 pKa = 3.31 PYY236 pKa = 11.01 LTFTAATSGSYY247 pKa = 10.01 YY248 pKa = 9.99 IRR250 pKa = 11.84 VRR252 pKa = 11.84 PLGEE256 pKa = 4.1 TTFSASSTFILNVSMTGHH274 pKa = 7.17 AVNATPIPAGADD286 pKa = 3.34 TLSGGNGDD294 pKa = 4.63 DD295 pKa = 5.53 LIVGQDD301 pKa = 3.63 GNDD304 pKa = 3.39 ILHH307 pKa = 6.85 GGSGMDD313 pKa = 3.82 ILNGGDD319 pKa = 4.24 GNDD322 pKa = 3.87 TLDD325 pKa = 4.02 GGTGGDD331 pKa = 4.04 TLNGGAGDD339 pKa = 3.97 DD340 pKa = 4.14 LVWYY344 pKa = 9.99 DD345 pKa = 3.82 PTNFDD350 pKa = 4.44 AADD353 pKa = 3.98 GGTGIDD359 pKa = 3.54 TLNTTRR365 pKa = 11.84 YY366 pKa = 10.16 ASNLVYY372 pKa = 10.88 NLASGATGFGEE383 pKa = 4.29 SLINFEE389 pKa = 4.64 NVVTGAGNDD398 pKa = 3.8 TIYY401 pKa = 10.93 GSSVANRR408 pKa = 11.84 IVTGGGNDD416 pKa = 3.47 LVLGGDD422 pKa = 3.76 GDD424 pKa = 4.56 DD425 pKa = 4.52 VISGGDD431 pKa = 3.7 GKK433 pKa = 10.79 DD434 pKa = 3.24 EE435 pKa = 5.42 LYY437 pKa = 11.12 GQGGNDD443 pKa = 3.35 TLADD447 pKa = 3.71 GGGVVAEE454 pKa = 4.42 TLLGGIGDD462 pKa = 3.85 DD463 pKa = 3.75 TYY465 pKa = 10.89 IVSFRR470 pKa = 11.84 EE471 pKa = 3.91 SSTIEE476 pKa = 3.94 YY477 pKa = 10.27 ANEE480 pKa = 4.75 GIDD483 pKa = 3.61 TVLTTFSIFALQNNVEE499 pKa = 4.32 NLTLTDD505 pKa = 3.82 NGTHH509 pKa = 6.39 GAGVGNALNNVITGGNGKK527 pKa = 9.89 DD528 pKa = 3.52 DD529 pKa = 4.01 LFGRR533 pKa = 11.84 DD534 pKa = 4.12 GDD536 pKa = 4.12 DD537 pKa = 3.48 TLDD540 pKa = 4.04 GNTGAANTLLGQLGNDD556 pKa = 3.53 TYY558 pKa = 11.23 LVRR561 pKa = 11.84 VAGDD565 pKa = 3.56 SVVEE569 pKa = 4.11 VAGQGTDD576 pKa = 3.06 TVRR579 pKa = 11.84 AFVSAHH585 pKa = 4.7 TLAANVEE592 pKa = 4.03 NLIYY596 pKa = 10.73 AGAGTFIGVGNSGGNAIYY614 pKa = 10.42 GGAQADD620 pKa = 4.4 DD621 pKa = 4.3 LNGMGGDD628 pKa = 3.87 DD629 pKa = 4.66 VIIGGAGSDD638 pKa = 4.37 FIQGGSGGDD647 pKa = 3.12 QFRR650 pKa = 11.84 YY651 pKa = 9.11 MGGEE655 pKa = 3.71 TGYY658 pKa = 11.15 DD659 pKa = 3.37 RR660 pKa = 11.84 IIDD663 pKa = 3.98 FVSGADD669 pKa = 3.23 KK670 pKa = 11.5 VMLNDD675 pKa = 4.75 AFWTQTGTVDD685 pKa = 4.3 FAQGAGAVATTGNSTFIHH703 pKa = 5.42 NTSNGMVYY711 pKa = 10.53 FDD713 pKa = 5.48 LDD715 pKa = 3.75 GNGAGLAVALCQLNVGQTLTASDD738 pKa = 3.97 FGFFF742 pKa = 4.29
Molecular weight: 75.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.554
IPC_protein 3.617
Toseland 3.363
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.592
Rodwell 3.427
Grimsley 3.274
Solomon 3.617
Lehninger 3.579
Nozaki 3.732
DTASelect 4.05
Thurlkill 3.427
EMBOSS 3.592
Sillero 3.745
Patrickios 0.744
IPC_peptide 3.617
IPC2_peptide 3.706
IPC2.peptide.svr19 3.681
Protein with the highest isoelectric point:
>tr|A0A4Q3S9T9|A0A4Q3S9T9_9CAUL 10 kDa chaperonin OS=Caulobacteraceae bacterium OX=1978230 GN=groS PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 5.3 GYY18 pKa = 7.96 RR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 9.84 NGQKK29 pKa = 9.53 IVARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.14 GRR39 pKa = 11.84 KK40 pKa = 9.04 KK41 pKa = 9.67 LTAA44 pKa = 4.2
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4191
0
4191
1190268
25
2727
284.0
30.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.781 ± 0.058
0.764 ± 0.011
6.016 ± 0.041
5.359 ± 0.038
3.508 ± 0.024
9.556 ± 0.055
1.735 ± 0.018
4.465 ± 0.028
3.045 ± 0.035
10.129 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.34 ± 0.02
2.359 ± 0.031
5.535 ± 0.035
3.032 ± 0.02
7.063 ± 0.049
4.963 ± 0.029
5.31 ± 0.043
7.422 ± 0.033
1.489 ± 0.017
2.128 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here